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Nurbailis
Andalas University, Padang
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Karakterisasi Genetik TRICHODERMA SPP Indigenus Rizosfir Pisang yang Berpotensi Pengendalian FUSARIUM OXYSPORUM F. SP. CUBENSE Penyebab Penyakit Layu Fusarium pada Pisang Nurbailis, Nurbailis; Martinius, Martinius
Sainstek Vol 11, No 1 (2008): Vol. XI No. 1, September 2008
Publisher : Lembaga Penelitian Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.116 KB)

Abstract

The objective of the research was to characterize the genetic variation of Trichoderma spp. Study of genetic characters used Random Amplified Polymorphic DNA-Polymerase Chain-reaction (RAPD-PCR) that amplicated with four primers (OPA 2, OPA 17, OPB- 05 and Primer 2).  Analysis result of genetic variation of Trichoderma spp make as dendrogram. The result of the research indicated that : 1) Trichoderma spp. had the great genetic variation, 2)Trichoderma isolate from the same region did not always have the same genetic profile, 3) S6 and T1 isolates were effective to inhibit the growth of Fusarium oxysporum f. Sp. cubense  than the others but  had a different genetic profile. Keywords : Trichoderma spp, Fusarium oxysporum f.sp.cubense, karakterisasi genetik
Pengaruh Kolonisasi Trichoderma spp. pada Akar Bibit Pisang terhadap Perkembangan Penyakit Layu Fusarium (Fusarium oxysporum f. sp. cubense) Nurbailis, Nurbailis; Martinius, Martinius
Jurnal Natur Indonesia Vol 13, No 3 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (57.5 KB) | DOI: 10.31258/jnat.13.3.220-225

Abstract

The purpose of the research was to obtain the superior Trichoderma that had ability to colonize root with the resultbeing effective to supress Fusarium wilt desease and promote banana seedling growth. This experiment consistedof 2 factors and 4 replications. The first factor was Trichoderma spp. namely : A. Trichoderma koningii strain S6sh(TK-S6sh), B Trichoderma viride strain T1sk (TV-T1sk) and Trichoderma harzianum strain S10sh (TH-S10sh). Thesecond factor was the kind of banana namely a.Cavendis, b. Barangan and c. Kepok. The observation werecolonization ability, Fusarium wilt desease development and the banana seedling growth. The result showed thatTV-T1sk was the best spesies to colonize all banana seedling root. The highest colonization in Barangan bananaseedling root reached 80%. Trichoderma colonization in banana seedling root could suppress Fusarium wilt diseasedevelopment and increase banana seedling weight. Higher ability of Trichoderma to colonize banana seedling rootcaused lower disease incidence of Fusarium wilt and greater biomass of banana seedling. Interaction betweenTV-T1Sk and Barangan banana was the best in colonization, so they were effective to suppress Fusarium wiltdesease and increase banana seedling biomass.
KESINTASAN BEBERAPA JAMUR ANTAGONIS PADA BUAH CABAI DAN POTENSINYA DALAM MENEKAN PENYAKIT ANTRAKNOSA YANG DISEBABKAN OLEH COLLETOTRICHUM GLOEOSPORIOIDES ., Nurbailis; ., Martinius; Naipinta, Rizka
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 17, No 2 (2017): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (390.069 KB) | DOI: 10.23960/j.hptt.217162-169

