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All Journal Jurnal Veteriner Majalah Obstetri dan Ginekologi Jurnal Medik Veteriner Veterinary Biomedical and Clinical Journal Journal of Parasite Science

Boedi Setiawan

Department Of Veterinary Clinic, Faculty Of Veterinary Medicine, Universitas Airlangga, Surabaya

Author-ID : 3558547

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

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Terapi Fotodinamik Mempercepat Kesembuhan Luka Insisi pada Kulit Tikus Putih (Rattus novergicus) yang Diinfeksi Bakteri Methicilin-Resistant Staphylococcus aureus Yonatan Dimascahyo Budianto; Lucia Tri Suwanti; Wiwik Misaco Yuniarti; Hani Plumeriastuti; Wiwiek Tyasningsih; Boedi Setiawan
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.591 KB)

The aims of this research was to determine the effect of photodynamic therapy (PDT) on the healing of incision wounds in rat skin (Rattus norvegicus) that infected with MRSA bacteria by evaluating and give a score (0 – 4) at wound healing factors such as epithelialization, angiogenesis, inflammatory cells, fibroblast cells and collagen density. This study used 25 white rats divided randomly into 5 groups with 5 replications, namely P0 = MRSA infection wound + Povidone Iodine, P1 = MRSA infection wound + Silver Sulfadiazine, P2 = MRSA infection wound + 5-minute PDT irradiation, P3 = MRSA infection wound + 10- minute PDT irradiation and P4 = MRSA infection wound + 15-minute PDT irradiation. Histopathological observations and scoring showed epithelialization and collagen density in groups P2, P3 and P4 increased significantly compared to groups P0 and P1 (p<0.05). The histopathological observations and scoring of angiogenesis, inflammatory cells, fibroblast cells of P0 and P1 groups had a higher score than the treatment group with photodynamic therapy P2, P3 and P4 (p<0.05). This condition showed that in the P0 and P1 groups are still in the inflammatory phase, while in the P2, P3 and P4 groups have entered the remodeling phase which is characterized by high epithelialization scores and collagen density. Giving photodynamic therapy to wounds infected with MRSA can accelerate the wound healing process.

New vesicovaginal fistula formation in a rabbit model Eighty M Kurniawati; Tri HS Hadi; Nur AR Widiatmoko; Widjiati Widjiati; Fedik A Rantam; Budi Santoso; Budi I Santoso; Hari Paraton; Gatut Hardianto; Azami D Azinar; Boedi Setiawan
Majalah Obstetri dan Ginekologi Vol. 27 No. 3 (2019): December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mog.V27I32019.114-118

Objective: To create an animal model of vesicovaginal fistula as a modality to develop therapy.Material and Methods: This study was an preliminary experimental study to create new fistula vesicovaginal formation in a rabbit model. Six female New Zealand white rabbits of Stem Cell Research and Development Center of Universitas Airlangga were used in this study and were divided into 3 groups in which there were 2 rabbits in each group. Vesicovaginal fistula formation was performed through laparotomy surgery. After the abdomen is opened, the Nasogastric tube is inserted vaginally into the anterior vaginal wall. The incision is made on the anterior vaginal wall and the posterior wall of the bladder. The Nasogastric tube that comes out of the vagina is inserted into the bladder incision to maintain the formation of vesicovaginal fistula. Both mucosal walls are sutured and tested to determine if there is any leak. Evaluation the model of vesicovaginal fistula was carried out 3 weeks postoperatively according the group.Result: Four out of six rabbits have formed vesicovaginal fistulas, which are from groups two and three. There are no complications during the treatment period until terminationConclusion: Rabbits have the potential to become experimental animals for the vesicovaginal fistula model. The best time to release a nasogastric tube to maintain a fistula is 2 weeks.

