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Journal : JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

AKTIVITAS INSEKTISIDA BAGIAN TUMBUHAN CALOPHYLLUM SOULTTRI BURM.F. (CLU IACEAE) TERHADAP LARVA LEPIDOPTERA Syahputra, Edy; Manuwoto, Syafrida; Darusman, Latifah K; ., Dadang; Prijono, Djoko
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 4, No 1 (2004): Maret, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1953.917 KB) | DOI: 10.23960/j.hptt.1423-31

Abstract

 The objective of this test was evaluate the insecticidal activity of ectract of some parts of Calophyllum soulattri (Clusiaceae) against larvae of three species of Lepidoptera, i. e Crocidolomia pavonana, Plutella xylostela, and Pieris sp.. Extraction of plant materials was done by infusion method using ethanol. The bioassays were conducted by leaf-feeding method. Second-instar larvae were fed extract-treated broccoli leaves of 48 hours, then they were presented with untreated leaves until the surviving larvae larvae reached the fourth-instar stage. The number of dead larvae was recorded daily an larval mortality date were analyzed by probit method. The result showed the gummy bark exudates and bark extract of old and young C. soulattri plants were highly active against C. pavonana. The abrk extact of old C. soulattri plant was also effective against P. xilostella and Pieris sp. The gummy exudates possessed strong insecticidal activity against C. pavonana larvae with LC50 of 0.04% and prolonged the developmental time from second to fourth instar of C. soulattri 2.03-7.25 days compared with control. The bark excudate gave positive respon to alkaloid flavonoid, and tannin test. Futher studies are needed to identify insecticidal compound in those active extracts.
PENGARUH CAMPURAN EKSTRAK AGLAIA HARMSIANA PERKINS DAN DYSOXYLUM ACUTANGULUM MIQ. (MELIACEAE) TERHADAP MORTALITAS DAN OVIPOSISI PLUTELLA XYLOSTELLA (L.) (LEPIDOPTERA: YPONOMEUTIDAE) Yuswanti, Lucy; Prijono, Djoko
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 4, No 1 (2004): Maret, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1645.745 KB) | DOI: 10.23960/j.hptt.141-7

Abstract

Effect of Mixture of Aglaia harmsiana Perkins and  Dysoxylum acuntangum Miq. (Meliaceae) Extracts on Mortality and Oviposition of Plutella xyostella (L.) (Lepidoptera: Yponomeutidae). This study was conducted to evaluate the effect of mixture of extracts of Aglaia harmsiana seeds and Dysoxylum acutangulum leaf petioles on mortality and oviposition of Plutella xyostella (L.) (Lepidoptera: Yponomeutidae). The insecticidal activity of ethyl acetate fraction of A. harmsiana and D. Acutangulum extracts and their mixture (concentration ratio of Ah and Da was 4:1) was evaluated against the thrid-instar P. xylostella was assessed by choice tests using broccoli seddlings. LC50 and LC95 of A. harmsiana extracts against the thirds the+fourth instar larvae were 0.054% and 0.129%, respectively, those of D. acutangulum extract were 0.031% and 0.103%, and those of the the mixture of A. harmsiana and D.acutangulum extract and the mixture at concentrations of 0.06% - 0.38%, 0.3%, and 0.02% - 0.3%, respectively, significantly reduced the number of eggs deposited by the female, P. xylostella on broccoli seedings, with oviposition inhibition indices of 0.30-0.59, 0.28-0.45 and 0.045-0.70. A.harmsiana ectract at all concentrations tested was phytotoxic to broccoli seedlings, whereas D. acutangulum extract was not. Mixture of A. harmsiana and D. acutangulum extract were phytotoxic to broccoli seedlings at concentrations of 0.06-0.3%. Thus, A. hamarsiana extract of mixture containing the extract should be used with caution and their phytotoxicity should be specifically tested on target crops before being used in the field.
INSECTICIDAL JOINT ACTION OF TERNARY EXTRACT MIXTURES OF FOUR SPECIES OF TROPICAL PLANTS AGAINST Plutella xylostella AND Crocidolomia pavonana Prijono, Djoko; Anggraini, Maya; Maryani, Sri Riski; Surya, Eliana Diana
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 1 (2021): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12134-42

