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PEMANFAATAN TELUR KEONG EMAS (Pamacea canalicula) SEBAGAI MEDIA PERTUMBUHAN BAKTERI Staphylococcus, E. coli dan Lactobacillus Hayyun Maghfiroh; Sartini Sartini; Rahmiati rahmiati
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 2, No 2 (2020): November
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jibioma.v2i2.316

Abstract

Penelitian tentang Pemanfaatan Telur Keong Emas (Pamacea canalicula) Sebagai Media Pertumbuhan Bakteri  Staphylococcus, E.  coli  dan  Lactobacillus dilakukan untuk  mengetahui pertumbuhan bakteri pada media telur keong emas. Metode penelitian yang digunakan dalam penelitian ini adalah  metode deskriptif. Penelitian ini terdiri dari tiga tahapan yaitu a) Pembuatan media telur keong emas, b) Penanaman Koloni Bakteri  Staphylococcus, Lactobacillus dan E.coli pada Media Telur Keong Emas dan c) Pewarnaan Gram Pada Biakan Bakteri Staphylococcus, Lactobacillus dan E.coli   dalam media telur keong emas. Hasil penelitian menunjukkan bahwa untuk bakteri E.coli, dan Staphylococcus ditemukan adanya tanda-tanda pertumbuhan bakteri baik secara fisik terlihat adanya perubahan warna, kekeruhan serta terdapat buih pada media  dan mikroskopis terlihat adanya bakteri pada slide yang telah diwarnai dengan pewarnaan gram. Tetapi untuk bakteri Lactobacillus tidak ditemukan tanda-tanda pertumbuhan bakteri baik secara fisik dan mikroskopis. Maka dapat disimpulkan bahwa telur keong emas memiliki potensi sebagai media pertumbuhan bakteri E.coli dan Staphylococcus
Hubungan antara Diabetes Mellitus dengan Hipertensi pada Pralansia dan Lansia di Puskesmas Rambung Kota Tebing Tinggi Hormarita Saragih; Meida Nugrahalia; Sartini Sartini
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 1, No 2 (2019): November 2019
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (298.955 KB) | DOI: 10.31289/jibioma.v1i2.163

Abstract

The purpose of this study was to determine the relationship between Diabetes Mellitus and Hypertension in Pralansia and the Elderly in Rambung Puskesmas Tebing Tinggi. The research method is carried out descriptively, which is a study conducted with the main objective to make a picture or description of a situation objectively to solve or answer a problem. Based on the results of the study that the relationship between Diabetes Mellitus and Hypertension in Rambung Puskesmas Tebing Tinggi, was quite strong with a value of r = 0.80. The correlation between the disease with 5 factors namely age, sex, blood sugar level, systolic blood pressure and diastolic blood pressure as a whole is very high at r = 0.83. Patients suffering from diabetes mellitus and hypertension are more found in the female sex than men, and people who have a history or hereditary factors of hypertension and diabetes mellitus more than those who have no history, and more patients who first had new diabetes mellitus followed by hypertension
Uji Cemaran Bakteri pada Bawang Putih Giling yang Dijual di Pasar Tradisional Kecamatan Galang Listrahot Tondang; Abdul Karim; Sartini Sartini
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 2, No 1 (2020): Mei 2020
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.594 KB) | DOI: 10.31289/jibioma.v2i1.223

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Garlic is one of the spices or spices that has a delicious tastes and aroma and garlic has many health  benefits. The ingredients purchased in the traditional market need to be kept clean to avoid bacterial contamination that cause disease. The study aims to determine bacterial contamination of ground garlic sold in the traditional market of Galang. The research method is descriptive to see the number of colonies and the characteristic of the types of bacteria that contaminate onions the white sold in the traditional market in Galang.. The result of laboratory samples obtanied 2 sample contaminated with Klebsiella oxytoca bacteria and 6 sample contaminated with Enterogenous aerogenosa bacteria. Conclutions  obtained and the results of this study are ground garlic sample sold in the  traditional market of galang distric. It can be concluded that 2 samples were polluted by Klebsiella oxytoca bacteria and 6 samples contaminated with aerogenous Enterobacter bacteria feasible consumption of larvae according to the  regultions of BPOM no. HK.00.06.1.53.4011 of 2009 and Indonesian National Standart no.7338 of 2009 on herbs and spices the number of total plates is not greater and the threshold of 1x106 colony/gram
Infeksi Cacing Nematoda Usus Pada Anak Kelas 1 dan 2 Sekolah Dasar Oki Irawati; Sartini Sartini; Ida Fauziah
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 3, No 1 (2021): Mei 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jibioma.v3i1.538

