Article Per Year (5 Year)
p-Index From 2019 - 2024
2.495
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya The Journal of Experimental Life Sciences (JELS) Biotropika Research Journal of Life Science Natural B Edu Geography Jurnal Pengembangan Teknologi Informasi dan Ilmu Komputer Indonesian Journal of Cardiology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Care : Jurnal Ilmiah Ilmu Kesehatan Jurnal Jamu Indonesia Nusantara Science and Technology Proceedings Berkala Penelitian Hayati
Sri Widyarti
Biology Department, Faculty Of Science And Mathematics, Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Other

Published : 55 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 55 Documents
Search

 2   3   4   5   6 
Analysis Activity of Elephantopus scaber Leaves Extract Against Quantitative Changes of Lymphocytes Cells in BALB/c Mice After Induction of DMBA and Estrogen Lailiyavina Rochmatika; Sri Widyarti; Muhammad Sasmito Djati
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1127.489 KB) | DOI: 10.21776/ub.jels.2019.009.03.08

Abstract

The carcinogenic compounds such as DMBA known for having the ability to promote the DNA damage that affects the growth regulator genes. Therefore, uncontrolled cell growth will occur and change the immune system. Elephantopus scaber extract is considered as an alternative way of handling breast cancer since it capable to affect the work of the immune system. Hence, this study aims to determine the effect of E. scaber extract on quantitative changes of CD4+, CD8+ T cells and proinflammatory cytokines CD4+IFNγ+ and CD4+TNF-α+ after induction of DMBA and estrogen. In vivo experiments were carried out by using 5-6 weeks old of female mice BALB/c strain. The extract of E. scaber leaf powder was obtained from Material Medica Batu, Malang. The experimental animals were divided into five groups such as negative control group (K-), positive control group (K+), mice treated with E. scaber extract for a week (group 1), mice treated with E. scaber extract for 2 weeks (group 2), and mice treated with E. scaber extract for 3 weeks (group 3). Induction of DMBA and estrogen was conducted by injection of DMBA (7.12 dimetylbenz (α) anthracene) 0.56 mg.kgBW-1 and estradiol hormone 0.0504 mg.kgBW-1. Mice were treated with E. scaber extract with a dose of 50 mg.kgBW-1. Lymphocytes were isolated from spleen and observed the CD4+, CD8+ T cells and proinflammatory cytokines CD4+IFN-γ+ and CD4+TNFα+ T cells through flow cytometry analysis. One-way ANOVA (p <0.05) and SPSS were used to analyze this data. The results showed that the induction of DMBA and estradiol hormone affected mice fur loss. It also decreased the immune system by lowering the number of CD4 and CD8 T cells. However, E. scaber extracts increased the number of pro-inflammatory cytokines TNF-α. Furthermore, the DMBA and estradiol induction also promoted the number of CD4+IFN-γ+ in the first week, but decreased the number of CD4+IFN-γ+ at weeks 2 and 3, then increase the number of CD4+IFN-γ+. So, the treatment of E. scaber extracts demonstrated the ability to restore the homeostasis of the immune system in the first and second weeks.  Keywords: CD4+, CD8+, DMBA, estrogen, E.scaber, IFN-γ+, TNF-α+
The Study of Combination Ethanol Extract of Averrhoa bilimbi L. and Momordica charantia L. on CD4+CD25+TGF-β+ Spleenocytes of Hyperglycemia Mice Harits Amrulloh; Bella Novinda; Intan Sartika; Sri Widyarti; Muhaimin Rifa'i
The Journal of Experimental Life Science Vol. 10 No. 3 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.03.05

Abstract

Diabetes mellitus is one of the four priority non-infectious diseases in the world. Plant-based medicine is an alternative treatment with few side effects. Star fruit (Averrhoa bilimbi L.) and bitter melon (Momordica charantia L.) are plants that have anti-hyperglycemic activity. Hyperglycemia produces Reactive Oxygen Species (ROS) that make the β-cells of the pancreas necrosis that decreasing insulin synthesis. The anti-inflammatory activity appears based on the relative levels of CD4+ and CD25+, which are TGF-β-producing regulatory T cells where TGF-β is a mediator that acts as an immunosuppressant. TGF-β would induce CD4 + T lymphocytes into T reg. The purpose of this study is to determine the profile of TGF-β on CD4+ and CD25+ spleenocytes on hyperglycemia mice after ABMC (Averrhoa bilimbi Momordica charantia mix) treatment. Mice were divided into 5 groups, non-diabetic (N), hyperglycemia (H), hyperglycemia with extract doses of 10 mg.kg-1 BW (P1), 40 mg.kg-1 BW (P2), and 160 mg.kg-1 BW (P3). Diabetic mice were obtained after a single injection dose of 145 mg.kg-1 BW streptozotocin (STZ). The result showed that ABMC can reduce blood sugar levels faster and able reduce the number of CD4+TGF-β+ cells in hyperglycemia mice. Keywords: Averrhoa bilimbi L., CD4+TGF-β+, CD25+TGF-β+, hyperglycemia, Momordica charantia L.
Penerapan Metode K-Means untuk Evaluasi Senyawa Anti Kanker Berdasarkan Kajian Proteomik Sel MCF7 Mochammad Pratama Viadi; Marji Marji; Sri Widyarti
Jurnal Pengembangan Teknologi Informasi dan Ilmu Komputer Vol 3 No 5 (2019): Mei 2019
Publisher : Fakultas Ilmu Komputer (FILKOM), Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (447.078 KB)

