Garuda - Garba Rujukan Digital

Uji Kemampuan Beberapa Isolat Rhizobakteria untuk Meningkatkan Pertumbuhan dan Hasil Kedelai (Glycine Max (L)Merill) AYU GEK MIRAH LESTIANINGRUM; I GUSTI NGURAH RAKA; I DEWA NYOMAN NYANA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.1, Januari 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (119.226 KB)

Test Capabilities some Isolate Rhizobacteria for Enhancing the Growth and Yield of Soybean (Glycine max L. Merill)This study aims to get the isolates of rhizobacteria that have a better ability to increase growth and yield of soybean. Randomized block design was used with 14 treatments, which 13 are treated using rhizobacteria isolates from different plants root and one without isolates of rhizobacteria. Each treatment was repeated three times. This research was conductedin the pots with observations of the growth and yield of soybean that includes; plant height, leaf number, number of branches, chlorophyll, the number of nodules, dry weight of the plant part above the ground and below the ground, the number of pods, number of seeds, seed weight, and predict the outcome of soybeans per hectare. The result showed that three isolates rhizobacteria who have better abilities compared to other isolates rhizobacteria to improve the growth and yield of soybean that isolates R53, R6, and R26, with its ability to improve; plant height, number of branches, number of leaves, chlorophyll, the number of nodules, the number of dry weight of the plant above ground or below ground, the number of pods, number of seeds, seed weight and better ability to increase soybean yield per hectare. In addition to these three isolates are also two isolates rhizobakteria which only has the ability to increase soybean yield that isolates R10 and R11. The five isolates rhizobakteria ( R53, R6, R26, R10, dan R11 ) can be regarded as rhizobateria were able to spur the growth and yield soy called PGPR bacteria .

Efektivitas Penggunaan Pupuk Hayati (Enterobacter cloacae) untuk Meningkatkan Hasil dan Mutu Benih Padi Varietas Cigeulis NI NYOMAN DIAH UTTARI; I DEWA NYOMAN NYANA; ANAK AGUNG MADE ASTININGSIH
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.5, No.1, Januari 2016
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.655 KB)

ABSTRACT Biological Fertilizer Usage Effectiveness (Enterobacter cloacae) to Increase Yield and Quality of Rice Seeds Varieties Cigeulis The purposes of this research were to find out the influence of biological fertilizer Egary which contains rhizobacteria Enterobacter cloacae related to the crop of Cigeulis rice, as well as to test the effectiveness of biological fertilizer to reduce the utilization of chemical fertilizer, and to obtain the quality of the seed production. The resullt indicated that the augmentation of biological fertilizer plus chemical fertilizer phonska and urea at 100 kg/ha each (Py) increased the production of the rice crop about 1.8 tons/ha or increased about 33.93% and the result of biological fertilizer plus chemical fertilizer phonska and urea at 200 kg/ha each (Pz) increased about 0.8 tons/ha or about 33.13% if compared with control. The research of Py treatment with biological fertilizer plus chemical fertilizer phonska and urea at 100 kg/ha each, produced Cigeulis rice crop variety for 6.3 tons/ha and improved the quality of the seeds both physical and physiological parameters, produced 1000 grain weight of seeds, germination and vigor highest power savings and has better endurance than Pz and control (30.28 g, 89.11%, and 85.89%, respectively). Keywords: Fertilizer, seeds, yield

Deteksi Cucumber Mosaic Virus (CMV) dan Chili Veinal Mottle Virus (ChiVMV) pada Gulma Commelina spp. di Pertanaman Cabai(Capsicum spp.) Melalui Teknik Uji Serologi dan Molekuler NI KADEK VENIARI; KETUT AYU YULIADHI; I DEWA NYOMAN NYANA; GEDE SUASTIKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.1, Januari 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.455 KB)

