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Journal : Cropsaver : Journal of Plant Protection

Resistance Level of Crocidolomia pavonana Against Profenofos Synthetic Insecticide and its Susceptibility To Azadirachta Indica Seed Extract Danar Dono; Yogas Dwi Pratiwi; Safri Ishmayana; Djoko Prijono
CROPSAVER Vol 1, No 2 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.436 KB) | DOI: 10.24198/cs.v1i2.19912

Abstract

This research was conducted to know resistance level of Crocidolomia pavonana  to a profenofos synthetic insecticide and to know susceptibility of C. pavonana  to Azadirachta indica seed extract. The experiment using two bioassay methods, dry film method and feeding method.  Field population of C. pavonana  obtained from 3 villages in Lembang district i.e.Cibogo, Cikole and Kayu Ambon village. The standard insect has been maintenance more than 20 generations in the laboratory as a comparison.  The result of the experiment indicated that population of C. pavonana  form Cikole, Cibogo and Kayu Ambon village were resistant to profenofos insecticide with resistance ratio (RR)  was low (1< RR ≤ 5).  Resistance Ratio of C. pavonana  from the third village were 4.69 and 4.83; 1.02 and 1.1 and 1.16 and 1.9 respectively (dry film and feeding method). The value of the resistance ratio was greater through the feeding assay compared to the contact assay. This indicated that the mechanism of C. pavonana resistance was likely to occur mainly in the digestive tract of insects. Enzime activity of the different sample population of C. pavonana indicated variation in their activity. This was indicated that every sample from different field could develop different mechanism of resistance. The toxicity study of A. indica  seed extract to C. pavonana  indicate that the field population of C. pavonana  from the third village was susceptible to seed extract of A.indica  with value RR of less than one point. Therefore seed extract of A. indica  could be used as alternative control against C. pavonana  which resistant to profenofos synthetic insecticide.Keywords: Resistance mechanism, Resistance ratio, Synthetic insecticide, Botanical insecticide
Synergistic Activity of Mixtures of Piper aduncum Fruit Extract and Three Microorganism-Derived Insecticides against the Diamond Back Moth, Plutella xylostella Wanda Russianzi; Djoko Prijono
CROPSAVER Vol 2, No 1 (2019)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (348.189 KB) | DOI: 10.24198/cs.v2i1.22299

Abstract

Plutella xylostella is an important pest of Brassicaceae vegetable crops. Frequent use of insecticides containing single active ingredients can cause resistance in P. xylostella. This work was done to evaluate the synergism between Piper aduncum fruit extract and three microorganism-derived insecticides, i.e. abamectin, chlorfenapyr, and spinetoram, against P. xylostella from Pacet District, Cianjur Regency. Results of leaf-residue feeding bioassays with 48-h feeding treatment showed that LC95 of abamectin, chlorfenapyr, and spinetoram at 96 h after treatment (HAT) was about 5.8, 7.0, and 1.9-fold higher than their respective field rates. Thus, based on leaf-feeding assays P. xylostella larvae from Pacet-Cianjur were not susceptible to abamectin and chlorfenapyr, but were still fairly susceptible to spinetoram. LC95 of P. aduncum extract at 96 HAT was 0.68% (w/v). Based on combination index at the LC95 level - 96 HAT, mixtures of P. aduncum extract with all three test insecticides were synergistic. Thus, P. aduncum extract is potential to be used as an alternative ingredient to increase the effectiveness of the three test insecticides against P. xylostella.Keywords: Botanical insecticides, cabbage pest, insecticide mixtures, microorganism-derived insecticides, synergism.
Insecticidal activity of the extracts of Piper retrofractum fruit and Tephrosia vogelii leaf and their mixtures against Crocidolomia pavonana Djoko Prijono; R. Dewi Ratna Wulan; Ferdi Ferdi; Nur Alfi Saryanah
CROPSAVER Vol 3, No 2 (2020)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v3i2.31305

Abstract

This laboratory work was carried out to evaluate the insecticidal activity of the extracts of Piper retrofractum (Piperaceae) fruit and Tephrosia vogelii (Fabaceae) leaf and their mixtures against the cabbage head caterpillar, Crocidolomia pavonana. Ground plant materials of the two plant species were extracted separately with n-hexane and methanol. The results of leaf-residue feeding bioassays showed that P. retrofractum (Pr) and T. vogelii (Tv) hexane extracts had strong insecticidal activity against C. pavonana larvae (LC95 < 0.5%) and were more active than their respective methanol extracts. Pr and Tv hexane extract had a moderate and a rather weak contact effect on C. pavonana larvae, respectively. In feeding tests, Pr + Tv (1:1) hexane and methanol extract mixtures indicated synergistic joint effect both at LC50 and LC95 level, whereas in the contact test, the mixture of Pr + Tv (1:1) hexane extract was synergistic at the LC50 level but antagonistic at the LC95 level. In choice tests, antifeedant effects of Pr and Tv hexane extracts at LC25 to LC70 levels on C. pavonana larvae followed a concentration-dependent fashion. Thus, separate or mixed P. retrofractum and T. vogelii extracts are potential alternatives for the control of C. pavonana.