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Worm disease is still one of the health problems in Indonesia. Personal hygiene such as playing in the school ditch, on the ground, playing dirty and some children do not use footwear, long nails and the habit of not washing their hands with soap before and after playing, thereby increasing the risk of intestinal worms in children. This study aims to determine the percentage and types of intestinal nematode worm eggs that infect elementary school children grades 1 and 2 Raudathul Jannah Foundation, Penraujan Private Elementary School, Sunggal District, Deli Serdang Regency. The method used in this study was direct stool examination. Based on the results of the examination of 48 samples of elementary school students in grades 1 and 2, it was found that 3 samples were positively infected with intestinal nematode worm eggs with a percentage of 6.25% and there were 2 types of worm eggs that infect, namely Ascaris lumbricoides and Trichuris trichiura. Where sample (S 12) was infected with Trichuris trichiura worm eggs, sample (S 37) was infected with Ascaris lumbricoides worm eggs and sample (S 46) was infected with both types of worm eggs, namely Ascaris lumbricoides and Trichuris trichiura.
Uji Efektivitas Cendawan Metarhizium anisopliae Terhadap Daya Bunuh Instar 2 Dan Instar 4 Larva Ulat Spodoptera Litura Pada Tanaman Sawi Hijau (Brassica juncea L.) Di Laboratorium One May Sunday Nababan; Sartini Sartini; Riyanto Riyanto
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 2, No 1 (2020): Mei 2020
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (332.451 KB) | DOI: 10.31289/jibioma.v2i1.184

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Pests are one of the factors that reduce the production and quality of cultivated plants, for example grayak caterpillars (Spodoptera litura) which attack mustard plants. Grayak caterpillars (Spodoptera litura) can be controlled using chemical compounds (insecticides), can be manually collected by hand and can also be biologically controlled, for example with mushrooms. The purpose of this study was to examine the effectiveness of the fungus Metarhizium anisopliae on Spodoptera litura caterpillar larvae in green mustard plants (Brassica juncea L.). The caterpillar used in this study is the Instar-2 (I₁) and Instar-4 (I₂) phase caterpillars. The concentration of fungi was inoculated were 10-2 (M₁), 10 -4 (M₂), and 10 -6 (M₃), and without fungi as a control (M₀). The design used was Factorial RAL with 3 replications. The experimental results showed that spore density M₁, M₂, and M₃ were quite effective at killing the caterpillar both on instar-2 (I₁) and on instar-4 (I₂
Proteinuria pada Penderita Gagal Ginjal Kronik di Rumah Sakit Martha Friska Multatuli Medan Tahun 2015-2016 Juliana Ruminta Sijabat; Sartini Sartini; Abdul Karim
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 1, No 2 (2019): November 2019
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (289.539 KB) | DOI: 10.31289/jibioma.v1i2.166

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This study aims to determine the presence or absence of protein in urine in patients with chronic renal failure. From the results of the study it was found that patients with chronic kidney failure in Martha Friska Multatuli General Hospital Medan from the 80 most patients in positive +++. Many patients with chronic kidney failure are male patients (62.5%) compared to female patients (37.5%) and the results of the examination based on age increase with age, ie at the age of 45 years and above. This type of research is descriptive analytic. Data is taken by recording medical record data of patients with chronic kidney failure based on age, sex and year. The study was conducted from January 2015 to December 2016. The place for conducting the research was conducted at Martha Friska Multatuli General Hospital (RSU) Medan. The study sample was urine samples from all patients with chronic renal failure. It can be concluded that the picture of protein in urine in patients with chronic kidney failure increases every year.
Infeksi Jamur Dermatofita Pada Penderita Mikosis Kuku Winda Irawati Zebua; Kiki Nurtjahja; Sartini Sartini
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 3, No 1 (2021): Mei 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jibioma.v3i1.539