ABSTRACT Detection of Cucumber Mosaic Virus(CMV ) and Chilli Veinal Mottle Virus (ChiVMV) on Weed Commelina spp. In Cropping Chilli Pepper(Capsicumspp.) Through Serology and Molecular Test This study aims to determine the type of weed Commelina spp. which became an alternative host Cucumber mosaic virus (CMV) andChili veinal mottle virus (ChiVMV) in pepper, as well as the detection of CMV and ChiVMV which infects the weed Commelina spp. use molecular methods. DAS-ELISA (Double Antibody Sandwich-Enzyme Linked Immunosorbent Assay) and molecular techniques through RT-PCR (Reverse Transcription-Polymerase Chain Reaction) are used. Research activities include (1) Survey mosaic symptoms and disease incidence in the field; (2) Identification the type of weed that grows on pepper plants area; (3) Preservation of samples Commelina spp. weed and chili which virussymptomatic; (4) Test through DAS-ELISA serology; (5) Molecular detection by RT-PCR. The research proves that the weed Commelina spp. can be alternate hosts ChiVMV and CMV, also CMV and ChiVMV can infect weedsCommelina spp in chilicropping. DAS-ELISA test results show samples of the weed Commelina spp. and chili infected with Cucumber mosaic virus(CMV) and Chili veinal mottle virus (ChiVMV). RT-PCR technique successfully applyingDNA target fragment size of 657 bp and 900 bp for CMV and ChiVMV accordance with the specific primers used. Keyword :Chili pepper, CMV, ChiVMV, weeds commelina

Studi Hasil dan Kualitas Benih Padi P05 dengan Pemberian Pupuk Hayati (Enterobacter cloacae) DWI OCKVIAN ANESTA; I DEWA NYOMAN NYANA; ANAK AGUNG MADE ASTININGSIH
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.5, No.2, April 2016
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (170.075 KB)

The Study Result and the Quality of the Seed Rice P05 by AdministeringBiological Fertilizer (Enterobacter cloacae)The purposes of this research were to find out the influence of biological fertilizer Egary which contains rhizobacteria Enterobacter cloacae related to the crop of P05 rice, as well as to test the effectiveness of biological fertilizer to reduce the utilization of chemical fertilizer, and to obtain the quality of the seed production.The resullt indicated that the augmentation of biological fertilizer plus chemical fertilizer phonska and urea at 100 kg/ha each (Pb) increased the production of the rice crop about 1.0 tons/ha or increased about 38.46% and the result of biological fertilizer plus chemical fertilizer phonska and urea at 200 kg/ha each (Pc) increased about 0,7 tons/ha or about 33.99% if compared with control. The research of Pb treatment with biological fertilizer plus chemical fertilizer phonska and urea at 100 kg/ha each, produced P05 rice crop variety for 7.2 tons/ha and improved the quality of the seeds both physical and physiological parameters, produced 1000 grain weight of seeds, germination and vigor highest power savings and has better endurance than Pc and control (29.62 g, 87.64%, and 81.45%, respectively).

Aplikasi Rizobakteri Pantoea agglomerans untuk Meningkatkan Pertumbuhan dan Hasil Tanaman Jagung (Zea mays, L.) Varietas Hibrida BISI-2 I GUSTI NGURAH RAKA; KHAMDAN KHALIMI; I DEWA NYOMAN NYANA; I KETUT SIADI
Agrotrop : Journal on Agriculture Science Vol 2 No 1 (2012)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (106.33 KB)

This experiment was conducted in the village of Sibang Gede, District of Abiansemal, BadungRegency on November 2011 until February 2012. The research objective is to enhance the growth andyield of maize hybrid BISI-2 using rhizobacteria Pantoea agglomerans as PGPR in compostformulations. This study used a randomized block design (RBD) single factor with 5 treatments and 5replications. The treatments tested were P. agglomerans BS7a, P. agglomerans BS7b, P. agglomeransBS2a, and P. agglomerans BS5a. All treatments P. agglomerans formulated in the form of compostand given a dose of 50 g/hole. As a control dose of compost 50 g/hole without PGPR. The resultsshowed that the application of rhizobacteria P. agglomerans increased the maximum plant height, netassimilation rate, crop growth rate, chlorophyll content of leaves, fresh weight of roots per plant, ovendried weight of stover per plant, number of seeds per plant, cob length, cob diameter, seed weightmoisture content of 12% per plant and seed yield of 12% water content per hectare.

