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All Journal Jurnal Fitopatologi Indonesia Pembangunan Pedesaan Prosiding AGRIVITA, Journal of Agricultural Science Jurnal Agro Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Pengabdian Pada Masyarakat Jurnal SOLMA Agrin : Jurnal Penelitian Pertanian Biosaintifika: Journal of Biology & Biology Education JURNAL PENGABDIAN KEPADA MASYARAKAT Abditani : Jurnal Pengabdian Masyarakat Menara Perkebunan JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) Agrotechnology Research Journal Jurnal Agro Wiralodra SINTA Journal (Science, Technology, and Agricultural) JURNAL AGROSAINS : Karya Kreatif dan Inovatif Media Pertanian

Endang Mugiastuti

Fakultas Pertanian Universitas Jenderal Soedirman Purwokerto

Author-ID : 322935

Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Law, Crime, Criminology & Criminal Justice Library & Information Science Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Raw Secondary Metabolites of Trichoderma harzianum T10 in Tapioca Flour Towards Cucumber Damping-off Soesanto, Loekas; Ilahiyyah, Hidayatul; Mugiastuti, Endang; Manan, Abdul; Rostaman, Rostaman
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.24567

Trichoderma harzianum is effective for controlling soil-borne pathogenic fungi and producing secondary metabolites. When applied in the field, the raw secondary metabolites are quickly decreased directly by sunlight. One strategy to avoid degradation is the use of tapioca fluor liquid formula for biological control agents. This research aimed to obtain the most effective concentration of tapioca flour in development of raw secondary metabolites of Trichoderma harzianum T10, its effect on damping-off and growth of cucumber. This research was carried out at the screen house and the Plant Protection Laboratory, Faculty of Agriculture, Jenderal Soedirman University from September 2017 up to January 2018. The study was conducted in two stages, i.e., in vitro and in planta. The in vitro stage used completely randomized design with five repetitions and five treatments consisted of T. harzianum T10 in Potato Dextrose Broth, and in 0.5; 1; 1.5; and 2% of tapioca flour media. In in planta, randomized block design was used with five repetitions and six treatments consisted of control, T. harzianum T10 in PDB, and in 0.5; 1; 1.5, and 2% of tapioca flour media. Variables observed were density of conidia, disease incubation period, disease incidence, AUDPC, maximum growth potential, germination ability, plant height, canopy fresh weight, root length, and fresh root weight. Result of the research showed that the highest conidial density (1.23 x 107 conidia mL-1) of T. harzianum T10 was found in 2% tapioca flour with an increase of 63.28% compared to the PDB. The tapioca flour of 1 and 2%, and PDB could suppress the disease incidence by 81.82%. The lowest AUDPC was at 2% tapioca flour. The raw secondary metabolites could not delay the incubation period significantly and increase cucumber plant growth.Â The novelty is the use of antagonistic fungi in terms of raw secondary metabolites and the discovery of tapioca flour with the right concentration to produce high conidia density and high raw secondary metabolites. TheÂ benefits are to find other cheaper ingredients in promoting antagonistic fungal growth and the use of antagonistic fungal bioactive compounds to control plant pathogen

Raw Secondary Metabolites of Chitosan-enriched Pseudomonas fluorescens P60 to Control Corn Sheath Blight Soesanto, Loekas; Pradiptha, Clarissa Nadya; Mugiastuti, Endang
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.28775

Corn is a strategic cereal with economic value and always face the corn sheath blight in the field. An alternative safely and environmentally friendly disease control is the use of biological agent Pseudomonas fluorescens P60 that produced raw secondary metabolites. This study aimed to test the effectiveness of chitosan enriched P. fluorescens P60 raw secondary metabolites against leaf blight and to determine the effect on the growth of maize. The research was conducted at the Laboratory of Plant Protection and Experimental Farm, Faculty of Agriculture, Jenderal Soedirman University. In vitro test used a completely randomized design and in planta test used a randomized block design consisted of four treatments and six replicates. The treatments consisted of control and the secondary metabolites of P. fluorescens P60 enriched with chitosan 1, 2, and 3%. Variables observed were inhibition ability, chitinase and protease analysis qualitatively, incubation period, disease intensity, infection rate, AUDPC, crop height, crop fresh weight, and root fresh weight. The results showed that the secondary metabolites of P. fluorescens P60 enriched with chitosan 3% was effective in inhibiting the growth of Rhizoctonia solani as 63.3%, produced chitinase and protease, and could control the disease by delaying the incubation period as 79.05%, lowering the disease intensity as 68.68%, lowering the infection rate as 100%, and lowering AUDPC as 83.32%. The secondary metabolites of P. fluorescens P60 enriched with chitosan 3% was effective in improving plant growth by increasing plant fresh weight as 33.9% and root fresh weight as 43.09% compared to control. Chitosan could be used for improving effectiveness of antagonistic bacteria raw secondary metabolites. The raw secondary metabolites of bacterial antagonists could be improved their effectiveness against plant diseases by enrichment of chitosan.

