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Journal : Bioscience

The Effect of Coffee Arabica (Coffea arabica L.) Fruit Skin Extracts on Small Intestine Morphometry of mice (Mus musculus L.) with Ethanol-Induced Rita Maliza; Febriofca Galih Yatalaththov; Haris Setiawan; Listiatie Budi Utami
Bioscience Vol 5, No 1 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202151111571-0-00

Abstract

Consumption of alcoholic beverages can increase ROS and inflammation. In the duodenal, the consumption of alcohol can result in erosions, bleeding, and mucosal injury. Arabica coffee fruit skin contains antioxidant substances, which are expected to have a good role as an antioxidant effect. Currently, unknown effects of alcohol and extract of Arabica coffee fruit skin administration on the damage of the small intestine of mice (Mus musculus L.). This study used an experimental design, and 25 males of mice were divided into five groups of treatment for 15 days. After the treatment, the mice at necropsied and the small intestine were taken for histopathological examination using hematoxyline-eosin staining. The Data analyzed used One Way ANOVA and Duncan's tests. Histopathological observations showed a dose of 250 mg/kg BW experienced the most significant improvement damage of the small intestine of mice compared to other treatments. Histomorphometry measurement, The dose of 250 mg/kg BB showed the high of villi 272,10 ± 13,36 µm, (P<0.05), duodenal villi crypt depth 117,33 ± 30,35 µm (P<0.05) and surface area of small intestinal villi 7,66 ± 0,55 mm2 (P<0.05) was similar with negative control. The conclusion of this study is a dose of 250 mg/kg BW treatment was able to improve the histopathology and morphometry small intestine of mice that were damaged due to consumption of ethanol 15%.
Antibacterial Activity of Coffee Arabica (Coffea arabica L.) Fruit Skin Methanol Extract On Bacteri Eschericia coli and Staphylococcus aureus Rita Maliza; Jannati Aulah; Oktira Roka Aji
Bioscience Vol 4, No 2 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202042108692-0-00

Abstract

Coffee fruit skin is a waste from coffee bean processing. Arabica coffee bean extract (Coffea arabica L.) is known to contain anthocyanin compounds, polyphenols, beta-carotene, and vitamin C which have the potential as antimicrobial agents. The purpose of this study is identification and anaysis the optimal concentration of Arabica coffee peel extract which inhibits the bacteria Escherichia coli and Staphylococcus aureus. This study used an experimental method consisting of 3 extract concentration treatments, positive control, and negative control with 3 repetitions. The treatments tested were 25%, 50%, 75% coffee fruit peel, negative control (sterile distilled water) and positive control (Ampicillin 2 µg / ml). The bacterial test was carried out using the in vitro method. The parameters measured were the amount of inhibition zone (mm) formed on the Mannitol Salt Agar (MSA) and Mac Conkey media. Analysis of research data using the One-way ANOVA test and Duncan test at α = 0.05. The results showed that the optimum concentration of the extract against Staphylococcus aureus was 50%, while the optimum concentration of Escherichia coli was 25%. The results of this study indicate that the extract can be an antibacterial that can inhibit the growth of Escherichia coli and Staphylococcus aureus bacteria optimally.  
Co-Authors Adam Raihan Priambada Adi Wira Septama Adnan, Adnan Afdhal Raihan Aldi Tamara Rahman Alfiatul Laila Alimuddin Tofrizal Allimuddin Tofrizal Angga Kurnia Illahi Anisa Nova Puspitaningrum Aprilia Cassa Nova Athika Darumas Putri Aulia Rahmi Ayu Rosemeilia Dewi Azizah Amatu zikrah Azizah Amatu Zikrah Bayu Tri Murti Bramadi Arya Bramadi Arya Bramadi Arya Chairunnisa Azzahra Azzahra H Diana Fadhilah Diani Fatmawati Dini Cahyani Dyah Aryani Perwitasari Dyah Aryani Perwitasari Efa Refiani Era Refiani Esa Savitri Fadillah Fadillah Fadillah Febriofca Galih Yatalaththov Gina Noor Djalilah Haafizah Dania Haafizah Dania Haafizah Dania Haafizah Dania Haris Setiawan Harry Nugroho Eko Surniyantoro Hevi Horiza Husna Fitri Husna Fitri Husna Fitri Husna Fitri Hylda Ika Wahyuni Ikrimah Nisa Utami Imaniar Noor Faridah Imaniar Noor Faridah Imaniar Noor Faridah Inggita Utami Jaka Pradika Jannati Aulah Kania Agustina Kiky Martha Ariesaka Lalu Muhammad Irham Liana Fairuzannah Mahdiana Listiatie Budi Utami Lutfi Sukma Pratiwi Made Ary Sarasmita Mardiana Puji Lestari Melinda Widianingrum Melinda Widianingrum Melinda Widyaningrum Melodia Rezadhini Moh Mirza Nuryady Muhammad Hamdi Ibrahim Muhammad Idris Muhammad Ilham Hisbullah Muhammad Nazri Janra Nabilah Adzra Fahlevi Nanik Sulistyani Nastiti Rahajeng Ni Made Ary Sarasmita Nofrita Nofrita Nur Aida Nur Rahmah Hidayati Nurul Annisa OKTIRA ROKA AJI Pratama, Kharisma Puti Khairunnajwa Amar Putra Santoso Putri Lestari Rarastoeti Pratiwi Resti Rahayu Reziq Marchellino Irwan Rifai, Fauziah Novita Putri Rihadatul Aisyah Rilwan Efendi Rizma Nurul Akhlas Robby Jannatan Rocky Cheung Rocky Cheung Siti Rokhimah Sumaryati Syukur Sutri Wulansari Safril Syaiful Adam Maulana Tasya Putri Pratama Elisa Tofrizal Tur Rahardjo Wardatul Aini Wirawan Adikusuma Yanti Lusiyanti Yeni Alfiana Ratnasari Yudha Rizky Nuari Yustia Tuti Zozy Aneloi Noli Zozy Aneloi Noni