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All Journal Jurnal Teknologi Industri Pertanian Jurnal Kimia (Journal of Chemistry) Bionatura Farmaka Pharmaciana: Jurnal Kefarmasian Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Indonesian Journal of Cancer Chemoprevention Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Indonesian Journal of Pharmaceutical Science and Technology Jurnal Ilmu dan Teknologi Peternakan Tropis Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) INDONESIAN JOURNAL OF PHARMACY Jurnal Keselamatan Radiasi dan Lingkungan IDJP (Indonesian Journal of Pharmaceutics) Jurnal Penelitian Pendidikan IPA (JPPIPA) Chimica et Natura Acta Jurnal Biologi Tropis Indonesian Journal of Chemistry Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) Majalah Farmasetika Kumawula: Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Biological Pharmacy Jurnal Sains dan Teknologi Farmasi Indonesia
Muchtaridi Muchtaridi
Departemen Analisis Farmasi Dan Kimia Medisinal - Fakultas Farmasi - Universitas Padjadjaran
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Journal : Pharmaciana: Jurnal Kefarmasian

 1 
Validation of UV-Vis spectrophotometry methode for analysis of apigenin in celery extract (Apium graveolens L.) Yulianto, Asep Nurrahman; Rusdiana, Taofik; Muchtaridi, Muchtaridi; Subarnas, Anas
Pharmaciana Vol 7, No 2 (2017): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.673 KB) | DOI: 10.12928/pharmaciana.v7i2.7124

Abstract

Apigenin is one of the flavonoid compounds contained in celery (Apium graveolens L.) and has been used for the treatment of various diseases. This study aims were to ensure the validity and accuracy of analytical method for apigenin assay in celery extract using UV-Vis spectrophotometry. The flavonoids test of celery extract give a positive result which produces a yellow color that can be drawn by amyl alcohol. The result of TLC test using a mobile phase of chloroform and methanol (9.5 : 0.5) produced Rf value of 0.75. Validation of analytical methods were sensitivity, linearity, limit of detection as well as limit of quantification, accuracy, and precision. Validation of analytical methods showed that sensitivity results as represented by molar extinction (ε) was 77004.35 M-1cm-1. Linearity from calibration curve at concentration of 2, 4, 6, 8, and 10 mgL-1 resulted an equation of y = 0.074x – 0.0011 with coefficient correlation (r) of 0.9996. The limit of detection and limit of quantification were 2.99 mgL-1 and 9.92 mgL-1 respectively with recovery percentage of 97.59% and the coefficient variation values were 1.53%; 2.43% and 1.68%. The average contents of apigenin in the celery extract were of 3.74 ± 0.09%. 
Validation of UV-Vis spectrophotometry methode for analysis of apigenin in celery extract (Apium graveolens L.) Asep Nurrahman Yulianto; Taofik Rusdiana; Muchtaridi Muchtaridi; Anas Subarnas
Pharmaciana Vol 7, No 2 (2017): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.673 KB) | DOI: 10.12928/pharmaciana.v7i2.7124

