Biosaintifika: Journal of Biology & Biology Education
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
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<![CDATA[Traditional Usages of Taro (Colocasia spp.) by Ethnic Communities in Borneo ]]>
<![CDATA[Oktavianingsih, Linda]]>;
<![CDATA[Suharyanto, E.]]>;
<![CDATA[Daryono, Budi Setiadi]]>;
<![CDATA[Purnomo, Purnomo]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i2.9956
<![CDATA[Borneo has a a wealth of various flora, including the Aroids (Araceae). Taro is one of the Aroids that cultivated and used as food crop since a long time ago by the people in Borneo. This study aimed to determine the utilization of Taros traditionally by several ethnic communities in Borneo. The research used Survey Explorative Method with Direct Interview Technique in the field. Taro samples were taken from various habitats of Banjar, Dayak, Kutai, Malay, Bugis, Toraja and China ethnic. The results showed that Taro species widely used are : Colocasia esculenta var. esculenta and var. antiquorum, Colocasia affinis and wild of Colocasiaesculenta. C. esculenta var. esculenta and var. antiquorum is used as subsistence food crop and vegetables. Besides, it is used as medicine such as high blood pressure lowering and for consumption of diabetics. Parts of the plants consumed include leaves, petiole, corm and stolon. C. affinis is used as ornamental plants of the home garden, while wild C. esculenta is used as animal fodder. Taro in Borneo have a considerable variation of traditional cultivars and vernacular names. Fifty eight traditional cultivars from 5 different habitat to be used by some ethnics.]]>
<![CDATA[Characterization of Ethanolic Extract of Streptomyces sp. as a Pancreatic Lipase Inhibitors Produced by Endophytic Streptomyces sp. AEBg12 ]]>
<![CDATA[Fitri, Lenni]]>;
<![CDATA[Meryandini, Anja]]>;
<![CDATA[Iswantini, Dyah]]>;
<![CDATA[Lestari, Yulin]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i2.8907
<![CDATA[Endophytic Streptomyces sp. AEBg12 isolated from Zingiber cassumunar (Bangle) is known to produce pancreatic lipase inhibitory compound. However, the characteristics of this active compound has not been reported yet. This study aimed to determine the characteristics of pancreatics inhibitory compound produced by Streptomyces sp. AEBg12 and to assess the role of endophytic actinobacteria in producing pancreatic lipase inhibitor using endophytic-free bangle tissue culture, wild bangle and compared with the activity of Streptomyces sp. AEBg12 endophytes. Supernatant of Streptomyces sp. AEBg12 was extracted using ethanol, ethyl acetate, and n-hexane solvents. Toxicity test was performed using larvae of shrimp Artemia salina. The results showed that the best solvent to obtain pancreatic lipase inhibitor compounds was ethanol. Phytochemical analysis showed that ethanolic extract of endophytic Streptomyces sp. AEBg12 contained flavonoids. IC50 value of ethanol extract was 180.83 g/ml. The result of TLC showed that ethanolic extract of Streptomyces AEBg12 had a blue luminescence band indicated that there were either flavone, flavanones, flavonols or isoflavones. Inhibitory activity of Streptomyces sp. AEBg12 was higher than wild bangle and bangle tissue culture. The information from this study can be be used as a basic data for further characterization of the active compound, which might be developed as an antiobesity agent through its pancreatic lipase inhibitory activity.]]>
<![CDATA[Correlation of Physical-Chemical Parameters to Total Coliform Value in Jawi River, Pontianak, West Kalimantan ]]>
<![CDATA[Rahmawati, Rahmawati]]>;
<![CDATA[Fitria, Laili]]>;
<![CDATA[Syafitri, Liza]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i2.10187
<![CDATA[Coliform bacteria can be used as an indicator of the presence of pathogenic bacteria, such as E.coli bacteria that cause diarrhea. The aimed of this study is to determine the relationship between physical-chemical parameters namely temperature, pH, DO and BOD to the density of coliform bacteria in Jawi River, Pontianak. The sampling was conducted at one point each in the upstream, midstream and downstream area of the Jawi River during two tidal conditions of the Kapuas River in September 2016 at 09:40 (at low tide) and at 15:40 (at high tide). The correlation of physical-chemical parameter to coliform value was tested Pearson Product Moment. The results showed that coliform bacterial density increased from upstream