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Populasi Bakteri Dan Jamur Pada Rizosfer Caisim (Brassica juncea L.) Yang Ditanam Di Tanah Dikontaminasi Insektisida Organoklorin Setelah Aplikasi Konsorsia Mikroba Dan Kompos Reginawanti Hindersah; Wawan Rachman; Betty Natalie Fitriatin; Dedy Nursyamsi
Agrologia Vol 3, No 2 (2014): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v3i2.246

Abstract

Organochlorine insecticide was banned for agriculture but nowadays its residue was still found  in some agricultural soil. One of ways to lowering organochlorine residue in soil is by used of degrading-organochlorine microbial consortia accompanied with compost application. However, application of exogenous microbes might affect bacterial and fungal population in soil. The pot experiment has been set up to verify the influence of Pseudomonas mallei and Trichoderma sp. and compost on total bacteria and fungi on rhizosphere of Cai Sim (Brassica juncea L.) grown on Andisols which were contaminated with organochlorine insecticide of heptachlor, endosulfan, dieldrin and DDT. Experimental design was Factorial Randomized Block Design with three replicates. The first factor was microbial consortia of P.mallei and Trichoderma sp., and the second one was kind of compost. The result showed that effect of microbial consortia on total bacteria population was determined by kind of compost. Total bacterial population in rhizosphere of Cai Sim grown with cow manure compost and microbial consortia was more increased.  However, microbial consortia as well as any kind of compost did not influence total fungal population in Cai Sim rhizosphere. In this experiment, total bacterial and fungal population in rhizosphere reached 109 cfu g-1 and  104 cfu g-1 respectively, indicating that major microbial population in rhizosphere was  in the normal range.
Optimasi Produksi Inokulan Cair Trichoderma harzianum Berbasis Molase Reginawanti Hindersah; Wilhemina Rumahlewang; June A Putinella; A Marthin Kalay; Abraham Talahaturuson
Agrologia Vol 4, No 2 (2015): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v4i2.202

Abstract

Trichoderma harzianum as a potential biological agent and are widely used to control diseases in various agricultural crops and plantations. This research goal of getting the best growth of Trichoderma harzianum on molasses-based liquid medium. The treatments tested was added 0.05%, 0.1% and 1% liquid pure culture of Trichoderma harzianum each in 500 ml of sterile liquid medium containing 5% molasses (v/v) and 5% commercial yeast (b / v), and incubated for five days. The results showed that Trichoderma harzianum growth with the highest number of spores occur if the molasses-based liquid medium supplemented with 1% Trichoderma harzianum.
Pertumbuhan Tanaman Kacang Tanah (Arachis hypogea L. Merril) Setelah Aplikasi Azotobacter chroococcum Dan Pupuk NPK Dewikusuma Ikhsani; Reginawanti Hindersah; Diyan Herdiyantoro
Agrologia Vol 7, No 1 (2018): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v7i1.351

Abstract

The combination of biological fertilizers and chemical fertilizers can support the concept of sustainable integrated farming and minimize the adverse impacts of chemical fertilizers. Azotobacter sp. is a soil microbe that used as a biological fertilizer because it can fix N and produce phytohormones which are crucial for plant growth. This research aims to study the growth of peanut plants after Azotobacter chroococcum and NPK fertilizer application. This research was conducted on July-November 2016 in field trials of PT. Pupuk Kujang, Cikampek, West Java with a height of 25 m above sea level. The experiments were performed using a randomized block design with three treatments, 2 L / ha Azotobacter chroococcum, 300 kg / ha of NPK and 2 L / ha Azotobacter chroococcum + 150 kg / ha of NPK with four replications. The results showed treatment with a dose of 2 L / ha Azotobacter chroococcum + 150 kg / ha of NPK fertilizer can increase the growth of plant height and root dry weight, whereas treatment with a dose of 300 kg / ha of NPK most improve plant shoot dry weight. Keywords: Azotobacter chroococcum, NPK, Plant Growth. 
Perubahan Kadar N Tersedia Dan Populasi Azotobacter Di Rizosfer Sorgum (Sorghum bicolor L.) Yang Ditanam Di Dua Ordo Tanah Dengan Inokulasi Azotobacter sp. Reginawanti Hindersah; D. A Sulaksana; D Herdiyantoro
Agrologia Vol 3, No 1 (2014): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v3i1.255

