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Jurnal Natur Indonesia

JN
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Published by Universitas Riau

ISSN : 14109379 EISSN : 25030345 DOI : http://dx.doi.org/10.31258/jnat

Core Subject : Science, Agriculture, Engineering,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry

JURNAL NATUR INDONESIA terbit sejak tahun 1998, merupakan jurnal ilmu sains yang menyajikan artikel mengenai hasil penelitian, pemikiran dan pandangan dari peneliti dan pakar dalam bidang biosains (ilmu dasar), meliputi biologi, fisika, kimia dan matematika. Jurnal Natur Indonesia melibatkan mitra bestari yang menelaah setiap artikel sesuai dengan bidang ilmunya. Nama dan asal institusi mitra bestari tersebut tercantum pada halaman bagian normor 2 dari setiap volume penerbitan. Jurnal ini diterbitkan setahun dua kali, pada bulan April dan Oktober.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 242 Documents

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Pengaruh Kolonisasi Trichoderma spp. pada Akar Bibit Pisang terhadap Perkembangan Penyakit Layu Fusarium (Fusarium oxysporum f. sp. cubense) Nurbailis, Nurbailis; Martinius, Martinius
Jurnal Natur Indonesia Vol 13, No 3 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

The purpose of the research was to obtain the superior Trichoderma that had ability to colonize root with the resultbeing effective to supress Fusarium wilt desease and promote banana seedling growth. This experiment consistedof 2 factors and 4 replications. The first factor was Trichoderma spp. namely : A. Trichoderma koningii strain S6sh(TK-S6sh), B Trichoderma viride strain T1sk (TV-T1sk) and Trichoderma harzianum strain S10sh (TH-S10sh). Thesecond factor was the kind of banana namely a.Cavendis, b. Barangan and c. Kepok. The observation werecolonization ability, Fusarium wilt desease development and the banana seedling growth. The result showed thatTV-T1sk was the best spesies to colonize all banana seedling root. The highest colonization in Barangan bananaseedling root reached 80%. Trichoderma colonization in banana seedling root could suppress Fusarium wilt diseasedevelopment and increase banana seedling weight. Higher ability of Trichoderma to colonize banana seedling rootcaused lower disease incidence of Fusarium wilt and greater biomass of banana seedling. Interaction betweenTV-T1Sk and Barangan banana was the best in colonization, so they were effective to suppress Fusarium wiltdesease and increase banana seedling biomass.

Pengaruh Sitokinin Eksogen dan Sukrosa terhadap Produksi Biomassa dan Alkaloid Canthinone di dalam Kultur Suspensi Sel Pasak Bumi (Eurycoma longifolia Jack.) Mahmud Siregar, Luthfi Aziz
Jurnal Natur Indonesia Vol 12, No 2 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

The effect of addition cytokinins and modification of sucrose concentration on growth and alkaloid canthinoneproduction in cell suspension cultures of Eurycoma longifilia Jack were studied. The additions of cytokines, BAand kinetin, show effect on the production of biomass and alkaloid in cell suspension of E. longifilia Jack. Theoptimum totals of two-alkaloids were obtained on addition 4.44 μM BAP and without kinetin, respectively. Theaddition of 4.44 μM BA (6-benzyladenine) into TAM medium stimulated increased total of 9-hydroxycanthine-6-one,but decreased total of 9-methoxycanthin-6-one. While the addition of 2.32 - 9.29 μM kinetin (6-furfurylaminopurine)into TAM medium decreased total of two alkaloids (from 0.582 mg to 0.461 - 0.257 mg per 25 ml medium). Whensucrose concentration in TAM medium was increased from 3% to 5%, production of biomass would increase from0.374 g to 0.585 g dry weight per 25 ml medium. While total of two-alkaloids increase from 0.328 mg to 0.441 mgper 25 ml medium when concentration of sucrose in TAM medium was increased from 3% to 4% sucrose.

Karakterisasi Bakteri Asam Laktat Proteolitik pada Bekasam Wikandari, Prima Retno; Suparmo, Suparmo; Marsono, Yustinus; Rahayu, Endang Sutriswati
Jurnal Natur Indonesia Vol 14, No 1 (2012)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Bekasam is a traditional fermented fish from Indonesia. It is made from fish in which the gills and gut had been discarded, washed, and mixed with salt, after two days, it was covered with rice and salt, and then fermented for 5-7 days at room temperature. Bekasam is perceived to have antihypertensive activity, which is though to be the activity of bioactive peptides, the product of protein degradation during the bekasam fermentation. The research was aim to isolate the indigenous lactic acid bacteria that produce strong proteolytic capabilities. The lactic acid bacteria were collected from 3 kinds of bekasam (tilapia, milkfish, and tuna bekasam). Isolation of the lactic acid bacteria was done on agar medium containing MRS + CaCO3, and the screening of the proteolytic bacteria was done by growing the isolated culture on skim agar. The colonies that produce clear zone were identified as proteolytic lactic acid bacteria. Some of 180 isolated strains of acid producing bacteria that produce clear zone on MRS agar were found 150 strains of lactic acid bacteria, and 84 strains showed their proteolytic activity. The later were identified morphologically and biochemical as Lactobacillus, Pediococcus and Leuconostoc genera. Selection was further conducted based on the height of the proteolytic and homofermentative fermentation activities. Upon species identification using API CH 50 kit, the selected strains belong to species of Lactobacillus plantarum, Lactobacillus pentosus, and Pediococcus pentosaseus.