Abstract

Insecticidal joint action of ternary extract mixtures of four species of tropical plants against Plutella xylostella andCrocidolomia pavonana. The diamondback moth (DBM), Plutella xylostella, and the cabbage head caterpillar (CHC),Crocidolomia pavonana, were two main pests of cruciferous vegetable crops. Among the viable alternative ingredients thatcould be utilized to control those two pests were eco-friendly botanical insecticides. This study was conducted to evaluatethe joint action of ternary extract mixtures of four tropical plant species, i.e. Piper aduncum (Pa), Piper retrofractum (Pr),Sapindus rarak (Sr), and Tephrosia vogelii (Tv), against DBM and CHC. Results of leaf-residue feeding bioassays showedthat based on co-toxicity ratio at both LC 50 and LC levels, ternary mixtures of Pa extract with Sr and Tv extracts at concentrationratios of 1:5:1, 1:5:2, and 2:5:1 indicated synergistic joint action on DBM larvae. A mixture of Pa, Sr and Tv extracts at a ratio of2:5:1 was also synergistic to CHC at both LC level but antagonistic at the LC 95 50 95 and LC 95 levels. This mixture at the 1:5:1 ratio was synergistic on CHC at the LC level whereas at the 1:5:2 ratio was antagonistic to CHC at both LC levels. Furthermore, ternary mixtures of Pr extract with Sr and Tv extracts at the three concentration ratios were synergistic to CHC. Thus, ternary mixtures of Pa or Pr extract with Sr and Tv extracts at appropriate concentration ratios are potential alternatives for the control of DBM and CHC.
KERENTANAN WERENG BATANG COKELAT (NILAPARVATA LUGENS) DARI ENAM LOKASI DI PULAU JAWA TERHADAP TIGA JENIS INSEKTISIDA Erwin Cuk Surahmat; Dadang .; Djoko Prijono
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 16 No. 1 (2016): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.956 KB) | DOI: 10.23960/j.hptt.11671-81

Abstract

Susceptibility of the rice brown planthopper (Nilaparvata lugens) from six locations in Java to three insecticides. The rice brown planthopper (BPH), Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), is one of the important rice pests in Indonesia. Despite relatively frequent use of insecticides to control this pest, data on BPH resistance to insecticides commonly used by farmers in Indonesia is scarce. This study was conducted to assess the susceptibility of BPH from six locations in Java to three insecticides, i.e. BPMC, imidacloprid, and pymetrozine, and to find out the historical use of insecticides by rice farmers. The first laboratory generations of BPH adults were subjected to insecticide treatments using a leaf dip residual method as described in IRAC Method No 005. Resistance factor (RF) for BPMC and imidacloprid was determined by comparing LC50 of each insecticide against BPH field populations with that against the reference population, whereas the susceptibility of BPH to pymetrozine was assessed based on inhibition of BPH population development. The three insecticides used in this study were commonly used by farmers in the six sampling locations and those insecticides were mostly used twice per rice growing season. BPH populations from all six locations as well as the reference population were susceptible to pymetrozine in which the treatment with pymetrozine at 50 ppm (one-fifth of the recommended field rate) caused 100% inhibition of BPH population development. BPH from five locations showed resistance to imidacloprid (RF > 4), but that from Pasuruan did not (RF 2.0). BPH from Indramayu was resistant to BPMC (RF 6.6) whereas those from the other five locations were not (RF < 4). Exposure of the reference population to a sublethal dose of insecticides (LC50 or IC50) decreased the susceptibility of the third BPH generation to pymetrozine and BPMC but not to imidacloprid.
KEEFEKTIFAN TIGA JENIS INSEKTISIDA NABATI TERHADAP KUTU PUTIH PEPAYA PARACOCCUS MARGINATUS DAN KEAMANANNYA TERHADAP LARVA KUMBANG PREDATOR CURINUS COERULEUS Ahmad Sifa; Djoko Prijono; Aunu Rauf
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 13 No. 2 (2013): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (115.702 KB) | DOI: 10.23960/j.hptt.213124-132

