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Uji Lapangan Formula Cair Pseudomonas fluorescens P60 terhadap Layu Fusarium pada Tanaman Tomat Loekas Soesanto; Endang Mugiastuti; Ruth Feti Rahayuniati; Abdul Manan
Jurnal Perlindungan Tanaman Indonesia Vol 17, No 2 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3043.642 KB) | DOI: 10.22146/jpti.9830

Abstract

A research aimed at knowing 1) the effect of Pseudomonas fluorescens P60 in liquid formula on Fusarium wilt of tomato, 2) the effect of P. fluorescens P60 in the formula on tomato growth and yield, and 3) P. fluorescens P60 mechanisms on tomata was carried out at tomato field of Selomoyo Village, Kaliangkrik Subdistrict, Magelang Regency at altitude of 826 m above sea level. Randomized block design was used with seven treatments and four replicates. The treatments were control, with P. fluorescens P60 soaked for 15 min and without fungicide, pathogen without P. fluorescens P60 with fungicide (PBG1), pathogen with P. fluorescens P60 without fungicide, pathogen with pouring P. fluorescens P60 1, 3, and 5 times. Result indicated that application of formulated P. fluorescens P60 for 5 times decreased the disease intensity as high as 26.77%, and late population of the pathogen but increased P. fluorescens P60 as high as 4.54×1010 cfu ml-1. P. fluorescens P60 affected growth and yield of tomato. P. Fluorescens P60 induced tomato resistance by increasing qualitatively its phenolic compound content (saponin, tannin, glycoside). Penelitian dengan tujuan untuk mengetahui: 1) pengaruh Pseudomonas fluorescens P60 dalam formula cair terhadap penyakit layu fusarium pada tanaman tomat, 2) pengaruh P. fluorescens P60 dalam formula cair terhadap pertumbuhan dan produksi tanaman tomat, dan 3) mekanisme P. fluorescens P60 pada tanaman tomat dilakukan di lahan Desa Selomoyo, Kecamatan Kaliangkrik, Kabupaten Magelang dengan ketinggian 826 m di atas permukaan laut. Penelitian menggunakan Rancangan Acak Kelompok (RAK), dengan 7 perlakuan dan jumlah ulangan 4 kali, dan setiap unit terdiri atas 8 tanaman. Perlakuan tersebut meliputi kontrol; dengan P. fluorescens P60 rendam 15 menit dan tanpa fungisida; dengan patogen; tanpa P. fluorescens P60; dengan fungisida (PBG1); patogen, tanpa P. fluorescens P60, tanpa fungisida; patogen, dengan penyiraman P. fluorescens P60 1 kali; patogen, dengan penyiraman P. fluorescens P60 3 kali; dan patogen, dengan penyiraman P. fluorescens P60 5 kali. Pemberian P. fluorescens P60 selama 5 kali memberikan pengaruh sangat nyata dalam menekan penyakit layu fusarium yang disebabkan Fusarium oxysporum. Hal ini ditunjukkan pada penurunan intensitas penyakit sebesar 26,77%, rendahnya kepadatan akhir F. oxysporum serta tingginya nilai kepadatan P. fluorescens P60 sebesar 4,54×1010 unit pembentuk spora/ml. Pengaruh pemberian P. fluorescens P60 belum menunjukkan pengaruh nyata pada komponen pertumbuhan dan hasil. P. fluorescens P60 mampu mengimbas ketahanan tanaman tomat dengan meningkatkan kandungan senyawa fenol (saponin, tanin, glikosida).
Pemanfaatan Enzyme-Linked Immunosorbent Assay untuk Mengukur Titer Virus dalam Bawang Putih Susamto Somowiyarjo; Endang Mugiastuti; Y. M. Sugi Maryudani
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 1 (2000)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12407

Abstract

Non-precoated Indirect ELISA had been developed by employing monoclonal antibodies against virus isolated from Sangga variety of garlic. The ELISA was used to measure the titer of virus in the plant. In comparation with biological assay using Chenopodium amaranticolor, ELISA was able to measure the virus titer faster and was more simpler. The highest titer of virus was obtained using the first leaf of garlic at age of 29-36 days after planting. Application of nitrogen at high dose and high temperature of garlic cultivation trends to increase the virus titer. The results of this experiment may be used to improve the method of sampling to detect virus in garlic tissues.
ABILITY TEST OF SEVERAL ANTAGONISTS TO CONTROL POTATO BACTERIAL WILT IN THE FIELD Loekas Soesanto; Abdul Manan; Muljo Wachjadi; Endang Mugiastuti
AGRIVITA, Journal of Agricultural Science Vol 35, No 1 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i1.227

