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All Journal IPTEK The Journal for Technology and Science Prosiding Seminar Biologi Biota JURPIKAT (Jurnal Pengabdian Kepada Masyarakat) Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Sewagati
Awik Puji Dyah Nurhayati
Institut Teknologi Sepuluh Nopember
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Education Engineering Environmental Science Law, Crime, Criminology & Criminal Justice Public Health Social Sciences

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Journal : IPTEK The Journal for Technology and Science

 1 
The Anticancer Activity of Marine Sponge Cinachyrella sp. (Family Tetillidae) Awik Puji Dyah Nurhayati; Rarastoeti Pratiwi; Subagus Wahyuono; Istriyati Istriyati; Nichole J de Voogt
IPTEK The Journal for Technology and Science Vol 25, No 3 (2014)
Publisher : IPTEK, LPPM, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j20882033.v25i3.530

Abstract

Cinachyrella sp. marine sponge produce many kinds of secondary metabolites. The purpose of this study was to examine the cytotoxic and anti-proliferative activity of marine sponge Cinachyrella sp. The sponge was extracted with 96 % ethanol. Ethanol extract cytotoxicity assay were performed with MTT method (Microculture Tetrazolium) against to cell lines of HeLa, T47D, WiDr and Vero. The results show that of the ethanol extract were only toxic to cell lines of HeLa IC50 897.809 μg/mL but did not toxic to cell lines of T47D, WiDr and normal cell lines of Vero. Fractionation of the ethanol extract was conducted by a Vacuum Column Chromatography (VCC) and have 4 fraction that were F1, F2, F3 and F4. Cytotoxicity and cell proliferation inhibitory were tested of fraction F1, F2, F3 and F4 against cell lines of T47D. The values IC50 of F1; F2; F3 and F4 against cell lines of T47D were 82.744; 163.679; 66.522 and 333.026 μg/mL and fraction F3 concentration 31.5 µg/mL inhibits cell proliferation cell lines of T47D at 48 hours of incubation
Histology of Mice Skin Tissue Based on in Vivo Evaluation of the Anticancer Extracts of Marine Sponge Aaptos Suberitoides Awik Puji Dyah Nurhayati; Dewi Hidayati; Rarastoeti Prastiwi; Sitarina Widyarini; Sukardiman Sukardiman; Wijayanti Pujitono
IPTEK The Journal for Technology and Science Vol 22, No 1 (2011)
Publisher : IPTEK, LPPM, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j20882033.v22i1.88

Abstract

The sponge Aaptos suberitoides can produce high secondary metabolite with some farmocological activities as antimicrobial, antiviral, antiinflamatory, and anticancer agents. The purpose of this research is to find out correlations between activities of the etanol extracts of marine sponges Aaptos suberitoides on the cancer growth of subcutaneous mice (Mus musculus) injected by Benzo(a)piren. For the purpose the mice were divided into six groups, i.e. I, II, III, IV, V, and VI. Each group was treated with carcinogenic inductions by intravenously injecting the Benzo(a)piren concentration of 0.3g/0.2 ml oleum olivarum. After the cancer was appeared at the fifteenth day, the mice were treated by the anticancer extracts of marine sponges Aaptos suberitoides with concentrations of 500 mg/kg BB (Group IV), 1000 mg/kg BB (Group IV), and 1500 mg/kg BB (Group IV). The treatments were orally done each day for two weeks. At the twentieth week, subcutaneous cancer tissues were taken to make histological preparates using a parafin method. Result of the histological observation indicates that cancer in the mice was fibrosarcoma characterized by the thickening dermis layers, necrosis, mitosis, and nuclear polymorphsm. Necrosis, mitosis, and nuclear polymorphism occurred in Groups II, IV, V, and VI, and did not in Groups I and II. Presentation of necrosis was 20-60%, mitosis was in 3-4 cells, and nuclear polymorphism was 100%. Result of the statistical analyses by using the Kruskal-Wallis method and the Pair Comparison test indicates that the anticancer extracts of marine sponges with the concentrations of 500 mg/kg BB, 1000 mg/kg BB, and 1500 mg/kg BB had no activity inducing mice skin cancer.
The Anticancer Activity of the Marine Sponge Aaptos suberitoides to Protein Profile of Fibrosarcoma Mice (Mus musculus) Noor Nailis Sa'adah; Awik Puji Dyah Nurhayati; Maya Shovitri
IPTEK The Journal for Technology and Science Vol 27, No 3 (2016)
Publisher : IPTEK, LPPM, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (443.527 KB) | DOI: 10.12962/j20882033.v27i3.1183

Abstract

The study was aimed to evaluating anticancer activity of A. suberitoides on protein profile of blood plasma of fibrosarcoma mice (M. musculus). Mice were induced by Benzo(a)pyrene on the subcutaneous tissue intravenously with a concentration of 0,3 gram/0,2 oleum olivarum/day for 2 weeks. Mice were treated with ethanolic extract of A. suberitoides every day for two weeks. There were six groups. The first group (I) was control. Group II, mice were induced by CMCNa, Group III mice were treated by Cyclophosphamide. Group IV, V and VI mice were treated by ethanolic extract of A. suberitoides with 500, 1000 and 1500 mg/weight. Mice blood were taken at week fifteen. Protein profile of mice was measured by electrophoresis SDS-PAGE. The protein profile indicated that the mice were induced benzo(a)pyrene (Group II, III, IV, V and VI) have a new protein band (41 kDa). The protein band not presented in control (Group I). The protein band ( 7 kDa) was in Groups I and II, but it not presented in Groups III, IV, V and VI. The most interesting protein band was 115 kDa, which presented only in Groups II, III and IV. The research showed that the sponge extract with concentration of 1000 and 1500 mg/weight repressed the expression of protein 115 kDa
Co-Authors Adik Mayang Indiani Adik Mayang Indiani Afrilya Dina Salsabila Agus Wahyudi Agus Wahyudi Alfani Raziful Dwi Ersandy Alifia Cahyaning Putri Anak Agung Gede Sugianthara Annisya Fitri Rachmada Ary Bachtiar Krishna Putra Aulia Istighfarotunnisa Bagastyo, Arseto Yekti Danang Samudra Devi Pramesti Wulandari Dewi Hidayati Didit Prasetyo Dini Adni Navastara, Dini Adni Dini Ermavitalini Dini Ermavitalini Edwin Setiawan Elshinta Riantica Fitri Lianingsih Geoloveriana Esasi IDAA Warmadewanthi Istriyati Istriyati Kamila Islamiati Kharisma Armida Ulayya Kuswytasari, Nengah Dwianita Lissa Rosdiana Lissa Rosdiana Maitsa Adzro Fania Maya Shovitri Nadila Wulan Cahyani Natanael Hari Wijaya Ni Wayan Ni Wayan Nichole J de Voogt Noor Nailis Sa'adah Noor Nailis Sa'adah Noor Nailis Saadah Noor Nailis Saa’dah Noor Nailis Sa’adah Nova Maulidina Ashari Nova Maulidina Ashuri Nova Maulidina Ashuri Nurlita Abdulgani Nurul Jadid Rarastoeti Prastiwi Rarastoeti Pratiwi Ratna Rintaningrum Rony Irawanto Saptarini, Dian Sari, Noorkomala Shabrina Syifa Ghaissani Shabrina Syifa Ghaissani Subagus Wahyuono Sukardiman Triono Bagus Saputro Triono Bagus Saputro, Triono Bagus Welly Herumurti Wijayanti Pujitono Zufar Azzaria Azmi