CROSS APPLICATION OF ENTOMOPATHOGENIC FUNGI RAW SECONDARY METABOLITES FOR CONTROLLING FUSARIUM WILT OF CHILI SEEDLINGS Soesanto, Loekas; Sari, Lintang Yunita; Mugiastuti, Endang; Manan, Abdul
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 2 (2021): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.22182-90

Cross application of entomopathogenic fungi raw secondary metabolites for controlling fusarium wilt of chili seedlings. Theresearch aimed to determine the effect of entomopathogenic fungi raw secondary metabolites on fusarium wilt on chili plants and on growth of chili. In vitro test used a Completely Randomized Design with 5 treatments and 5 replicate and in planta using a Randomized Block Design with 5 treatments and 5 replicatie including control, secondary metabolites of Beauveria bassiana B10, B. bassiana B16, Metarhizium anisopliae M16, dan Lecanicillium lecanii L16. Variables observed included inhibition ability, incubation period, desease intensity, plant height, root length, and phenolic compounds (tannins, saponin, and hydroquinone) content qualitatively. The results showed that secondary metabolites of B. bassiana B10, B. bassiana B16, M. anisopliae M16, and L. lecanii L16 were able to inhibit growth of Fusarium oxysporum f.sp. capsici by 50.62; 50,64; 48,62; 56.62%, respectively, extend incubation periods of 71.05; 73,38; 64.89; and 68.57%, respectively, suppress disease intensity by 99.99; 99.99; 99.99; and 99.99%, respectively, can increase plant height by 15.22; 18.8; 21.14; 21.69%, respectively, increasing the root length by 22.61; 25,71; 26,34; 33.50%, respectively, and can increase the content of tannins, saponins and hydroquinone compounds qualitatively compared to controls. The secondary metabolites of enthomopathogenic fungi could be used as organic control for soilborne pathogenic fungi.

Ketahanan Tiga Varietas Jahe terhadap Fusarium oxysporum f. sp. zingiberi in Vitro dan in Planta Dyah Pancasiwi; Soedarmono Soedarmono; Endang Mugiastuti; Loekas Soesanto
Jurnal Fitopatologi Indonesia Vol 9 No 2 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Fusarium oxysporum f. sp. zingiberi is the causal agent of dry rot on ginger and could highly effect ginger production. Resistant variety is one of disease control strategy that is recommended. Various ginger varieties are available but their response to F. oxysporum f. sp. zingiberi has not been reported. In this study three ginger varieties were evaluated for their resistence to F. oxysporum f. sp. zingiberi based on in vitro and in planta inoculalation method. Gadjah and Emprit varieties were considered moderately susceptible whereas Merah varieties was moderately resistance to F. oxysporum f. sp. zingiberi. pathogen will be indicated.Key words: dry rot disease, ginger var. Merah, resistant variety, resistance

Aplikasi Formula Cair Pseudomonas fluorescens P60 untuk Menekan Penyakit Virus Cabai Merah Loekas Soesanto; Endang Mugiastuti; Ruth Feti Rahayuniati
Jurnal Fitopatologi Indonesia Vol 9 No 6 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Viral diseases of chilli pepper are difficult to control, therefore the use of Pseudomonas fluorescens P60 should be evaluated. The aims of this research were to determine the influence of liquid formula of P. fluorescens P60 on virod disease and on growth and yield of chili pepper. Randomized block design (RBD) experiment was composed of 7 treatments and 4 replicates, i.e., control, insecticide applicaton, P. fluorescens P60 application by seedling drenching and spraying for 1, 2, 3, 5, and 7 times. The result showed that 5 times application of P. fluorescens P60 by drenching and spraying was able to suppress viral disease and reduce disease intensity by to 73.37%, increasing density level of P. fluorescens P60 to 9.50 x 1011 and increase phenolic compounds (saponin, tannin and glycoside). The same treatment could increase plant height 23.7%, root lenght 6.44%, plant dry weight 66.68%, root dry weight 23.59%, and yield weight 53.16%.

