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All Journal Jurnal Ilmu Pertanian Jurnal Veteriner JOURNAL OF COASTAL DEVELOPMENT Zuriat Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Biotechnology Jurnal Pertanian Agros jurnal Ilmu Pertanian (Agricultural Science) Menara Perkebunan Proceeding Biology Education Conference Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Sukarti Moeljopawiro
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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Journal : Jurnal Veteriner

 1 
Respons Imunoglobulin-G dan Imunoglobulin-M Mencit yang Diberi Ekstrak Methanol Alga Biru Hijau dan Diinfeksi Dengan Takizoit Sorta Basar Ida Simanjuntak; Sukarti Moeljopawiro; Wayan Tunas Artama; Subagus Wahyuono
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Toxoplasmosis is an infectious disease caused by Toxoplasma gondii. This disease could severelyaffect humans and animals. Up to now there has been no simple treatment to fight toxoplasmosis. Aprospective alternative treatment to overcome this problem is by increasing immunity of the body using animmunostimulant such as Spirulina platensis. The aims of this research were to observe the potency of S.platensis as an immunostimulant and to find the most potential fraction of S. Platensis that can increasethe responses of IgG and IgM antibodies againts toxoplasma. The responses of these antibodies weremeasured using ELISA method. The isolation of compounds from S. platensis using Preparative ThinLayer Chromatography (PTLC) found three fractions which were a top fraction (I), a middle fraction (II),and a lower fraction (III). Forty-eight mice used in this research were divided into four different groupswith 12 mice in each group and treated differently. The top, middle, and lower fractions of S. platensis wereadministered orally to three groups of mice respectively at dose of 3mg/ml for each mouse while the micein the fourth group were kept as untreated controls. The treatment was conducted for 14 days consecutivelyand on the next day, all mice, including the controls, were challenged with tachizoit. The effect of S.platensisfractions on the responses of IgG and IgM antibodies were then measured at various time intervals, i.e. day0 (before infection) and day 1, 2, and 3 after infection. The results showed that IgG response increased inthe day 0 (2.504 OD) and the day 3 after infection (2.608 OD) while IgM response increased in day 1 afterinfection (2.898 OD). In conclusion, S. platensis was an immunostimulant and the middle fraction (II) of S.Platensis was the most potential fraction to increase immunity againts toxoplasma .
Chitosan Mempertipis Dinding dan Memperbesar Diameter Lumen Arteri Koroner Tikus Putih yang Diberi Pakan Lemak Tinggi (CHITOSAN COULD THINEN WALL AND WIDEN LUMEN DIAMETER CORONARY ARTERY OF SPRAGUE DAWLEY RATS INDUCED BY HIGH FAT RATION) Sri Isdadiyanto; Sukarti Moeljopawiro; Nyoman Puniawati; Hastari Wuryastuty
Jurnal Veteriner Vol 14 No 3 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to analyze the influence of chitosan on coronary artery wall thicknessand lumen diameter of Sprague Dawley rats induced by high fat ration. The animals for this study weretwenty adult male rats divided into four groups, i.e. group I as the control was fed with basal rationcontaining normal fat for 3 months, group II was fed ration containing high fat for three months, group IIIwas fed ration containing high fat and given chitosan 180 mg per kg body weight per day orally in 2 mLaquadest for 3 months, group IV was fed ration containing high fat for three months and after one monthgiven chitosan 180 mg per kg body weight per day orally in 2 mL aquadest for two months. Each groupconsisted of five animals. After 90 days, the rats were necropsied and the hearts were collected tohistopathological. The difference between treatments was statistical analysis by Anava, and continued byLowest Significant Difference Test to locate the difference. Normal fat ration did not influence coronaryartery wall thickness and coronary artery lumen diameter in rats. High fat ration increased coronaryartery wall thickness and chitosan could be decreased coronary artery wall thickness in rats. High fatration + chitosan that given simultaneously could be increased coronary artery lumen diameter in rats.Based on the result of this study, it was concluded that high fat ration was a major factor able to causeatherosclerosis and chitosan was able to prevent atheroma plaque formation.
Co-Authors , Parjanto Agus Slamet Agustina A. HANDAYAN Aries Bagus Sasongko Aziz Purwantoro Bambang Setiaji Bekti Sulistya Utami Djoko Santoso ENDANG S. SOETARTO Endang Semiarti G. Galih Hastari Wuryastuty Ixora Sartika Mercuriani Langkah Sembiring Nyoman Puniawati Parjanto Parjanto Sarkono Sarkono Sorta Basar Ida Simanjuntak Sorta Basar Ida Simanjuntak Sri Isdadiyanto Sri Isdadiyanto Subagus Wahyuono Tjut S. Djohan Tri R. Nuringtyas W. T. Artama W.T. Artama Wayan Tunas Artama Yoga Pratama Yuliana Retnowati