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All Journal Jurnal Fitopatologi Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati JURNAL AGROTEKNOLOGI Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Christanti Sumardiyono
Departemen Hama Dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada, Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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Journal : JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

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EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANG Taribuka, Johanna; Sumardiyono, Christanti; Widyastuti, Siti Muslimah; Wibowo, Arif
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 16, No 2 (2016): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (19.933 KB)

Abstract

Exploration and identification of endophytic Trichoderma in banana. Endophytic fungi Trichoderma is an organism that can used as biocontrol agent. This study aims to isolate and identify endophytic Trichoderma in roots of healthy banana plants from three districts in Yogyakarta, which will be used as biological control agent against the pathogen Fusarium oxysporum f.sp. cubense. Isolation was conducted using TSM (Trichoderma Selective Medium). We obtained six isolates of endophytic Trichoderma spp., i.e., Swn-1, Swn-2, Ksn, Psr-1, Psr-2, and Psr-3. Molecular identification was done by using ITS1 and ITS2 primer pain and sequenced. The sequence of DNA obtained was analysed and compared with NCBI database by using BLAST-N programe. The results showed that all isolates were amplified at 560-bp. Phylogenetic analysis showed that isolates Swn-1, Swn-2 and Psr-1 are homologous to Trichoderma harzianum, isolate Ksn homologous to Trichoderma asperrellum, isolate Psr-2 homologous to Trichoderma gamsii, and isolate Psr-3 homologous to Trichoderma koningiopsis, with the homologous value of 99%.
EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANG Taribuka, Johanna; Sumardiyono, Christanti; Widyastuti, Siti Muslimah; Wibowo, Arif
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 16, No 2 (2016): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.943 KB) | DOI: 10.23960/j.hptt.216115-123

Abstract

Exploration and identification of endophytic Trichoderma in banana. Endophytic fungi Trichoderma is an organism that can used as biocontrol agent. This study aims to isolate and identify endophytic Trichoderma in roots of healthy banana plants from three districts in Yogyakarta, which will be used as biological control agent against the pathogen Fusarium oxysporum f.sp. cubense. Isolation was conducted using TSM (Trichoderma Selective Medium). We obtained six isolates of endophytic Trichoderma spp., i.e., Swn-1, Swn-2, Ksn, Psr-1, Psr-2, and Psr-3. Molecular identification was done by using ITS1 and ITS2 primer pain and sequenced. The sequence of DNA obtained was analysed and compared with NCBI database by using BLAST-N programe. The results showed that all isolates were amplified at 560-bp. Phylogenetic analysis showed that isolates Swn-1, Swn-2 and Psr-1 are homologous to Trichoderma harzianum, isolate Ksn homologous to Trichoderma asperrellum, isolate Psr-2 homologous to Trichoderma gamsii, and isolate Psr-3 homologous to Trichoderma koningiopsis, with the homologous value of 99%.
Co-Authors A.A. Ketut Agung Cahyawan W Achmadi Priyatmojo Albertus Sudirman Ani Widiastuti Ani Widiastuti Aries Pratomo Arif Wibowo Arif Wibowo Arif Wibowo Arif Wibowo Arif Wibowo Bambang Hadisutrisno Christine Agustamia Didik Indradewa Dwi Kiswanti Edhi Martono Eko Agus Martanto Eko Agus Martanto Endang Semiarti Haryadi - Haryadi Haryadi Haryono Semangun Hasan Ashari Oramahi Ika Roostika Tambunan Iqna Khayatina Rusli Isam Eldeen Ibrahim Hassan Abdalla JAKA WIDADA Johanna Taribuka, Johanna Kardi Raharjo Liliek Sulistyowati Maemonah, Maemonah Miftahul Ajri Nasrun Nasrun Niken Rasmi Paramita Nur Edy Nursamsi Pusposendjojo Nursamsi Pusposendjojo, Nursamsi Peter Taylor Putri Rositasari SEDYO HARTONO Setyo Agung Sismindari Sismindari SITI MUSLIMAH WIDYASTUTI Siti Muslimah Widyastuti Siti Subandiyah Siwi Indarti Sri Trisnowati Sudarmadi Sudarmadi Sukamto Sukamto Suryanti Suryanti Suryanti Suryanti Tunjung Pamekas Wahyu Agustina Y. M.S. Maryudani Yoga Yuniadi Yufita Dwi Chinta Yufita Dwi Chinta Yuli Kristiawati