Abstract

Bioagumentation by used of nonsimbiotic N­2 fixing Azotobacter  is a way to enhance soil N availability in sustainable Agricultural land.  A green house experiment has been done to verify effect of Azotobacter sp. inoculation in two soil order, Inceptisols and Entisols, on NH4+danNO3- content , N uptake as well as Azotobacter population in rhizosphere soil of sorghum (Sorghum bicolor L.). Experiment was set up in Randomized Block Design with eight treatments and three replicates, sorghum was maintained in green house until maximum vegetative phase. Resuls showed that Azotobacter sp. AS4 was more enable to increase the availability of soil N rather than isolate AS3.  Bacterial  bioaugmentation with Azotobacter sp. AS4 on Inceptisols increased more NO3-, Azotobacter sp. population in soil rhizosphere and shoot height of sorghum genotype 2.24. 
Pengaruh Bahan Organik dan Azotobacter terhadap pertumbuhan Jagung di Tailing terkontaminasi merkuri dari Pulau Buru Reginawanti Hindersah; A. Marthin Kalay; Rafael Osok
Agrologia Vol 7, No 2 (2018): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v7i2.762

Abstract

Characteristics of mineral-mine tailings deposited in agricultural areas do not meet the requirements for food crop cultivation. The objective of the experiment was to obtain the information on the improvement of plant growth planted in mercury-contaminated tailings after adding organic matter and nitrogen fixing bacteria, Azotobacter. The greenhouse trial was set up in randomized factorial design which tested the Azotobacter inoculation consentrations and organic matter doses. There was no interaction effect between the two treatments on the growth of four-week old corn. Both organic matter and Azotobacter had a positive impact on vegetative growth of corn.
Populasi Bakteri Dan Jamur Serta Pertumbuhan Tanaman Teh (Camellia sinensis L.) Pada Dua Jenis Media Tanam Setelah Inokulasi Azotobacter Reginawanti Hindersah; Bagus Adityo; Pujawati Suryatmana
Agrologia Vol 5, No 1 (2016)
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v5i1.191

Abstract

Substituting a part of soil on tea Camellia Sinensis (L.) O. Kuntze) nurseries by using organic matter could minimize the remove fertile soil from nature. Biofertilizing are a way to improve the biological quality of soil and subsequently tea seedling quality. The aim of the research was to determine the effect of planting media composition and biofertilizer doses on total fungal and bacterial population on planting media; as well as tea seedlings growth. The experiment was conducted using a randomized block design consisting of 9 treatments and 4 replications, each replication consisted of three plants. The treatment consisted of soil without biofertilizer (control), soil + 5% biofertilizer, soil + 10% biofertilizer, cocopeat without biofertilizer, cocopeat + 5% biofertilizer, cocopeat + 10% biofertilizer,  filter cake without biofertilizer, filter cake + 5% biofertilizer, and filter cake + 10% biofertilizer. Biofertilizer used in this experiment were Azotobacter vinelandii and Azotobacter chroococcum that was mixed with growing media before planting. Result of the experiment showed that planting media affect microbial populations differently. The population of total bacteria, total fungi and Azotobacter sp. in the filter cake media with 10% biofertilizer was significantly higher than those of other growing media. However, Azotobacter biofertilizer had no effect on seedling growth of tea (Camellia Sinensis (L.) O. Kuntze) clones Gambung-7
Respons Pertumbuhan Vegetatif Jagung Di Tailing Tambang Timah Terkontaminasi Kadmium Setelah Inokulasi Bakteri Indogenus Reginawanti Hindersah; James Matheus
Agrologia Vol 4, No 1 (2015)
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v4i1.211

Abstract

Contaminant levels of cadmium (Cd) in tin mine tailings often exceeds the upper limit.  Relatively high Cd content might  decrease crop yields and threatened food chain. Bioremediation by using  indigenous microbes to lower soil Cd shoud be done prior to use tailings for cultivation. The objective of this experiment was to obtain information about the capacity of  indigenous bacterias to induce bacterial proliferation  in the rhizosphere, corn growth during the early vegetative stage and change the concentration of Cd in soil and plants. Pot experiment was set up in  a Randomized Block Design consist of several combination of  two bacterial isolates and their doses.  Maize seeds were sown in  tailings from the mine site on the island of Bangka and inoculated with indigenous bacteria. The results showed that the bacterial inoculation increased number of bacteria in the rhizosphere  and plant height, but only isolates BKH1 with a dose of 5 mL/plants that increased plant dry weight. Soil Cd content decreased when inoculated by bacteria although not statistically significant, while the effect of inoculation on plant Cd uptake was inconsistent.
Penggunaan Pupuk Organik Cair Sebagai Media Produksi Inokulan Azotobacter Chroococcum Reginawanti Hindersah; H Yulina; Ane Nurbaity
Agrologia Vol 2, No 2 (2013): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v2i2.264