Efektivitas PGPR Formulasi Kompos Dalam Meningkatkan Ketahanan Tanaman Kedelai terhadap Soybean Stunt Virus I KETUT SIADI; KHAMDAN KHALIMI; I DEWA NYOMAN NYANA; I GUSTI NGURAH RAKA
Agrotrop : Journal on Agriculture Science Vol 7 No 2 (2017)
Publisher : Fakultas Pertanian Universitas Udayana

Effectiveness of PGPR Compost Formulation in Improving Soybean Plant Resistance to Soybean Stunt Virus. Soybean stunt virus (SSV) is one of important obstacles of soybean production in Indonesia. This virus causes the stunting on soybean plant and may cause the yield losses up to 71%. Eight isolates of plant growth promoting rhizobacteria (PGPR) were isolated and tested for their efficacy to control SSV on soybean. Those isolates are Paj, Pak2, Pa1, Pa3, Pa4, BT, and KT. Application of PGPR was done by soaking the seeds in PGPR solution prior to planting and application of PGPR in compost formulation. Virus concentration and disease incidence were determined using DAS-ELISA. Results of this study showed that application of PGPR in compost formulation suppressed disease incidence caused SSV. Disease incidence on treated plants ranged between 10% to 25%, while all of plant (100%) on un-treated plants were infected. Peroxidase activity on treated plants increased by 80.25% to 97.33% in comparison with un-treated plants. These results suggested that application of PGPR in compost formulation could increase the resistance of soybean against SSV. Hence, PGPR can be considered as one of measures to control SSV on soybean.

Produktivitas Benih Cabai Rawit Setelah Diperlakukan Dry Heat Treatment dan Penyimpanan I GUSTI NGURAH RAKA; I DEWA NYOMAN NYANA; NI LUH MADE PRADNYAWATHI
Agrotrop : Journal on Agriculture Science Vol 3 No 1 (2013)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (67.028 KB)

Productivity of Pepper Seeds Which are Treated Dry Heat Treatment and Storage Pepper plants (Capsicum frutescens L.) is one type of horticultural crops which is very high usage levels therefor, it is necessary to increase productivity dramatically. One effort to fulfil the need is preparing healthy seed with long shelf life . This study aims to determine the growth and yield of pepper plants whose seed was treated with dry heat treatment and storage. The experiment was conducted in Br . Marga Tengah, Kerta Village, Payangan District, Gianyar Regency, since May to October 2013. This study used a randomized block design (RBD) with two factors and four replications. The first factor is treated seeds by dry heat treatment at two levels i.e. given dry heat treatment at 70OC for 72 hours (D1) and without dry heat treatment (D0). The second factor is the storage of seeds with three levels, namely: non-stored seeds (T0), seed stored 2 months (T2) and the seeds stored for 4 months (T4). The results showed that there was no interaction between treatment with dry heat treatment and seed storage treatment of all variables of growth and yield of pepper plants. Dry heat treatment resulted the better growth and yield compared to non dry heat treatment. An increase in the number of fruit harvest as much as 33,43% and increased the weight of the fruit harvest per hectare as much as 33,79% on dry heat treatment compared with no dry heat treatment. Treatment of seed storage until the shelf life of 4 months did not affect the growth and yield of pepper plants.

Karakterisasi Biokimia Aeromonas hydrophila dan Potensinya Sebagai Pemacu Pertumbuhan Bibit Tanaman Melon Emas I KETUT SIADI; KHAMDAN KHALIMI; DEWA NYOMAN NYANA
Agrotrop : Journal on Agriculture Science Vol 4 No 1 (2014)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (48.651 KB)

Biochemical Characterization of Aeromonas hydrophila and Its Potential for Growth Stimulatorof Golden Melo Seedlings.The results showed that this research got 20 nitrogen fix bacteria isolate,However, Pg2 isolate could increase the growth of golden melo. The treatment with Pg2 isolatesignificantly increased the golden melo growth. All the plant growth parameters of treated plants weresignificantly higher than those of un-treated control plants (P<0.05). The leaf chlorophyll content, plantgrowth rate, and fresh and dry weights of plant were significantly higher than those of un-treatedcontrol plants. Isolates identified as Enterobacter cloacae PG2 percentage of 98.05% probability.