Retrieved Optical Thickness for Analyzing The Aerosol and Cloud Properties Using Lidar Remote Sensing Dewang, Syamsir
Jurnal Natur Indonesia Vol 13, No 1 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

The lidar remote sensing is the one important application to observe the aerosol and cloud of the atmosphere. Themicropulse lidar (MPL) return signals were studied in the tropical area. In this investigation, the single scatteringis analyzed by the physical properties of aerosol and cloud. The signal simulation of the single scattering predictsthe maximum optical thickness by saturation. It was observed that saturation optical thickness from the lidarsignal depends on the variation of extinction coefficient. This simulation is compared by the optical thicknessestimation from the lidar data. The MPL data (at wavelength of 523 nm) was determined, and the sky radiometer (atwavelength 500 nm) was used as reference data. The maximum optical thickness of lidar was 2.6 at night time,and the maximum optical depth of lidar and sky radiometer data on the same day were 2.25 and 1.7, respectively.

Analisis Asam Lemak Omega-3 dari Minyak Kepala Ikan Sunglir (Elagatis bipinnulata) melalui Esterifikasi Enzimatik Handayani, Sri Seno; Gunawan, Erin Ryantin; Kurniawati, Lely; Murniati, Murniati; Budiarto, Lalu Haris
Jurnal Natur Indonesia Vol 15, No 2 (2013)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Omega-3 fatty acid is the essential fatty acid and important for human health. Omega-3 fatty acid is also really neededby pregnant and lactating mothers and also children to prevent the malnutrition. Omega-3 fatty acid is also reallyneeded by pregnant and lactating mothers, and children to prevent the malnutrition. The omega-3 fatty acid is commonlyfound in some fish like salmon, tuna, hering, and mackarel. However, those fish are expensive, so alternativesources relatively cheaper fish. One of fish that is low in price and high in production in Nusa Tenggara Barat is Sunglir(Elagatis bipinnulata ). The purpose of this research was to determine free fatty acids content, type of omega-3 fattyacids, and their compositions in head fish oil. Head of sunglir fish was used as a sample because the head was usuallynot consumed. Sunglir fish was obtained from the Ampenan beach (NTB) with a size of 20–30 cm. The extraction methodused in this study was soxhletation with n-hexane solvent and analyzed with Gas Chromatografi (GC), acid ethyl estersfrom the esterification enzymatic of fish oil. Result revealed that the fish oil contained 84% free fatty acid and 0.85%linolenic acid (ALA), 2.80% eicosatrienoic acid (ETA), 0.73% eicosapentaenoic acid (EPA) and 2.41% docosahexaenoicacid (DHA). Saponification and iodine number of head fish oil is 248.24 mg KOH/g oil and 227.16 g Iod/100 g oil.

Isolasi dan Identifikasi Bakteri Asam Laktat Proteolitik dari Susu Kedelai yang Terfermentasi Spontan Yusmarini, Yusmarini; Indrati, Retno; Utami, Tyas; Marsono, Yustinus
Jurnal Natur Indonesia Vol 12, No 1 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Lactic acid bacteria is a group of bacteria with proteolytic activities enambling to grow on protein rich substratesuch as soymilk. This research was aim to isolate and identify lactic acid bacteria with have proteolytic activityfrom spontaneous fermented soy milk. Sixteen isolates out of 26 colonies isolated from fermented soymilk arepresumed as lactic acid bacteria. Among these 16 isolates, only 3 of them showed proteolytic activity. These threeisolates were further identify morphologically and only two isolates identified as Lactic Acid Bacteria, namelyR.1.3.2 and R.11.1.2. The ability of these isolates to produce acid and protease were observed. The results showedthat isolate R 1.2.3 higher ability in producing protease.

Kemampuan Kitinase Streptomyces RKt5 sebagai Antijamur terhadap Patogen Fusarium oxysporum Yurnaliza, Yurnaliza; Margino, Sebastian; Sembiring, Langkah
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

The purpose of the reasearch is to determine of antifungal activity from chitinase from Streptomyces RKt5 to inhibite growth of Fusariumoxysporum. The chitinase of Streptomyces RKt5 produced in liquid chitin medium with optimum conditions (inoculum concentration, pHand incubation time) and then partially purified with ammonium sulphate. The enzyme products were tested the antifungal activity againstF.oxysporum. The results showed that mycelial growth of F.oxysporum can be inhibited by Streptomyces RKt 5 in dual culture test. Thepartial purified chitinase enzyme couldn’t inhibit the fungal growth. But if the mycellium fragmented, the enzyme could degrade the fungalcell wall in incubation time. The frequency of fungal cell wall lysis and levels of N-acetylglucosamine released that have been increasingalong with the length of incubation time.

