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All Journal International Journal of Public Health Science (IJPHS) HAYATI Journal of Biosciences Bioscience Jurnal Teknologi Pangan dan Gizi Pharmaciana: Jurnal Kefarmasian Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Indonesian Journal of Biotechnology Journal of Agromedicine and Medical Sciences Biota JURNAL PENGABDIAN KEPADA MASYARAKAT Medical Laboratory Technology Journal Jurnal Biodjati THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST JURNAL GALUNG TROPIKA Jurnal Biologi Tropis BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains JURNAL ILMU KEFARMASIAN INDONESIA Nusantara Science and Technology Proceedings Jurnal Insan Farmasi Indonesia Livestock and Animal Research Journal of Biotechnology and Natural Science Jurnal Katalisator Medical Sains : Jurnal Ilmiah Kefarmasian
Maliza, Rita
Biology Department, Faculty Of Mathematics And Natural Science Andalas University, Padang, 25175
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary

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Journal : Jurnal Katalisator

 1 
UJI KUALITAS DNA METODE CHELEX-SAPONIN DARI SAMPEL FTA CARD Rita Maliza
JURNAL KATALISATOR Vol. 6 No. 1 (2021): jurnal Katalisator Volume 6 No 1, 2021
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (633.423 KB) | DOI: 10.22216/katalisator.v6i1.266

Abstract

ABSTRAK Diabetes Melitus (DM) merupakan penyakit yang ditandai dengan kadar gula darah yang tinggi (hiperglikemia) akibat gangguan kerja insulin, gangguan insulin atau kombinasi keduanya. Studi Genomik DM di Indonesia khususnya di daerah pedalaman masih jarang dilaporkan karena kesulitan pengambilan sampel whole blood pasien DM. Saat ini, kartu FTA adalah alat yang sangat mudah untuk mengumpulkan, mengirim dan menyimpan sampel biologis. Penelitian ini bertujuan untuk mengetahui kualitas DNA yang diisolasi dari kartu FTA menggunakan metode Chelex-Saponin kemudian dilakukan analisis amplifikasi gen target yang berperan dalam DM menggunakan metode PCR. Sepuluh sampel kartu FTA pasien DM tipe 2 digunakan. Amplifikasi PCR menggunakan primer spesifik ABCC8, KCNJ11 dan TCF7L2. Hasil penelitian menunjukkan DNA genom berhasil diekstraksi dari kartu FTA untuk pasien DM tipe 2. Selanjutnya DNA dapat digunakan sebagai template untuk amplifikasi gen ABCC8 (100%), KCNJ11 (60%), dan TCF7L2 (40%). Penelitian ini dapat disimpulkan bahwa metode Chelex-saponin dapat diterapkan sebagai metode ekstraksi DNA sampel darah pada FTA cards pasien DM dan dilanjutkan dengan analisis genomik. Keywords: FTA cards, Chelex-saponin, DM, PCR. ABSTRACT Diabetes Mellitus (DM) is a disease characterized by high blood sugar levels (hyperglycemia) due to insulin work disorders, insulin disorders or a combination of both. Genomic Study on DM in Indonesia, especially in the rural areas, is still rarely reported because of difficulties in whole blood patient DM sample collection. Currently, the FTA card is a very convenient tool for collecting, shipment and storage of biological samples. This study aims to determine the quality of DNA isolated from FTA cards using the Chelex-Saponin method and then amplification analysis of target genes that play a role in DM using the PCR method. Ten samples of FTA cards of DM type 2 patient were used. PCR amplification was used with specific primers ABCC8, KCNJ11 and TCF7L2. Results of this stud showed DNA genome was successfully extracted from FTA cards for patient DM type 2. Furthermore, DNA can be used as a template for amplification of genes ABCC8 (100%), KCNJ11 (60%), and TCF7L2 (40%). This study concludes that the Chelex-saponin method can be applied as a method for DNA extraction of blood samples on FTA cards of DM patients and continued to genomic analysis. Keywords: FTA cards, Chelex-saponin, DM, PCR.
Co-Authors Adam Raihan Priambada Adi Wira Septama Adnan, Adnan Afdhal Raihan Aldi Tamara Rahman Alfiatul Laila Alimuddin Tofrizal Allimuddin Tofrizal Angga Kurnia Illahi Anisa Nova Puspitaningrum Aprilia Cassa Nova Athika Darumas Putri Aulia Rahmi Ayu Rosemeilia Dewi Azizah Amatu zikrah Azizah Amatu Zikrah Bayu Tri Murti Bramadi Arya Bramadi Arya Bramadi Arya Chairunnisa Azzahra Azzahra H Diana Fadhilah Diani Fatmawati Dini Cahyani Dyah Aryani Perwitasari Dyah Aryani Perwitasari Efa Refiani Era Refiani Esa Savitri Fadillah Fadillah Fadillah Febriofca Galih Yatalaththov Gina Noor Djalilah Haafizah Dania Haafizah Dania Haafizah Dania Haafizah Dania Haris Setiawan Harry Nugroho Eko Surniyantoro Hevi Horiza Husna Fitri Husna Fitri Husna Fitri Husna Fitri Hylda Ika Wahyuni Ikrimah Nisa Utami Imaniar Noor Faridah Imaniar Noor Faridah Imaniar Noor Faridah Inggita Utami Jaka Pradika Jannati Aulah Kania Agustina Kiky Martha Ariesaka Lalu Muhammad Irham Liana Fairuzannah Mahdiana Listiatie Budi Utami Lutfi Sukma Pratiwi Made Ary Sarasmita Mardiana Puji Lestari Melinda Widianingrum Melinda Widianingrum Melinda Widyaningrum Melodia Rezadhini Moh Mirza Nuryady Muhammad Hamdi Ibrahim Muhammad Idris Muhammad Ilham Hisbullah Muhammad Nazri Janra Nabilah Adzra Fahlevi Nanik Sulistyani Nastiti Rahajeng Ni Made Ary Sarasmita Nofrita Nofrita Nur Aida Nur Rahmah Hidayati Nurul Annisa OKTIRA ROKA AJI Pratama, Kharisma Puti Khairunnajwa Amar Putra Santoso Putri Lestari Rarastoeti Pratiwi Resti Rahayu Reziq Marchellino Irwan Rifai, Fauziah Novita Putri Rihadatul Aisyah Rilwan Efendi Rizma Nurul Akhlas Robby Jannatan Rocky Cheung Rocky Cheung Siti Rokhimah Sumaryati Syukur Sutri Wulansari Safril Syaiful Adam Maulana Tasya Putri Pratama Elisa Tofrizal Tur Rahardjo Wardatul Aini Wirawan Adikusuma Yanti Lusiyanti Yeni Alfiana Ratnasari Yudha Rizky Nuari Yustia Tuti Zozy Aneloi Noli Zozy Aneloi Noni