Prevalence and Intensity of Nematode Infection on The Crossbreed Horse in Detasemen Kaveleri Berkuda Parongpong Bandung West Java Sesa Puput Febriyanti; Lucia Tri Suwanti; Eka Pramyrtha Hestinah; Setiawan Koesdarto; Boedi Setiawan; Kusnoto Kusnoto
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

This survey study was aimed to determine the prevalance of nematodosis and intensity on the Crossbreed Horse in Detasemen Kaveleri Berkuda Parongpong Bandung West Java. The benefits of this research are to contribute giving additional information about Prevalence, Intensity, and Relation among prevalance with age, sex, and stable. Fecal samples were tested by native, sedimentation, and floatation methods. The examination was found some kind of worm eggs, which are: Trychonema sp., Strongylus spp., Dictyocaulus arnfieldi (lung worm). The result showed that the prevalence of nematodosis was 12.03%. in the calculation of worm eggs per gram feces of Crossbreed Horse in Detasemen Kaveleri Berkuda that have relatively moderate infection were 1123EPG and result analyze with ANOVA showed no real different with function of horse. The result of statistical analysis using Chi-Square test showed no significant differences in prevalence with age and sex (p>0.05). the result of statistical using Chi-Square test showed significant differences in prevalence with stable in Detasemen Kaveleri Berkuda (p<0.05).

The Effect of Folic Acid as Supportive Therapy of Spiramycine on Weight of Foetus to Toxoplasma gondii – Infected Pregnant Mice (Mus musculus) Alfina Azkiana; Boedi Setiawan; Erma Safitri; Lucia Tri Suwanti; Mufasirin Mufasirin; Djoko Legowo
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

This research aimed to investigate the effect of folic acid as supportive therapy of spiramycine on weight of foetus to Toxoplasma gondii-infected pregnant mice (Mus musculus). Twenty pregnant female mice were divided into four groups as C -, C +, T1 and T2. C +, T1 and T2 were infected by Toxoplasma gondii. C – and C + administered orally 0.5 ml aquadest, T1 administered orally 130 mg/kg BW spiramycine and 0.052 µg/g BW folic acid and T2 administered orally 0.052 µg/g BW folic acid. Experimental groups received the treatments for 5 days, then animals of each groups were sacrified. Foetuses were dissected out for observation. The weight of fetuses were measured using an analytical balance. The data weight of foetuses was presented descriptively and analyzed by ANOVA test and continued by Tukey HSD. From this study, the weight of foetuses from the pregnant mice of T1 and T2 have difference compared with the controls. The result of this research is folic acid affects the weight of foetuses to Toxoplasma gondii-infected pregnant mice.

The Distribution of Goat Gastrointestinal Tractus Worm Egg at Rambon District of Nganjuk Regency Arum Puspitasari; Boedi Setiawan; Setiawan Koesdarto; Kusnoto Kusnoto; Soeharsono Soeharsono; Poedji Hastutiek
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

This study aims to determine the distribution of worm tract infection in foat digestion in Sub-district of Prambon, Nganjuk Regency. This research was conducted in Agustus 2018-Januari 2019 with 108 samples of stool examination iin laboratory of Helmintologi Airlangga University Department of Parasitology, were examinated by native, sedimentation. Floatation techniques, and distribution of worm types of feces. The results showed that 66 samples were positive infected by gastrointestinal worm, its indicated that helminthiasis was 61,11%. On examination it was found some kind of worm eggs, which are: Oesophagustomum spp., Bunostomum spp., Haemonchus spp., Trichostrongylus spp., Strongyloides spp., Trichuris spp., Chabertia spp and Moniezia benedini. The results of study showed the most of worm distribution in feces is Haemonchus spp.

Identifikasi Protozoa pada Darah dan Saluran Pencernaan Biawak Air (Varanus salvator) Azizah Bilqis Nurkarimah; Mufasirin Mufasirin; Ratna Damayanti; Lucia Tri Suwanti; Boedi Setiawan; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

The aim of this research is to identify the variaous of protozoa in the bloodanddigestive tract on water monitor lizard (Varanus salvator) was captured in Sidoarjo, East Java. This research was taken on March until June 2019. This research used a non-experimental method through an observation study. As many as 50 water monitor lizard were used this research and examined at the Laboratory of Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga.The samples of this research were made in thin blood smear using Giemsa stain, while fecal examination using native method, sucrose flotation, and modified Ziehl-Neelsen stain.The results showed that two various of protozoa found were single infection. Observations on the blood was obtained Haemogregarina sp. (14%) and observations of feces obtained Eimeria sp. (2%). The conclusion of this research indicate that type of protozoa was Haemogregarina sp. and Eimeria sp. were found on water monitor lizard(Varanus salvator).