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Dermatophytes are a group of fungi that cause nail abnormalities. This study aims to determine dermatophyte fungal infections in patients with nail mycoses. The method used in this study is descriptive and the sample was taken by purposive sampling with the criteria of damaged toenails, with characteristics: nails are not shiny, brownish to black from the base to the tip then the nails are examined directly with 10% KOH and cultured using media Sabouraud Dextrose Agar (SDA). The fungi isolated on slanted agar containing SDA and each fungal species were then identified using preparations for microscopic examination. The results showed that from 30 samples, 8 (eight) nail samples were positive for dermatophytes, namely: 5 (five) people infected with the fungus Tricophyton mentagrophytes species, 2 (two) people infected with the Trichophyton rubrum fungus, and 1 (one) person infected with Epidermophyton floccosum..
Isolasi Bakteri Asam Laktat (BAL) dari Saluran Pencernaan Ikan Nila (Oreochromis niloticus) dan Kemampuannya Dalam Menghambat Staphylococcus aureus dan Shigella sp. Alpina Bukhori; Sartini Sartini; Rahmiati rahmiati
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 2, No 1 (2020): Mei 2020
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (294.509 KB) | DOI: 10.31289/jibioma.v2i1.185

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Lactic acid bakteria have characteristics that are able to ferment sugars or carbohydrates and produce lactic acid as the final product of fermentation. The purpose of this research was to isolates the lactic acid bacteria from the digestive tract of tilapia fish and to investigate the inhibitory power produced by lactic acid bacteria in inhibiting the growth of Staphylococcus aureus and Shigella sp. Isolation of lactic acid bacteria was done by using MRSA medium and to see the ability of lactic acid bacteria isolates in inhibiting the growth of Staphylococcus aureus and Shigella sp then used disc diffusion method. The isolates obtained were then characterized by morphology and biochemistry. From the research obtained two lactic acid bacteria that is sp1 and sp2. Lactic acid bacteria sp1 and sp2 are able to inhibit Staphylococcus aureus and Shigella sp with the biggest inhibition to Staphylococcus aureus by sp2 of 8.75 mm and the biggest inhibition of Shigella sp is shown by sp2 that is equal to 7.16 mm. Based on the results of isolation and characterization done in this research can be concluded that lactic acid bacteria of the digestive tract of fish tilapia sp1 and sp2 capable in inhibiting Staphylococcus aureus with a the number of 8.75 mm and Shigella sp with a of 7.16 mm.
Isolasi dan Karakteristik Bakteri Endofit pada Akar Pepaya (Carica papaya L) Guspi Wilda Sari Sianipar; Sartini Sartini; Riyanto Riyanto
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 2, No 2 (2020): November
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jibioma.v2i2.312

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Bakteri endofit adalah mikroorganisme yang seluruh atau sebagian hidupnya berada dalam jaringan tumbuhan (Akar, Batang, Daun, dan Bunga). Tujuan penelitian ini adalah untuk mengetahui karakteristik bakteri endofit pada akar pepaya (Carica papaya L) secara makroskopis dan mikroskopis. Penelitian ini menggunakan metode  deskriptif yang dilakukan dengan tiga tahapan yaitu prepasi sampel dan media, isolasi dan karakterisasi melalui uji biokimia. Hasil penelitian ditemukan 2 isolat bakteri endofit yang diberi kode AP1 dan AP2. Pewarnaan gram menunjukkan kedua isolat bakteri endofit berbentuk basil gram positif yang berwarna ungu. Dari hasil penelitian ini menunjukkan karakteristik morfologi dan uji biokimia isolat bakteri endofit diduga merupakan genus Bacillus.
Uji Aktivitas Antibakteri Ekstrak Daun Pepaya (Carica papaya) dan Ekstrak Kunyit Putih (Curcuma zedoaria) Terhadap Escherichia coli dan Staphylococcus epidermidis Herlina Lumbantobing; Sartini Sartini; Rahmiati Rahmiati
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 4, No 1 (2022): Mei 2022
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jibioma.v1i1.1226

