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Jurnal Natur Indonesia

JN
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Published by Universitas Riau

ISSN : 14109379 EISSN : 25030345 DOI : http://dx.doi.org/10.31258/jnat

Core Subject : Science, Agriculture, Engineering,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry

JURNAL NATUR INDONESIA terbit sejak tahun 1998, merupakan jurnal ilmu sains yang menyajikan artikel mengenai hasil penelitian, pemikiran dan pandangan dari peneliti dan pakar dalam bidang biosains (ilmu dasar), meliputi biologi, fisika, kimia dan matematika. Jurnal Natur Indonesia melibatkan mitra bestari yang menelaah setiap artikel sesuai dengan bidang ilmunya. Nama dan asal institusi mitra bestari tersebut tercantum pada halaman bagian normor 2 dari setiap volume penerbitan. Jurnal ini diterbitkan setahun dua kali, pada bulan April dan Oktober.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 242 Documents

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Purifikasi Parsial Enzim Ekstraseluler (Anoxybacillus sp.) yang Diisolasi dari Sumber Air Panas Bukit Kili Solok serta Aplikasinya untuk Menghidrolisis Limbah Berserat Octarya, Zona; Syukur, Sumaryati; Purwati, Endang
Jurnal Natur Indonesia Vol 15, No 2 (2013)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Termostable enzyme from thermophilic bacteria is very potential to improve technical enzyme in industry which used hightemperature. High water temperature exerts selection pressure on microbial species leading to specific flora that survivesand tolerates heat stress. The relative isolation and unique physical properties of Bukit Kili Ketek Hot Springs in Solok,West Sumatera may yield unique thermophiles. The generation of extracellular enzymatic bacterial is highly desirable forproduction of hydrolitic enzymes, which are useful in various industrial application and in animal feeds. This study,conducted to purify extracellular enzymes from thermophilic bacteria (Anoxybacillus sp.). This bacteria was isolated inBukit Kili Ketek Hot Springs, and after identified by analysis of 16S rRNA gene, 97% of similiarty with Anoxybacillus sp.was of obtained. The temperature of the hot waters was 52°C and the pH was 8. Extracellular and hydrolytic enzymeproduction were screened by qualitative SDS-PAGE method. SDS-PAGE analysis gave protein bands at ±110 kDa, ±80 kDa,±60 kDa, 50 kDa, 25 kDa, and ±10 kDa, respectively. Extracellular enzymes were used to degrade cellulose waste. Thecellulose activity for degradation of baggasse and pineapple pulp was 0,451 IU/mL and 0,310 IU/mL at 50°C and pH 6.

Isolasi, Pemurnian dan Karakterisasi Lipase Bakteri Hasil Skrining dari Tanah Tempat Pembuangan Akhir (TPA) Gunung Tugel Banyumas Zusfahair, Zusfahair; Setyaningtyas, Tien; Fatoni, Amin
Jurnal Natur Indonesia Vol 12, No 2 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

A bacterial lipase producer was isolated from garbage dump soil and was identified its genus. Lipase was extractedaccording to production time optimized, purified using ammonium sulfate fractionation and gel chromatograph.Determination of enzyme characteristic studied were influence of pH, temperature, various metals to lipaseactivity. The result of this research shows that the genus of isolated bacteria which produced lipase wasAcinetobacter sp., the lipase optimum production time is about 18 hours with the activity is about 115 unit/mL. Thehighest activity of lipase fractionation using ammonium sulfate is about 45% and the highest activity of purifyingwith filtration gel chromatograph column using Sephadex G-150 at 24 th fraction. Lipase from crude extract andpurifying product at this fraction has optimum pH 6 and optimum temperature is about 40 oC. Lipase to be classifiedas metalloenzyme that shows with decreasing the activity after added the EDTA. Metals ion, such as Cu 2+ and Zn2+were inhibited the lipase activity. Ca 2+ ion could increase lipase crude extract activity but inhibited the activity oflipase purifying product. Hg2+ ion could increase the activity of lipase purifying product.

