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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Veteriner The Journal of Experimental Life Sciences (JELS) Journal of Indonesian Tourism and Development Studies Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Biotropika Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Journal of Innovation and Applied Technology Research Journal of Life Science Jurnal Ternak Tropika Journal of Development Research Animal Production : Indonesian Journal of Animal Production JURNAL ILMIAH PETERNAKAN TERPADU Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Budapest International Research in Exact Sciences (BirEx Journal) Jurnal Kedokteran Hewan Chalaza Journal of Animal Husbandry Journal of Livestock and Animal Health (JLAH)
Gatot Ciptadi
Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience Physics Social Sciences Transportation Veterinary Other

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Journal : The Journal of Experimental Life Sciences (JELS)

 1 
Oocyte In Vitro Maturation with Crude Sperm Extract Protein of Bull's Spermatozoa Bilqis Bilqis; Sri Rahayu; Gatot Ciptadi
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (985.453 KB) | DOI: 10.21776/ub.jels.2016.006.01.04

Abstract

Oocyte In vitro maturation (IVM) is one of the important parts for in vitro fertilization (IVF). The success of oocyte maturation is influenced by the composit ion and the quality of IVM medium. Culture medium which used to IVM not only influences the oocyte process to reach metaphase II and proceed the fertilization, but also influences to developmental of an embryo. Crude sperm extract has high-level protein kinase and contains some sperm proteins. Crude sperm extracts expected as natural maturation medium that can increase the success of In Vitro Maturation (IVM). The characterization of crude sperm extracts profile with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Oocyte maturation is observed in the 26th hour from the first culture. The result of crude sperm extract characterization obtained from the protein with the molecular weight is 176.8, 63.2, 58.4, 55.3, 52.4, 49.7, 44.6, 38.02, 36.03, 34.15 and 26.8 kDa. Oocyte maturation with concentration of crude sperm extract 1.5 µg.mL-1 with 71.6% matures oocyte and oocyte maturation with concentration of crude sperm extract 2.5 µg.mL-1 with 75% matures oocyte. Keywords: Crude Sperm Extract, In Vitro Maturation, Oocyte
Estrous Cycle Response in Mice (Mus musculus) with CSE (Crude Sperm Extract) Injected Intraperitoneally Enni Mutiati; Sri Rahayu; Gatot Ciptadi; Moch. Nasich
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (976.426 KB) | DOI: 10.21776/ub.jels.2016.006.01.03

Abstract

Sperm protein has an important role in fertilization process. It becomes antigenic when it is injected to body and can increase TNF-α secretion. TNF-α in blood vesssel can inhibit estradiol synthesis. Estradiol has a significant role in repduction cycle, especially in estrous cycle. The study aims to understand the influence of Crude Sperm Extract (CSE) to mice (Mus musculus) estrous cycle. 16 fertile mices strain Balb-C aged 3-4 months, weighed 20-30 g was divided into 4 groups. P0 is a control group injected by PBS, group P1, P2, and P3 injected by CSE with 1.5 mg.ml-1, 2 mg.ml-1, and 2.5 mg.ml-1. CSE is injected intraperitoneally during mice's  diestrus phase. CSE is injected in day 0, day 12, day 24 and observed daily. The data are descriptively analyzed. The results show that CSE with molecule weight between 26.8-176.8 kDa influences estrous cycle. Keywords: Crude Sperm Extract, estrous cycle, folliculogenesis, Mus musculus
The Abnormality of Spermatozoa Goat after Freezing on -80°C Using Tris Diluent Added Combination Hatching Egg Yolk and Amnion Fluid Liza Choirun Nisa; Sri Rahayu; Gatot Ciptadi
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1051.185 KB) | DOI: 10.21776/ub.jels.2018.008.03.01

Abstract

This study aims to determine the combination of the hatching egg yolk (HEY) and amniotic fluid (AF) in Tris diluent to the type of goat spermatozoa abnormalities after frozen at temperature -80°C. This study used four treatments and 6 replications. The semen is diluted using tris extender and added treatment, there are control group (T0) (Tris egg yolk 20%), T1 (15% HEY + 5% AF), T2 (10% HEY + 10% AF), and T3 (5% HEY + 15% AF). After dilution, the semen was equilibrated for 2 hours, then stored using Mr. Frosty and frozen at temperature -80°C in the ultra-freezer for 24 hours. The type of spermatozoa abnormalities type was evaluated on fresh semen and after thawing. The data were analyzed using ANOVA (P<0.05). The results showed that there is no significant difference among treatments. The primary abnormalities in fresh semen, there were macro-cephalic, amorphous, and the secondary spermatozoa abnormalities such as detached head, mid-piece defect, coiled tail, loosehead, shoe-hook tail, and a broken tail. The secondary abnormalities in post-thawing were found both in the control group and treatment, which are detached head, loosehead, coiled tail, shoe-hook tail, and broken tail. The conclusion of this study was the percentage of secondary abnormalities after thawing was increasing both in the control and treatment groups. Keywords: Amniotic Fluid, Hatching Egg Yolk, Mr. Frosty, Type of Spermatozoa Abnormality.
The Effect of Morinda citrifolia L. Fruit Extract Supplemented in to Diluent Tris-Egg Yolk Toward the Abnormality of Goat's Spermatozoa after Freezing at -80°C Apriani Herni Rophi; Sri Rahayu; Gatot Ciptadi
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1116.681 KB) | DOI: 10.21776/ub.jels.2018.008.03.03

