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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Agrikultura Jurnal Natur Indonesia Cropsaver : Journal of Plant Protection Agrovigor: Jurnal Agroekoteknologi Jurnal Penelitian Tanaman Industri Jurnal Ilmu-Ilmu Pertanian Indonesia Jurnal Agroteknos Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Entomologi Indonesia Jurnal Penelitian Tanaman Industri (Littri)

DJOKO PRIJONO

Departemen Proteksi Tanaman, Fakultas Pertanian Institut Pertanian Bogor, Kampus IPB Dramaga, Bogor 16680

Author-ID : 255063

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Industrial & Manufacturing Engineering Medicine & Pharmacology

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Aktivitas insektisida ekstrak buah Piper aduncum L. (Piperaceae) dan Sapindus rarak DC. (Sapindaceae) serta campurannya terhadap larva Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) Yan Yanuar Syahroni; Djoko Prijono
Jurnal Entomologi Indonesia Vol 10 No 1 (2013): April
Publisher : Perhimpunan Entomologi Indonesia

Crocidolomia pavonana is an important pest of Brassicaceous vegetable crops. Botanical insecticides are potential alternatives for the control of C. pavonana pest. Insecticidal activity of Piper aduncum and Sapindus rarak fruit extracts as well as their mixtures was evaluated against C. pavonana larvae in the laboratory. Ethyl acetate P. aduncum (EtPa) extract as well as methanolic S. rarak (MeSr) and aqueous S. rarak (AqSr) extracts were tested separately and in mixtures against second-instar larvae C. pavonana by a leaf-residue feeding method. At the LC95 level - 72 hours after treatment (HAT), EtPa extract was about 14.5 and 12.8 times, respectively, more toxic to C. pavonana larvae than MeSr and AqSr extracts. At the LC95 level, EtPa + MeSr extract mixture (1 : 10 w/w) was about 1.64 times more toxic to the test larvae than EtPa + AqSr extract mixture (1 : 10 w/w). Based on combination index at 48, 72, and 96 HAT, EtPa + MeSr extract mixture at LC50 level as well as EtPa + AqSr extract mixture at LC50 and LC95 levels had additive joint action, whereas EtPa + MeSr extract mixture at LC95 was weakly synergistic. In addition to lethal effect, the treatment with all test materials delayed the development of C. pavonana larvae from second-instar to fourth instar. Thus, EtPa, MeSr, and AqSr extracts as well as the mixtures between EtPa and S. rarak extracts are potential alternatives for the control of C. pavonana pest.

Status dan Mekanisme Resistensi Biokimia Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) terhadap Insektisida Organofosfat serta Kepekaannya terhadap Insektisida Botani Ekstrak Biji Barringtonia asiatica DANAR DONO; SYAFRI ISMAYANA; IDAR IDAR; DJOKO PRIJONO; IKHA MUSLIKHA
Jurnal Entomologi Indonesia Vol 7 No 1 (2010): April
Publisher : Perhimpunan Entomologi Indonesia

An examination of insect resistance was determined by several steps, i.e. standard sensitivity, resistance diagnosis, and determination of resistance level. Each phase was tested with feeding and residue contact methods at glass tube. Resistance ratio (RR) was determined by comparing LC50 value of field population with standard population. Field population of C. pavonana was classified resistant if it had RR 5 4. Biochemistry analysis of resistance was conducted to population of C. pavonana showing resistance to prophenophos insecticide. The activity analysis of acetylcholine esterase (ACHE), esterase, and Glutation Stransferase was done with spectrophotometer method. Insect which are resistant to prophenophos insecticide was tested for its sensitivity to Barringtonia asiatica seed extract. Result indicated that C. pavonana population from Pengalengan showed resistance to prophenophos synthetic insecticide. Using contact test, the highest resistance ratio value was 4.04, while by feeding assay the RR was 2.78. The study on biochemical resistance mechanisms of each field population of C. pavonana showed various activities of enzymatic detoxification. This could be due to the difference in the kind of insecticides exposed to each field population of C. pavonana. Since RR value from the contact test was higher than that of the feeding test, the resistance development of C. pavonana to synthetic insecticides was probably caused by physiological and biochemical changes in insect cuticle rather than the activity of detoxification enzyme. Methanolic seed extract of B. asiatica can be used as an alternative of resistance management of C. pavonana to prophenophos synthetic insecticide.