Sintesis Asam-Asam Lemak Hidroksamik dari Minyak Kelapa Menggunakan Lipase sebagai Katalis Suhendra, Dedy; Gunawan, Erin Ryantin
Jurnal Natur Indonesia Vol 14, No 1 (2012)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Fatty hydroxamic acids (FHAs) have been successfully synthesized from coconut oil by a one-step lipase catalyzed reaction. FHAs are theacids that are based on fatty acids. Their hydrophobicity can be use for some applications such as surfactant and metal ions extraction fromaqueous media. This paper describes enzymatic synthesis of FHAs from coconut oil by reacting hydroxylamine with the substrate catalyzedby a lipase. The lipase used was Lipozyme, a commercially lipase of Mucor meihe fixed on a polymer anion exchange resin. The use ofimmobilized lipase as the catalyst for the preparation reaction provides an easy isolation of the enzyme from the products and othercomponents in the reaction mixture. In addition, it also allows the reaction to be carried out under mild conditions, which reduces thereaction’s side products. The optimum preparation conditions obtained were as follows; the reaction temperature was 30 oC, the reactiontime was 30 h, the ratio of coconut oil : lipozyme (g : g) was 29.5, and the ratio of hydroxylamine : coconut oil (mmol : mmol) was 6. Thesolvent used was hexane. The purified products were characterized by qualitative test, such as FTIR spectroscopy and HPLC.

Terjadinya Isomerisasi dan Oksidasi - dan -Karoten Selama Proses Pengolahan CPO Kusumaningtyas, Reni Subawati; Martosupono, artanto
Jurnal Natur Indonesia Vol 11, No 1 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Isomerization and oxidation has been known as the major factors of the degradation of carotenoid compounds.The carotenoid compounds in oil palm fruit are dominated by - and -karoten. Result of this experiment showedthat the isomerization of the - and -karoten during the process productions of crude palm oil (CPO) indicated bythe reducing of trans ? and trans -caroten concentrations and followed by the increasing of cis - and cis ?-caroten concentrations. Isomerization promote to increasing the concentration of cis - and cis -carotenapproximately 40 ppm and 19 ppm on the sterilization process, then 79 ppm and 134 ppm on the extraction andclarification process of crude oil. On the other hand, the purification process just only increased the concentrationof cis ?-karoten about 19 ppm. The Oxidation of the - and -caroten caused to the increasing of lutein andzeaxantin as the degradation product. The - and -caroten oxidation can be increasing the amount of lutein andzeaxantin approximately 1 ppm and 5 ppm on the sterile fruit, 22 ppm and 14 ppm on the extract crude oil, 1 ppmand 3 ppm on purifying oil. The oxidation process of oil palm extract in the vacuum drier tank can be increased thelutein concentration about 6 ppm.

Pengaruh Ukuran Partikel Arang Sekam Padi Dan Waktu Refluks terhadap Kadar Abudan Daya Serap Karbon Sekam Padi Solihudin, Solihudin; Noviyanti, Atiek Rostika; Rahayu, Iman
Jurnal Natur Indonesia Vol 17, No 1 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

The highest content in rice husk is ash with its main component of silica. Silica in ash causes the low adsorption capacity. Activated carbon from rice husk can be obtained by lowering the ash content. The aim of this study is determine the effect of particle size of charcoal and reflux time the ash content using a solution of potassium carbonate. The activation of rice husk char was carried out by using reflux methods with a potassium carbonate solution. The resulted carbon was washed using hydrochloric acid tested using iodine and methylene blue to examine of its adsorbance. The particle size of rice husk and reflux time decrease ash content in rice husk. In general, husk with the size of 80 mesh can produce carbon with low ash content and high absorption using reflux time at least for 120 minutes. Rice husk size of 100 mesh refluxing with potassium carbonate for 150 minutes can reduce the ash content as 91.85% and adsorptivity of the iodine at 331 mg.g -1 .