Abstract

Azotobacter chroococcum based biofertilizers have been widely used in the production of environmentally agriculture. For commercial scale, cheap growth media for Azotobcter inoculant  will affect the quality and price of liquid biofertilizer. Growth medium should support cell proliferation as well as its  nitrogen fixation and phytohormone production.  The objective of this study was to determine whether  liquid organic fertilizer (LOF) containing low N can be used as a medium for scaling up  A. chroococcum inoculant without lowering the survival of bacteria and its activity in N2 fixation and phytohormones production. LOF generally contains a complete nutrient although in a small quantities. Experiment was set up in completely randomized design which tested two types of commercial LOF. The results showed that both of  LOF were only able to support cell growth up to 72 hours and at 96 hours cell viability began to decline compared to the control medium,  free-N Ashby. At 72 hour total N and cytokinin  concentration in LOF was lower than those in Ashby media. This study revealed  that LOF  could be used as A. chroococcum growth media, but without optimization of its composition, cell density and cytokinin content would be lower than those of inoculant  in Ashby Media.
Kepadatan Bakteri Total Dan Azotobacter sp Di Rizosfer Selada (Lactuca sativa L.) Setelah Aplikasi Kasing Dan Pupuk NPK Maya Damayani; Reginawanti Hindersah; Yusuf Rahman
Agrologia Vol 5, No 1 (2016): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v5i1.193

Abstract

Organic matter and inorganic fertilizer application are important to maintain the existence of potential microbes in the rhizosphere and maintain the soil quality. A greenhouse experiment has been carried out to determine the effect of  vermicompost and NPK fertilizer on total bacterial and Azotobacter  population in rhizosphere and yield of lettuce (Lactuca sativa L.) grown in soil order of  Inceptisols. The experimental design was a factorial randomized complete block design with three replicates were tested three doses of vermicompost (7.5, 15 and 22.5 t ha-1) and four doses of inorganic fertilizers; NPK (300; 450; 600 and 750 kg ha-1). The results showed that there was no significant interaction between vermicompost and NPK fertilizer on either total bacterial or Azotobacter population, as well as on fresh weight of lettuce. Both vermicompost and NPK fertilizer did not affect the biological parameters. However, the application of 750 kg/ha NPK fertilizer can increased lettuce weight up to 24% compared to that of control. This study suggested that in the relatively poor soil nutrients, lettuce growth was depend on inorganic fertilizers.
Pertumbuhan Dan Hasil Tanaman Sawi (Brassica juncea L) Setelah Aplikasi Pupuk Hayati Tunggal Dan Dan Konsorsium A Marthin Kalay; Reginawanti Hindersah; Abraham Talahaturuson; Riadh Uluputty; Ferra Langoi
Agrologia Vol 4, No 1 (2015): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v4i1.219