Abstract

The research objective was to know ability of antagonistic microbes to control bacterial wilt on potato in the field. This research was carried out at Serang Village, Karangreja Subdistrict, Purbalingga Regency from June up to August 2012. The antagonist, originally isolated from potato field, was Bacillus sp. B2 and B4, and Pseudomonas sp. P19 and P20. Based on the research result, Pseudomonas P19 could control the disease on potato with delaying incubation period of 78.95%, suppressing disease intensity of 51.57%, decreasing final pathogenic population of 99.74%, and inducing plant resistance with increasing saponin, tannin, and glycoside content. However, the antagonist could not increase growth and yield of potato.  Keywords: antagonistic microbes, bacterial wilt, potato
Aplikasi metabolit sekunder dari tiga isolat Pseudomonas fluorescens untuk mengendalikan penyakit antraknosa pada daun kakao Fitrianti FITRIANTI; Loekas - SOESANTO; Endang MUGIASTUTI; Murti Wisnu Ragil SASTYAWAN; Abdul MANAN
E-Journal Menara Perkebunan Vol 90, No 1 (2022): April, 2022
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v90i1.483

Abstract

Anthracnose caused by Colletotrichum gloeosporoides is an important disease in cocoa. This research aimed to determine the effectiveness of secondary metabolites derived from three isolates of Pseudomonas fluorescens to control cocoa leaves anthracnose, and their influence on the growth of cocoa plants. The research was conducted at a smallholder cocoa plantation in Putat Village, Patuk District, Gunung Kidul Regency, Yogyakarta for four months. A randomized block design was used with four replicates and seven treatments consisted of control, application of secondary metabolites from P. fluorescens P60, P. fluorescens P20, P. fluorescens P8, combination P. fluorescens P60 + P20, P. fluorescens P60 + P8, and P. fluorescens P20 + P8. Variables observed were disease intensity, infection rate, number of healthy shoots and qualitative phenolic compound. Resultsof the research showed that the secondary metabolites of P. fluorescens P60, P20 and P8 alone or in combination  suppressed the disease intensity by42.01-54.50%. The infection rate caused by metabolite secondary of P. fluorescens P60, P20, P8, P. fluorescens P60+P20, P. fluorescens P60+P8,and P. fluorescens P20+P8 was 0.23; 0.25; 0.26; 0.26; 0.31; and 0.24 units/day, respectively. The secondary metabolites of P. fluorescens P60 increased the number of healthy shoots by 67.44 %. The secondary metabolites of P. fluorescens P60 increased phenolic compounds (tannin, saponin, and glycosides) in cocoa leaves.[Keyword: leaves anthracnose, Colletotrichum gloeosporoides, cocoa, secondary metabolites, Pseudomonas fluorescens] AbstrakAntraknosa yang disebabkan oleh Colletotrichum gloeosporioides merupakan penyakit penting pada tanaman kakao. Penelitian bertujuan mengkaji keefektifan metabolit sekunder dari tiga isolat Pseudomonas fluorescens untuk mengendalikan penyakit antraknosa pada daun kakao, serta pengaruhnya terhadap pertumbuhan tanaman kakao. Penelitian dilaksanakan di perkebunan kakao rakyat, Desa Putat, Kecamatan Patuk, Kabupaten Gunung Kidul, Yogyakarta selama empat bulan. Penelitian menggunakan rancangan acak kelompok untuk menguji 7 perlakuan yang diulang 4 kali, perlakuan yang diuji adalah kontrol, aplikasi metabolit sekunder P. fluorescens P60, P. fluorescens P20, P. fluorescens P8, kombinasi P. fluorescens P60+P20, P. fluorescens P60+P8, dan P. fluorescens P20+P8. Variabel pengamatan meliputi intensitas penyakit, laju infeksi, jumlah tunas sehat, dan kandungan senyawa fenol secara kualitatif. Hasil penelitian menunjukkan bahwa perlakuan metabolit sekunder P. fluorescens P60, P20, dan P8 secara tunggal dan gabungan mampu menekan intensitas penyakit berkisar 42,01-54,50%. Laju infeksi perlakuan metabolit sekunder P. fluorescens P60, P. fluorescens P20, P. fluorescens P8, kombinasi P. fluorescens P60+P20, P. fluorescens P60+P8 dan P. fluorescens P20+P8 berturut-turut 0,23; 0,25; 0,26; 0,26; 0,31; dan 0,24 unit/hari. Metabolit sekunder P. fluorescens P60 meningkatkan jumlah tunas sehat sebesar 67,44%. Perlakuan P. fluorescens P60 meningkatkan senyawa fenol (saponin, tanin, dan glikosida) pada daun kakao.[Kata kunci: antraknosa daun, Colletotrichum gloeosporoides, kakao, metabolit sekunder, Pseudomonas fluorescens]
Penerapan Teknologi Pengendalian Penyakit Tanaman Yang Ramah Lingkungan Pada Tembakau Endang Mugiastuti; Loekas Soesanto; Abdul Manan
JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) VOL. 2 NOMOR 2 SEPTEMBER 2018 JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat)
Publisher : Lembaga Publikasi Ilmiah dan Penerbitan (LPIP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (908.003 KB) | DOI: 10.30595/jppm.v2i2.1793