Identification of Agronomic Traits of Centella asiatica (L.) Urban. Naturally Grown at Regions With Different Altitudes Halimi, Entis Sutisna
Jurnal Natur Indonesia Vol 13, No 3 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

The Centella asiatica is an important herbal plant in Indonesia. The research was done to describe severalagronomic traits that related to yield of the plants grown at regions with different altitudes. The research wascarried out at three different regions of highland, midland, and lowerland, each at >500m, 250-500m, and < 250mabove sea level. The research location was Pagar Alam, Musi Rawas, and Ogan Ilir, respectively. Research indicatedthat the agronomic traits of the plants grown at highland, midland, and lowerland, regions were about the same forgeneral appearance of leaf shape and flower color. The leaf size, number and length of stolone, number of rossets,length of rhizomes, and number of flower were varied depending on the region. The plants grown at highland andmidland region tend to have larger leaf size, shorter petiole and stolone, more number of rossets and stolone,longer rhizomes, and more number of flowers per plant, compared to the plants of lowerland region. This could beindication that Centella asiatica plants grown on midland, and highland were more productive than plants oflowerland region.

Suhu dan Waktu Inkubasi untuk Optimasi Kandungan Eksopolisakarida dan Fitohormon Inokulan Cair Azotobacter sp. LKM6 Hindersah, Reginawanti; Sudirja, Rija
Jurnal Natur Indonesia Vol 13, No 1 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Azotobacter inoculation could play an important role to enhance the effectiveness of bioremediation since bacterialexopolysachharides form a complex with heavy metal. So that metal mobility in soil and its uptake by plantsincreased. Azotobacter also produce phytohormone which induce roots growth and subsequently the uptake ofnutrients. The objective of this research was to obtain optimal incubation temperature and time in Azotobacter sp.LKM6 liquid inoculants production in the fermentor to maximize the synthesis of exopolysachharides andphytohormones. The experiment arranged in Completely Randomized Design consisted of two incubationtemperature (room temperature and 300C). At 24, 36, and 48 hours incubation, the concentration of EPS andphytohormone cytokinin and giberrelin were occurred. The experimental results were 1) the best temperature andincubation time to produce Azotobacter sp. LKM6 liquid for bioremediation of heavy metal-contaminated soil was30oC and 48 hours, and 2) inoculants production at 300C for 48 jam produce liquid inoculants containing 2.87mg L-1 exopolysachharides, 81.0 mg L-1 cytokinins and 18.7 mg L-1 giberrelin, and 13.12 x 108 cell ml-1.

Profil Plumbum (Pb) dan Cadmium (Cd) sebagai Kontaminan Dampak Penggunaan Agrokimia serta Remediasi Biji Kedelai Menggunakan Swelling Agent pada Khelasi dengan Asam Sitrat Priyadi, Sapto; Darmaji, Purnama; Santoso, Umar; Hastuti, Pudji
Jurnal Natur Indonesia Vol 15, No 1 (2013)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

This research were aimed to know the plumbum (Pb) and cadmium (Cd) profile on soybean seeds as contaminants of the impact use of agrochemistry material impact and to know of swelling agent of N,N-dimethylformamide (DMF) needs at certain level of pH in chelation with citric acid. The results showed that the content of Pb in soybean seeds at cultivation system agrochemistry materials i.e. 0.63 ppm, while cultivation system without agrochemistry materials i.e. 0.46 ppm. Cd content in soybean seeds at cultivation system agrochemistry materials i.e. 0.05 ppm and the cultivation system without agrochemistry materials i.e. 0.1 ppm. Acceptable daily intake in terms of Pb content in soybean after treatment increases to 2.93 times compared before treatment (11.3379 to 33.2099 g per body weight per day). Acceptable daily intake in terms of the content of the Cd in soybean after treatment increases to 5.26 times compared before treatment. The value of the bio-concentration factor which expressed an accumulation of Pb in soybean seeds on both cultivation is low system (BCF<250), while for Cd is a moderate to high level (1.000<BCF>250). Cd reduction on soybeans seeds for all chelation treatment was maximum (not detected-detection limits was 0.01 ppm), while the highest reduction of Pb occurs at swelling agent N, N-dimethylformamide 15 mL at pH 10.

