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All Journal Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Jurnal Ilmu Ternak Veteriner Indonesian Journal of Biotechnology Journal of Health (JoH) Jurnal Kedokteran Hewan

Asmarani Kusumawati

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Author-ID : 235070

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Veterinary Other

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ISOLATION AND CLONING OF ENV-TM SUBUNIT GENE OF JEMBRANA DISEASE VIRUS IN THE PROKARYOTIC EXPRESSION VECTOR pGEX-2T Asmarani Kusumawati; Ronny Martien; Soesanto Mangkoewidjojo; Joeannes Sri Widada
Jurnal Sain Veteriner Vol 21, No 2 (2003): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

CLONING OF THE GENE ENCODING SAGI OF LOCAL ISOLATE TOXOPLASMA GONDII IN ESCHERICHIA COLI DH5a Sri Hartati; Sri Widada; Sumartono .; Asmarani Kusumawati
Jurnal Sain Veteriner Vol 21, No 2 (2003): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Isolation-Amplification of ENV-TM Subunit Genes of Jembrana Disease Virus by a Single Step RT-PCR and Its Direct Cloning in PCR2.1-TOPO Plasmid Asmarani Kusumawati; Basuki Hidayat; B. Sardjono; Joannes Sri Widada
Buletin Peternakan Vol 27, No 1 (2003): Buletin Peternakan Vol. 27 (1) Februari 2003
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v27i1.1459

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CORRELATION OF PROGESTERONE AND CORTISOL PLASMA LEVELS BETWEEN OVULATED AND NON-OVULATED ETTAWA CROSSBREDS DOES Pudji Astuti; Diah Tri Widayati; Sunendar -; Kresno Suharto; Asmarani Kusumawati; Aris Junaidi
Jurnal Veteriner Vol 9 No 1 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.695 KB)

This experiment was conducted to determine the correlation of progesterone and cortisol levels in plasma between ovulated and non-ovulated Crossbreds Ettawa does. Eight does were used in this experiment and they were divided equally into 2 groups, i.e. group I consisting of 4 normal ovulated does with the average body weight of 37.5 ± 3.109 kg and group II consisting of 4 non-ovulated does with the average body weight of 28 ± 2.160 kg as group II. The estrus cycles of all does were synchronized using intravaginal device of controlled internal drug release (CIDR) accompanied by intra-muscular injection of prostaglandin F2 (PGF2) alpha. Immediately after the onset of estrus, blood samples were collected from jugular every 3 and 6 hours for 72 hours after onset of estrus. The concentration of cortisol and progesterone in plasma was assayed by enzyme linked immunosorbent assay (ELISA). The results showed that the average concentrations of cortisol in ovulated does (90.89±26.22 ng/mL) was higher than in non-ovulated does (42.70±37.18 ng/mL). Similarly, the concentrations of progesterone in ovulated does (0.098±0.0423 ng/mL) was higher than in non-ovulated does (0.093±0.056 ng/mL). It was evident that the change in progesterone level was closely associated with the change in cortisol level in plasma.

Diagnosis Toksoplasmosis Kongenital Berdasarkan Gen Surface Antigen-1 Toxoplama gondii Isolat Lokal Menggunakan Polymerase Chain Reaction (DIAGNOSIS OF CONGENITAL TOXOPLASMOSIS BASED ON SURFACE ANTIGEN -1 GENE OF LOCAL ISOLATE TOXOPLASMA GONDII USING POLY Dwi Priyowidodo; Sri Hartati; Asmarani Kusumawati; Joko Prastowo
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (122.315 KB)

Congenital toxoplasmosis has an important role in the transmission of toxoplasmosis in animals andhumans. Thus, a rapid and an accurate diagnostic method is needed. The aim of this study was to conductthe diagnosis technique of congenital toxoplasmosis in mice based on surface antigen-1 (SAG-1) gene oflocal isolates (IS-1) T. gondii using Polymerase Chain Reaction (PCR). A total of 15 pregnant mice Balb/C strain with the aged of eight weeks were used as experimental animal. Mice were intraperitoneallyinfected with 103tachizoit of T. gondii RH strain at day 9th of gestation. Amniotic fluids, blood, fetus, andplacenta then were collected at day 1, 2 , 3, 4 and 5 post infection. DNA was extracted from the abovesamples using PureLinkTM Genomic DNA Kit (Invitrogen, Life Technologies, US), and then amplified byusing specific primer based on SAG-1 gene of the local isolate T. gondii. This study shows that positivePCR result were seen in all samples of amniotic fluids at day 2 up to day 5 post infection. Fetus andplacenta samples also show positive PCR result at 3 up to day 5 post infection. Negative PCR result showsin blood samples, however. To conclude, PCR technique using SAG-1 gene of local isolates T. gondii as atarget gene, could be used to detect congenital toxoplasmosis from infected mouse samples such as, amnionfluids, fetus, and placenta. Further research was needed to apply the PCR method with SAG-1 gene of localisolate T. gondiion the human samples of congenital toxoplasmosis.

