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All Journal Jurnal Medik Veteriner Journal of Parasite Science

Sunarso, Agus

Department Of Veterinary Parasitology, Faculty Of Veterinary Medicine, Universitas Airlangga, Surabaya

Author-ID : 3558465

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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Prevalensi Penyakit Surra pada Sapi Potong di Kecamatan Cluring Banyuwangi Nur Sa’adah Sulaeman; Agus Sunarso; Bodhi Agustono; Poedji Hastutiek; Amung Logam Saputro; Aditya Yudhana
Jurnal Medik Veteriner Vol. 2 No. 1 (2019): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Penelitian ini bertujuan untuk menyajikan data dan informasi tentang prevalensi penyakit Surra di Kecamatan Cluring Kabupaten Banyuwangi. Penyakit Surra adalah penyakit pada ternak dan hewan liar yang disebabkan oleh Trypanosoma evansi. Jenis pemeriksaan yang dilakukan dalam penelitian ini adalah pemeriksaan parasitologis dengan ulas darah melalui pewarnaan giemsa dan metode Microhematocrit Centrifugation Technique (MHCT). Deteksi T. evansi dinyatakan positif melalui hasil pengamatan ulas darah jika nampak struktur tripomastigote dan undulating membran pada bagian posterior, sedangkan dengan uji MHCT ditunjukkan dengan adanya pergerakan dari T. evansi karena memiliki flagella bebas panjang. Hasil pemeriksaan dari 64 sampel darah sapi potong diperoleh hasil negatif. Angka prevalensi di lokasi penelitian adalah 0%. Faktor pemicu tidak timbulnya penyakit Surra pada ternak sapi potong dilihat dari keberadaan vektor, manajemen pemeliharaan, fase infeksi T. evansi dalam tubuh host, kondisi tubuh dari host, dan waktu viabilitas T. evansi dalam sampel darah.

Prevalensi Ektoparasit pada Kambing Kacang di Kecamatan Prambon Kabupaten Nganjuk Nining Sari Virgandina Vinola; Agus Sunarso; Nenny Harijani; Endang Suprihati; Poedji Hastutiek; Mufasirin Mufasirin
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this research are to identify and prevalence of ectoparasite infestation in bean goats on the sub-district of Prambon, district of Nganjuk. The research was conducted from September until December 2019 with 115 samples of bean goats. The samples were identified in the Entomology and Protozoology Laboratory of Parasitology Department of the Veterinary Faculty of Airlangga University. This research use non-experimental method with survey study. This research found several kind of extoparasites that infestation bean goats, as follows: Bovicola caprae 24.3% the most infestation in dorsal region, Linognatus africanus 31.2% the most infestation in head-neck and dorsal region, Ctenocepallides felis 4.3%. Mix infestation found B. Caprae and L. Africanus 20.8%, B. Caprae and C. Felis 1%, B. Caprae, L. Africanus and C. Felis 1%. This ectoparasite usually found infestatio in head-neck, ventral, dorsal, inguinal, and ectremites. The prevalence of ectoparasites is 82.6% area at sub-district of Prambon, district of Nganjuk. The data analyzed using Chi-square test and Correspondence test. Statistically is no significant association (p>0.05) between the prevalence of ectoparasites that infested bean goats with types of stage and non-stage eclosure. This caused due to environmental conditions are dirty and many other livestock around the cage.

Infestation Pattern of Lice In Laying Ducks In Village of Kramat District of Bangkalan Region of Bangkalan Kiki Amalia Rama; Poedji Hastutiek; Oky Setyo Widodo; Endang Suprihati; Agus Sunarso; Soeharsono Soeharsono
Journal of Parasite Science (JoPS) Vol. 1 No. 2 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this research is to identify lice and to know the pattern of infestation of lice that infest laying ducks in Village of Kramat, District of Bangkalan, Region of Bangkalan. Forty samples of laying ducks were taken in Kramat Village and identified in Entomology and Protozoology Laboratory of Parasitology Department of Veterinary Faculty of Airlangga University, the research was conducted from July to September 2017. This study used an explorative observational research design. Lice taken from a sample of laying ducks are fed into an ointment pot containing 70% alcohol to preserve lice and labeled in accordance with the infected duck's limb region. Identification of lice using Permanent mounting method without coloration. The results of identification, the lice that infest laying ducks consists of three types of Anaticola crassicornis 60%, Menacanthus stramineus 25% and Lipeurus caponis 17.5%. The results of infestation pattern, infected body's region is head-neck, wings and back. The wings infested two types of lice were A. crassicornis and L. caponis, head-neck infested M. stramineus and in the infected back region of A. crassicornis.