Abstract

Persistence of several antagonistic fungus on chilli and its potential to suppress anthracnose disease caused by Colletotrichum gloeosporioides. Anthracnose disease caused by C. gloeosporioides is one of the important diseases on chilli because its directly gives negative impact on chilli production. The aim of this research was to obtain the superior antagonistic fungi that have ability to persist on chili fruit and potential to control anthracnose disease caused by C. gloeosporioides  The experiment consist of two units: 1. Testing of persistence ability of antagonistic fungi on chilli fruit, 2. Testing the potential of antagonistic fungi to suppress anthracnose disease on chilli fruit. Both of the test used Randomized Block Design (RBD) with 10 treatments and 4 replication, each of replication consist of 4 chilies fruit. Those treatments were Trichoderma-PP1, Trichoderma-PP3, Trichoderma-AG2, Trichoderma-PYK3, Paecilomyces-PP6, Paecilomyces-PP7, Paecilomyces-AG4, Paecilomyces-PYK4, Aspergillus PP2 and without antagonistic fungi (control). The result indicated that all antagonistic fungi isolate could persist on chili fruit. The highest persistence were Trichoderma-PP3 and TrichodermaAG2 (95.83%) and the lowest belonged to Paecilomyces-PP7 (50%). Trichoderma-PP3 and Trichoderma-AG2 were the best isolates for suppressing anthracnose disease caused by C. gloeosporioides.
Profil GCMS Senyawa Kimia Ekstrak Metanol Isolat Cendawan Entomopatogen Beauveria Bassiana Dan Akar Cabai Sebagai Pemacu Pertumbuhan Cabai Saragih, Magdalena; Trizelia, Trizelia; Nurbailis, Nurbailis; Yusniwati, Yusniwati
Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Vol 4, No 2 (2020): Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, Juni
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (447.232 KB) | DOI: 10.31289/agr.v4i2.3840

Abstract

The aim of this study was to identify the chemical compound methanol extract of entomopathogenic fungus Beauveria bassiana from insect walang sangit and the chemical compound roots of red chili plants that were able to stimulate the growth of chilli plants after being applied with entomopathogenic fungus   B. bassiana through seed immersion inoculation using GCMS method. The chemical compound identified as a growth booster in   B. bassiana fungus isolates from the insect walang sangit is an Acetic acid Ethanoic acid Ethylic acid Glacial acetic acid CH3COOH, Hexadecanoic acid, methyl ester (CAS) Methylpalmitate Uniphat A60, n-Hexadecanoic acid Hexadecanoic acid n-Hexadecoic acid Palmitic acid, Dianhydromannitol and Ergosta-5,7,22-trien-3-ol, (3.beta., 22E) - (CAS) Ergosterol (CAS), while the chili root contains Acetic acid Ethanoic acid Ethylic acid Glacials acetic acid CH3COOH, Hexadecanoic acid, methyl ester (CAS) Methyl palmitate Uniphat A60, n -Hexadecanoic acid Hexadecanoic acid Palmitic acid, 8, 11- octadecadienoic acid, methyl ester (CAS) METHYL 8, 11-OCTADECADIENOATE, (23S)-ethylcholest-5-en-3.beta.-ol.s It can be concluded that some of the chemical compounds in methanol extracts identified in chili roots have similarities with chemical compounds that exist in   B. bassiana entomopathogenic fungus which are potential as stimulers of chili plant growth
PENERAPAN PENGGUNAAN Trichoderma sp UNTUK PENGENDALIAN PENYAKIT PADI DI NAGARI SIMABUR KECAMATAN PARIANGAN KABUPATEN TANAH DATAR Yanti, Yulmira; Hamid, Hasmiandy; Nurbailis, Nurbailis; Hermeria, Noveriza; Tanjung, Megha Putri
Jurnal Hilirisasi IPTEKS Vol 4 No 4 (2021)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jhi.v4i4.579