Kemampuan Campuran Bacillus sp., Pseudomonas fluorescens, dan Trichoderma sp. untuk Mengendalikan Penyakit Layu Bakteri pada Tanaman Tomat abdul manan; Endang Mugiastuti; Loekas Soesanto
Jurnal Fitopatologi Indonesia Vol. 14 No. 2 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Ability of Bacillus sp., Pseudomonas fluorescens, and Trichoderma sp. to Control Tomato Bacterial Wilt DiseaseRalstonia solanacearum is known as the causal agent of bacterial wilt on tomato. The bacteria may infect all stadia of plant growth and decrease tomato production. Biological control using antagonistic microbes is considered as a potential control alternative for the disease. This research was aimed to assay the ability of combination treatment of Bacillus sp., Pseudomonas fluorescens, and Trichoderma sp. in controlling R. solanacearum and its effect on growth and yield of tomato in the field. The treatments consisted of control (without antagonistic microbes), mixed combination of Bacillus sp. B8 + Bacillus sp. B11 + Trichoderma sp.; Bacillus sp. B8 + Pseudomonas flourescens P8 + Trichoderma sp.; and Streptomisin sulfat 20%. Antagonistic microbes was applied at planting time, as much as 100 mL per plant. The result showed that mixed combination of Bacillus sp. B8 + Bacillus sp. B11 + Trichoderma sp. was the best treatment in controlling the disease as indicated by delaying incubation period up to 6.2 days, decreasing disease incidence up to 12.3%, increasing plant growth up to 42.80%, and increasing yield up to 14.99%.

Effect of Four Liquid Organic Wastes on The Growth of Four Trichoderma harzianum Isolates and Their Effect on Cucumber Growth and Yield Loekas Loekas Soesanto Soesanto; Alvin Adhyatputera Ramadhana; Endang Mugiastuti; Abdul Manan
SINTA Journal (Science, Technology, and Agricultural) Vol 2, No 2 (2021)
Publisher : Perkumpulan Dosen Muda (PDM) Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37638/sinta.2.2.19-30

The aim of this research was to determine the effect of fourliquid organic substrates on the growth of four Trichoderma harzianum isolates and their effect on cucumber growth and yield. Randomized block design was used with 20 treatments and 3 replicates. The treatments consisted of control, tofu liquid waste, rice washing water, coconut water, and tapioca waste substrates each combined with four T. harzianum isolates. Variables observed were conidia density during decomposition, conidia late density, crop height, root length, root fresh and dry weight, crop fresh and dry weight, the first flowering, number of fruits per plant, and fruit weight. Result of the research showed that in the tapioca waste substrate, only T16 isolates was able to grow well with a maximum density of 6,70x107 conidia/mL. In the rice washing water substrate, conidia growth of the isolate was better than coconut water substrate with a maximum density of 6,25x107 conidia/mL. The best liquid organic substrate for growing media of T. harzianum was tofu liquid waste. On the 4th day with the tofu waste substrate, T16 isolate could achieve conidia density of 1,12x108 conidia/mL. The liquid organic substrate resulted from T. harzianum decomposition was not different on cucumber yield.

Aplikasi Bacillus sp. untuk mengendalikan penyakit layu fusarium pada tanaman tomat Endang Mugiastuti; Abdul Manan; Ruth Feti Rahayuniati; Loekas Soesanto
Jurnal Agro Vol 6, No 2 (2019)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/5397

