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All Journal Jurnal Fitopatologi Indonesia Agrotrop : Journal on Agriculture Science Journal of Agricultural Science and Biotechnology E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Jurnal Penelitian Tanaman Industri Jurnal Penelitian Tanaman Industri (Littri)
I Dewa Nyoman Nyana
Agricultural Biotechnology, Faculty Of Agriculture, Udayana University
Agriculture, Biological Sciences & Forestry Industrial & Manufacturing Engineering Medicine & Pharmacology

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Pengaruh Infeksi Beberapa Jenis Virus terhadap Penurunan Hasil pada Tanaman Cabai Rawit (Capsicum frutescens L.) I WAYAN SUKADA; I MADE SUDANA; I DEWA NYOMAN NYANA; GEDE SUASTIKA; KETUT SIADI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT Effect of Infection of Some Viruses to the Decresing Yield on Rawit Pepper (Capsicum frutescens L.) This study aims to identify the types of viruses that infect the plants of chili pepper ( Capsicum frutescens L. ) in Kerta village, and to know the influence of some kind of viruses infection on yield decline. To verify the type of virus is done by taking a sample of the pepper plant leaf showing symptoms of the virus that has been demonstrated through serological and molecular tests. The results of this study indicate that the mosaic symptomatic chilli pepper plants were infected by some kind of virus such as CMV, TMV, and ChiVMV. Chilli pepper shows asymptomatic yellow were infected by the PepYLCV and chlorosis were infected by Polerovirus. The result of fruit chili harvest were showed the higher yields on the healthy plants with average 16.01 tonnes/ha, while the chilli that showed chlorosis symptomps were 8.42 tonnes/ha, and yellow symptomps were 3.07 tonnes/ha. The lowest were in the plants that shows mosaic symptomp with average 2.52 tonnes/ha. The virus infection caused the losses of yield of 47.40% in chlorosis symptomatic plants, 80.82% in the yellow symptomatic plant and 84.25%, in the mosaic symptomatic plants compared to healthy plants. High yield loss in the mosaic symptomatic plants were caused by the highest infection on chiilli pepper that infected by some kind of viruses.   Keywords : Chili pepper , mosaic, chlorosis, and yellow
Pengaruh Dry Heat Treatment dengan Penundaan Waktu Tanam Terhadap Pertumbuhan dan Hasil Tanaman Cabai Rawit (Capsicum Frutescens L.) I KADE DARMAWAN; I DEWA NYOMAN NYANA; I KETUT SIADI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.1, Januari 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

The Effect of Dry Heat Treatment with Time Delay Planting on Plant Growth and Yield of Chili Pepper (Capsicum frutescens L.)Viral pathogens which cause disease is a major problem in reducing the production of chili pepper. The younger the plants are infected by the virus, the more severe symptoms of the disease caused. Based on this statement, it necessary to do a research that aims to determine the effect of Dry Heat Treatment with a delay time of planting on growth and yield of chili pepper. The research design used was a randomized block design with three treatments and nine replications. The treatments tested consists of Dry Heat Treatment with a delay time of planting (DHT + TT), Dry Heat Treatment (DHT), and control (NT). The growth variable and the yield that had been observed in these experiments were the plant height (cm), number of primary branches, leaf chlorophyll content, incidence of disease, and the yield (tons / ha). The results showed that Dry Heat Treatment with a delay time of planting (DHT + TT) provides better results in the cultivation of chili with 86.27 cm plant height increased by 106.83% compared with controls (NT), the number of branches 12.61 increased 41.53 % compared with controls (NT), and the yield 17.21 tons / ha increased by 256.87% compared with controls (NT).
Pengaruh Rhizobakteria terhadap Hasil dan Mutu Benih Kacang Tanah (Arachis hypogaea L.) NI NYOMAN TRISNA KENCANA DEWI; I DEWA NYOMAN NYANA; I GUSTI NGURAH RAKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.4, Oktober 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Effect of Rhizobacteria on the Results and Quality of Peanut Seeds (Arachis hypogaea L.) This research aims to know the kind of rhizobakteria that have a better capability in improving yield and quality of seeds. The research was carried out in September – December 2017. The research started from the time of planting seed quality test until after harvesting. This study used a Randomized Complate Block Design (RCBD). The treatments tested were four types of rhizobakteria and one control (without application of rhizobakteria). Observations were made to plant height, number of leaves, the content of chlorophyll, the filled poods per plant, contains the seed weight per Ha, seedling vigor, storage vigor, electrical conductivity and weight of 1000 of seed. The results showed that the four rhizobacteria, RB35 (undis 1), RB36 (undis 3), RB3 (Kara Benguk), and RB9 (lamtoro plant) were able to increase the yield and quality of seeds. Peanut seeds yield per hectare with rhizobacteria treatment was able to increase yield with a range of 66,49% - 128,35% compared with controls was 1.94 tons / ha. Rhizobakteria increased the weight of 1000 seeds with a range of 42,43% - 70,29% compared with controls of 279.24 g. Treatment of rhizobacteria increased storage vigor of seeds by a range of 5.29% - 9.52% compared with control. Moreover also has real against the peanut plant growth (plant height and the number of leaves), the content of chlorophyll, the number of pods containing seed quality (physical and physiological).