Abstract

Nagari Simabur is one of the nagari which is included in the Pariangan District, Tanah Datar Regency with the main sector only in the agricultural sector with the main crop being lowland rice. The problem that is often faced by Simabur farmers is the problem of pests and diseases which are the cause of the low growth and yield of the lowland rice plant. One of the main components of the IPM program that can be used as a biological control agent is the use of Trichoderma indigenous. In addition to the use of biological agents, the system or method of planting also affects the production results of the cultivated plants. The purpose of this community service is to increase public knowledge about the use of Trichoderma which can increase the growth and production of rice plants with the jajar legowo system. The method used in this activity is structured training and direct demonstration in the field. The results obtained from this activity were the production of grain weight/plant clump treated with Trichoderma was higher than the control with a difference of 42.45 grams. The conclusion of this activity is that the growth and yield of plants that were applied with Trichoderma were higher than those that were not treated with Trichoderma (control). The use of rhizobacteria is highly recommended because in addition to being easy to obtain, it also provides a positive value for plant growth and is good for environmental technology around cultivated plants.
Penapisan Cendawan Antagonis Indigenos Rizosfer Jahe dan Uji Daya Hambatnya terhadap Fusarium oxysporum f. sp. zingiberi Nurbailis Nurbailis; Winarto Winarto; Afriani Panko
Jurnal Fitopatologi Indonesia Vol 11 No 1 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.94 KB) | DOI: 10.14692/jfi.11.1.9

Abstract

Ginger rot disease caused by Fusarium oxysporum f. sp. zingiberi is difficult to control because the pathogen is soil borne and is able to form clamidospore as resting structure. The aim of this study was to obtain indigenous antagonistic fungi from ginger rhizosphere which is potential for suppressing the growth of F. oxysporum f. sp. zingiberi. Fungi isolated from ginger rhizosphere were subjected for antagonism assay using dual culture method. Fungi isolates showed capability to inhibit F. oxysporum f. sp. zingiberii were then identified based on morphology characters. Eleven isolates were successfully isolated, but only 9 isolates showed the potentials of suppressing the growth of F. oxysporum f. sp.  zingiberi. All 9 isolates i.e. AB4, GC1, BB1, AB1, AB2, K12, GC3, K11 and GC2 had antibiosis activity, and 3 isolates among them i.e. AB2, BB1 and K11 showed competition mechanism. Based on morphology characters the isolates were identified as Penicillium spp. (4 isolates), Trichoderma spp. (3 isolates), and Aspergillus spp. (2 isolates).
Identifikasi Isolat Rizobakteri Indigenos Potensial Sebagai Agens Biokontrol Ganoderma boninense Yulmira Yanti; Nurbailis Nurbailis; Imam Rifai
Jurnal Agro Industri Perkebunan Volume 9 Nomor 1 Tahun 2021
Publisher : Politeknik Negeri Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jaip.v9i1.1494

Abstract

Rhizobacteria is a group of bacteria that colonize roots, affect growth and control plant pathogens. Based on the results of previous studies, 6 isolates have the best ability to control Ganoderma boninense in oil palm seedlings. It is important to characterize them molecularly. Molecular identifications of the selected rhizobacteria isolates were done using the 16S rRNA gene. The results showed that all isolates were identified to have similarity as 5 different species i.e Bacillus paramycoides, Microbacterium paraoxydans, B. albus, B. cereus, and Serratia marcescens based on NCBI database.
VIRULENSI BERBAGAI ISOLAT JAMUR ENTOMOPATOGEN METARHIZIUM SPP. TERHADAP HAMA PENGGEREK BUAH KAKAO CONOPOMORPHA CRAMERELLA SNELL. (LEPIDOPTERA: GRACILLARIIDAE) . Trizelia; . Nurbailis; Dina Ernawati
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 13 No. 2 (2013): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (375.742 KB) | DOI: 10.23960/j.hptt.213151-158

Abstract

Virulence of various Metarhizium spp. (Deuteromycotina: Hyphomycetes) isolates on cocoa pod borrer pest, Conopomorpha cramerella Snell. (Lepidoptera: Gracillariidae). In Indonesia, Conopomorpha cramerella Snell. (Lepidoptera : Gracillariidae) is a serious pest of cocoa. Entomopathogens such as Metarhizium spp. (Deuteromycotina: Hyphomycetes) can be used to control this pest as an alternative to chemical insecticides. The purpose of this research was to study the virulence of isolates of Metarhizium spp. to pupae of C. cramerella. Nine isolates of Metarhizium spp each with 107 conidia/ml rate were used in the experiment. Pupal mortality was recorded daily. The results showed that isolate MetApCi from chili rhizosphere had the highest virulence causing 96.67% mortality of C. cramerella pupae and with an LT50 of 3.04 days.
Pemanfaatan Bahan Organik sebagai Pembawa untuk Peningkatan Kepadatan Populasi Trichoderma viride pada Rizosfir pisang dan Pengaruhnya terhadap Penyakit Layu Fusarium . Nurbailis; . Martinius
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 11 No. 2 (2011): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (66.997 KB) | DOI: 10.23960/j.hptt.211177-184