The Anthelmintic Activity of Ethanol Extract of Basil Leaves (Ocimum americanum Linn.) Againt Haemonchus contortus Death in Vitro Rari Noviana; Chairul Anwar Nidom; Agus Sunarso; Setiawan Koesdarto; Sri Mumpuni Sosiawati; Iwan Sahrial Hamid
Journal of Parasite Science (JoPS) Vol. 1 No. 2 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

Tujuan dari penelitian ini adalah untuk mengetahui daya anthelmintik dari ekstrak etanol daun kemangi (Ocimum americanum Linn.) terhadap mortalitas cacing Haemonchus contortus secara in vitro. Sediaan ekstrak dibuat dengan konsentrasi (berat/volume) 2,5%, 5%, 7,5%, dan 10%. Untuk kontrol positif digunakan PBS dan DMSO sedangkan untuk kontrol negatif digunakan Albendazole 10 mg/ml. Sebanyak 10 ekor cacing Haemonchus contortus betina direndam dengan berbagai perlakuan dalam cawan petri. Masing-masing perlakuan diulang sebanyak 4 kali. Pengamatan dilakukan setiap 1 jam sekali selama 6 jam untuk melihat apakah cacing masih hidup atau sudah mati. Hasil dari penelitian ini menunjukkan bahwa ekstrak daun kemangi memiliki efek anthelmintik terhadap cacing Haemonchus contortus secara in vitro. Makin tinggi konsentrasi ekstrak, maka daya anthelmintiknya juga semakin tinggi. Pada ekstrak konsentrasi 10% memiliki kemampuan anthelmintik yang hampir sama dengan Albendazole.

The Effect of Calcium Hydroxideon Mortality of Ascaridia galli In Vitro Inggarsetya Syah Audini; Setiawan Koesdarto; Ira Sari Yudaniayanti; Setiawati Sigit; Sri Mumpuni Sosiawati; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Ascaridiasis can be happened in either broiler or hens that can caused decrease of meat and egg productions. Ascaridiasis is a parasite that is most often found in poultry and cause high economic losses. This research was conducted to determine the anthelmintic effect of calcium hydroxide [Ca(OH)₂] on mortality of Ascaridia galli in vitro. In this research used Ascaridia galli with length 7-11 cm without differentiating their sex. The concentrations of calcium hydroxide [Ca(OH)₂] for immersion the Ascaridia galli were 0,01%, 0,02%, 0,025% and 0,03% as treatment group, respectively. The negative control used aquadest. Observation death A.galli worm and analysis of the data at the 2 hour, 4 hour, 6 hour, 8 hour, 10 hour and 12 hour. The result showed that the increase of concentration increased mortality of Ascaridia galli. Percentage of death worm A.galli at the 12th hour on concentration ,01%, 0,02%, 0,025% and 0,03% was 20%, 55%, 75% and 100%. ANOVA test showed significant difference between control and treatment group (p<0.05). Duncan multiple range test was seen the best treatment was the biggest mortality in concentration of 0,03%.