Potensi Insektisida Pasak Bumi (Eurycoma longifolia Jack) terhadap Hama Kubis Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) Eka Chandra Lina; Arneti Aarneti; Djoko Prijono; Dadang Dadang
Jurnal Entomologi Indonesia Vol 6 No 1 (2009): April
Publisher : Perhimpunan Entomologi Indonesia

The research was conducted at Laboratory of Insect Physiology and Toxicology of Plant Protection Department of Bogor Agricultural University from May to July 2008. Leaves, twigs, barks, and roots of Eurycoma longifolia (Pasak bumi) were extracted with hexane, ethyl acetate, and methanol solvens. The extraction was used single method and level method. Insecticidal activity of each extract has been examined in laboratory to major cabbage pests Crocidolomia pavonana using leaf residual method. Methanol root extract from single method and level method caused 100 and 75.5% mortality of insect test respectively. Hexane and ethyl acetate extracts of leaves, twigs, and barks have low insecticidal activity. The antifeedant effect worked at insect test and varied depends on concentration level. Antifeedant effect also contributed to mortality of larvae along with toxicity of each extract.

Pembuatan formulasi ektrak kulit batang Calophyllum soulattri dan aktivitas residu terhadap larva Crocidolomia pavonana Edy Syahputra; Djoko Prijono
Jurnal Entomologi Indonesia Vol 5 No 2 (2008): September
Publisher : Perhimpunan Entomologi Indonesia

One option to lessen the problems arising from the use of synthetic insecticides is to exploit plants that have insecticidal activities, such as species of Calophyllum(Clusiaceae). The study has been conducted to prepare insecticidal formulation from C. soulattri bark extract and to evaluate their residual activity against C. pavonana. Formulation prepared by using emulsifier, solvent, and sticking agent. Colour, acidity and emulsion stability were recorded as physic-chemical character of formulation. The result showed that the formulation of C. soulattri bark extract was stable, and their pH was normal. Bioassay of residual activity was done using leaf-residual method. Formulation of bark extract of 66 EC sprayed in suspension concentration of 1% on potted broccoli plant had residual activity around 70.7%-72% with range of half-lives of 9.4-9.6 days.

Aktivitas residu protein Cry1Ac Pada lahan yang ditanami kapas transgenik-Bt di Bajeng dan Soppeng, Sulawesi Selatan Purnama Hidayat; Djoko Prijono
Jurnal Entomologi Indonesia Vol 3 No 1 (2006): April
Publisher : Perhimpunan Entomologi Indonesia

Soil samples were collected from each plot at the two field sites at 0, 1, 3, and 5 months after planting, and at one-month intervals after harvest for three months. Soil suspension from each sample was mixed with agar based diet (1 g soil/20 ml soil-diet mixture) then was presented to neonate larvae of H. armigera. Two reference standards were used, i.e. pure Cry1Ac protein (0.165 x 104 to 1.436 x 105 ng per ml of the proteindiet mixture) and non-Bt-soil spiked with Cry1Ac protein (0.11 x 104 to 9.573 x 104 ng per ml of the soil-diet-mixture). Larval mortality was recorded at 7 days of treatment. In both reference standards, mortality of H. armigera larvae occurred in a concentrationdependent fashion, i.e. larval mortality increased with the increase in Cry1Ac protein concentration, with LC50 of 0.95 x 104 and 1.65 x 104 ng/ml, respectively, for the pure protein and spiked non-Bt-soil standards. Result of the bioassay of soil samples from the field sites showed the residues from both transgenic cotton soils did not cause any mortality (0%) in the test insects. This indicates that the concentrations of Cry1Ac residue in soil at the study sites were very low (in the second standard, Cry1Ac at a concentration of 0.33 x 104 ng/ml caused about 10% larval mortality).

Keberhasilan hidup parasitoid Diadegma semiclausum Hellen dan serangga inangnya Plutella xylostella (L.) terhadap aplikasi ekstrak biji srikaya (Annona squamosa L.) Bonjok Istiaji; Djoko Prijono; Damayanti Buchori
Jurnal Entomologi Indonesia Vol 15 No 1 (2018): Maret
Publisher : Perhimpunan Entomologi Indonesia