Efek Hepatoprotektif Ekstrak Buah Merah (Pandanus conoideus Lam.) pada Hati Mencit Jantan Galur Swiss induksi dengan CCl4 Nugraha, Ari Satia; Hadi, Ninisita Sri; Siwi, Sri Untari
Jurnal Natur Indonesia Vol 11, No 1 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

A research on red fruit (Pandanus conoideus Lam.) has been conducted to determine its hepatoprotective effect.This research was concern on three group of treatments, a blank treatment (water), a standard drug (curcuminecontaining) and red fruit extract treatment group. The liver destruction was induced by CCl 4. The hepatoprotectiveeffect was illustrated by SGOT – SGPT level of activity and percentage of cell destruction obtained from histopatogolicanalysis. Compared to the blank group, which had level of SGOT-SGPT activity as 38224,40  2,92 U/L and SGPT of24128,00  5,22 U/L, the red fruit treatment group showed a lower SGOT – SGPT activity (20112,4  2,68 U/L and18923,0  2,77 U/L, respectively); while the standard drug treatment group showed level of SGOT and SGPT activityas 29732,4  1,85 U/L and 20640,8  3,78 U/L, respectively. The histopatologic evaluation also illustrated similaritythat the red fruit treatment group occupied the lowest percentage of hepatocyte destruction shown as percentageof cell degeneration and cell necrosis of 28,3% and 31,7%, respectively. The standard drug treatment showed78,3% of destruction based on degenerative cell destruction and 88,3% based on cell necrosis. Almost 100% of celldestruction was shown in the blank group. Based on these result, the red fruit extract possessed a liver cellprotection activity against cell destruction caused by CCl4 exposure and even more active than a standard drug.

Kuersetin dari Daun Erythrina poeppigiana (leguminosae) Herlina, Tati; Supratman, Unang
Jurnal Natur Indonesia Vol 17, No 1 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Erythrina plants known plants “dadap” is a higher plant that grows in tropical and subtropical regions. E. poeppigiana plants was a source of secondary metabolites, which contain flavonoids. This study aims to isolate the flavonoid compounds from the leaves of E. poeppigiana through the stages of extraction, fraction, separation and purification. E. poeppigiana leaves powder (2.5 kg) was extracted with methanol and partitioned with n-hexane and ethyl acetate. Furthermore, the separation of ethyl acetate of E. poeppigiana leaves fraction using a combination of column chromatographic was obtained pure compound (5 mg) in the form of a yellow amorphous solid. The chemical structure of pure compound was based on the data spectroscopy (MS, UV, IR, 1H-NMR and 13C-NMR) and identified as the compound 3,3 ‘, 4’, 5,7-pentahidroksiflavon or known as quercetin.

Aquatic Plants for Acid Mine Drainage Remediation in Simulated Wetland Systems Munawar, Ali; Leitu, Farkhruddin Okte; Bustamam, Hendri
Jurnal Natur Indonesia Vol 13, No 3 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Aquatic plant is an important component of a constructed wetland system for treating acid mine drainage (AMD).This study was conducted to investigate the remediation effects of planting three aquatic plants species on AMDquality in simulated wetland systems. Simulated wetland systems were constructed using 10-L plastic containersas growth media comprising mixed-organic substrates and aquatic plant species as planting treatments. Thetreatments involved individual plantings with Fimbristilys hispidula (Vahl) Konth, Mariscus compactus (Retz) Druce,and Typha angustifolia L., and mixed-planting with a combined three-plant species. As the control was the unplantedmedia. The plants were continuously flooded with very acidic AMD collected from a mine pit in PT TambangBatubara Bukit Asam, South Sumatra. During the experiment, the acidity (pH), oxidation reduction potential (Eh),and electrical conductivity (EC) of the flooding AMD were measured after 24 hours of the flooding, and thenbiweekly until the plants entered their reproductive stage. To estimate Fe removed by plants, AMD samples weretaken from both planted and unplanted systems for total dissolved Fe analyses. The data revealed some remediationeffects of planting aquatic plants on AMD in the wetland treatment systems. The presence of plants in the wetlandsystem appeared to induce oxygen diffusion to surrounding roots, which might result in Fe precipitation on rootsurface. Although no differences among planting treatments, Fe removals by plants highly correlated (R2=0.92)with the production of plant biomass.

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Editorial Address Gedung LPPM Universitas Riau Jl. HR Subrantas Km 12,5 Pekanbaru 28293

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Titania T Nugroho

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titania.nugroho@lecturer.unri.ac.id

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Gedung LPPM Universitas Riau Jl. HR Subrantas Km 12,5 Pekanbaru 28293

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