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All Journal Jurnal Veteriner Jurnal Medik Veteriner Jurnal Nutrisi Ternak Tropis (JNT) Ovozoa: Journal of Animal Reproduction Bantara Journal of Animal Science
Bodhi Agustono
Animal Husbandry, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Immunology & microbiology Veterinary

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Journal : Ovozoa: Journal of Animal Reproduction

 1 
The addition of vitamin C in tris–egg yolk extender maintained Sapera goat semen quality in 5° C storage Jolly Rifqi Pahlevy; Hermin Ratnani; Tjuk Imam Restiadi; Faisal Fikri; Amung Logam Saputro; Bodhi Agustono
Ovozoa : Journal of Animal Reproduction Vol. 11 No. 1 (2022): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i1.2022.1-8

Abstract

Goats are small ruminants that are reared by the rural community for financial income and nutrition. This study was aimed to determine the effect of vitamin C addition in tris–egg yolk extender on the lifespan of Sapera goat spermatozoa stored in 5o C. Semen was collected from 1.5 years old Sapera male goat. The ejaculates were diluted in Tris-egg yolk (T-EY) extender added with 0, 0.2, 0.3, and 0.4 g vitamin C/mL extender for T0, T1, T2, and T3 groups, respectively. The spermatozoa motility, viability, and morphological abnormality were assessed along with storage at 5° C. The result showed that spermatozoa motility was the highest (p <0.05) in T1 compared to other groups and qualified for AI use for up to 72 hours. In 24 hours storage, the spermatozoa viability was not significantly different (p >0.05) among the groups. The spermatozoa viability in the T1 group was the highest (p <0.05) among the groups along 48-96 hours of storage. The spermatozoa morphological abnormalities of the T1 group was the lowest (p <0.05) compared to other treatment groups in the range of 24-72 hour storage. In the control group (T0), the less than 5% spermatozoa morphological abnormalities (qualified for artificial insemination) were only in the 24 hours storage, while those of the T1 group were up to 72 hours. It could be concluded that the addition of 0.2 g vitamin C/100 mL T-EY extender maintained the quality of Sapera goat semen for 72 hours at 5°C.
Pre-freezing at 10 cm above liquid nitrogen surface for eight minutes resulted the best Sapera goat semen quality Zehansyah Algifari Pasha; Bodhi Agustono; Amung Logam Saputro
Ovozoa : Journal of Animal Reproduction Vol. 11 No. 1 (2022): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i1.2022.27-33

Abstract

Sapera goat is a dairy goat resulting from a cross between a Saanen buck and an Ettawah cross doe. These small ruminants are reared by rural households for poverty reduction and undernutrition prevention. Breeding of Sapera goat through artificial insemination (AI) technique was expected to accelerate the increase in population. Unfortunately, the frozen semen of Sapera buck is not available yet. This study aims to determine the effect of the distance between semen straw and liquid nitrogen surface during pre-freezing process on the quality of frozen semen of Sapera goats. Semen was obtained from 1.5 years old Sapera buck. The ejaculates were diluted in tris-egg yolk extender to obtain a concentration of 120 x 106 spermatozoa/mL, then equilibrated at 5°C for one hour. The extended semen was packaged in 0.5 mL French straws (60 x 106 spermatozoa/straw). The filled straws were pre-frozen in the cold handling cabinet (Minitube, Germany) held at 10, 11, 12, 13, 14, and 15 cm above liquid nitrogen respectively for T1, T2, T3, T4, T5, and T6 groups, each with four replicates. After pre-freezing for eight minutes the straws were immediately plunged, and stored in liquid nitrogen (-196°C) for two days, followed by quality assessment. The best post-thawed motility (41.75 ± 2.06 %), viability (49.00 ± 0.82 %) and morphological abnormalities (4.75 ± 0.96 %) were obtained from pre-freezing stage with a distance of 10 cm. It could be concluded that in tris-egg yolk extender, post-thawed spermatozoa viability and motility of Sapera buck were qualified for AI when fresh semen was pre-frozen at 10 cm distance of straws from the surface of liquid nitrogen for eight minutes. 
Co-Authors Aditya Yudhana Adyayutti Wijang Saraswati Amung Logam Saputro Amung Logam Saputro Amung Logam Saputro Amung Logam Saputro Amung Logam Saputro Amung Logam Saputro Amung Logam Saputro Anwar Ma’ruf Boedi Setiawan Erma Safitri Fahlevi Lailiyah Faisal Fikri Firman Setyawan Hermin Ratnani Jolly Rifqi Pahlevy Khafsah Khafsah Maya Nurwartanti Yunita Mirni Lamid Mohammad Anam Al-Arif Muhammad Thohawi Elziyad Purnama Novi Fitria Hanifah Novia Nur Iskandari Nur Sa’adah Sulaeman Nusdianto Triakoso Poedji Hastutiek Prima Ayu Wibawati Primarizky, Hardany Pudji Srianto Ragil Angga Prastiya Ragil Angga Prastiya Ragil Angga Prastiya Rahmahani, Jola Rangga Yulianto Ratna Damayanti Restiadi, Tjuk Imam Rona Taufiqul Rachmanita Sardjito, Trilas Siti Komariyah Sri Pantja Madyawati Suherni Susilowati Sunarso, Agus Tri Wahyu Suprayogi Warsito, Sunaryo Hadi Widya Paramita Lokapirnasari Zehansyah Algifari Pasha Zeni Prastika