Pengaruh CdCl2 terhadap Produksi Eksopolisakarida dan Daya Hidup Azotobacter Hindersah, Reginawanti; Arief, Dedeh Hudaya; Soemitro, Soetijoso; Gunarto, Lukman
Jurnal Natur Indonesia Vol 12, No 1 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

The contamination of toxic heavy metal Cadmium (Cd) in soils will be endanger the human health because it ismore available comparing to another toxic heavy metals. One method of Cd-contaminated soil bioremediation isusing exopolysachharide-producing bacteria Azotobacter. Exopolysachharides (EPS) can mobilize Cd through theformation of complex Cd-EPS which sequentially can increase the availability of Cd for plants uptake. A laboratoryexperiment has been done to study the EPS production and the viability of six Azotobacter isolates in the liquidculture containing 0.01, 0.1, and 1 mM CdCl2. The bacteria were cultured in liquid medium with and without CdCl2 for72 hours at room temperature. The EPS production was determined by gravimetric method after precipitationusing acetone and centrifugation at 7000 rpm. The result was that all of Azotobacter isolates produce EPS in thepresence of CdCl2. In the culture with 1 mM CdCl2, the density of Azotobacter sp. isolate BS3, LK5, LKM6 increasedsignificantly, and that of isolate LH16 decreased. No significant effect of CdCl2 on the density of isolate BS2 andLH15. This research suggested that some Azotobacter isolates were relatively resistence to the Cd and could bedeveloped as biological agents in Cd-contaminated soil bioremediation.

Kajian Awal Pemanfaatan Rumput Teki (Fimbristylis sp), Linggi (Penicum sp) dan Sianik (Carex sp) sebagai Serat Alami untuk Bahan Alat Penangkapan Ikan Nofrizal, Nofrizal; Ahmad, Muchtar; Syofyan, Irwandy; Habibie, Ied
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

This research was aimed to study about the potential of fiber structure teki grass (Fimbristylis sp), linggi grass (Penicum sp) and sianik grass(Carex sp) as natural fibre for fishing gear materials. The parameter of this research are; water content, elongation, breaking strength andhistology of the fiber. This research used the observation and experimental method. The observation method is used to know the fiberstructure and water content of teki grass, linggi grass and sianik grass. The experimental method was used to test the breaking strength andelongation of the objects in wet and dry condition. Based on the histology observation, teki grass has an epidermis layers as fiber structure.It consists of parenchyma tissue and collenchymas tissue.Fiber structure of linggi grass and sianik grass are similar with teki grass. Sianikgrass has the most excessive epidermis layer than others i.e. 5.57 gram. The average water content of teki grass is 1.03 gram, and linggi grassis 0.17 gram. Based on the breaking strength test, sianik grass is stronger than others with average strength 9.05 kgf in wet condition and9.75 kgf in dry condition. Meanwhile, breaking strength of the teki grass and linggi grass is 3.20 kgf and 4.16 kgf in wet condition and 4.30kgf and 5.25 kgf in dry condition. The average of the elongation of sianik grass is longer than others i.e. 24.50 mm in wet condition and18.75 mm in dry condition. The elongation of teki grass is 9.05 mm in wet condition and 11.10 in dry condition. The average of linggi grasselongation is 12.20 mm in wet condition and 12.90 mm in dry condition. Based on the breaking strength and the elongation test, the resultshowed that sianik grass is the most potential natural fiber as fishing gear material.

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Editorial Address Gedung LPPM Universitas Riau Jl. HR Subrantas Km 12,5 Pekanbaru 28293

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Titania T Nugroho

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titania.nugroho@lecturer.unri.ac.id

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