IDENTIFIKASI MOLEKULER CUCUMBER MOSAIC VIRUS (CMV) ASAL TANAMAN NILAM (Pogostemon cablin) / Molecular Identification of Cucumber Mosaic Virus Infecting Patchouli (Pogostemon cablin) Miftakhurohmah Miftakhurohmah; I Dewa Nyoman Nyana; Tri Asmira Damayanti; Rita Noveriza
Jurnal Penelitian Tanaman Industri Vol 23, No 1 (2017): Juni, 2017
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v23n1.2017.11-17

Mosaic disease on Indonesian patchouli associated with infection of TeMV, BBWV2, CymMV and CMV. TeMV, BBWV2 and CymMV has been identified molecularly, while CMV just was detected serologically. The objective of this study was to identify CMV from patchouli by molecular approach based on CP gene nucleotide sequence Leaf samples were collected from three mosaic symptomatic patchouli plants in greenhouse of Balittro. Leaf samples were extracted for the total nucleic acids (RNA + DNA). Nucleic acids were amplified using specific primer for CP gene of CMV by one step RT-PCR technique. The DNA of PCR product with the size of ~ 650 bp was directly sequenced and analyzed for its homology with sequences of CMV isolates extracted from Gene Bank. CMV CP gene from patchouli showed the highest of nucleotide and amino acid sequence similarities, 97,1 and 97,7% respectively, with um Japanese isolates. Phylogenetic tree analysis revealed that CMV from patchouli was closely related with um-Japanese isolate with 100% bootstrap value, and clustered with another CMV isolates in subgroup IB. Since the CMV subgroup I was more virulent than subgroup, it is necessary to increase the awareness of the CMV occurrence in another plant.Key words : homology analysis, phylogeny tree, nucleotides, amino acid AbstrakPenyakit mosaik pada tanaman nilam di Indonesia berasosiasi dengan infeksi Telosma mosaic virus (TeMV), Broad bean wilt virus 2 (BBWV2), Cymbidium mosaic virus (CymMV) dan Cucumber mosaic virus (CMV). TeMV, BBWV2 dan CymMV sudah diidentifikasi secara molekuler, sedangkan CMV baru terdeteksi secara serologi. Karakte risasi molekuler setiap virus diperlukan sebagai salah satu dasar pengambilan tindakan pengendalian. Penelitian ini bertujuan untuk mengidentifikasi CMV asal tanaman nilam secara molekuler berdasarkan sekuen nukleotida gen CP. Tiga sampel daun nilam bergejala terinfeksi virus diambil dari koleksi tanaman nilam di rumah kaca Balittro. Sampel daun diekstraksi asam nukleat totalnya (RNA+DNA). Asam nukleat total diamplifikasi dengan teknik one step reverse transcriptase polymerase chain reaction (RT-CR) menggunakan primer spesifik gen coat protein (CP) CMV. Produk PCR berukuran 650 pb (pasang basa) dirunut sekuen nukleotidanya serta dianalisis homologi dan hubungan filogenetikanya dengan sekuen isolat-isolat CMV yang ada di GenBank. Sekuen nukleotida dan asam amino gen CP CMV asal nilam menunjukkan persentase kemiripan terbesar (97,1 dan 97,7%) dengan isolat um -Jepang. Analisis filogeni menunjukkan bahwa CMV asal nilam berkerabat sangat dekat dengan CMV isolat um-Jepang dengan nilai bootstrap 100%, dan berada dalam satu kelompok dengan isolat-isolat CMV subgrup IB. Keberadaan CMV subgrup IB pada tanaman nilam perlu diwaspadai karena subgrup I lebih virulen dibandingkan subgrup II. Penelitian ini merupakan laporan pertama karakterisasi molekuler CMV nilam, baik di Indonesia maupun di luar negeri, yang dapat digunakan sebagai salah satu dasar pengambilan tindakan pengendalian.Kata kunci: analisis homologi, pohon filogeni, nukleotida, asam amino

