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All Journal HAYATI Journal of Biosciences Jurnal Fitopatologi Indonesia AGRIVITA, Journal of Agricultural Science Jurnal Agroteknos Jurnal Hilirisasi IPTEKS (JHI) Menara Perkebunan Agroplantae: Jurnal Ilmiah Budidaya dan Pengelolaan Tanaman Perkebunan Jurnal Mandala Pengabdian Masyarakat
Andi Khaeruni
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Earth & Planetary Sciences Engineering Environmental Science Health Professions Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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PATHOTYPE GROUPING OF Xanthomonas oryzae pv. oryzae ISOLATES FROM SOUTH SULAWESI AND SOUTHEAST SULAWESI Khaeruni, Andi; Wijayanto, Teguh
AGRIVITA, Journal of Agricultural Science Vol 35, No 2 (2013)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

Bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is an important rice disease, and has caused significant economic losses.  This research aimed to determine the pathotype grouping and the distribution of Xoo isolates of South and Southeast  Sulawesi. In order to obtain the information, 61 Xoo isolates of South Sulawesi and 29 isolates of Southeast Sulawesi were evaluated for their pathotype grouping against 5 diffential varieties. Research results showed that in South Sulawesi there were 2 pathotype groups, namely pathotype IV (32.79%) and pathotype VIII(67.21%).  Pathotype VIII was widely distributed over the Western and Central areas of South Sulawesi, whereas pathotype IV was widely distributed over the Southern area.  In Southeast Sulawesi, it was found 5 pathotypes, namely pathotypes IV (27.58%), VI (10.34%), VIII (13.79%), IX (20.68%), and X (27.58%), with a limited and scattered distribution pattern on several areas. These results indicate that  Xoo pathotype groups in South Sulawesi and Southeast Sulawesi are varied and tend to sift to more virulent pathotypes. Keywords: bacterial leaf blight, pathotype group, Xanthomonas oryzae pv. oryzae
Rapid Detection of Bacterial Pustule Disease on Soybean Employing PCR Technique with Specific Primers ANDI KHAERUNI; ANTONIUS SUWANTO; BUDI TJAHJONO; MEITY SURADJI SINAGA
HAYATI Journal of Biosciences Vol. 14 No. 2 (2007): June 2007
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.581 KB) | DOI: 10.4308/hjb.14.2.76

Abstract

A rapid polymerase chain reaction (PCR)-based procedure was developed for detection of Xanthomonas axonopodis pv. glycines, the causal agent of bacterial pustule disease on soybean. A set of primers was designed from partial sequence of the pathogenicity gene of X. axonopodis pv. glycines strain YR32. Specific PCR product of 490 base pairs was produced from strains of X. axonopodis pv. glycines originally from Indonesia as well as from Taiwan. No other pathovars and bacterial species among those tested showed amplification product under optimized PCR conditions. Shaking infected soybean leaves in phosphate buffer saline during six hours was proved to be an essential in order to increase cell number of the bacterial. The procedure was applicable and reliable for detecting of pathogens in infected plant materials. The procedure was proved to be more effective than that of conventional detection and could be of great help for monitoring of pustule bacterial disease in the soybean fields. Key words: Xanthomonas axonopodis pv. glycines, bacterial pustule disease, rapid detection, PCR, specific primer
Start up Paper Soap Business Based on Katuk Leaf (Sauropus androgyrus (l) merr) by Students in Mandala Waluya University Muhammad Nurdin; Haris Watoni; Andi Khaeruni; Maulidiyah Maulidiyah; Zul Arham; Irwan Irwan; La Ode Agus Salim
Jurnal Mandala Pengabdian Masyarakat Vol. 2 No. 2 (2021): Jurnal Mandala Pengabdian Masyarakat
Publisher : Progran Studi Farmasi STIKES Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (833.977 KB) | DOI: 10.35311/jmpm.v2i2.36