Prevalence and Saverity Level of Scabies (Sarcoptes scabiei) on Rabbits in Sajen Village, Pacet SUB-District, Mojokerto Regency Taufik Tri Laksono; Gandul Atik Yuliani; Agus Sunarso; Nunuk Dyah Retno Lastuti; Lucia Tri Suwanti
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Penelitian ini bertujuan untuk mengetahui prevalensi dan tingkat keparahan scabies (Sarcoptes scabiei) pada ternak kelinci di Desa Sajen, Kecamatan Pacet, Kabupaten Mojokereto. Sarcoptes scabiei var. cuniculi merupakan tungau dengan predileksi di stratum korneum dari kulit dan penyebab scabies. Gejala klinis ditandai dengan alopecia and krusta pada kulit. Penelitian ini menggunakan metode penelitian eksploratif labolatorik dengan pendekatan cross sectional. Sampel yang digunakan adalah kelinci pada satu peternakan yang memiliki gejala klinis scabies dan hasil dari kerokan kulit pada pemeriksaan laboratoris secara natif. Kelinci yang positif scabies kemudian dilakukan skoring berdasarkan tingkat keparahan pada regio tubuh dan gejala klinisnya. Hasil penelitian menunjukkan bahwa dari 110 ekor kelinci dalam populasi, terdapat 19 ekor yang terinfeksi scabies dan terdapat 2 tingkat keparahan scabies pada kelinci yaitu tingkat keparahan ringan dan sedang yang terbagi atas 2 regio tubuh. Tingkat keparahan ringan menunjukkan gejala klinis alopecia dan krusta pada regio wajah sedangkan tingkat keparahan sedang pada regio wajah dan kaki. Kesimpulan pada penelitian ini adalah angka prevalensi scabies sebesar 17,27% dan tingkat keparahan ringan sebesar 26,32% serta tingkat keparahan sedang sebesar 73,68.

Investation of Ectoparasites In Chickens In Village of Kramat District of Bangkalan Regency of Bangkalan Fania Selfiannisa; Suherni Susilowati; Poedji Hastutiek; Lucia Tri Suwanti; Kusnoto Kusnoto; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 2 No. 2 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

A study on the infestation of ectoparasites in chicken’s raised in Village of Kramat, District of Bangkalan, Regency of Bangkalan was conducted between July to October 2017. A total of 60 chickens were examined based on their sex and region of the chicken’s body. Identification was carried out on ectoparasites found identified using permanent mounting methods and scraping. The types of ectoparasites were M. gallinae 77%, L. caponis 52%, M. stramineus 15%, R. sanguineus 3%, G. gallinae 2%, G. gigas 2%, and D. gallinae 2%. Data were analyzed using Chi Square and Correspondence. The results of infestation was higher in males than females, although the difference was not statistically significant (P>0.05). Results of analysis of the pattern of investation of ectoparasites in the region that includes in males and females body are M. gallinae in the region of the back. L. caponis in the region of the wings. M. stramineus. R. sanguineus in the region of the head-neck, G. gigas in the region of belly, G. gallinae in the region of the leg just found in the males body. D. gallinae in the region of the leg just found in the females body.

Prevalence of Protozoa in Gastrointestinal Tract of Pigeons (Columba livia) Maintenance Ekstensif and Intensif in Surabaya Felita Widyaningsih; Muchammad Yunus; Rimayanti Rimayanti; Setiawan Koesdarto; Lucia Tri Suwanti; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 2 No. 2 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

This research was conducted to determine the prevalence of major gastrointestinal protozoal infections, the kinds of protozoa that infects and maintenance systems influence on pigeons in Surabaya. Pigeon taken from five regions of Surabaya namely North Surabaya, East Surabaya, West Surabaya, South Surabaya and Surabaya Center. Each region is taken as many as 20 pigeons consisted of 10 pigeons with extensive maintenance system and 10 pigeons with intensif maintenance system. So that the total overall sample is 100 pigeons. Laboratory examination (microscopic) was conducted in November 2017 until January 2017. Examination of the upper gastrointestinal tract using throat swabs and stool samples were examined with native methods, sedimentation, and flotation. Identification using a microscope using 40x objective scale and 10x oculer scale to determine gastrointestinal protozoal that was found. Data was analyzed using Chi Square Test statistical analysis. Results showed 78% of the positive samples gastrointestinal protozoa infections in pigeons in the city of Surabaya. Results of Chi Square Test statistical analysis showed a p value of 0.030 (p<0.05) which means significantly different. Types of protozoa found that Trichomonas gallinae (49%) and Eimeria sp. (61%). The prevalence of gastrointestinal protozoal pigeons on extensive maintenance sistem was higher (44%) compared to the intensive caresis system(34%).