to downstream with 150-1500 MPN/100 ml at high tide and 930-11000 MPN/100 ml at low tide. The results showed that the coliform bacterial density value had a positive relation with pH and BOD parameters and negative relation with temperature and DO parameters. So, it can be concluded that there is a correlation between physical parameters, such as temperature and chemical parameters such as pH, DO and BOD to microbiological parameters especially Coliform bacterial density. The benefit of this study is to give information about water quality of Jawi River and its correlation with density of Coliform bacterial, so that people are expected to pay more attention to the use of clean water to avoid the disease caused by coliform.]]>
<![CDATA[Alteration of Gills and Liver Histological Structure of Cyprinus carpio Exposed to Leachate ]]>
<![CDATA[Pribadi, Tri Dewi Kusumaningrum]]>;
<![CDATA[Syahidah, Dzikrina]]>;
<![CDATA[Harjanti, Sairandri Dyah]]>;
<![CDATA[Malini, Desak Made]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i2.8972
<![CDATA[One of the main problems in the waste management in Indonesia is the treatment of leachate, which mostly dumped to the river This research is aimed to obtain information of histological alteration in gills and liver of C. carpio L. exposed to leachate. Measurements on the water quality parameters comprised water temperature, pH, and dissolved oxygen (DO). This research was conducted by exposing leachate to C. carpio for 96 hours. The concentration of leachate were 0 ppm, 80 ppm, and 100 ppm. Histological preparation were made on the gills and liver using 10% fixative Neutral Buffered Formalin and Ehrlich Hematoxylin-Eosin staining with qualitative observation descriptive analyses for discussion. The result showed that increasing water temperature is directly proportional to the leachate concentration in the aquaria, while the value of pH and DO inversely proportional to the leachate concentration. Damages on the gills with 80 ppm leachate concentrasion were identified as follows: fusion of secondary gill filaments and hyperplasia of epithelial cell, along with karyorrhexis and hydropic degeneration on the liver. Damages on the gills of fishes exposed to leachate with 100 ppm concentrasion were identified as follows: fusion of secondary gill filaments, hyperplasia of epithelial cell, congestion, and edema along with karyorrhexis, hydropic degeneration and melanomacrophage centre (MMC) found on the liver. The results of this study can be used as an overview of the impact of an environmental pollution by leachate as indicated from histological damage to the gills and liver of C. carpio, thus contribute significan information to aquaculture sector and endorse better waste management]]>
<![CDATA[Seedling Production of Pak Choy (Brassica rapa L.) using Organic and Inorganic Nutrients ]]>
<![CDATA[Priadi, Dody]]>;
<![CDATA[Nuro, Fiqolbi]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i2.8537
<![CDATA[Pak Choy or Bok Choy (Brassica rapa L. var. chinensis) is one of favorite Chinese leafy vegetable for various dishes in Indonesia. In this study, it was used as a plant model to identify the appropriate organic hydroponic nutrient solution for leafy vegetable seedling production. The seed was sown on rock wool slabs submerged with 200 ml of a nutrient solution containing biofertilizer of Beyonic StarTmik@Lob (0, 25, 50, 75, and 100%). commercial hydroponic solution (0, 25, 50, 75, and 100%) and its combination (25, 50, and 75%). The experiments were arranged in a CRD. Meanwhile, the obtained data was analyzed using ANOVA followed by DMRT. The relationship among growth parameters was observed using Pearson correlation analysis. The result of the study showed that the combination of organic and inorganic nutrient (25% Beyonic StarTmik@Lob and 75% commercial hydroponic solution) resulted in the highest seedling growth parameters and leaf indices as well as the perfectly positive correlations among growth parameters. This result indicated that the use of organic nutrient alone was not appropriate for hydroponic seedling production of Pak Choy. Therefore, further study needs to be done to identify the hydroponic solution without inorganic nutrients towards the organic vegetable production.]]>
<![CDATA[Antioxidant Activity of Dominant Plants Species in Obat Pahit from Lingga Malay Ethnic in Riau Archipelago ]]>
<![CDATA[Fitmawati, Fitmawati]]>;
<![CDATA[Sofiyanti, Nery]]>;