Abstract

Bahan anti jamur busuk buah kakao berbasis suspensi TiO2 telah disosialisasikan dan diaplikasikan pada lahan perkebunan kakao Kelompok Tani Mekohia, desa Lebo Jaya-Konawe Selatan. Sosialisasi dan aplikasi bahan anti jamur dilakukan melalui program pengabdian berbasis masyarakat. Pemilihan kelompok tani Mekohia sebagai mitra didasarkan pada fakta bahwa kelompok ini mengalami kesulitan dalam penanganan penyakit busuk buah kakao. Selain melakukan pembinaan berupa bimbingan teknologi, kegiatan ini juga bertujuan mengenalkan dan menerapkan sebuah produk teknologi baru dalam pencegahan dan penanganan penyakit busuk buah kakao yang disebabkan oleh jamur Phytopthora palmivora (P. palmivora). Metode kegiatan terdiri dari tiga tahapan penting, meliputi (i) focus group discussion (FGD), (ii) tahap pembimbingan teknis pembuatan suspensi TiO2 dan cara penggunaannya, dan (iii) penguatan konsep pengendalian hama dan penyakit tanaman kakao. Kegiatan ini menghasilkan produk bahan anti jamur busuk buah kakao berbasis suspensi TiO2 yang diaplikasikan secara langsung pada lahan perkebunan kakao mitra. Luaran lainnya berupa kemampuan kelompok tani Mekohia dalam penyiapan suspensi TiO2 secara mandiri. Indikator keberhasilan kegiatan diamati dari tingkat keseriusan mitra dalam berdialog dan kemampuan mitra dalam menerapkan suspensi TiO2. Akhir kegiatan ini menghasilkan Memorandum of Understanding (MoU) antara tim pengabdi dengan mitra Mekohia tentang penggunaan bahan anti jamur suspensi TiO2 secara berkala.
EKSPLORASI DAN KARAKTERISASI AZOTOBACTER INDIGENOUS UNTUK PENGEMBANGAN PUPUK HAYATI TANAMAN PADI GOGO LOKAL DI LAHAN MARJINAL ANDI NURMAS; NOFIANTI -; ABDUL RAHMAN; ANDI KHAERUNI
Jurnal Agroteknos Vol 4, No 2 (2014)
Publisher : Jurnal Agroteknos

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.767 KB)

Abstract

Azotobacter is bacterium that has the ability to fix nitrogen and produce hormone IAA, thus potentially acts as biofertilizer agent. There are differences in chemical, biological and other characters of Azotobacter strains. Some strains have higher ability to fix nitrogen than the others. Exploration and characterization are important to be done because the bacteria that provide nutrients and live free rhizosphere regions, possible have different abilities.  In addition to N2 fixing, growth hormone production, phosphate dissolving, they are also tolerant to a certain temperature and pH. The purpose of the research was to obtain information and the latest data on indigenous Azotobacter that potential as a source of biofertilizer, and triggering factor for upland rice growth on marginal lands in Southeast Sulawesi.  Exploration from several locations has selected 21 Azotobacter isolates. The test results indicated that the 21 Azotobacter isolates have the ability to produce IAA, dissolve phosphate and stable at pH 5.0-7.0. All 21 isolates tested had the ability to survive at 40oC, eight isolates i.e. LT2D1, LT2d2, LU2c, RG4c, MP1f, LT2d3, ML1j, and RR8awere able to survive at a temperature of 45o C, and LT2d1 isolate survived at temperatures 50oC. The results of the evaluation of the wet weight of upland rice seedlings selected 10 isolates that were: KU6e, MS3e, RG4c, RR8b1, LU2c, RB4b, MS3f, LU2c1, RJ5e, RR8b2 and evaluation of seedling dry weight selected 5 isolates that were: RB4b, LU2c, RJ5e, RR8b2, LT2d1. Keywords:      exploration, characterization, indigenous Azotobacter, local upland rice
ISOLASI DAN KARAKTERISASI BAKTERI MANOLITIKASAL BONGGOL POHON SAGU SRI WAHYUNI; LIANTO -; ANDI KHAERUNI
Jurnal Agroteknos Vol 4, No 3 (2014)
Publisher : Jurnal Agroteknos

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.153 KB)

Abstract

Sago Processing in Southeast Sulawesi often generate waste in the form of pulp and tuberswhich contains lignin, cellulose, starch, minerals, and vitamins that can be used as a source of carbon and energy for growth of microorganisms, so it is likely to get microbes, including mannolitic bacteria that are useful for human life. The aim of this study was to isolate and characterize the biochemical properties of mannolitic bacteria originated fromwaste of sago hump in Konawe, Southeast Sulawesi Province. Isolation was done using serial dilution method and then spread over the surface of Nutrient agar medium and Mannan enrichment. Bacterial isolates showing high mannose activity were characterized mannose physiologically and biochemically. From this research, 6 mannolitic isolates originated from hump of sago waste samples from South Konawe were obtained. BLS.11-01 and BLS.11-02 mannolitic bacterial isolates had a strong mannolitic activity, with mannolitic index value of 2.3 and 2.0, respectively. Presumably, the two isolates were gram-positive bacteria, belonging to the same genus.
DETEKSI POTYVIRUS PADA NILAM (Pogostemon Cablin (BLANCO) BENTH) DENGAN TEKNIK ELISA DI SULAWESI TENGGARA MUHAMMAD TAUFIK; ASMAR HASAN; ANDI KHAERUNI; GUSNAWATY HS - -; SARAWA MAMMA
Jurnal Agroteknos Vol 4, No 1 (2014)
Publisher : Jurnal Agroteknos