Identifikasi dan Pola Infestasi Caplak pada Biawak Air (Varanus salvator, Byers, d. 2000) Kartika Aditiya Amelia; Rahaju Ernawati; Poedji Hastutiek; Boedi Setiawan; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

The aim of this study is to identify the type of ticks infestation pattern on water monitor lizard (Varanus salvator, Byers, D. 2000) with regard parameters water monitor lizard species, stadium and region of the water monitor lizardâ€™s body. The total samples of 250 ticks from 30 water monitor lizard (18 male lizards and 12 female lizards) were collected every day starting from noon to evening during July to August 2019 at the monitor slaughtering place in Buduran District, Sidoarjo Regency. The tick samples were identified by the permanent mounting slide method without staining and put in bottles containing 10% KOH for 1-10 hours, then each stadium used a nikon eclipse E100 microscope with a 40x magnification binocular microscope. Data was analyzed using Multiple Correspondance Analytics. The result indicate that 30 V. salvator (100%) are positive infested by tick and the samples obtained consisted of 133 Aponomma sp. and 117 Amblyomma sp. in all regions of the body.

Identification of Ectoparasites and Endoparasites on Java Langurs (Trachypithecus sp.) and Silvery Gibbons (Hylobates moloch) in The Aspinall Foundation Indonesia Program Ghifari Lutfi Fauzi; Endang Suprihati; Poedji Hastutiek; Boedi Setiawan; Retno Wulansari
Journal of Parasite Science (JoPS) Vol. 5 No. 1 (2021): Journal of Parasite Science
Publisher : Universitas Airlangga

This study aimed to determine the prevalence rate and to identify parasites (endoparasite and ectoparasite) on Java Langur and Silvery Gibbon, these primates are rehabilitated in The Aspinall Foundation Indonesia Program. This research was conducted from February until October 2020. The fecal examination methods used in this research were native method and ovatec flotation method, while the ectoparasite examination used native method, mounting method, and scraping method. Based on a total of 68 samples examined, the result showed that 10 samples were positive infected by Trichuris trichiura and 2 samples were infested by Pedicinus ancoratus. The conclusion of this study indicated that the prevalence rate of Trichuris trichiura was 21.1% and Pedicinus ancoratus was 5.3% on 19 West Java Langurs, the prevalence rate of Trichuris trichiura was 3.3% and Pedicinus ancoratus was 3.3% on 30 East Java Langurs, the prevalence rate of Trichuris trichiura was 26.3% and no infestation of Pedicinus ancoratus was found on 19 Silvery Gibbons.