IDENTIFIKASI MOLEKULER CUCUMBER MOSAIC VIRUS (CMV) ASAL TANAMAN NILAM (Pogostemon cablin) / Molecular Identification of Cucumber Mosaic Virus Infecting Patchouli (Pogostemon cablin) Miftakhurohmah Miftakhurohmah; I Dewa Nyoman Nyana; Tri Asmira Damayanti; Rita Noveriza
Jurnal Penelitian Tanaman Industri Vol 23, No 1 (2017): Juni, 2017
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v23n1.2017.11-17

Mosaic disease on Indonesian patchouli associated with infection of TeMV, BBWV2, CymMV and CMV. TeMV, BBWV2 and CymMV has been identified molecularly, while CMV just was detected serologically. The objective of this study was to identify CMV from patchouli by molecular approach based on CP gene nucleotide sequence Leaf samples were collected from three mosaic symptomatic patchouli plants in greenhouse of Balittro. Leaf samples were extracted for the total nucleic acids (RNA + DNA). Nucleic acids were amplified using specific primer for CP gene of CMV by one step RT-PCR technique. The DNA of PCR product with the size of ~ 650 bp was directly sequenced and analyzed for its homology with sequences of CMV isolates extracted from Gene Bank. CMV CP gene from patchouli showed the highest of nucleotide and amino acid sequence similarities, 97,1 and 97,7% respectively, with um Japanese isolates. Phylogenetic tree analysis revealed that CMV from patchouli was closely related with um-Japanese isolate with 100% bootstrap value, and clustered with another CMV isolates in subgroup IB. Since the CMV subgroup I was more virulent than subgroup, it is necessary to increase the awareness of the CMV occurrence in another plant.Key words : homology analysis, phylogeny tree, nucleotides, amino acid AbstrakPenyakit mosaik pada tanaman nilam di Indonesia berasosiasi dengan infeksi Telosma mosaic virus (TeMV), Broad bean wilt virus 2 (BBWV2), Cymbidium mosaic virus (CymMV) dan Cucumber mosaic virus (CMV). TeMV, BBWV2 dan CymMV sudah diidentifikasi secara molekuler, sedangkan CMV baru terdeteksi secara serologi. Karakte risasi molekuler setiap virus diperlukan sebagai salah satu dasar pengambilan tindakan pengendalian. Penelitian ini bertujuan untuk mengidentifikasi CMV asal tanaman nilam secara molekuler berdasarkan sekuen nukleotida gen CP. Tiga sampel daun nilam bergejala terinfeksi virus diambil dari koleksi tanaman nilam di rumah kaca Balittro. Sampel daun diekstraksi asam nukleat totalnya (RNA+DNA). Asam nukleat total diamplifikasi dengan teknik one step reverse transcriptase polymerase chain reaction (RT-CR) menggunakan primer spesifik gen coat protein (CP) CMV. Produk PCR berukuran 650 pb (pasang basa) dirunut sekuen nukleotidanya serta dianalisis homologi dan hubungan filogenetikanya dengan sekuen isolat-isolat CMV yang ada di GenBank. Sekuen nukleotida dan asam amino gen CP CMV asal nilam menunjukkan persentase kemiripan terbesar (97,1 dan 97,7%) dengan isolat um -Jepang. Analisis filogeni menunjukkan bahwa CMV asal nilam berkerabat sangat dekat dengan CMV isolat um-Jepang dengan nilai bootstrap 100%, dan berada dalam satu kelompok dengan isolat-isolat CMV subgrup IB. Keberadaan CMV subgrup IB pada tanaman nilam perlu diwaspadai karena subgrup I lebih virulen dibandingkan subgrup II. Penelitian ini merupakan laporan pertama karakterisasi molekuler CMV nilam, baik di Indonesia maupun di luar negeri, yang dapat digunakan sebagai salah satu dasar pengambilan tindakan pengendalian.Kata kunci: analisis homologi, pohon filogeni, nukleotida, asam amino

Title

Found 30 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 30 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 30 Documents
Search