Abstract

Effectiveness of three botanical insecticides against the papaya mealybug Paracoccus marginatus and their safety to the predatory Curinus coeruleus larvae. Preparations of Tephrosia vogelii (Tv) leaf extract (0.5% and 1% w/v), Annona squamosa (As) seed extract (0.5% and 1%), and Cinnamomum multiforum (Cm) leaf essential oil (1% and 2%), and their mixtures (Mix-1: Tv 0.25% + As 0.25% + Cm 0.5%; Mix-2: Tv 0.5% + As 0.5% + Cm 1%) were tested for their effectiveness on third-instar nymphs of Paracoccus marginatus by spraying the test materials on undetached papaya leaves and by direct spraying on the test insects using Potter spray tower. Tv extract was also applied on the test insects placed on undetached papaya leaves. The safety test was done by direct spraying of the test materials on the predatory Curinus coeruleus larvae using Potter spray tower. Tv and As extracts at a concentration of 1% each are potential to be used for the control of P. marginatus. Spraying of T. vogelii extract on the test P. marginatus nymphs placed on papaya leaves was more effective than spraying of the test materials on papaya leaves or direct spraying on the test insects only. The treatment with Cm essential oil required twice the concentration of Tv and As extracts to obtain the same level of effectiveness. The treatment with Mix-2 caused lower P. marginatus mortality than the sum of mortality caused by its components applied separately. Nonetheless, the three botanical insecticides and their mixtures were safe to C. coeruleus larvae. On the other hand, although the synthetic insecticide imidacloprid (neonicotinoid), included in the study for comparison, was effective against P. marginatus, it was also toxic to the predatory C. coeruleus larvae so its use should be avoided or limited.
SEDIAAN INSEKTISIDA CALOPHYLLUM SOULATTRI: AKTIVITAS INSEKTISIDA DAN RESIDU TERHADAP LARVA CROCIDOLOMIA PAVONANA DAN KEAMANAN PADA TANAMAN Edy Syahputra, Djoko Prijono, .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 7 No. 1 (2007): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (122.506 KB) | DOI: 10.23960/j.hptt.17%p

Abstract

Insecticide Preparation of Calophyllum soulattri: Insecticidal and Residual Activity Against Crocidolomia pavonana and Its Savety on Crops. Bioassays were done using leaf-residual method with Crocidolomia pavonana larvae as test insect. The number of dead larvae was recorded daily and was analyzed using probit method. The results showed that methanol extract, and dichloromethane fraction of C. soulattri bark possessed strong lethal effect against C. pavonana larvae with LC50 of 0.15%, and 0.05%, respectively. Dichloromethane fraction of 21 EC and 21 WP sprayed in suspension consentration of 1% on potted broccoli plant had residual activity around 16%-26,7% with range of half-lives of 5.9-7.7 days. Dichloromethane fraction at concentration of 0.2% and 0.3% formulated as EC did not cause phytotoxic to broccoli, tomato, chilli, soybean, red bean, onion, cucumber, and maize. Concerning with this potency, further studies are needed to identify insecticidal compounds.
PENGARUH FRAKSI AKTIF AGLAIA HARMSIANA PERKINS (MELIACEAE) TERHADAP FISIOLOGI LARVA SPODOPTERA LITURA (F.) (LEPIDOPTERA: NOCTUIDAE) Eka Candra Lina, Djoko Prijono, dan Dadang .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 6 No. 1 (2006): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (187.864 KB) | DOI: 10.23960/j.hptt.161-8

Abstract

The effect of active fraction Aglalia harmsiana Perkins (Meliaceae) to physiology of Spodoptera litura (F.) (Lepidoptera : Noctuidae) larvae. This study was conducted to evaluate the physiological interferences in the soybean armyworm Spodoptera litura caused by active fractions of Aglaia harmsiana seed extract. The activity of the test materials was assessed by dietary preparation and topical application method. Repeated fractionations of A. harmsiana extract by chromatographic methods yielded two active fractions, designated as fraction 2-7 (0.0184%) and 2-8 (0.3773%). The results showed that fraction 2-8 had strong insecticidal activity against S. litura larvae, with LC50 by topical application and dietary preparation were 0.49% and 0.0044%, respectively. A sublethal treatment with the active fraction (LC25) reduced the relative growth rate, efficiency of conversion of ingested food, and efficiency of conversion of digested food by 52.9%, 42.9%, 49.6% with topical application and 66.7%, 50%, 63.8% with dietary preparation method. The treatments with that fraction at LC10 and LC25 to the fourth instars reduced the activity of invertase enzyme by 34% and 47%, but increased the activity of trehalase by 6.2% and 12.5% as compared with controls.
KERAGAMAN AKTIVITAS INSEKTISIDA CALOPHYLLUM SOULATTRI BURM. F. (CLUSIACEAE) DARI KALIMANTAN BARAT Edy Syahputra, Djoko Prijono, Fadjar Rianto .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 6 No. 1 (2006): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.472 KB) | DOI: 10.23960/j.hptt.1623-31