Control of Plutella xylostella L. can be done both biologically and chemically. Biological control is generally done by utilizing plant extracts to kill insect pests. This study aims to determine the effect of the extract of seeds on the mortality and development of parasitoid Diadegma semiclausum Hellen and its host, P. xylostella. The effect of Srikaya seed extract on P. xylostella larvae in the initial stage was tested by foliar rest method, while the residue method on the glass surface was used to test the effect of the extract of seed extract of Srikaya on the death of imago D. semiclausum. The effect of seed extract on parasitization and development of D. semiclausum was performed by exposing P. xylostella larvae contaminated by the extract of srikaya seeds in sublethal concentration (LC5 and LC10) in imago D. semiclausum. The results showed that extract of srikaya seeds at a concentration of 0.0632–0.1% at 24-hour contamination affected the death of the larva P. xylostella and the imago parasitoids D. semiclausum. Imago parasitoids are more susceptible to increased concentration of extracts than P. xylostella larvae. The developmental resistance of P. xylostella by Srikaya seed extract at concentrations used is generally unreal. Similarly, the barriers to the development of parasitoid D. semiclausum in hosts contaminated with sublethal concentrations (LC5 and LC10) of seed extract are generally not significantly different. The presence of Srikaya seed extract in the host also has no significant effect on the extent of parasitization, cocoon length, cocoon width, and cocoon weight. Therefore, there is a possibility to chemically and biologically integrate P. xylostella control.

Pengaruh iradiasi sinar gamma terhadap fertilitas Sternochetus frigidus (Fabricius) (Coleoptera: Curculionidae) pada mangga kuini Ade Syahputra; Mustopha Ahad; Abdurakhman Abdurakhman; Joni Hidayat; Djoko Prijono; Indah Arastuti Nasution
Jurnal Entomologi Indonesia Vol 17 No 1 (2020): Maret
Publisher : Perhimpunan Entomologi Indonesia

One of the recognized and safe quarantine treatments for consumers is the use of gamma-ray irradiation [60Co] at a recommended dose requested by the destination country or international agency. The purpose of this study was to test the effectiveness of a generic dose of gamma ray irradiation for sterilizing mango pulp weevil Sternochetus frigidus (Fabricius) on mangoes cv kuini (Mangifera odorata). A total of 300 kg pulp-weevil-infested mango fruits was collected from West Sumatra and Lampung. First experiment was conducted on April to irradiate fruits collected from West Sumatera and second experiment was on May to irradiate fruits collected from Lampung. In each treatment, a box containing 6-8 M. odorata fruits was irradiated with gamma-ray at doses of 50, 100, 150, 200, 300, 400 Gy with three replications. The treatment of gamma-ray irradiation could reduce feeding activity, normal life of insect, egg production and increase mortality of adults. The results of the first and second experiments showed that gamma ray irradiation was able to influence the development of pupae and adults. There were differences in the normal development of adults in the two experiments. The number of normal development of adults on first experiment was higher than on the second experiment. The irradiated pupae at dose of 100 Gy successfully develop to be adults but the adults are unable to produce eggs. Adults irradiated at 150 Gy are still able to lay eggs with the average of 6 eggs (first experiment) and 2,3 larvae (experiment II), 150 Gy average 6 eggs and do not hatch. Doses at 200 Gy no eggs were produced.

Co-Authors . Dadang Abdurakhman Abdurakhman Ade Syahputra Agustin Zarkani Ahmad Sifa Arneti Arneti AUNU RAUF Bambang W. Nugroho Dadang . DADANG DADANG DADANG DADANG DADANG DADANG DAMAYANTI BUCHORI DANAR DONO Danar Dono Dedek Kusvianti EDY SYAHPUTRA Edy Syahputra Edy Syahputra EDY SYAHPUTRA Eka Candra Lina Eka Chandra Lina Erwin Cuk Surahmat Ferdi Ferdi Gracia Mediana GUSTI INDRIATI Gusti Indriati IDAR IDAR IKHA MUSLIKHA Indah Arastuti Nasution Istiaji, Bonjok Joni Hidayat latifah K Darusman Latifah K Darusman Latifah Kosim Darusman Maryani, Sri Riski Maya Anggraini Muhammad Sigit Susanto Mustopha Ahad Nur Alfi Saryanah Partomuan Simanjuntak PURNAMA HIDAYAT R. Dewi Ratna Wulan Safri Ishmayana Shinta Puspitasari Sudarmo Sudarmo Surya, Eliana Diana SYAFRI ISMAYANA Syafrida Manuwoto SYAFRIDA MANUWOTO SYAFRIDA MANUWOTO Wanda Russianzi Widodo Widodo Yan Yanuar Syahroni Yogas Dwi Pratiwi Yuswanti, Lucy

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