Identifikasi Virus Yang Berasosiasi Dengan Penyakit Mosaik, Kuning, Dan Klorosis Pada Tanaman Cabai Rawit (Capsicum Frutescens L.) I GUSTI NGURAH BAGUS PRANATA PUTRA; NI MADE PUSPAWATI; I DEWA NYOMAN NYANA; I KETUT SIADI; GEDE SUASTIKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.3, Juli 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT Identification of virus that associated with Mosaic, Yellow, and Chlorosis disease on Chili Pepper (Capsicum frutescens L) This study aims to identify the types of viruses associated with mosaic, yellow, and chlorosis that infect the plants of chili pepper ( Capsicum frutescens L. ) in Kerta village, Gianyar. The method used is the ELISA (Enzyme Linked Immunosorbent Assay) and molecular techniques through PCR (Polymerase Chain Reaction). Research activities include (1) Survey endemic locations of viral diseases in Kerta village, Gianyar (2) Collecting leaf of chilli peper that shown, mosaic, yellowing and chlorosis (3) serology test by ELISA (4) molecular detection through PCR. The results show the percentage average of pepper plants showing mosaic symptoms (52.13%), yellow (22.75%), chlorosis (5.45%) and healthy plant (19.67%). Serology test by ELISA technique showed that the mosaic disease induced by the triple virus that is TMV, ChiVMV, and CMV, whereas yellow symptoms infected by PepYLCV and chlorosis symptoms, infected by Polerovirus. RT-PCR technique successfully amplify the target DNA fragment size of 650 bp for Polerovirus and PCR successfully amplified the target DNA fragment size of 700 bp for PepYLCV in accordance with the specific primers were used. Keywords : Chili pepper, mosaic , yellow, chlorosis
Pengaruh Waktu Panen Terhadap Daya Simpan Benih Kacang Panjang (Vigna sinensis L.) I MADE BAGUS ANGGARA; I GUSTI NGURAH RAKA; I DEWA NYOMAN NYANA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.3, Juli 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Effect of Harvest Time on the Save of Long Bean Seeds (Vigna sinensis L.). Supervised Long bean plants have indeterminate growth type, ie vegetative growth is still active even though it has entered the generative growth phase. The indeterminate growth type causes the pods to form cook not at the same time. When harvesting long bean pods for quality seed production, it needs to be studied. The seeds before planting must undergo a process of storing both short and long term storage, so the seeds saving power that produced from the time of harvesting different pods also needs to be studied. This study used a completely randomized design (CRD) with three treatments when harvesting pods for seeds, namely physiological pre-cooking, harvesting at physiological ripe, and harvest after physiological cooking. The treatment was repeated nine times. The results showed that the treatment of harvest time had an effect on the shelf life of the beans. Harvest time when physiologically cooked has the longest shelf life compared to harvest time after or before physiological cooking. Seed quality when harvested physiologically during storage obtained the highest compared with the quality of seed yields before and after physiological cooking.