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This study aims to determine the active ingredients contained in papaya leaf extract (Carica papaya) and white turmeric extract (Curcuma zedoaria) and their antimicrobial activity against Escherichia coli and Staphylococcus epidermidis. This research was conducted using experimental methods at the Laboratory of Molecular Biology, Faculty of Medicine, University of Prima Indonesia. The research data were analyzed using a completely randomized design approach. Papaya leaf extract and white turmeric were made at different concentrations of 25%, 50%, 75% and 100% and the positive control was the antibiotic chloramphenicol (2 g). The results showed that papaya leaf extract contains active compounds of flavonoids, alkaloids, terpenoids and saponins. While white turmeric extract contains flavonoids, steroids, tannins and saponins. Papaya leaf extract 100% is the best treatment to suppress the growth of bacteria Echerichia coli and Staphylococcus epidermidis with an average inhibition of 14,110 mm and 14,707 mm, respectively. The administration of 100% white turmeric extract was the best treatment to suppress the growth of E. coli and S. epidermidis with an average inhibition of 14,307 mm and 13,743 mm, respectively.
Co-Authors Abdul Karim Al Makin Al Makin, Al Makin Alpina Bukhori Amriani Amriani Amriani Amriani, Amriani Andi Indardaya, Andi Anisantaria Anisantaria Anisantaria, Anisantaria Daraini Mahdhalita Djuretna Adi Imam Muhni, Djuretna Adi Imam E. Harso Kardhinata E. Harso Kardhinata Ellen Lumisar Panggabean Emilia Utomo, Emilia Eriani Sahara Eriani Sahara, Eriani Fastabiqul Khairad Ferdinand Susilo Fhahri Mubarak Fitriani, Rita Green Juninas Simbolon Green Juninas Simbolon, Green Juninas Guspi Wilda Sari Sianipar Haeriah Haeriah, Haeriah Hayyun Maghfiroh Heddy Shri Ahimsa Putra Heni Febriani, Heni Henny Yuslina Herlina Lumbantobing Hormarita Saragih I Gede Arya Sucitra Ida Fauziah Ida Fauziah, Ida Ilham Ramadhan Nasution Indri Rizki Rahmasari Irawan, Maya Novita Sari Juliana Ruminta Sijabat Kardhinata, E. Harso Karim, Abdul Khairani khairani khairani, Khairani KIKI NURTJAHJA Kuswardani, Retna Astuti Listrahot Tondang Lubis, Rosliana Lulu Fatma Dewi Marliana marliana marliana, Marliana Marti Silfia Maya Novita Sari Irawan Meida Nugrahalia Meida Nugrahalia Meida Nugrahalia, Meida Muhammad Rahmatullah, Muhammad Muhammad Usman Muhammad Usman, Muhammad Munte, Nuriana Novianti Novianti Nugrahalia, Meida Nuraini Nuraini Nurhalimah Nurhalimah Nuriana Munte Oki Irawati Okianna Okianna One May Sunday Nababan Purnama Dewi Rohana Purnawanti, Dia Rahmiati Rahmiati Rahmiati Rahmiati Ratnawati ratnawati ratnawati, Ratnawati Retna Astuti Kuswardani Rita Fitriani Riyanto Riyanto Riyanto Rosliana Lubis Rosliana Lubis, Rosliana Royman S Panjaitan Saipul Sihotang Siti Rubiatik Siti Rubiatik, Siti Sukoso Sukoso Sulastri Sulastri Sulastri Sulastri Vera Ananda Harahap Winda Irawati Zebua Yeni Artika Yuslina, Henny