Physiological Response of Bligon Buck to Transportation: Relation to Level of Thyroid Hormone Pudji Astuti; Sarmin -; Asmarani Kusumawati; Claude Mona Airin; Hera Maheshwari; Luthfiralda Sjahfirdi
Jurnal Veteriner Vol 11 No 2 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (115.212 KB)

Transportated animals may subject to a variety of physical stimuli including metabolism, crowding,noise, handling, isolation, agitation, and extreme temperature .The aim of this study was to determine thechanges of serum T4 and T3 concentration, during animals transportation. Six adult Bligon buck with bodyweight ranging from 26-30 Kg were used in this study. Two weeks prior to the experiment, the animalswere given anthelmintic Albendazole to eliminate egg worm. All animals were fed standard diet in theirpen at 10% of their body weight per head daily and commercial concentrate also given everyday. Freshwater was provided ad libitum. All animals were transported around village for 16 hours starting from18.00 pm until 10.00 am in open small truck (3 x 2 m); eye contact each others would be possible. Bloodsamples were withdrawn from jugular vein using vacutainer tubes containing heparin into 1.5 mL glasstubes, then centrifuged at 500 g for 15 minutes. Plasma was collected to be stored at –20° C. The bloodwere collected every 4 hours from 8 hours before transportation ( at 10.00 am, 14.00 pm and 18.00 pm)until the time of arriving after transportation at 10.00 am. Plasma was harvested and stored at –20° Cuntil T3 and T4 concentrations were measured using ELISA method (enzyme linked immunosorbentassay) product DRG, Germany. The result showed that transportation of Bligon bucks for 16 hours havean affect on level of T4 only (P<0.05) and not for T3 concentration (P>0.05) due to physical stimuli such ascrowding, heat stress, noise, handling would be discarded so that the metabolic process was stable. Duringtransportation, decreasing of T4 levels indicated conversion of T4 to T3 to form active hormone.

Deteksi Bovine Herpesvirus-1 Secara Immunohistokimia pada Membran Korioallantois Telur Ayam Berembrio (IMMUNOHISTOCHEMISTRY DETECTION OF BOVINE HERPESVIRUS-1 IN CORIOALLANTOIC MEMBRANE OF CHICKEN EMBRYONATED EGG) Yuli Purwandari Kristianingrum; Charles Rangga Tabbu; Bambang Sutrisno; Sitarina Widyarini; Kurniasih .; Tri Untari; Asmarani Kusumawati
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.278 KB)

Infectious Bovine Rhinotracheitis (IBR) is caused by Bovine Herpes virus-1 in the cattle. The clinicalsigns demonstrate depression, anorexia, swelling of the vulva, redness of the vestibule, pustule and ulceron the vaginal mucosal. Based on previous research, IBR virus from the nasal swab could be grown inchorio-allantoic membrane of embryonated chicken eggs. This study aim was to confirm whether IBR virusin cattle could be grown in embryonated chicken eggs as a substitute for cell culture. A total of five nasalswab samples from the cows that were positive for IBR infection (diagnosed by Polymerase Chain Reactionand cell culture) were inoculated on the chorio-allantois membrane of embryonated chicken eggs.Observation of lesions performed at 3-5 days after inoculation. Re-inoculation (passage) was done threetimes. Pock characteristic lesions were observed on the corioallantoic membrane with the size of 5-7 mm,rounded shape, opaque edge, with necrosis in the central area. Furthermore, pock lesions were processedfor hematoxylin and eosin staining and immuno-histochemistry. The result of hematoxylin and eosinstaining showed that the formation of intranuclear inclusion bodies and vacuolization of the epithelial cellof membrane was observed. Immuno-histochemistry staining showed positive reaction for antibodiesagainst BHV-1 in the epithelial cells membrane. In conclusion, embryonated chicken eggs could be usedas a medium for detection of IBR.

Prevalensi dan Faktor Resiko Kawin Berulang pada Sapi Perah pada Tingkat Peternak (PREVALENCE AND RISK FACTORS OF REPEAT BREEDING IN DAIRY COWS AT THE FARMER LEVEL) Surya Agus Prihatno; Asmarani Kusumawati; Ni Wayan Kurniani Karja; Bambang Sumiarto
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (128.312 KB)