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Abstract

Mosaic symptoms were observed on Patchouli (Pogostemon cablin) around North Kolaka and Kolaka, Southeast Sulawesi during surveys in early 2012. Indirect-ELISA based detection was conducted using symptomatic leaf samples. The objective of the research was to determine disease incidence of Potyvirus in several farms of Patchouli plant in Southeast Sulawesi. The results showed that Patchouli plant (Pogostemon cablin) was found to be infected with Potyvirus disease showing mosaic symptoms and malformation on the leaf samples i.e. in Amotowo and Boro-Boro Villages of subdistrict Boro-Boro, and Landabaro Village, Mowila subdistrict of South Konawe regency; Asinua Village of subdistrict Unaaha, Lambuya district of subdistrict Lambuya, and Bungguosu district, Konawe subdistrict of Konawe regency; and Anduonohu district, Poasia subdistrict of Kendari regency. This is the first report on Potyvirus infection on patchouli in Southeast Sulawesi. Keywords: Inderect-ELISA, mosaic, Potyvirus, Pogostemon cablin
PATHOTYPE GROUPING OF Xanthomonas oryzae pv. oryzae ISOLATES FROM SOUTH SULAWESI AND SOUTHEAST SULAWESI Andi Khaeruni; Teguh Wijayanto
AGRIVITA, Journal of Agricultural Science Vol 35, No 2 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i2.256

Abstract

Bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is an important rice disease, and has caused significant economic losses.  This research aimed to determine the pathotype grouping and the distribution of Xoo isolates of South and Southeast  Sulawesi. In order to obtain the information, 61 Xoo isolates of South Sulawesi and 29 isolates of Southeast Sulawesi were evaluated for their pathotype grouping against 5 diffential varieties. Research results showed that in South Sulawesi there were 2 pathotype groups, namely pathotype IV (32.79%) and pathotype VIII(67.21%).  Pathotype VIII was widely distributed over the Western and Central areas of South Sulawesi, whereas pathotype IV was widely distributed over the Southern area.  In Southeast Sulawesi, it was found 5 pathotypes, namely pathotypes IV (27.58%), VI (10.34%), VIII (13.79%), IX (20.68%), and X (27.58%), with a limited and scattered distribution pattern on several areas. These results indicate that  Xoo pathotype groups in South Sulawesi and Southeast Sulawesi are varied and tend to sift to more virulent pathotypes. Keywords: bacterial leaf blight, pathotype group, Xanthomonas oryzae pv. oryzae
PEMBERDAYAKAN MASYARAKAT DESA AUNUPE DALAM PENGELOLAAN HAMA DAN PENYAKIT TANAMAN YANG RAMAH LINGKUNGAN Andi Khaeruni; Rahayu Rahayu; Gusti Ayu Kade Sutariati; Tresjia C. Rakian
Jurnal Hilirisasi IPTEKS Vol 3 No 1 (2020)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (558.37 KB) | DOI: 10.25077/jhi.v3i1.382

Abstract

Aunupe Village, Wolasi District is one of the villages for the development of organic vegetables in Konawe Selatan Regency. The frequent occurrence of high levels of pest and disease attacks is the main reason for the use of synthetic pesticides that are not justified in organic farming systems. On the other hand, in the same location, there is a group of women farmers who are not productive. Both of these problems can be overcome if the farmer women's group produces quality biological agents that can be utilized by horticultural farmers in an environmentally friendly cultivation system. The implementation of the PKM Program in Aunupe Village is very appropriate as a means and media for transferring technology on how to increase the productivity of environmentally friendly vegetable plants by developing organic farming techniques. Control of pests and plant diseases by using biological agents and botanical pesticides is a wise choice in efforts to increase agricultural production with high economic value while maintaining biodiversity to support sustainable agricultural cultivation, especially in the cultivation of vegetable crops as a source of community nutrition.
PENERAPAN TEKNOLOGI BUDIDAYA TANAMAN RAMAH LINGKUNGAN UNTUK PRODUKSI SAYURAN SEHAT DI KELOMPOK WANITA TANI WIJAYA KUSUMA KELURAHAN KAMBU KOTA KENDARI Andi Khaeruni; Terry Pakki; Asniah Asniah; Nur Isnaini Ulfa; Mirza Asriyanti Arsyad; Agustono Agustono
Jurnal Hilirisasi IPTEKS Vol 5 No 1 (2022)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jhi.v5i1.526