Abstract

Tobacco is one of the most important plants which were cultivated by Farmers Groups "Darma Mukti Tani I" and "Karya Tani" of Wangon District, Banyumas Regency. However, several obstacles often interfere with the production of tobacco, including quite high tobacco pests and diseases. Socialization of  plant pests diseases management by using Trichoderma sp. and Pseudomonas fluorescens biopesticides, needs to be done. The activity aimed was to increase knowledge and skills of farmers on pests and diseases in tobacco and their management measures with biological pesticides. Issues addressed farmers partners with several technology solution, which was conducted by the transfer of technology through education, training, demonstration plots, and mentoring. Dissemination activities were able to increase their knowledge of pests and diseases, their control, and the control of environmental friendly as 58,82, 62,5, and 67.67%, respectively. The training activities of P. fluorescens exploration was able to improve their ability as 166.67%. Propagation activity and application training could improve their ability for 100 and 73,33%, respectively. The training activities of Trichoderma sp. exploration were able to improve their ability as 94,44%, while the propagation and application training could improve their ability, respectively, as 73,33 and 88.23%. Application of biological pesticides P. fluorescens and Trichoderma sp. at demonstration plot was able to increase growth and reduced the intensity of bacterial and fusarium wilt disease by 40-50%.Keywords: Tobacco, technology transfer, pests and diseases, biological control.
Pelatihan Teknologi Budidaya dan Pengelolaan Penyakit Ramah Lingkungan Pada Tanaman Lada Endang Mugiastuti; Loekas Soesanto; Abdul Manan
JURNAL PENGABDIAN KEPADA MASYARAKAT Vol 8, No 2 (2018): Desember 2018
Publisher : LPPM UNINUS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (235.614 KB) | DOI: 10.30999/jpkm.v8i2.279

Abstract

Pepper is an important commodity and has high economic value. This commodity has begun to be cultivated by members of the PKK RT 07 and PKK RT 09, Tambak Sogra Village, Sumbang District, Banyumas Regency, in an effort to increase the use of the yard and increase family income. However, the knowledge and skills of PKK members regarding cultivation and diseases of pepper plants as well as effective and environmentally friendly management efforts are very low. The activity aimed was to improve knowledge and skills of members of the PKK group in the cultivation and management of pepper disease that are effective and environmentally friendly. Activities carried out with dissemination, training, and technology demonstration plots. The activities were able to increase the knowledge and skills of members of the PKK group in pepper cultivation and the management of the disease which is environmentally friendly. In addition, the application of Trichoderma sp. and Pseudomonas fluorescens in the demonstration plot can increase the growth of pepper.
Sosialisasi dan Pelatihan Pemanfaatan Biopestisida Mikroba Antagonis Campuran untuk Mengendalikan Penyakit Bawang Merah di Musim Hujan Abdul Manan; Endang Mugiastuti; Loekas Soesanto
Jurnal Pengabdian Pada Masyarakat Vol 6 No 1 (2021)
Publisher : Universitas Mathla'ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30653/002.202161.630