Abstract

Diversity of insecticide activity of Calophylum soulattri Burm. F. (Clusiaceae) originate from West Kalimantan. The objective of this research was to evaluate the insecticidal activity of the bark extract of Calophyllum soulattri from West Borneo against Crocidolomia pavonana (F.) (Lepidoptera: Pyralidae) larvae. Extraction of the bark was performed with maceration method using ethanol and continued by acetone reextraction. High performance liquid chromatography (HPLC) with C18 column (reversed-phase) and methanol-water (1:1) as the mobile phase was used to measure the amount of active components. Bioassays were done using leaf-residual method. The results showed that acetone bark extract of 10 sample plants of C. soulattri possessed strong lethal effect against C. pavonana larvae. The lethal effect of the extracts did not differ from each other and the correlation with the content of active components was not significant. The sample plant no. 12 showed the highest yield of acetone extract. Based on both the insecticidal activity and its rendement, it is concluded that the sample plant no. 12 is promising to be used as a parent plant for mass-propagation of source plants
POLYTOMOUS QUANTAL RESPONSE OF CROCIDOLOMIA PAVONANA (F.) (LEPIDOPTERA: PYRALIDAE) TO EXTRACTS OF AGLAIA SPP. AND DYSOXYLUM SPP. (MELIACEAE) Djoko Prijono .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 5 No. 1 (2005): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (134.415 KB) | DOI: 10.23960/j.hptt.151-10

Abstract

Polytomous Quantal Response of Crocidolomia pavonana (F.) (Lepidoptera: Pyralidae) to Extracts of Aglaia spp. and Dysoxylum spp. (Meliaceae). This work was done to study the polytomous binary response of the cabbage head caterpillar Crocidolomia pavonana (F.) to extracts of two species each of Aglaia and Dysoxylum (Meliaceae). Second-instar larvae C. pavonana were fed extract-treated broccoli leaves for 2 days and then the surviving larvae were maintained on untreated leaves until pupation. Relationship between extract concentration and the number of dead larvae in different instars was analyzed using polytomous quantal response analysis based on the conditional logit model. The results showed that twig extract of Dysoxylum acutangulum and seed extract of D. mollissimum possessed strong insect growth regulating activity against C. pavonana larvae as reflected by highly significant responses in the later insect life stages after the feeding treatment was removed, including the significant occurrence of malformed pupae. On the contrary, the feeding treatment with twig extract of Aglaia odorata and seed extract of A. odoratissima resulted in highly significant responses only in the treated larval instar, and after the feeding treatment was removed, responses in the later life stages were insignificant or much less significant. Overall, the above results suggest that Dysoxylum extracts interfered with hormonally-controlled insect development and metamorphosis, whereas the activity of Aglaia extracts was more insecticidal rather than insect growth regulating.
PERKEMBANGAN DAN REPRODUKSI CROCIDOLOMIA PAVONANA (F.) (LEPIDOPTERA: PYRALIDAE) PADA PAKAN ALAMI DAN SEMIBUATAN Novalia Jelita Sari dan Djoko Prijono .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 4 No. 2 (2004): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (128.097 KB) | DOI: 10.23960/j.hptt.2453-61

Abstract

Development and reproduction of Crocidolomia pavonana (F.) (Lepidoptera: Pyralidae) on natural and artificial diets. Development and reproduction of Crocidolomia pavonana on natural and artificial diets were studied in the laboratory (25.2 &plusmn; 0.9 ï‚°C; 84.6% &plusmn; 6.7% RH; ca. 12 h photophase). The natural diets tested were broccoli, cabbage, chinese cabbage, and cauliflower leaves. The artificial diets used were agar&ndash;based general lepidopteran diets mixed with red bean or broccoli leaves, and supplemented with vitamins and microbial inhibitors. Observation of insect development was done since the egg stage. After the eggs hatched, larvae were kept singly in plastic cups and fed with appropriate diets. Fifty larvae were used for each diet. Records were kept with regard to the duration of each larval instar, pupal period, and the pupal weight. The emerging adults were paired, and then the number of dead adults and that of eggs laid were recorded daily. On all the natural diets tested, the larval stage of C. pavonana passed through four instars. The egg incubation, total larval developmental, and pupal period of C. pavonana on chinese cabbage were the shortest compared to those on the other natural diets. The pupal weight, however, was the highest on broccoli, followed by that on chinese cabbage, cabbage, and cauliflower. The female fecundity was also the highest on broccoli (average 258 eggs/female) followed by that on chinese cabbage (212), cauliflower (162), and cabbage (102). Broccoli diet also yielded adults with the longest lifespan although the adult lifespan on broccoli was not significantly different from that on the other natural diets, except that of males on cabbage. C. pavonana failed to develop successfully on six kinds of artificial diets tested. The best artificial diet (broccoli&ndash;based diet with microbial inhibitors 20% of the normal amount) only yielded two males and five females with deformed wings, but none of the emerging females produced eggs.