Fusarium oxysporum merupakan penyebab penyakit layu fusarium yang dapat menurunkan produksi tomat. Pengendalian hayati dengan menggunakan bakteri antagonis seperti Bacillus sp. merupakan alternatif pengendalian yang potensial dan ramah lingkungan. Penelitian bertujuan untuk mengetahui kemampuan Bacillus sp. dalam mengendalikan penyakit layu fusarium pada tanaman tomat di lapangan. Percobaan menggunakan rancangan acak kelompok dengan 5 perlakuan dan 5 ulangan, meliputi: kontrol, Bacillus sp. B42, Bacillus sp. B64, gabungan Bacillus sp. B42 dan B64, serta fungisida. Hasil pengujian menunjukkan bahwa Bacillus sp. B.64 merupakan bakteri antagonis terbaik dalam menekan intensitas penyakit layu fusarium, karena dapat menunda masa inkubasi 15,76%, menekan intensitas penyakit 38,77%, meningkatkan kandungan fenol tanaman (tanin, saponin dan glikosida), serta meningkatkan pertumbuhan dan hasil tanaman dengan meningkatkan bobot kering tajuk 32,05%, bobot kering akar 15,23%, dan bobot buah per tanaman 46,48%.ABSTRACTFusarium oxysporum is the causal agent of fusarium wilt disease which decreases the production of tomatoes. Biological control managenement using bacterial antagonists is a potential alternative to prevent the infection of the disease. The aim of this research was to determine the ability of Bacillus sp. to control tomato fusarium wilt in the field. Randomized block design (RBD) experiment was used consisting of 5 treatments and 5 replications i.e. control, Bacillus sp. B42, Bacillus sp. B64, combination of Bacillus sp B42 + B64, and fungicide. The results showed that Bacillus sp. B64 was the best bacterial antagonist agent to control tomato wilt disease by delaying incubation period (15.76%), decreasing disease intensity (38.77%), increasing phenol compounds (tannin, saponin, glycosides) and improving plant growth and yield. Furthermore, the results showed the increasement of shoot dry weight to 32.05%, root dry weight to 15.23%, and yield to 46.48% as well.

Aplikasi Pseudomonas fluorescens P20 formula cair tepung ikan terhadap rebah semai mentimun Hening Kurniasih; Nur Prihatiningsih; Endang Mugiastuti; Loekas Soesanto
Jurnal Agro Vol 7, No 2 (2020)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/8591

Rebah semai merupakan penyakit penting bibit mentimun, dan formula cair Pseudomonas fluorescens P20 dengan tepung ikan perlu dicoba untuk mengatasi hal tersebut. Penelitian bertujuan mencari konsentrasi tepat tepung ikan untuk P. fluorescens P20, pengaruhnya dalam menekan rebah semai, dan terhadap pertumbuhan mentimun. Penelitian dilaksanakan di Screen House Fakultas Pertanian Universitas Jenderal Soedirman, Purwokerto selama empat bulan. Rancangan Acak Lengkap digunakan pada in vitro dengan 5 ulangan dan 5 perlakuan terdiri atas kontrol (King’s B cair), serta tepung ikan 10, 20, 30, dan 40 g L-1. Uji in planta menggunakan Rancangan Acak Kelompok dengan 5 ulangan dan 6 perlakuan terdiri atas kontrol, King’s B cair, serta tepung ikan 10, 20, 30, dan 40 g L-1. Variabel yang diamati adalah populasi bakteri, masa inkubasi, kejadian penyakit, area under the disease progress curve (AUDPC), tinggi tanaman, panjang akar, bobot tanaman segar, dan bobot akar segar. Hasil penelitian menunjukkan bahwa P. fluorescens P20 dalam tepung ikan 10 g L-1 memiliki populasi tertinggi yaitu 3,99 x 1021 upk mL-1 atau meningkat sebesar 47,23%. Saat diaplikasikan, formula bakteri ini paling efektif menunda masa inkubasi dan menekan kejadian penyakit hingga 100% dan nilai AUDPC terendah yaitu 0%-hari. Perlakuan mampu meningkatkan panjang akar 5,30-31,19% dan bobot akar segar 10,81-65,85%. Damping-off is an important cucumber seedlings disease, and liquid formulation of Pseudomonas fluorescens P20 is developed to overcome this problem. This research aimed to gain the right concentration of fish flour for P. fluorescens P20, its effect on suppressing damping-off and on cucumber growth. The research was conducted at the Screen House, Faculty of Agriculture, Jenderal Soedirman University, Purwokerto for four months. Randomized completely design was used for in vitro test with 5 treatments consisted of control, fish flour of 10, 20, 30, and 40 g L-1repeated 5 times. Randomized block design was used for in planta test with 6 treatments consisted of control, King’s B Broth, flour liquid of 10, 20, 30, and 40 g L-1repeated 5 times. Variables observed were bacterial population, incubation period, disease incidence, area under the disease progress curve (AUDPC), plants height, roots length plants fresh weight, and roots fresh weight. Result showed that P. fluorescensP20 in fish flour of 10 g L-1 had the highest population as 3.99 x 1021 cfu ml-1 or increase as 47.23%. Application of this formula could delay incubation period and suppress disease incidence as 100% and decrease AUDPC as 0%-days. The formula could increase roots length and roots fresh weight as 5,30-31,19 and 10,81-65,85%.