Abstract

SOCIALIZATION AND TRAINING ON THE UTILIZATION OF MIXED ANTAGONISTIC MICROBIAL BIOPESTICIDES TO CONTROL SHALLOTS IN THE RAINY SEASON. The constraints on shallot cultivation in the rainy season are the more severe attacks of Fusarium oxysporum (Fusarium wilt) and Alternaria porii (purple disease). Solid formula of Trichoderma sp., Bacillus sp. B8, and Bacillus sp. B11 have been proven to increase their effectiveness and also cultivation technique and environmental modification. However, the benefit of the technology did not informed members of Farmers Group in Tambaksogra Village, Sumbang Regency, Banyumas District yet. The objectives of the activity are to increase farmers’ knowledge and skills on shallot cultivation technique out of the season, to multiply and to apply the antagonistic microbes solid mixed formula, to increase the crop production quantity and quality, and to increase farmers’ income. The method used was transfer of the technology through education, training, and demplot. The activities carried out were socialization of the potency and application of shallot cultivation technique out the season, multiplication and application of the antagonistic microbial solid mixed formula with the use of available media at the location, demonstration plot of the shallot cultivation technology with the application of the solid mixed formulation compared to single antagonistic, and farmers’ accompaniment in implementing the technology package. Result showed that there was an increase in farmers’ knowledge on the potency and application of solid formula biopesticides by 69.61%, as well as an increase skill in making solid formula biopesticide and its application by 64.33%.
PENGUJIAN KEMAMPUAN MIKROBA ANTAGONIS UNTUK MENGENDALIKAN PENYAKIT HAWAR DAUN DAN LAYU BAKTERI PADA TANAMAN KENTANG DI DAERAH ENDEMIS Muljo Wachjadi; Loekas Soesanto; Abdul Manan; Endang Mugiastuti
Agrin Vol 17, No 2 (2013): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2013.17.2.202

Abstract

Penelitian bertujuan untuk mengetahui kemampuan beberapa mikroba antagonis untuk mengendalikanpenyakit hawar daun dan layu bakteri pada tanaman kentang di daerah endemik. Penelitian ini dilaksanakan dilahan kentang Desa Kejajar, Kecamatan Kejajar, Kabupaten Wonosobo, pada bulan Juni sampai Agustus 2013.Mikroba antagonis yang digunakan hasil isolasi dari pertanaman kentang dan telah diuji di rumah kaca danlapangan terbatas, yaitu dengan Bacillus sp. B2 dan B4, serta Pseudomonas sp. P19 dan P21. Berdasarkan hasilpenelitian yang telah dilaksanakan, dapat disimpulkan bahwa mikroba antagonis belum mampu mengendalikanpenyakit hawar daun dan layu bakteri, serta belum mampu meningkatkan pertumbuhan dan hasil tanamankentang. Akan tetapi, mikroba antagonis mampu mengimbas senyawa tanin pada tanaman kentang.Kata kunci: mikroba antagonis, penyakit hawar daun, layu bakteri, kentangABSTRACTThe research aimed at knowing ability of some antagonistic microbes to control leaf blight and bacterialwilt on potato at endemic field. This research was carried out at Kejajar Village, Kejajar Subdistrict, WonosoboRegency from June up to August 2013. The antagonists used were isolated from potato field and had been testedin the screen house and the limited field, i.e., Bacillus sp. B2 and B4, and Pseudomonas sp. P19 and P20. Basedon the research result, the antagonists could not control leaf blight and bacterial wilt, and could not increasegrowth and yield of potato. However, the antagonists could induce tannin content of the crop.Key words: antagonistic microbes, leaf blight, bacterial wilt, potato.
PEMANFAATAN BOKHASI MAHKOTA DEWA UNTUK PENGENDALIAN PENYAKIT LAYU FUSARIUM PADA CABAI MERAH Darini Sri Utami; Endang Mugiastuti
Agrin Vol 14, No 1 (2010): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2010.14.1.95

