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All Journal INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES International Journal of Biosciences and Biotechnology METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Biologi Udayana SIMBIOSIS Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Journal of Health Sciences and Medicine
Inna Narayani
Program Studi Biologi FMIPA Universitas Udayana Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology

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THE IMPACT OF LAUNDRY DETERGENT AS A METHOD OF WASHING TOWARDS SPERM DNA EXTRACTION RESULT ON COTTON FABRIC Christian Subagya Gunardi; I Ketut Junitha; Inna Narayani
SIMBIOSIS Vol 9 No 2 (2021)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (235.185 KB) | DOI: 10.24843/JSIMBIOSIS.2021.v10.i01.p01

Abstract

The presence of spermatozoa DNA is reliably counted as a solid evidence when dealing with rape cases. However, police department often finds difficulties to obtain it, mainly because it has been destroyed by the perpetrator. Such act can be done by washing semen stains on the clothing with detergent. The purpose of this research is to analyze the impact of detergent as a washing property towards spermatozoa DNA extract on a cotton fabric. Observed variables in this study are quantity and quality of spermatozoa DNA extract from semen stain on cotton fabric. Samples are divided based on two separate treatments; detergent-washed and unwashed. Furthermore, samples are stored with different storage time. This research applied two methods; DNA extraction using kit (Roche) and DNA quantification using spectrophotometer Nanodrop. The research was held at Biomedic Laboratory, Faculty of Medicine, Udayana University, Denpasar with ethical clearance. Seminal plasma sampel was obtained from a donor with informed consent. This research showed that seminal stain washing on cotton fabric doesn't remove the DNA until two-week-storage time.
STRATEGIES TO IMPROVE THE QUALITY OF BIOPHARMACEUTICALS IN CHINESE HAMSTER OVARY (CHO) MAMMALIAN CELLS: A LITERATURE REVIEW Alayna Lillahida Indri Himawan; Adi Santoso; Endah Puji Septisetyani; Pekik Wiji Prasetyaningrum; Nurul Maulida Rahma; Inna Narayani
SIMBIOSIS Vol 11 No 2 (2023)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2023.v11.i02.p11

Abstract

Chinese hamster ovary (CHO) cells are essential cells frequently used to produce biopharmaceutical molecules, especially glycoproteins. Nevertheless, to obtain an efficient production process and effective therapeutic consistency, the right strategy is needed to improve the quality of biological drug products. It is known that all the critical molecules involved in the immune response are glycoproteins, and many therapeutic proteins, such as vaccines, antibodies, and enzymes, require glycans to have total biologic activity. This review discusses the strategy used in CHO cells to modulate sialylation patterns through overexpression of sialyltransferases, CMP-sialic acid transporter, UDP-GlcNAc 2-epimerase (GNE), ManNAc kinase (MNK), and other related enzymes. In addition to modulating biosynthetic pathways to increase sialic acid content with gene overexpression techniques, this review includes methods for inserting glycosylation sites and manipulating glycans to produce the desired glycoforms. An approach through protein fusion techniques with Fc molecules from human IgG is also discussed to increase the protein half-life. Finally, as a strategy to improve the quality of biopharmaceutical molecules, this review also discusses the importance of cell monoclonalization in developing a cell line that truly originates from a single clone. This is a very critical step to obtain batch to batch consistency during the production process of a biopharmaceutical molecule.
Co-Authors A.A.S. A. Sukmaningsih Adi Santoso Ahmad Hamim Sadewa Alayna Lillahida Indri Himawan Anak Agung Ketut Darmadi Anak Agung Yulia Anggiani Christian Subagya Gunardi Endah Puji Septisetyani Fakhrurrasi Fakhri Galih Anindita Kusumatjahja Haryo Seto Wicaksono I Gusti Ngurah Kade Mahardika I Ketut Junitha I KETUT MUKSIN I Made Sara Wijana I NYOMAN MANTIK ASTAWA I Putu Agus Tirta Cahyana I Wayan Arya Mahardika Iriani Setyawati Iriani Setyawati Ketut Ariawati Lucia Emy Octavia M Luthfi Ardiansyah MADE PHARMAWATI Meitini Proborini Wahyuni Ngurah Intan Wiratmini Ni Komang Frely Astuti Ni Luh Eka Setiasih Ni Luh Putu Agustini Ni Putu Eka Krisnayanti Nurul Maulida Rahma Pekik Wiji Prasetyaningrum Putu Desy Yustinadewi R. Niiruri Rasmaya Niruri Rasmaya Niruri Retno Kawuri Revi Juniar Nutrihidayah Rochmalia Juniarti Putri S. Herawati Wayan Tunas Artama YOSSY CAROLINA UNADI Yustiantara, Putu Sanna