Prevalence of Gastrointestinal Parasite on Cats in Shelter East Surabaya Akbar Wijaya Putra Purnama; Lucia Tri Suwanti; Hani Plumeriastuti; Endang Suprihati; Kusnoto Kusnoto; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this study was to determine the prevalence of gastrointesatinal parasites in cats at Surabaya shelter. Eighty two samples of cat feces used in this study were taken from Griya satwa shelter and Kiro cat shelter. Feces samples were examined using the native, sedimentation and floating methods. Feces samples gastrointestinal parasite were identified by using a microscope with 400x and 1000x magnifications. Faces samples that are diagnosed positively if there is at least one of the three methods. Feces samples has observed, obtained prevalence rate of 43.9% or 36 positive samples from eighty two samples. The results of chi square analysis showed that p > 0.05, which showed that the parasites taken in the two shelters had clear differences in the prevalence of the gastrointestinal tract parasites. The parasites observed were Isospora sp., Ancylostoma sp., Toxocara cati and Toxocara leonina.

The Potential of Anting – Anting (Acalypha indica L.) Leaf Extract as Anti-Scabies to Sarcoptes scabiei var. Cuniculi in vitro Luluk Tri Astuti; Rahmi Sugihartuti; Lianny Nagoi; Nunuk Dyah Retno Lastuti; Dewa Ketut Meles; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The Aim of research to determine the potential of anting-anting (Acalypha indica L.) leaf extract as anti-scabies to Sarcoptes scabiei var. Cuniculi in vitro by observing the mortality rates and lethal concentration of Sarcoptes scabiei. There were 5 treatments namely DMSO 0.5% (PN). Amitraz (P0), anting-anting (Acalypha indica L.) leaf extract concentratrion of 6,25% (P1), 12,5% (P2) and 25% (P3). Observations were carried out for 8 hours with stereomicroscope. The data was analyzed using rpobit analysis with SPSS 20.0 software. The results showed that the fastest mortality rate of S. Scabiei was group p3 at 4.6 mites/hour., then P0, P2, P1 and PN respectively 3.5, 3.1, 1.9, and 1.3 mites/ hour. The smallest concentration of anting-anting (Acalypha indica L.) leaf extract was not significantly different compared to amitraz for killing of 50% and 90% S. Scabiei was 12.5% with lethal time (LT50) 1.82 hours and (LT90) 3.69 hours. In conclusion, anting-anting (Acalypha indica L.) leaf extract has mortality rate against S. Scabiei var. Cuniculi of 3.1 mites/hour, lethal concentration of 12.5% and potential as anti-scabies in vitro.

Co-Authors Aditya Yudhana Akbar Wijaya Putra Purnama Amung Logam Saputro Andhika Yudhantama Subroto Bodhi Agustono Boedi Setiawan Chairul Anwar Nidom Dewa Ketut Meles E Djoko Poetranto Endang Suprihati Fania Selfiannisa Felita Widyaningsih Fifi Anik Suroiyah Gandul Atik Yuliani Hani Plumeriastuti Herry Agoes Hermadi I Komang Wiarsa Sardjana Inggarsetya Syah Audini Ira Sari Yudaniayanti Iwan Sahrial Hamid Kartika Aditiya Amelia Khoirul Arifin Kiki Amalia Rama Kusnoto Kusnoto Lianny Nagoi Lucia Tri Suwanti, Lucia Tri Luluk Tri Astuti Mohammad Sukmanadi Muchammad Yunus Mufasirin Muhammad Thohawi Elziyad Purnama Nadya Amanda Chaerunissa Nenny Harijani Nining Sari Virgandina Vinola Novia Intan Kurnia Nunuk Dyah Retno Lastuti Nur Sa’adah Sulaeman Oky Setyo Widodo Poedji Hastutiek Primarizky, Hardany Rahaju Ernawati Rahmi Sugihartuti Rari Noviana Ratih Novita Praja Rimayanti Rimayanti Setiawan Koesdarto Setiawati Sigit Silvia Martha Kristina Soeharsono Soeharsono Sri Mumpuni Sosiawati Suherni Susilowati Taufik Tri Laksono Vivi Oktaviana

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