Abstract

The aim of this research is to know the effect of diluent Tris-egg yolk medium added with Morinda citrifolia L. extract towards the sperms abnormality after storage at -80°C for 24 hours. The extracts added in diluent Tris-egg yolk medium were 0% (P0), 10% (P1), 20% (P2), and 30% (P3). The result showed that the percentage of sperms abnormality in P0, P1, P2, and P3 were respectively 8.67%, 6.67%, 5.67%, and 10%. P2 was the lowest percentage, while P3 was the highest percentage of sperms abnormality. In addition, this research found twelve kinds of sperms abnormality divided into categories: major abnormality included the broke tail, absent head, detached head, dag defect, tapered head, proximal droplet, round head and abaxial; and minor abnormality included the coiled tail, shoe hook tail, bent tail, and distal droplet. It has been concluded that the allotment of 20% Morinda citrifolia L. extracts in Tris-egg yolk medium able to maintain the sperm morphology at -80°C storage for 24 hours. Keywords: goat, Morinda citrifolia L., post-thawing, spermatozoa.
Effect of Water Clover (Marsilea crenata) Ethanol Extracts on Follicle and Oocyte Diameter of Goat: In Vitro Study Siska Nanda Widhaningrum; Septiawan Putranto; Sri Rahayu; Gatot Ciptadi
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.06

Abstract

Water clover (Marsilea crenata) is one of the herbal plants that has been using in alternative medicine. It possesses pharmacologically active compounds like flavonoid, which has cellular activities such as antioxidant and estrogenic activity. This study aimed to evaluate the impact of water clover ethanol extract (WCE) at different concentrations on the growth of follicles and oocytes based on follicles and oocytes diameter, respectively, after six days of culture. This experimental study used 24 isolated antral follicles (2.5-3.2 mm), which were randomly divided into four groups including control (without supplemented WCE) and experimental groups that supplemented with different concentrations of WCE (21.6, 43.2, and 86.4 µg ml-1) in culture medium for six days culture. The diameter of follicles was measured on days 0, 3, and 6. Additionally, oocytes diameter was also measured on day 6. The results indicate that the mean diameter of antral follicles and oocyte diameter of WCE 43.2 µg ml-1 was significantly increase compared to the other groups (P≤0.05). According to our results, WCE exerts its effect on the growth of the antral follicle and oocyte based on follicles and oocytes diameter respectively in a dose-dependent manner after six days of the antral follicle cultured.  Keywords: antral follicle, flavonoid, in vitro, oocyte, water clover.
Co-Authors Agung Pramana W.M Agung Pramana W.M Agus Budiarto Agus Budiarto Agus Susanto Ali Harris Angelina N. Tethool Anis Mei Munazaroh Anis Mei Munazaroh Apriani Herni Rophi Ardyah Ramadhina Irsanti Putri Ardyah Ramadhina Irsanti Putri Ardyah Ramadhina Irsanti Putri Aris Subagyo Asma Naili Salsabila Aulanni'am Aulanni'am Aulanni'am, Aulanni'am Aulanni’am A Badriyah B Bambang Susilo Bilqis Bilqis Bima Prakasa Dermawan Sutopo Budi Siswanto Dian Ratnawati Doni Herviyanto Eko Wahyudi Enni Mutiati Eva Ari Wahyuni Fadillah Putra I., Sulistyoningtyas I.D. Retnaningtyas Insafitri, I Karim Khalifa Zamuna KIKI FIBRIANTO Kuswati Kuswati Laili Windah Fauziah Lilik Eka Radiati Liza Choirun Nisa M, Nur Ihsan M.F. Wadjdi Marjono, Marjono Marjuki Marjuki Mirsa Ita Dewi Moch. Nasich Mohammad Nur Ihsan Mudawamah Mudawamah Mudawamah Mudawamah Muhammad Arwani Muhammad Nur Ihsan Muhammad Nur Ihsan Muhammad Rizar Zakaria Nur Ihsan Nurgiartiningsih, V. M. A. Nurul Jadid Mubarokati Omar Sambou R. P. Putra Retnaningtyas, irawati Dinasari Rizka Gitta Almaida Rizki Putra Samudra Rizki Putra Samudra Rosidi Azis Rosidi Azis Roy Ardy Colas Napitupulu S. Chuzaemi S. Susilowati S. Suyadi Septiawan Putranto Septiawan Putranto Setyawan, Hendrix Yulis Siska Nanda Widhaningrum Siska Nanda Widhaningrum Sri Hindrawati Sri Rahayu sri rahayu Sri Rahayu Sri Rahayu Sri Rahayu Sri Rahayu Sri Rahayu Sri Rahayu Sri Wahjuningsih Sunaryo Sunaryo Sunaryo, Moch. Aris Sutiman B. Sumitro Sutiman Bambang Sumitro Suyadi Suyadi Suyadi Suyadi Syafrizal Muhammad Syahrul Kurniawan Teguh Wicaksono Tri Eko Susilorini Trinil Susilawati Trinil Susilawati Veronica Margareta Ani Nurgiartiningsih Woro Busono, Woro Wulandari, Eka Shinta Yudit Oktanella, Yudit Zulvado Satria Putra Yuwono