Abstract

The use of biofertilizers is important to prevent the negative impact of the synthetic fertilizers in vegetable production.  This study aimed to determine the effect of single biofertilizer  Azotobacter, and consortial biofertilizer which consist of nitrogen fixer bacteria and pohosphate solubilizing bacteria on the growth and yield of green cabbage (Brassica juncea L). The experimental designed was  randomized block design with five replications. Green cabbage seedling were inoculated with biofertilizer Azotobacter and consortial biofertilizer  on the growth and yield of green cabbage. Plants with consortial biofertilizer showed better growth and yield than that received single biofertilizer. Consortial biofertilizer  can increase plant height and shoot fresh weight up to 15.68 % and 17.05 %, while those with  Azotobacter  were 7.77 % and 9.58 % consecutively, compared with plants control.
Co-Authors A Marthin Kalay A Marthin Kalay A. Marthin Kalay A. Marthin Kalay A. Marthin Kalay Abraham Talahaturuson Abraham Talahaturuson Ade Setiawan Adita Rizky Syntianis Agnia Nabila Agung Karuniawan Agus Jacob Agustinus Marthin Kalay Ai Siti Santriyani Akustu, Masako Alia Halimatusy Aliya Zahrah Adawiah Alyani Shabrina Amiruddin Teapon Ane Nurbaity Ani Yuniarti Anne Nurbaity ANNE NURBAITY Anni Yuniarti Anny Yuniarti Antonio Yusuf Arief Harsono Asep Purnama Hidayat Aten Komarya Bagus Adityo Bari, Ichsan Nurul Benito R. Kurnani Benny Joy Betty N. Fitriatin Betty Natalie Fitriatin Betty Natalie Fitriatin Betty Natalie Fitriatin D Herdiyantoro D. A Sulaksana Dadang Epi Sukarsa dan Arief Harsono dan Nuryani Dedeh H. Arief Dedeh Hudaya Arief Dedi Nursyamsi Dedi Nursyamsi Dedi Nursyamsi Dedi Nursyamsi Dedi Nursyamsi Dedy Nursyamsi Dewikusuma Ikhsani Diana Nafitri Cahayaningrum Diky Indra Wibawa Dirga Sapta Sara Diyah Sri Utami DIYAN HERDIYANTORO Diyan Herdiyantoro Diyan Herdiyantoro Dwi Suci Rahayu ELENA F. L. LILIPALY Erni Suminar Etty Pratiwi Fasa Aditya Fauzul Amri Febby Nur Indriani Febby Nur Indriani Fera Siti Meilani Ferra Langoi Fiky Yulianto Wicaksono Gina Nurhabibah Gita Bina Nugraha H Yulina Haris Maulana Harry Rum Haryadi Henry Kesaulya Herman Rehatta Hidiyah Ayu Ratna Ma’rufah Ibnu Haikal Iis Nur Asyiah Imam Mudakir Imran Makatita Indra Herliana Irene A. Ngabalin Ishak, Lily James Matheus June A Putinella June Putinella Juniart Leklioy Lukman Gunarto M Riadh Uluputty Mahdi Argawan Putra Mahfud Arifin Mardiyani Sidayat Marina Jamlean Martha Fani Cahyandito Marthin Kalay Marthin Kalay Maya Damayani Mayang Agustina Meddy Rachmadi Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mudiyati Rahmatunnisa Mulyadi Mulyadi Nadia Nuraniya Kamaluddin Nadia Nuraniya Kamaluddin Nandha Afrilandha NANJAPPA ASHWATH Neni Rostini Neni Rostini Nenny Nurlaeny NENNY NURLAENY Nia Kurniati Nia Kurniati Nia Kurniati Ninda Meiditia Putri Nini Mila Rahmi Nizar Ulfah Nono Carsono Nurfitriana, Nofalia Osok, Rafael M Oviyanti Mulyani Priyanka Asmiran Priyanka Asmiran Priyanka Asmiran PRIYANKA PRIYANKA Probo Condrosari Pudjawati Suryatmana Pujawati Suryatmana Puji Syara Anggia Putri Sri Judiani Purba Rachelita, Nadia Rachmat Harryanto Rara Rahmantika Risanti Rara Rahmatika Risanti Rhazista Noviardi Rhazista Noviardi Riadh Uluputty Rija Sudirja Rija Sudirja Rina Devnita Rita Harni Rita Harni Robby Risamasu Robi Risamasu Rustam Rustam Salsabilla, Camilla Sandra Amalia Riyadi Saon Banerjee Sarita Sarkar Shabrina Rahma Fauzia Soetijoso Soemitro Solihin, Amir Sondi Kuswaryan Suman Samanta Sunardi Sunardi Tarkus Suganda Toto Sunarto Tri Hanggono Achmad Triani Dewi Triani Dewi Triyani Dewi Triyani Dewi Triyani Dewi Tualar Simarmata Vera Oktavia Subarja Vira Kusuma Dewi Wahyuda Rachman, Wahyuda Wawan Kurniawan Wawan Rachman Wilhelmina Rumahlewang Wilhelmina Rumahlewang Wilhemina Rumahlewang Yansen Lakburlawal Yuliati Mahfud Yusi Firmansyah Yusuf Rahman Yusup Hidayat Zellya Handyman