Repeat breeding is a syndrome which affected the reproduction and production efficacy of dairy cattle.The cause of this syndrome may be a herd problem or a variety of individual cow problems. This studyaimed to determine the prevalence and factors which contributed in the repeat breeding syndrome in dairycattle farms in Daerah Istimewa Yogyakarta (DIY). A total of 922 dairy cows which owned by 401 farmerswere used in this study. The cows had a normal estrus cycle, have had at least once calved, age 2.5-8 yearsold, in good health condition, raised traditionally by a farmer or communal system. Multistage and clustersampling method were used in this study. The total numbers of sample at each stage was determined by proportional, whereas the total numbers of farmer was determined using “sampel rambang” Data werecollected by interviewing farmers and direct observation at the farm. Data collected were farmer’s education,length of having farm, farm condition, distance from inseminator, farmer’s ability to detect estrus andestrus cycle, the farm and cow’s hygiene. Data were analyzed by descriptive statistics then followed by ChiSquare and Odds Ratio. The results showed that the prevalence of repeat breeder at the dairy farms was29.4%. Risk factors at the herd level were the most common i.e.: estrus detection once per-day (OR = 17.8);estrus detection twice per-day (OR = 7.9); unsightly sewer (OR = 10.0); soil floor of enclosure (OR = 2.6); andthe use of wells or rivers as source of water (OR = 2.0 and OR = 1.8, respectively.

Identifikasi Brucella abortus Isolat Lokal dengan Brucella abortus Strain Specific-Polymerase Chain Reaction (IDENTIFICATION OF LOCAL ISOLATES OF BRUCELLA ABORTUS USING BRUCELLA ABORTUS STRAIN SPECIFIC-POLYMERASE CHAIN REACTION ASSAY) Susan Maphilindawati Noor; Pratiwi Pujilestari Sudarmono; Asmarani Kusumawati; Anis Karuniawati
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.165 KB)

Brucella abortus Strain Specific-Polymerase Chain Reaction (BaSS-PCR) is a single multiplex PCRtechnique which able to identify and differentiate between Brucella abortus field strains (biovar 1, 2, and4), B. abortus vaccine strains, Brucella species, and non-Brucella species. In this study, BaSS-PCR wasapplied to identify local isolates of B. abortus in order to investigate the B. abortus strains that infectedcattle in Indonesia. Fifty local strains of B.abortus isolated from infected cattle in Java (Jakarta andBandung), South Sulawesi (Maros), East Nusa Tenggara (Kupang and Belu) were used in this study. TheDNA bands were observed by agarose gel in the presence of ethidium bromide. Identification was performedbased on the size and number of DNA products amplified by PCR from each isolates. The results showedthat the 50 isolates were of B. abortus field strains. This finding showed that the cause of bovine brucellosisin Indonesia is B. abortus field strains.

DETEKSI BRUCELOSIS PADA SUSU SAPI DENGAN UJI POLYMERASE CHAIN REACTION (PCR) Susan M. Noor; Pratiwi Sudharmono; Asmarani Kusumawati; Anis Karuniawati
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mendeteksi brucelosis pada sampel susu sapi dengan uji polymerase chain reaction (PCR) dan membandingkan tingkatsensitivitas dan spesifisitasnya dengan metode milk ring test (MRT). Sebanyak 24 sampel susu sapi yang dikoleksi secara aseptik dari lapang diuji PCR dan MRT. Hasil pengujian menunjukkan bahwa 79,17% (19/24) sampel susu positif brucelosis dengan uji PCR dan 83,33% (20/24) dengan uji MRT. Sensitivitas dan spesifisitas PCR mendeteksi brucelosis masing-masing sebesar 75 dan 100% dibandingkan dengan uji MRT.

Co-Authors A. Junaidi Agung Budiyanto ANIS KARUNIAWATI Aris Junaidi B. Sardjono Bambang Sumiarto Bambang Sutrisno Basuki Hidayat Charles Rangga Tabbu Claude Mona Airin D. Tri Widayati Diah Tri Widayati Dini Wahyu Kartika Sari Dwi Priyowidodo Elly Widyarni Eka Purnamasari Hastari Wuryastuti HERA MAHESHWARI I Gusti Agung Komang Diafari Djuni Hartawan J.Sri Widada Joannes Sri Widada Joeannes Sri Widada Joko Prastowo K. Suharto Khairun Nissa Kresno Suharto Kurniasih . LUTHFIRALDA SJAHFIRDI Nafratilova Septiana Ni Wayan Kurniani Karja Penny Humaidah Hamid Pratiwi Pujilestari Sudarmono Pratiwi Sudharmono PUDJI ASTUTI Rarastoeti Pratiwi Rifia Tiara Fani Ronny Martien Rosalinda Petra Maria Tae Lake S. Sunendar Sarmin - Setyawan Budiharta Sitarina Widyarini Slamet Soebagyo Soesanto Mangkoewidjojo Sri Hartati Sri Suryatmiati Prihandani Sri Widada Sumartono . Sunendar - Surya Agus Prihatno Susan M Noor Susan M. Noor Susan Maphilindawati Noor Tenri Ashari Wanahari Tri Untari Yuli Purwandari Kristiangingrum

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