Pengaruh Penggunaan Jaring Berwarna Terhadap Kelimpahan Serangga Aphis gossypii pada Tanaman Cabai Rawit (Capsicum frutescens L.) NYOMAN AYU TRISNA ARI UTAMI; I NYOMAN WIJAYA; I KETUT SIADI; I DEWA NYOMAN NYANA; GEDE SUASTIKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.4, Oktober 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT Effect of Barier Colored Nets to Abundance of Aphis gossypii on Cayenne Pepper (Capsicum frutescens L.) Cayenne pepper (Capsicum frutescens L.) is a plant that has a high economic value and has a many of uses that as seasoning at household, as an ingredient in various food processing industries and the manufacture of pharmaceuticals. Currently chili productivity in Indonesia is still low therefore needs a national chili can not be optimally. One reason is the insect infestation of  A. gossypii are a double role as pests and disease vectors. The use of red and white netting barier around planting chili is one of the pest control management that is environmental friendly. The purpose of this study was to determine the role of red-and-white nets barier in protect the entry of A. gossypii in chilli planting area. There are three treatments were tested using a red netting, white netting and planting without the use of nets. The results showed that the use of red and white nets can reduce insect populations of A. gossypii on peppers planting area. Average of the highest populations of A. gossypii on treatment without a net, then the white netting treatment and lowest in the red, so that the use of red nets can be used to reduce the population of A. gossypii on chilli crop.   Keywords: Chilli pepper , A. gossypii, colored nets
Pengaruh Waktu Inokulasi terhadap Kejadian Penyakit Tular Benih Bean Common Mosaik Virus (BCMV) pada Tanaman Kacang Panjang (Vigna sinensis L.) NI PUTU EKA HANDAYANI; I MADE SUDANA; I DEWA NYOMAN NYANA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.2, April 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Influence of Inoculation Time Toward the Incidence of Bean Common Mosaic Virus (BCMV) Seed Borne Disease on Long Bean Plants (Vigna sinensis L.) This study aims to determine how far the influence of inoculation period of BCMV towards the incidence of seed borne diseases on long bean plants. This research activity includes planting of beans in the field, sampling on symptomatic plant mosaic; inoculation of BCMV mechanically, observation of symptoms, measurement of chlorophyll content, incidence of disease, calculation yields, percentage of germination test, percentage BCMV seed borne, ELISA serology test and data analysis. Design used was a randomized block design (RBD) with seven treatments and four replications.The results showed that long bean plants with 2 wap (weeks after planting) inoculation treatment indicates the variation of symptoms as diverse as heavy mosaic (Mb), malformation of leavs (Md), and dwarf (K). The chlorophyll content inoculation period 2 wap of 9.98 SPAD. Higher incidence of disease inoculation period 2 wap is 93%. The long bean plants that were infected by BCMV at 2 wap inoculation period resulted in yield average of 12.19 grams with the highest level of yield losses amounted to 95.52%. The grown seeds at 2 wap inoculation period had 0% germination.
The percentage of Bean Common Mosaic Virus (BCMV) carried by seeds and detection of virus position inside long beans (Vigna sinensis L.) seeds in Bali NI LUH PUTU CITRA INNOSENSIA; I GEDE RAI MAYA TEMAJA; I DEWA NYOMAN NYANA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.3, Juli 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

The percentage of Bean Common Mosaic Virus (BCMV) carried by seeds and detection of virus position inside long beans (Vigna sinensis L.) seeds in Bali Long bean (Vigna sinensis L.) is a horticulture crop with an essential economic value in Indonesia and Bali. Long beans productivity in Bali reached 6023 tons in 2013, and decreased to 5157 tons in 2014. Cases of disease that lowers the production of long beans in Bali were caused by Bean Common Mosaic Virus (BCMV) infection by 56.06%. BCMV can be brought by the seeds (seed-borne disease) and can infect long beans plants since the early stage of development. This research aims to evaluate the percentage of BCMV transmission by commercial long beans seeds, commonly used by farmers in Bali, and observe the location of BCMV inside the seeds. The morphological observation was used to evaluate the percentage of BCMV brought by the seeds by observing BCMV specific symptoms appear on two weeks old long beans seedlings. To observe the location of BCMV inside the long bean seeds, harvested seeds from an infected plant were separated to collect the cotyledon and embryo. The RT-PCR technique was used as a method in this research. The result showed the percentage of BCMV brought by long beans seeds used by farmers in Bali categorized as high. The percentage of BCMV brought by seeds from the highest to the lowest were; variety A 48.39%, variety B 46.66%, variety C 43.59%, variety D 37.83%, variety E 22.86%, and variety F 22.72%. The variation of the symptoms was mosaic vein banding, malformation, and dwarf plant with wrinkled leaves. The BCMV brought by long bean seeds was resides on the embryo.