Abstract

Apigenin is one of the flavonoid compounds contained in celery (Apium graveolens L.) and has been used for the treatment of various diseases. This study aims were to ensure the validity and accuracy of analytical method for apigenin assay in celery extract using UV-Vis spectrophotometry. The flavonoids test of celery extract give a positive result which produces a yellow color that can be drawn by amyl alcohol. The result of TLC test using a mobile phase of chloroform and methanol (9.5 : 0.5) produced Rf value of 0.75. Validation of analytical methods were sensitivity, linearity, limit of detection as well as limit of quantification, accuracy, and precision. Validation of analytical methods showed that sensitivity results as represented by molar extinction (ε) was 77004.35 M-1cm-1. Linearity from calibration curve at concentration of 2, 4, 6, 8, and 10 mgL-1 resulted an equation of y = 0.074x – 0.0011 with coefficient correlation (r) of 0.9996. The limit of detection and limit of quantification were 2.99 mgL-1 and 9.92 mgL-1 respectively with recovery percentage of 97.59% and the coefficient variation values were 1.53%; 2.43% and 1.68%. The average contents of apigenin in the celery extract were of 3.74 ± 0.09%. 
Co-Authors A. A. Putri A. Diantini - A. Khalilah A. L. Triadenda A. Mutalib - Abdul Mutalib Abdul Mutalib Abednego K. Gwiharto Abednego Kristande Abun Abun Abun Abun Abun Ade Rizqi Ridwan Firdaus Ade Zuhrotun Agus Rusdin Agus Rusdin AJENG DIANTINI Aliya Nur Hasanah Ami Tjiraresmi Anas Subarnas Andri Kusmayadi Angga Geganaputra Annisa Atusholihah Annisa Rafashafly Anton Apriyantono Arief Cahyanto Arif Budiman Arif Budiman Asep Nurrahman Yulianto Basuki Hidayat C. Suhandi Carla Florencia Cecep Suhandi Cecep Suhandi Cecep Suhandi Christine Christine CINDY APRILLIANIE WIJAYA Cindy Aprillianie Wijaya Cleopatra D. Semesta Desra Widdy Syafra Devani Olivia Winardi Dewi Permatasari Diah Lia Aulifa Diantini, A. - Dilal Adlin Fadil Dini Rochdiani Dolih Gozali - E. Laelasari E. Novianti Eli Halimah ELI HALIMAH Eli Halimah Ellen N. Yunita Elsa N. Sitinjak Elvan Kamal Ersa Fadhilah F. Ardiansyah Fadil, Dilal Adlin FATHIA NABILA AULANI Fathia Pebriani Ganendra Akbar Hadiyanto Gia A. Faradiba Hafidh Beta Arif Putra Harsoning Tyas Mardisanutol Hasna Chaerunisa Herlina Herlina I Made Jhoni I Made Joni I. R. Hartanti Ida Ayumiati Ida Musfiroh Ida Musfiroh Ida Musfiroh - Imam Adi Wicaksono Ivanna Fauziyah Kusnadi Iyan Sopyan Jessica Tristi Jessyca Sirait Jutti Levita Karyadi Karyadi Karyn Elizabeth Keiichi Motoyama Kevin Reinard Lie L. Pangestu Levita, J. - Lovita Adriani Luthfi Ihsan Sulaeman Luthfi U Setyawati Luthfi U. Setyawati Luthfi Utami Setyawati Luthfi Utami Setyawati Maitsa Alya Fakhirah Martalena Ramli MEGANTARA, SANDRA Meilinda setya praceka MELISSA MELISSA Mentari Luthfika Dewi Mohamad Taufik Ismullah Monica Richelle Herdady Muhammad Ariq Al Faruq Muhammad Fadhil Pratomo Muhammad Raihan Riyaldi Muhammad Syahid Abdillah Muhammad Yusuf Mulyana - - Musfiroh, I. - Mustarichie, R. - Mutakin Mutakin N. Elly Rosilawati N. N. Auliya AS Nabila Alivia Yasmin Nabilah Muhamad Nadia Ulil Fatiya Nadya Dwi Banowati NAELI FARHATY Nasrul Wathoni Nathannael Adrya Hadiwijaya Nazwa N. Mikdar Neli Neli Nia Yuniarsih Norisca A. Putriana Norisca Aliza Putri Norisca Aliza Putri Nurfianti Silvia Nurhanifah Puspitadewi Nursuhud NUZAHA BAQIYATUS SHOLIHAH AZIMAH Petra Pahlawanda Chrisanto Liawardi Randy Rassi Prayoga Ratu Hanifa Fayza Dipadharma Refitha N. Putri Richa Mardianingrum Rina Fajri Nuwarda Risda R Islamiyati Risda Rahmi Islamiaty Riska Febriyanti Ronny Lesmana S. Hidayat Salsa Sagitasa Salsabila Athaya Sandra Megantara SANDRA MEGANTARA Savira Silma Aulia Shafa Nurul Fadilah Siti Nunung Nurulaini Sitti Faza Karima Slamet Budijanto Sri Adi Sumiwi Sri Aguswarini Sri Gustini Husein St. Rohmani Nahariyah Stepanus Massora Sugianto Ayudha Hartono Suhendi, Cece Susyati Susyati Syahla Afaaf Alliyah Syahrul Hidayat T. N. Apriliya Taofik Rusdiana TIANA MILANDA TIARA SALSABILA MAJID Udin. L.Z - Ujang Hidayat Tanuwiria W. Oktavelia Wa Ode Ida Fitriah WARID ALI QOSIM Wiwit Nurhidayah Yasmiwar Susilawati Yulianto, Asep Nurrahman Yuni Nurjanah Zelika Mega Ramadhania