Abstract

Penelitian yang bertujuan untuk mengetahui pengaruh bokhasi dari mahkota dewa dalam pengendalianpenyakit layu Fusarium dan pengaruhnya terhadap hasil cabai merah. Penelitian menggunakan Rancangan AcakKelompok dengan 6 perlakuan, masing-masing diulang sebanyak 4 kali. Perlakuan yang dicoba adalah Fusariumoxysporum Schlecht konsentrasi 2 x 10 6 konidium per ml, tanpa mahkota dewa, 50 ml per tanaman. F.oxysporum dan air rebusan biji-cangkang mahkota dewa 40 g /100 ml air 50 ml/tanaman, F. oxysporum danbokhasi dari buah mahkota dewa yang berturut-turut ditambah laru, 200 g / tanaman, ditambah EM-4 sebanyak100 ml/tanaman, ditambah superdegra 200g /tanaman, F. oxysporum dan buah mahkota dewa, 2 buah/tanaman.Hasil penelitian menunjukkan bahwa perlakuan air rebusan biji-cangkang mahkota dewa dan bokhasi darimahkota dewa yang berturut-turut ditambah dengan laru, EM-4, superdegra dan perlakuan buah mahkota dewaberturut-turut tdk dapat menunda masa inkubasi, menekan populasi konidium dalam tanah, meningkatkan bobotbasah dan kering tanaman. Akan tetapi, air rebusan biji-cangkang mahkota dewa berpotensi menurunkanintensitas penyakit sebesar 22,03 %. Semua perlakuan tidak dapat meningkatkan hasil total cabai merah secaranyata, tetapi bokhasi dari mahkota dewa yang ditambah EM-4 atau air rebusan biji-cangkang mahkota dewaberpotensi meningkatkan hasil cabai merah masing-masing sebesar 73,18 % atau 64,32 %.Kata kunci: bokhasi mahkota dewa, pengendalian layu fusarium, cabai merah ABSTRACTThis research aimed at knowing the effect of the mahkota dewa bokhasi for controlling the disease andthe effect of the mahkota dewa bokhasi on the red chili yield. Randomized Block Design was used with sixtreatments and four replicates. The treatments tested were Fusarium oxysporum Schlecht without the mahkotadewa, F. oxysporum with the mahkota dewa seed and shell boiled water of 40 g/100 ml for 50 ml crop-1 ,F.oxysporum and the mahkota dewa fruits bokhasi added with decomposer agent (laru) of 200 g crop-1,F.oxysporum and the mahkota dewa fruits bokhasi added with EM-4 of 100 ml crop-1 , F. oxysporum and themahkota dewa fruits bokhasi added with superdegra of 200 g crop-1 , F. oxysporum and mahkota dewa fruits of 2fruits crop-1 .Result of the research showed that the treatments of the seed and shell boiled water or bokhasiadded with laru, EM-4, superdegra, and the fruis could not postpone incubation period, suppress the conidialpopulation in soil, and not increase the root weight. The mahkota dewa seed and shell boiled water treatment,however, had potency to decrease the disease intensity for 22,03%. All treatments could not increase the redchili yield, but the bokhasi made from the mahkota dewa fruits added with EM-4 and the mahkota dewa seed andshell boiled water had potency to increase the red chili yield for 74.18% and 64.32%, respectively.Key words: bokhasi mahkota dewa, fusarium wilt control, red chili.
PENGOMPOSAN LIMBAH SAYUR DENGAN EMPAT ISOLAT Trichoderma harzianum DAN PENGARUHNYA TERHADAP PERTUMBUHAN TANAMAN MENTIMUN IN PLANTA Fida Suci Ersapoetri; Loekas Soesanto; Endang Mugiastuti; Ruth Feti Rahayuniati; Abdul Manan; Slamet Rohadi
Agrin Vol 24, No 2 (2020): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2020.24.2.536

Abstract

Penelitian bertujuan mengetahui kemampuan empat isolat Trichoderma harzianum dalam mengomposkan limbah sayur, isolat T. harzianum terbaik pada pengomposan limbah sayur, dan isolat T. harzianum terhadap pertumbuhan tanaman mentimun in planta. Penelitian dilaksanakan di rumah kaca dan Laboratorium Perlindungan Tanaman, Fakultas Pertanian, Universitas Jenderal Soedirman, Purwokerto selama lima bulan. Rancangan Acak Kelompok digunakan dengan empat ulangan dan kombinasi antara empat isolat T. harzianum (T10, T213, T14, dan T15) dengan dua limbah sayur (kubis dan tomat). Variabel yang diamati adalah panjang tanaman, bobot tanaman segar, bobot tanaman kering, panjang akar, jumlah daun, pH akhir kompos, C/N ratio kompos, kepadatan akhir T. harzianum, kegigasan T. harzianum, dan analisis jaringan secara kualitatif. Hasil penelitian menunjukkan bahwa keempat isolat T. harzainum efektif dan cepat dalam mengkomposkan limbah tomat dan kubis. Isolat T. harzianum yang paling baik pada pengomposan adalah T10 dan T213. Aplikasi kompos limbah mampu meningkatkan pertumbuhan tanaman mentimun. Isolat terbaik adalah T. harzianum T16 pada kompos tomat dan T10 pada kompos kubis dalam meningkatkan pertumbuhan tanaman mentimun pada panjang tanaman, panjang akar, bobot tanaman segar, dan bobot tanaman kering dengan peningkatan masing-masing 66,61 dan 52,17%, 61,01 dan 46,55%, 76,41 dan 59,77%, serta 77,99 dan 52,03%.Kata kunci: limbah sayur, mentimun, pengomposan, Trichoderma harzianum.