<![CDATA[Roza, Rodesia Mustika]]>;
<![CDATA[Isnaini, Isnaini]]>;
<![CDATA[Irawan, Yulisa Resti]]>;
<![CDATA[Winata, Dhaniel Ridho]]>;
<![CDATA[Dewi, Awal Prichatin Kusumo]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i2.9808
<![CDATA[Obat Pahit is a potion that has been long commonly consumed by Lingga Malay society for generations as stamina keeper. The most dominant plants found in the packaging of the Obat Pahit were namely Bauhunia semibifida, Cnestis palala and Penawa Root (3 species). This research aimed to investigate and determine activity of antioxidant contents in Obat Pahit from five Traditional Medicine Practitioners (TMPs) in the district of Lingga. The tested samples were mashed then being soaked into 2 types of solvent: distilled water and methanol, containing HCl 1%. DPPH method was also used in this research. Quantitatively antioxidant activity test of Obat Pahit from the five TMPs by using methanol solvent had extremely highest activity compared to the distilled water solvent. The test, using TLC plate by spraying the extract from three dominant plants with 0.1 mM of DPPH solution, produced a pale-yellow spots at a wavelength of 366 nm. On the other hand, the test using HPLC at wavelengths of 230 nm and 280 nm showed the presence of two dominant secondary metabolites contents: flavonoid and phenolic. IC50 (ppm) of Bauhinia semibifida (6.6247), Penawa Root (5.0124) and Cnestis palala (5.9968) were much lower than IC50 of mangosteens rind (41.7675), vitamin C (6.6612) and Stimuno drug (8.333). This antioxidant analysis has not been reported previously. This proof contributed greatly to uncovering potentially native natural resources as an indigenous Indonesian drug which is expected to decrease dependence on imported drugs especially imunomodulator, antihypertensive, antidiabet etc. This research would be beneficial and excellent manifestation for the development of natural antioxidant-based medicines from traditional knowledge of Indonesias local ethnicities.]]>
<![CDATA[The Gonad Maturity of Female Osteochillus vittatus in the presence of Ascorbic Acid ]]>
<![CDATA[Setyaningrum, Nuning]]>;
<![CDATA[Sugiharto, Sugiharto]]>;
<![CDATA[Hidayah, Hexa Apriliana]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i2.9848
<![CDATA[The fishs reproduction status is affected by both fed compositions and vitamins intake lead to determine improvement of eggs quality as well as fish production. The presence of Ascorbic Acid (AA) in the cultivation ponds, might accelerate females gonad maturation and so rematuration. The research aimed to determine: (1) Level gonad maturity of supplementation Ascorbic Acid; (2) Oocyts diameter; (3) Larvae survival rate. The research used experimental methods.The method was a completely randomized design (CRD) of 4 treatments and 4 replications. Treatments were supplementation of AA at different dosages of 0, 600, 1200 and 1800 mg/kg fish-fed ration.The gonad maturity level was analysed descriptively and oocyte diameter and larvae survival rate data were analysed by ANOVA. The result showed that supplementation of AA at a dosage of 1200 mg / kg fish-fed ration accelerated the process of gonad maturity, development of oocyt diameter and larvae survival rate as well as the viability of O. vittatus larvae at 90 rearing days. In this case, level IV gonad maturity was reached at 90 days which characterized by completed vitelogenesis process and oocyte diameter of 1.1 mm. Thus, this study is useful for aquaculture science by providing information on utilization of AA as food supplement in fish culture and also for fish farmer who wish to accelerate O. vittatus reproduction.]]>
<![CDATA[Genetic Variation in Cytochrome b-Hinf1 and -Alu1 Gene Correlated to Body Size in Soang Gourami (Osphronemus goramy) from Single Spawning ]]>
<![CDATA[Kusbiyanto, Kusbiyanto]]>;
<![CDATA[Soedibja, Petrus Hary Tjahja]]>;
<![CDATA[Nuryanto, Agus]]>;
<![CDATA[Qonita, Nael Huda]]>;
<![CDATA[Pramono, Hendro]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i2.9301
