Article Per Year (5 Year)
p-Index From 2019 - 2024
6.172
P-Index
This Author published in this journals
All Journal ESENSI: JURNAL BISNIS DAN MANAJEMEN ETIKONOMI Islamadina : Jurnal Pemikiran Islam JURNAL ILMIAH MANAJEMEN & BISNIS EQUILIBRIUM JMM17: Jurnal Ilmu Ekonomi dan Manajemen Media Trend: Berkala Kajian Ekonomi dan Studi Pembangunan International Journal of Artificial Intelligence Research EKONOMIS : Journal of Economics and Business International Journal of Supply Chain Management KOMIK (Konferensi Nasional Teknologi Informasi dan Komputer) Ekuilibrium : Jurnal Ilmiah Bidang Ilmu Ekonomi ISOQUANT : Jurnal Ekonomi, Manajemen dan Akuntansi Jurnal Maksipreneur: Manajemen, Koperasi, dan Entrepreneurship JPM PAMBUDI Almana : Jurnal Manajemen dan Bisnis Jurnal Akuntansi dan Bisnis CAM JOURNAL: Change Agent For Management Journal Jurnal Manajerial Jurnal Riset Entrepreneurship ASSET: Jurnal Manajemen dan Bisnis Masyarakat Berdaya dan Inovasi Budimas : Jurnal Pengabdian Masyarakat ANALISIS AKTUAL Jurnal Manajemen dan Inovasi (MANOVA) Journal of Entrepreneurship & Business Jurnal Valuasi : Jurnal Ilmiah Ilmu Manajemen dan Kewirausahaan Bussman Journal : Indonesian Journal of Business and Management BISMA (Bisnis dan Manajemen) Makara Journal of Science Makara Journal of Technology JIM: Jurnal Ilmiah Mahasiswa Pendidikan Sejarah
Santoso, Adi
Universitas Muhammadiyah Ponorogo
Religion Arts Humanities Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mechanical Engineering Nursing Social Sciences Transportation Other

Published : 53 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : Makara Journal of Technology

 1 
Effect of Methanol Induction and Incubation Time on Expression of Human Erythropoietin in Methylotropic Yeast Pichia Pastoris Santoso, Adi; Herawati, Neng; Rubiana, Yana
Makara Journal of Technology Vol. 16, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Erythropoietin (EPO) is a glycoprotein hormone consists of 165 amino acids and has molecular mass of 30,400 Daltons. The large quantities of these hormone required to satisfy clinical demand are currently met by recombinant expression in mammalian cell, namely chinese hamster ovary (CHO). Pichia pastoris has become popular yeast based protein production systems to substitute mammalian expression systems. P. pastoris is capable to use methanol as sole carbon and energy source. In this study, recombinant human EPO (rhEPO) protein obtained by expressing the hEPO gene in methylotropic yeast P. pastoris, strain X33. The present work was carried out to study the optimal methanol concentration for induction and the incubation time to obtain rhEPO protein. To perform this study, the transformed P. pastoris was induced with various concentrations of methanol (0%, 0.5%, 1%, 2.5%, 5%, 10%, and 20%) and incubation times (0 hours, 24 hours, 48 hours, 72 hours, 96 hours, 120 hours, and 144 hours). The results demonstrate that the highest protein expression level occurred at concentration of 2.5% methanol induction, while the optimal incubation time was at 48 hrs.
Co-Authors Abdurohim, Dindin Aghnia Salma Salsabila Alberta Adinata Alifah Febirika Nurjanah Ananda, Yudha Rizky Bety Oktavia Rahayu Chamidah, Siti Chamidah, Siti Dimas Alfikri Pramudya Dimas Dipa Pratama Dwi Warni Wahyu Dwi Warni Wahyuningsih Edi Kurniawan Edy Kurniawan, Edy Eka Destriyanto Pristi Eka Destriyanto Pristi Eka Destriyanto Pristi Ayuningtyas Evit Harmawati Febrianti Muji Trianawati Felix Ardianto* Fery Setiawan Fery Setiawan Fery Setiawan Fery Setyawan Fery Setyawan Fitri Suratin Hadi Sumarsono Herma Rio Candra Saputra Irmanida Batubara Isna Nur Rahmawati La Ode Sugianto Laksono Trisnantoro Naning Kristiyana Naning Kristiyana Naning Kristiyana Naning Kristiyana, Naning Naning Kristyana Nayni Nayni Neng Herawati, Neng Nisa'u Lailatizzahro Nistirohah Nur Fitriani Norma Hapsary Nurdiana Nurdiana Nurul Iman Nurul Iman, Nurul Radita Noviani Ratri Widya Wulandari Regyta Aprylia Reny Kusumaningrum Revina Lindya Kusuma Riawan Riawan Rizki Gagah Saputra Ryza Wahyu Anofa Sepbrio Ian Pradana Siska Maryunitasari Sri Hartono Titi Rapini Titi Rapini, Titi Titis Purwaningrum Umi Farida Umi Farida Umi Farida Vebrian Dwisakti* Wahna Widhianingrum Wahna Widhianingrum Wahna Widhianingrum Wayan Wijaksono Windu Praditiyo Subarkah Yana Rubiana, Yana Yana Rubiyana, Yana Yuliana Mariya Ulva