Pemanfaatan Beberapa Kaldu Hewan sebagai Bahan Formula Cair Pseudomonas fluorescens P60 untuk Mengendalikan Sclerotium rolfsii pada Tanaman Mentimun Loekas Soesanto; Endang Mugiastuti; Ruth Feti Rahayuniati
Jurnal Perlindungan Tanaman Indonesia Vol 17, No 1 (2011)
Publisher : Universitas Gadjah Mada

A research aiming at knowing the potency of several animal broths as organic liquid formula of Pseudomonas fluorescens P60, soaking period of Sclerotium rolfsii sclerotia, and its application method on cucumber stem-end rot was done. Completely randomized design and randomized block design both arranged by factorial were used for in vitro and in planta tests, respectively. The first factor was six kinds of animal broth, i.e., golden snail, local chicken,broiler chicken, catfish, cow bone, and rat. The second one for in vitro test was the soaking period in the Pseudomonas fluorescens P60 formula, i.e., 0, 1, 10, and 100 hours and for in planta one was application methods, i.e., seed soaking or crop spraying. Result of the research showed that the best animal broth as liquid formula for Pseudomonas fluorescens P60 was golden snail broth indicated by suppression of sclerotial germination up to 97.4% after soaking for 100 hours. The best application method to suppress the disease was spraying method showed by suppressed of sclerotial germination, longer incubation period, and suppressed disease incidence and sclerotial late population of 55.79, 147.35, 66.67, and 59.68%, repectively. Spraying the formula could also increase crop height difference, fresh and dry weight of crop, fresh and dry weight of root, and root length to 146.83, 86.62, 112.5, 87.88, 140, and 159.68%, respectively.

Co-Authors Abdul Manan Abdul Manan abdul manan Abdul Manan Abdul Manan Abdul Manan Abdul manan manan Agus Dana Permana dan Rostaman ., Agus Dana Permana dan Rostaman Agus Suroto Agus Suyanto Alvin Adhyatputera Ramadhana Anang Khairul Rahman Anida Amalia Rahma DARINI SRI UTAMI Dyah Pancasiwi Eka Oktaviani Eka Oktaviani Elvina Hapsari Etik Wukir Tini Fenty Chakimatul Isnaeni Fida Suci Ersapoetri Fitrianti Fitrianti Hening Kurniasih Hidayatul Ilahiyyah Ilahiyyah, Hidayatul Khoeruriza, Khoeruriza Kurniasih Mucharomah Kustam, Kustam Loekas - SOESANTO Loekas Loekas Soesanto Soesanto Loekas Soesanto Loekas Soesanto Loekas Soesanto Loekas Soesanto Loekas Soesanto Loekas Soesanto Loekas Soesanto manan, Abdul manan Muhammad Bahrudin Muljo Wachjadi Muljo Wachjadi Murti Wisnu Ragil SASTYAWAN Ni Wayan Anik Leana Nur Prihatiningsih Nurtiati Nurtiati Nurtiati, Nurtiati Pradiptha, Clarissa Nadya Prasmaji Sulistyanto Purwanto Purwanto Rostaman Rostaman Ruth Feti Rahayuniati Ruth Feti Rahayuniati Ruth Feti Rahayuniati Ruth Feti Rahayuniati Ruth Feti Rahayuniati Ruth Feti Rahayuniati Ruth Feti Rahayuniati Ruth Feti Rahayuniati Sari, Lintang Yunita Slamet Rohadi Soedarmono Soedarmono Susamto Somowiyarjo Tarjoko Tarjoko Y. M. Sugi Maryudani

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