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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Veteriner The Journal of Experimental Life Sciences (JELS) Journal of Indonesian Tourism and Development Studies Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Biotropika Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Journal of Innovation and Applied Technology Research Journal of Life Science Jurnal Ternak Tropika Journal of Development Research Animal Production : Indonesian Journal of Animal Production JURNAL ILMIAH PETERNAKAN TERPADU Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Budapest International Research in Exact Sciences (BirEx Journal) Jurnal Kedokteran Hewan Chalaza Journal of Animal Husbandry Journal of Livestock and Animal Health (JLAH)
Gatot Ciptadi
Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience Physics Social Sciences Transportation Veterinary Other

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Journal : Research Journal of Life Science

 1 
Post Thawing Sperm Quality and Ca+2 Intensity Characters of Local Goat Sperm After Freezing by Simple Method Using Deep Freezing Gatot Ciptadi; Muhammad Nur Ihsan; Sri Rahayu; Sri Wahjuningsih; Anis Mei Munazaroh; R. P. Putra
Research Journal of Life Science Vol 4, No 3 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (775.085 KB) | DOI: 10.21776/ub.rjls.2017.004.03.3

Abstract

The objective of this research was to determine the effect of the simple modified freezing method, 1°C/minute freezing rate with different diluter ration on a post-thawing quality of local goat sperm namely Peranakan Etawah (PE). This work is aimed to study the quality of post-thawing sperm and to characterize the calcium intensity profile of both fresh and post thawing goat sperm. The method used is the experimental design of a laboratory. Freezing semen was performed in 2 main temperatures of -45°C then -196°C respectively using Mr. Frosty (®) System. Early Sperm characters of Ca+2 intensity was performed by Confocal Laser Scanning Microscope (CLSM) through Fluo-3 staining and Ca++ intensity was analysis descriptively. The result showed that post-thawing qualities are considered as good as standard qualities, at least, more than 40% based on Indonesian National Standard (SNI, 2014). The different level diluents commercial of Andromeda used were influenced highly significant (P<0.01). The best diluents ration is 1:4 (v/v) for final sperms stocked at -196°C. However freezing sperm conserved in -196°C is better than in -45°C. Meanwhile, the sperm characters of two condition showed the important variation of Ca+2 intensity, with the length of region measurement of 39.06±4.595 and 32.696±9.011 µm each.  It was concluded that the calcium intensity pattern was varied more and higher in fresh sperm than in freezing sperms. This simple modified method of a freezing system was considered as a feasible alternative method for goat semen in a reason for both for sperm post-thawing quality and practical purposes.
The Comparison of Chromosome Analysis Result by Manual and Software Cytovision Image Analysis Using Simple G-Banding Gatot Ciptadi; Muhammad Nur Ihsan; Sri Rahayu; Veronica Margareta Ani Nurgiartiningsih; Mudawamah Mudawamah; Ardyah Ramadhina Irsanti Putri
Research Journal of Life Science Vol 4, No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (633.513 KB) | DOI: 10.21776/ub.rjls.2017.004.02.3

Abstract

Chromosome analysis or karyotyping is one among powerful methods to characterize normal or abnormal genetic of animals. On the basis of the important chromosome abnormalities and their negative effect in the near future, chromosomal investigation of breeding bull especially for Artificial Insemination (AI) began in different countries. Chromosomal abnormalities are usually considered to be a plague and are to eliminate. In Indonesia, AI implementation in cattle have been started intensively, especially using imported bulls (bos Taurus) i.e. Limousine. A number of cattle breeds have been reported on of the 50 exotic breeds with the problem of 1/29 translocation, then the chromosome analysis to be important to execute. Method performed by collecting blood samples from first generation of crossing breed Madura Cattle vs. Bos Taurus. Sample was added to medium (Karyo MAX Gibco) then placed in CO2 incubator at 38°C. Colcichine was added after 70 hours and kept for 2–3 hours. Slides were prepared and dried then stained with Giemsa. Slides were examined under high phase-contrast microscope, chromosome analysis using cytovision software and manual analysis straightly captured under microscope then arranged. Result of both method of karyotyping may accepted for analysing method of abnormal/normal chromosome. It showed that the 2 N diploid number of chromosome was normal was 60, there were 58 autosomes and 2 sex chromosomes in all cattle observed. It was observed that all cattle tested in this research were normal categories.  The karyotype analysis of all cattle showed that the chromosomes of one cell and different individual each breed varied in size, shape and position of centromere. It was recommended to performed chromosomal investigation of breeding bulls using advanced sophisticated tools of analysis like cytovision image analysis of fluorescent technique. Manual method was recommended only for analysis of normal or abnormal number of chromosome.
Co-Authors Agung Pramana W.M Agung Pramana W.M Agus Budiarto Agus Budiarto Agus Susanto Ali Harris Angelina N. Tethool Anis Mei Munazaroh Anis Mei Munazaroh Apriani Herni Rophi Ardyah Ramadhina Irsanti Putri Ardyah Ramadhina Irsanti Putri Ardyah Ramadhina Irsanti Putri Aris Subagyo Asma Naili Salsabila Aulanni'am Aulanni'am Aulanni'am, Aulanni'am Aulanni’am A Badriyah B Bambang Susilo Bilqis Bilqis Bima Prakasa Dermawan Sutopo Budi Siswanto Dian Ratnawati Doni Herviyanto Eko Wahyudi Enni Mutiati Eva Ari Wahyuni Fadillah Putra I., Sulistyoningtyas I.D. Retnaningtyas Insafitri, I Karim Khalifa Zamuna KIKI FIBRIANTO Kuswati Kuswati Laili Windah Fauziah Lilik Eka Radiati Liza Choirun Nisa M, Nur Ihsan M.F. Wadjdi Marjono, Marjono Marjuki Marjuki Mirsa Ita Dewi Moch. Nasich Mohammad Nur Ihsan Mudawamah Mudawamah Mudawamah Mudawamah Muhammad Arwani Muhammad Nur Ihsan Muhammad Nur Ihsan Muhammad Rizar Zakaria Nur Ihsan Nurgiartiningsih, V. M. A. Nurul Jadid Mubarokati Omar Sambou R. P. Putra Retnaningtyas, irawati Dinasari Rizka Gitta Almaida Rizki Putra Samudra Rizki Putra Samudra Rosidi Azis Rosidi Azis Roy Ardy Colas Napitupulu S. Chuzaemi S. Susilowati S. Suyadi Septiawan Putranto Septiawan Putranto Setyawan, Hendrix Yulis Siska Nanda Widhaningrum Siska Nanda Widhaningrum Sri Hindrawati Sri Rahayu Sri Rahayu Sri Rahayu Sri Rahayu Sri Rahayu sri rahayu Sri Rahayu Sri Rahayu Sri Wahjuningsih Sunaryo Sunaryo Sunaryo, Moch. Aris Sutiman B. Sumitro Sutiman Bambang Sumitro Suyadi Suyadi Suyadi Suyadi Syafrizal Muhammad Syahrul Kurniawan Teguh Wicaksono Tri Eko Susilorini Trinil Susilawati Trinil Susilawati Veronica Margareta Ani Nurgiartiningsih Woro Busono, Woro Wulandari, Eka Shinta Yudit Oktanella, Yudit Zulvado Satria Putra Yuwono