Abstract

During the current Covid-19 pandemic, the need for healthy food, including vegetables, is significant to increase the body's immunity to free from infection with the disease. The availability of healthy vegetables can be obtained through using the yard by growing vegetables organically. This activity aims to increase community participation in developing organic agriculture and supporting sustainable agriculture during the Covid-19 pandemic. The target of this program is the community of Kambu Village and the city government of Kambu Village, Kambu District, Kendari City. The activities carried out involve participating in various parties (city governments, communities, students, and academics) through counseling, training, demonstrations, and making demonstration plots for organic vegetable cultivation. This guidance hopes that sustainable organic farming will be realized through this guidance. The results of this activity indicate that community participation, especially among members of the women's farmer group Wijaya Kusuma, Kambu Village, has increased in the use of limited land to cultivate healthy vegetables. Public awareness and knowledge about the importance of applying organic fertilizers and vegetable pesticides (eco-enzymes) are increased through these activities. Increased organic fertilizers and eco-enzymes for household-scale vegetable cultivation by the Wijaya Kusuma Farmer Women's Group, Kambu Village, Kambu Village, Kambu District, Kendari City. Availability of healthy food ingredients in the form of sustainable organic vegetables. The collaboration between the city government and academia is increasingly closely intertwined through the activities carried out.
Agensia hayati dan Arachis pintoi memacu pertumbuhan tanaman lada (Piper nigrum) dan mengurangi kejadian penyakit kuning Biocontrol agents and Arachis pintoi promote the growth of black pepper (Piper nigrum) and reduce the incidence of yellow disease Muhammad TAUFIK; Andi KHAERUNI; Abdul WAHAB; . AMIRUDDIN
E-Journal Menara Perkebunan Vol 79, No 2: Desember 2011
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.92 KB) | DOI: 10.22302/iribb.jur.mp.v79i2.58

Abstract

AbstractYellow disease is a complex disease caused byFusarium sp., Phytophthora sp., and nematodes. Infectedplants were quickly killed and were difficult for replanting,causing significant losses for the growers. Various controlmethods were examined including the use of bioconrolagents and cover crop Arachis pintoi. The researchobjective was to determine the ability of biocontrol agentsand A. pintoi to improve pepper growth and reduce yellowdisease incidence on pepper plants in the field. Researchresults showed that the treatment of biocontrol andA. pintoi promoted vegetative growth of pepper plants, andincreased pepper height for up to more five times, andreduced yellow disease incidence to 30%AbstrakPenyakit kuning merupakan penyakit kompleks yangdisebabkan oleh Fusarium sp., Phytophthora sp. dannematoda parasit. Tanaman sakit mengalami kematianyang cepat dan kebun yang telah terinfeksi sulit untukditanami kembali, sehingga mengakibatkan kerugian yangnyata terhadap petani. Berbagai cara pengendalian telahdiuji termasuk penggunaan agens hayati Plant GrowthPromoting Rhizobacteria (PGPR), Trichoderma sp. dantanaman Arachis pintoi. Tujuan penelitian adalahmengetahui kemampuan agensia hayati dan Arachis pintoidalam meningkatkan pertumbuhan dan mengurangikejadian penyakit kuning pada tanaman lada di lapang.Hasil penelitian menunjukkan bahwa perlakuan agenshayati dan A. pintoi meningkatkan tinggi dan jumlah dauntanaman lada lebih dari lima kali serta mempercepatmunculnya sulur dibandingkan dengan kontrol danfungisida. Aplikasi Trichoderma sp. yang dikombinasidengan A. pintoi menekan kejadian penyakit kuning hampir30%.
Co-Authors . Amiruddin Abdul Rahman Abdul Wahab Agustono Agustono Andi Nurmas Antonius Suwanto dan Meity S. Sinaga . Budi Tjahjono Andi Khaeruni R Asmar Hasan Asniah Asniah Budi Tjahjono GUSNAWATY HS, GUSNAWATY Gusti Ayu Kade Sutariati Haris Watoni Irwan Irwan La Ode Agus Salim La Ode Santiaji Bande LIANTO - MARIADI MARIADI Maulidiyah Maulidiyah MEITY SURADJI SINAGA Mirza Asriyanti Arsyad Muhammad Botek Muhammad Nurdin MUHAMMAD TAUFIK NOFIANTI - NOOR AIDAWATI NUR ISNAINI ULFA PURNAMA HIDAYAT Rahayu Mallarangeng Rahayu Rahayu SARAWA MAMMA SEDYO HARTONO Sri Wahyuni Syair Syair Teguh Wijayanto TERRY PAKKI Tiara Hafidsya Tresjia C. Rakian Vit Neru Satrah Waode Siti Anima Hisein Zul Arham