Efektivitas Ekstrak Daun Afrika (Vernonia amygdalina) Secara Topikal Terhadap Kepadatan Kolagen dalam Penyembuhan Luka Insisi Pada Tikus Putih (Rattus norvegicus) Rona Taufiqul Rachmanita; Hardany Primarizky; Boedi Setiawan; Bodhi Agustono; Amung Logam Saputro
Jurnal Medik Veteriner Vol. 2 No. 1 (2019): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Tujuan dari penelitian ini adalah untuk mengetahui efektivitas pemberian ekstrak daun Afrika (Vernonia amygdalina) secara topikal terhadap kepadatan kolagen dalam penyembuhan luka insisi pada tikus putih (Rattus norvegicus). Dua puluh ekor tikus putih dibagi secara acak ke dalam lima kelompok, yaitu kontrol negatif (K-) yang diberi basis salep, kontrol positif (K+) yang diberi salep povidone iodine 10%, kelompok perlakuan (P1, P2, P3) yang diberi perlakuan dengan salep ekstrak daun Afrika 16,8%, 28%, dan 39,2%. Perlakuan diberikan secara topikal pada lokasi luka insisi sebanyak dua kali sehari selama empat belas hari. pada hari keempat belas tikus dieuthanasi dan daerah insisi dieksisi untuk preparasi histopatologi. Data yang didapatkan dianalisis menggunakan Kruskal-Wallis dengan SPSS 20. Hasil uji menunjukkan p=0,077 yang berarti tidak terdapat perbedaan yang nyata (p>0,05) antara keseluruhan perlakuan. Berdasarkan hasil tersebut rata-rata P1 menunjukkan hasil skoring kepadatan kolagen tertinggi dibandingkan perlakuan yang lain. Kesimpulan penelitian ini yaitu bahwa daun Afrika (Vernonia amygdalina) tidak efektif meningkatkan kepadatan kolagen dalam penyembuhan luka insisi pada tikus putih (Rattus norvegicus).

Pengaruh Perendaman Daging Sapi dalam Larutan Rimpang Kunyit (Curcuma domestica val.) dengan Kombinasi Konsentrasi dan Lama Waktu Penyimpanan Terhadap Total Jumlah Bakteri Tri Lestari; Boedi Setiawan; Ratih Novita Praja; Ratna Damayanti; Ragil Angga Prastiya; Prima Ayu Wibawati
Jurnal Medik Veteriner Vol. 2 No. 1 (2019): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Tujuan penelitian ini adalah untuk mengetahui pengaruh antibakteri dari larutan rimpang kunyit (Curcuma domestica Val.) terhadap total jumlah bakteri pada sapi dengan kombinasi antara konsentrasi dan lama penyimpanan. Sampel daging sapi dibagi menjadi 60 buah dan masing-masing sampel adalah 25 gram. Metode penelitian adalah rancangan acak lengkap dengan menggunakan 4 konsentrasi larutan rimpang kunyit 0 gr 100 ml-1, 20 gr 100 ml-1, 40 gr 100 ml-1, 60 gr 100 ml-1 dan disimpan dalam waktu berdurasi 0 jam, 3 jam, 6 jam. Data yang diperoleh dianalisis oleh menggunakan Anava. Hasil penelitian ini menunjukkan perbedaan yang signifikan (p<0,05) antara kontrol dan perlakuan pada total jumlah koloni bakteri. Berdasarkan hasil dapat disimpulkan bahwa larutan rimpang kunyit berpengaruh pada penurunan jumlah koloni bakteri.

Co-Authors Aditya Yudhana Alfina Azkiana Amung Logam Saputro Amung Logam Saputro Anindita Khairunnisa Arum Puspitasari Azami D Azinar Azizah Bilqis Nurkarimah Bodhi Agustono Budi I Santoso Budi Santoso Dhandy Koesoemo Wardhana Dinda Dwi Prastika Djoko Legowo Eighty M Kurniawati Eka Pramyrtha Hestinah Endang Suprihati Erma Safitri Fedik A Rantam Gatut Hardianto Ghifari Lutfi Fauzi Hani Plumeriastuti Hari Paraton Ira Sari Yudaniayanti Kartika Aditiya Amelia Kusnoto Kusnoto Lucia Tri Suwanti, Lucia Tri Mufasirin Nunuk Dyah Retno Lastuti Nur AR Widiatmoko Nusdianto Triakoso Poedji Hastutiek Prima Ayu Wibawati Primarizky, Hardany Ragil Angga Prastiya Rahaju Ernawati Rahmi Sugihartuti Rangga Yulianto Ratih Novita Praja Ratna Damayanti Retno Wulansari Rona Taufiqul Rachmanita Sesa Puput Febriyanti Setiawan Koesdarto Soeharsono Soeharsono Sunarso, Agus Tri HS Hadi Tri Lestari Tyasningsih, Wiwiek Widjiati Widjiati Wiwik Misaco Yuniarti Yonatan Dimascahyo Budianto

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