Abstract

The aims of the research were to determine the best organic matter as carrier for growth and increasing the density of Trichoderma viride strain TV-T1sk (TV-T1sk) and its influence on Fusarium wilt disease development on banana seedling. This research consisted of two experiments, i.e. (1). Growth testing of TV-T1sk in various organic matter, (2). Various organic matter testing for increasing the density of TV-T1sk in banana rhizosphere and its influence on Fusarium wilt desease development. The treatments were various organic matter, namely rice straw, sugar cane pulp, rice bran+banana plant waste, hull of rice+sugar cane pulp and rice. The variables observed were density and viability of conidia on various organic matter. Propagule density after introduction to banana rhizosphere was determined by observing TV-T1sk propagul from the rizosphere of banana seedling. The influence of density on Fusarium wilt disease development was determined by incubation period, the percentage of symptomized leaves and the intensity of corm damage. The results showed that both sugar cane pulp and rice were the best organic matter for increasing the growth and the density of TV-T1sk after introduction to banana rhizosphere. The density of TV-T1sk propagule on banana rhizophere influenced the development of Fusarium wilt disease.
Keanekaragaman Jamur pada Rizosfer Tanaman Cabai Sistem Konvensional dan Organik dan Potensinya sebagai Agen Pengendali Hayati Colletotrichum gloeosporioides Nurbailis .; Martinius .; Verry Azniza
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 14 No. 1 (2014): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (67.828 KB) | DOI: 10.23960/j.hptt.11416-24

Abstract

ABSTRACTFungal diversity of chili rhizosphere at conventional and organic cropping system and its role as biocontrol agent of Colletotrichum gloeosporioides. The aim of this research was to study fungal diversity in chili rhizosphere at conventional and organic system; to ditermine and identify the fungi that had antagonistic activity against C. gloeosporioides. Diversities of rhizosphere fungi in chili of conventional and organic system were determined by counting the amount of propagule and isolates. Antagonistic activity was examined by dual cultures and slide culture. Identification was determind up to genus level. Propagule density and the amount of isolates in chili rhizosphere of organic system were higher than those in conventional system. Fifty two fungal isolates were found from chili rhizosphere at conventional and organic system, 28 isolates from organic system and 24 isolates from conventional. Ten fungal isolates from chili rhizosphere in organic system and 4 fungal isolates from that in conventional system had antagonistic activity against C. gloeosporioides which cause antracnose on chili. The antagonistic isolates were: Trichoderma, Paecilomyces, Aspergillus and unidentified isolate (X isolate).
Co-Authors . Martinius ., Yusniwati Afriani Panko Annisya Diadinni Cherly Yugu Tanca Darnetty Darnetty Dina Ernawati Eri Sulyanti Haliatur Rahma, Haliatur Hasmiandy Hamid Hermeria, Noveriza Imam Rifai Magdalena Saragih Magdalena Saragih Saragih Martinius . Martinius ., Martinius Martinius Martinius Martinius Martinius Megha Putri Tanjung Muzilatul Nilisma MY SYAHRAWATI Naipinta, Rizka Noveriza Hermeria NOVRI NELLY Reflin Reflin Rummah Yulisyah Rusdi Rusli Saragih Magdalena Saragih, Magdalena Tanjung, Megha Putri Trimuti Habazar Trizelia . Verry Azniza Winarto Winarto Winarto Winarto Yaherwandi Yenny Liswarni Yulmira Yanti Yunisman Yunisman Yunisman Yunisman Yusniwati Yusniwati