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All Journal International Journal of Public Health Science (IJPHS) HAYATI Journal of Biosciences Bioscience Jurnal Teknologi Pangan dan Gizi Pharmaciana: Jurnal Kefarmasian Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Indonesian Journal of Biotechnology Journal of Agromedicine and Medical Sciences Biota JURNAL PENGABDIAN KEPADA MASYARAKAT Medical Laboratory Technology Journal Jurnal Biodjati THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST JURNAL GALUNG TROPIKA Jurnal Biologi Tropis BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains JURNAL ILMU KEFARMASIAN INDONESIA Nusantara Science and Technology Proceedings Jurnal Insan Farmasi Indonesia Livestock and Animal Research Journal of Biotechnology and Natural Science Jurnal Katalisator Medical Sains : Jurnal Ilmiah Kefarmasian
Maliza, Rita
Biology Department, Faculty Of Mathematics And Natural Science Andalas University, Padang, 25175
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary

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Journal : THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

 1 
Uji Kualitas DNA Darah Pada Kertas Whatman Yang Diisolasi Dengan CHELEX-100 Serta Variasi Waktu Penyimpanan Rita Maliza; Lutfi Sukma Pratiwi; Dyah Aryani Perwitasari
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i2.7936

Abstract

DNA extraction from dried blood spots was used for molecular analysis. Dried blood spots sample usually used FTA cards, but short-term use would come at a cost. This study aims to identify DNA extraction quality from dried blood spots in Whatman filter paper as an alternative storage sample. This study used 15 samples with three different storage time for 1, 3, and 7 days on 4°C. Dried blood samples were extracted using the Chelex-100 method, and qualitative were identified by electrophoresis. The DNA extraction was used as a template on PCR amplification of the gapdh gene. The result showed that DNA extraction showed band from 1, 3, and 7 days, and PCR amplification showed bands in 200 bp. In conclusions, DNA from dried blood samples was stored for 1, 3 and 7 days in Whatman filter paper were successfully extracted by the Chelex-100 method and can be used as a DNA template for PCR amplification.
Co-Authors Adam Raihan Priambada Adi Wira Septama Adnan, Adnan Afdhal Raihan Aldi Tamara Rahman Alfiatul Laila Alimuddin Tofrizal Allimuddin Tofrizal Angga Kurnia Illahi Anisa Nova Puspitaningrum Aprilia Cassa Nova Athika Darumas Putri Aulia Rahmi Ayu Rosemeilia Dewi Azizah Amatu zikrah Azizah Amatu Zikrah Bayu Tri Murti Bramadi Arya Bramadi Arya Bramadi Arya Chairunnisa Azzahra Azzahra H Diana Fadhilah Diani Fatmawati Dini Cahyani Dyah Aryani Perwitasari Dyah Aryani Perwitasari Efa Refiani Era Refiani Esa Savitri Fadillah Fadillah Fadillah Febriofca Galih Yatalaththov Gina Noor Djalilah Haafizah Dania Haafizah Dania Haafizah Dania Haafizah Dania Haris Setiawan Harry Nugroho Eko Surniyantoro Hevi Horiza Husna Fitri Husna Fitri Husna Fitri Husna Fitri Hylda Ika Wahyuni Ikrimah Nisa Utami Imaniar Noor Faridah Imaniar Noor Faridah Imaniar Noor Faridah Inggita Utami Jaka Pradika Jannati Aulah Kania Agustina Kiky Martha Ariesaka Lalu Muhammad Irham Liana Fairuzannah Mahdiana Listiatie Budi Utami Lutfi Sukma Pratiwi Made Ary Sarasmita Mardiana Puji Lestari Melinda Widianingrum Melinda Widianingrum Melinda Widyaningrum Melodia Rezadhini Moh Mirza Nuryady Muhammad Hamdi Ibrahim Muhammad Idris Muhammad Ilham Hisbullah Muhammad Nazri Janra Nabilah Adzra Fahlevi Nanik Sulistyani Nastiti Rahajeng Ni Made Ary Sarasmita Nofrita Nofrita Nur Aida Nur Rahmah Hidayati Nurul Annisa OKTIRA ROKA AJI Pratama, Kharisma Puti Khairunnajwa Amar Putra Santoso Putri Lestari Rarastoeti Pratiwi Resti Rahayu Reziq Marchellino Irwan Rifai, Fauziah Novita Putri Rihadatul Aisyah Rilwan Efendi Rizma Nurul Akhlas Robby Jannatan Rocky Cheung Rocky Cheung Siti Rokhimah Sumaryati Syukur Sutri Wulansari Safril Syaiful Adam Maulana Tasya Putri Pratama Elisa Tofrizal Tur Rahardjo Wardatul Aini Wirawan Adikusuma Yanti Lusiyanti Yeni Alfiana Ratnasari Yudha Rizky Nuari Yustia Tuti Zozy Aneloi Noli Zozy Aneloi Noni