<![CDATA[Soang gourami fingerling shows variable body sizes eventhough resulted from single spawning. Differences in body sizes among individuals is assumed to be correlated to their genetic component which can be studied using cytochrome b gene PCR-RFLP marker. This study aimed to determine specific PCR-RFLP marker among different sizes of soang gourami collected from single spawning. Genomic DNA was isolated using Chelex method. Cytochrome b gene were amplified and digested using four restriction enzymes. Specific markers were analyzed descriptivelly based on DNA band pattern appear in agarose gel. The result showed that PCR-RFLP markers of Cytochrome b-HinfI of 315 bp, and 210 bp, and also Cytochrome b-AluI of 334 bp and 189 bp are specific markers for large individuals, whereas small individuals are characterized by having Cytochrome b- HinfI 366 bp, and 159 bp and Cytochrome b-AluI 525 bp fragments. It is observed that genetic variation of Cytochrome b-HinfI and -AluI markers are possitively correlated to body size in soang gourami fingerling. Therefore, both cytochrome b-HinfI and -AluI gene can be reffered as specific markers to differentiate among different sizes of soang gourami strain fingerling from single spawning. This result proved that genetic divergences among individuals can be related with certain quantitative characters, such size related. Therefore our study can contribute on fisheries development, especially by providing new technique for fingerling selection to obtain high quality fingerling and also provide new insight the application of molecular technique in fisheries.]]>
<![CDATA[Effect of Temperature Shock on the Triploidization Success of Seurukan Fish (Osteochilus vittatus) ]]>
<![CDATA[Eriani, Kartini]]>;
<![CDATA[Syahrin, Alfis]]>;
<![CDATA[Muchlisin, Zainal Abidin]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i2.8680
<![CDATA[Seurukan fish (Osteochilus vittatus) has many advantages, besides the fish also has disadvantages which are the slow growth, so the temperature shock of triploidization technique was expected to solve the problem. The objective of the present study was to obtain an effective temperature to increase of triploidization success of seurukan fish (Osteochilus vittatus). The experimental method and completely randomized design model were used in this study. Five levels of temperature shocks at three replicates were tasted: 4? (cold), 6? (cold), 28? (normal), 35? (heat) and 37? (heat). The sperms and eggs were fertilized in the plastic jar then a total 100 of fertilized eggs (zygotes) were taken randomly 3 minutes after fertilization and soaked in respective temperature for 90 seconds, and then incubated in incubation jars at the water temperature of 28-29?. The results showed that the temperature shock gave the significant effect on the hatching and the success of triploidization success (P?0.05), but did not give the significant effect the fertility and survival rates (P>0.05). The triploid fish can be achieved using cold and heat shock, but the higher triploid fish was recorded at 37? was the best temperature recommended for triploidization of Seurukan fish.]]>
<![CDATA[The Effect of Physical Activity agains the Telomere Length in the Leukocytes Cells of KONI Athletes ]]>
<![CDATA[Purwaningsih, Endang]]>;
<![CDATA[Djannatun, Titiek]]>;
<![CDATA[Widayanti, Etty]]>;
<![CDATA[Suciati, Yulia]]>;
<![CDATA[Zulhamidah, Yenni]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v9i2.6207
<![CDATA[Telomeres are strands of non coding DNA at the ends of chromosomes that have the primary function to protect DNA from damage and maintain chromosomal stability. Physical exercise will increase the antioxidant activity can increase telomere proteins, lengthen telomeres and or protein networks associated with telomere so that the telomere remains long, or stopping telomere shortening. Telomere length was also associated with age. The purpose of the research was to determine telomere length of leukocyte cells in the KONI (Indonesian National Sports Committee) athletes in Jakarta. The research method is descriptive, by measuring telomere length using quantitative PCR on leukocyte cells. Samples are KONI athletes from several sports, including men and women athletes, with ages between 15-20 years. Used a control group (not athletes) is students of the Faculty of Medicine, University of YARSI. The results showed that there was no significant difference (p> 0.05) between telomere length group of athletes with the control group in both sexes. Similarly, telomere length between athlete male with female athletes also showed no significant difference (p> 0.05). It was concluded that physical exercise in athletes KONI at the age of 15- 20 years had no effect on telomere length in leukocytes. The results of this study provide information about the telomere length in Indonesian athletes at an early age.]]>