Kisaran Inang Bean Common Mosaic Virus (Bcmv) Penyebab Penyakit Mosaik Pada Tanaman Kacang Panjang (Vigna sinensis L.) A. A. GEDE PUTRA ADHITYA; I GEDE RAI MAYA TEMAJA; NI NENGAH DARMIATI; I DEWA NYOMAN NYANA; GEDE SUASTIKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.4, Oktober 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT Host range of Bean Common Mosaic Virus (BCMV) causal agent of mosaic diseases on long yard bean  (Vigna sinensis L.) This objective of the study is order to find out the host range of BCMV and symptom variation. DAS-ELISA (Double Antibody Sandwich-Enzyme Link Immunosorbent Assay) menthod was used in this study. The study consisted of (1) Host range evaluation by mechanical inoculation, (2). Symptom observation on tested plants, (3) Serological test with DAS–ELISA. The result indicated that BCMV can infected Leguminoceae plants (peanuts, long yard beans, and beans); Solanaceae plant (tomatoes); Cucurbetaceae plant (cucumbers); Amaranthaceae plant (spinach); and Balsaminaceae plant (henna flowers). DAS-ELISA analysis shown that those plants were infected by BCMV. The symptoms variation were mosaic, vein banding,  wrinkled, and curved on commercial long yard beans (aura and panah merah varieties); wrinkled on commercial long yard beans (KPK and pusaka hijau varieties), cucumber and spinach; wrinkled and rolled on beans; wrinkled and curved upside on henna flowers.   Key word : bean common mosaic virus ,  long yard beans, DAS-ELISA
Deteksi Molekuler Cucumber Mosaic Virus (Cmv) pada Tanaman Gamal (Gliricidia Sepium) Sebagai Barier pada Pertanaman Cabai IDA BAGUS GDE PRANATAYANA; I GEDE RAI MAYA TEMAJA; KETUT YULIADHI; I DEWA NYOMAN NYANA; GEDE SUASTIKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT Molecular Detection of Cucumber Mosaic Virus (CMV) In Gliricidia Plant (Gliricidia sepium) as Barrier at Chili pepper Crop The aims of the study are in order to symptoms characteristic identity the CMV infection on gliricidia plant and symptoms characteristic the CMV infection on chili pepper plant, and order to presence identity the CMV on gliricidia plant and presence the CMV on chili papper plant at Kerta village base on molecular analysis. The serology method was used to detect CMV virus is DAS-ELISA (Double Antibody Sandwich-Enzyme Link Immunosorbent Assay) and RT-PCR (Reverse Transcription-Polymerase Chain Reaction) was used for molecular analysis. The study include 1) the observation of mosaic in field, 2) preservation of plant samples (chili pepper and gliricidia) infected with mosaic virus, 3) serology test with DAS-ELISA, 4) molecular detection with RT-PCR. The research proved that symptoms characteristic the CMV infection on gliricidia plant identical with symptoms characteristic the CMV infection on chili pepper plant and presence the CMV on gliricidia plant identical with presence the CMV on chili pepper plant. symptoms of mosaic characteristic in gliricidia and chili pepper found on the field among others, mosaic (striped), contract and roll with variations of symptoms that light green mosaic with chlorotic and dark green mosaic symptoms in gliricidia and chili pepper plants. DAS-ELISA test results showed gamal and chili pepper samples infected positive of Cucumber Mosaic Virus (CMV). RT-PCR technique successfully amplify the target DNA fragment size of 657 bp. Based on these studies, the farmers need to be aware of the existence of the plant used as a plant barrier on chilli crop, because gliricidia potential as alternative hosts of CMV.   Key word : Chili pepper, cucumber  mosaic virus, gliricidia, molecular
Co-Authors A. A. GEDE PUTRA ADHITYA ANAK AGUNG MADE ASTININGSIH AYU GEK MIRAH LESTIANINGRUM DWI OCKVIAN ANESTA GEDE SUASTIKA Gede Suastika Gede Suastika GEDE SUASTIKA GEDE SUASTIKA Gede Suastika Gede Suastika Gusti Ngurah Alit Susanta Wirya I Dewa Made Putra Wiratama I G. R. M. TEMAJA I GEDE PUTU DARMAWAN I Gede Rian Pramarta I GUSTI ALIT GUNADI I Gusti Ngurah Bagus I GUSTI NGURAH BAGUS PRANATA PUTRA I GUSTI NGURAH RAKA I KADE DARMAWAN I Ketut Siadi I MADE BAGUS ANGGARA I MADE SUDANA I MADE SUDANA I NYOMAN WIJAYA I WAYAN SUKADA IDA BAGUS GDE PRANATAYANA KADEK DWI UTAMA KETUT AYU YULIADHI Khamdan Khalimi M. Tinny Lestariningsih Made Mika Mega Astuthi Miftakhurohmah Miftakhurohmah NI KADEK VENIARI NI LUH MADE PRADNYAWATHI NI LUH PUTU CITRA INNOSENSIA Ni Made Puspawati NI NENGAH DARMIATI Ni Nengah Putri Adnyani NI NYOMAN ALIT PURWANINGSIH NI NYOMAN DIAH UTTARI NI NYOMAN TRISNA KENCANA DEWI NI PUTU EKA HANDAYANI NYOMAN AYU TRISNA ARI UTAMI Putu Sudiarta Rita Noveriza ROBERTO BOBHOPE ARUAN SEDYO HARTONO Tomohide Natsuaki TRI ASMIRA DAMAYANTI