Abstract

Cancer is a disease that originates from several body cells that can divide without stopping and can spread to surrounding tissues. Then split cell will attack and damage the surrounding tissue. Especially in breast cancer or occupying the top level in cancer. This cancer originates from a glandular tract in the breast which declared a malignant tumor. In the detection this disease can use bioinformatics techniques, namely proteomic studies. Output produced is a grouping of the results of the anti-cancer process on the development of cancer in the form of clustering using the K-Means process which is divided into 2, active and inactive. The results of system testing were carried out using Silhoutte Coefficient and Precision Recall Accuracy. In the Silhoutte Coefficient test getting the optimal cluster is 5 with a value of 0.6827. Whereas in testing the Precision Recall Accuracy, the Precision value was 67.59%, the Recall value was 79.57%, and the accuracy rate was at 57.71%. Using 10-fold cross validation get an average accuracy of 62.26%.
Adaptive Immune Response Stimulation on Nephrolithiasis Mice Model after Treatment of Tempuyung (Sonchus arvensis L.) Leaf Extract Khoirin Maghfiroh; Muhaimin Rifa'i; Sri Widyarti
The Journal of Experimental Life Science Vol. 4 No. 2 (2014)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (890.837 KB) | DOI: 10.21776/ub.jels.2014.004.02.02

Abstract

Calcium crystal accumulation on kidney can cause kidney stone (nephrolithiasis). The oxalate calcium crystal which is deposite on the kidney can trigger inflammation on the epithelial that is able to induce cells death (necrosis). The necrosis is able to cause inflammation and it will affect the body's immune system. Infection agent that comes to the body will be responded by the innate immunity which will be responded later by adaptive immunity. One of herbal agent that is expected to be used to stimulate adaptive immunity response is Tempuyung (Sonchus arvensis) leaf extract. The aim of this study is to find out the change of relative amount of CD4+ T cells, CD8+ T cells, and B (B220) cells on nephrolithiasis mice model after S.arvensis leaf extract. The mice are divided into six groups; control group, placebo, nephrolithiasis, S.arvensis leaf extract for 7 days, nephrolithiasis then it is continued with S.arvensis leaf extract for 7 days, and simultaneous (nephrolithiasis and S.arvensis leaf extract for 3 months). The amount of relative T lymphocyte cells is measured by using BD FACSCalibur FlowcytometerTM. The data is analyzed by using ANOVA one way (p<0.05) using SPSS 16.0 software for Windows. The result shows that there are changes of relative CD4+ T cells, CD8+ T cells, and B (B220) cells on nephrolithiasis mice model after the giving of S.arvensis leaf extract. The treatment of S.arvensis leaf extract on the nephrolithiasis mice model can stimulate the homeostatic activity by suppressing the B cells. Compound of S.arvensis leaf extract that can inhibit of Th1 cells and the increase of Th2 cells by proliferation cells activity. The treatment of S.arvensis leaf extract for 7 days can suppress CD4+. The S.arvensis leaf extract can stimulate adaptive immune response which is caused by immunomodulatory active component.Keywords: Nephrolithiasis, Sonchus arvensis L., leaf extract, adaptive immune response.
The Immunomodulatory Effect of Elephantopus scaber and Sauropus androgynus Extract to Cellular Immune Response in Pregnant Mus muscullus Infected by Salmonella typhimurium Nur Jannah; Moch. Sasmito Djati; Sri Widyarti
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (944.87 KB) | DOI: 10.21776/ub.jels.2016.006.01.02

Abstract

Pregnancy has a high risk and is more susceptible to infectious diseases. E. scaber and S. androgynus contains saponins and flavonoids as an immunomodulatory that can increase the body's immunity. The purpose of this study is to determine the immunomodulatory effects of E. scaber and S. androgynus leaf extract against cellular immune response in pregnant mice infected S. typhimurium. This research used seven treatment groups including negative control (K-) mice without injected S. typhimurium, positive control (K+) mice were injected S. typhimurium and extract treatment with 5 variant doses and dissected on the 12th and 18th days. Lymphocytes was isolated from the blood, then relative number of CD4+ and CD8+ were analyzed with flow cytometry and data continued with ANOVA. Statistical analysis showed that different extract dose and duration of treatment had a significant effect on the number of CD4+ and CD8+ T cells. E. scaber and S. androgynus act as immunomudulatory effect and treatment with combination of extracts E. scaber and S. androgynus 50 mg.kg-1 BW + 112.5 mg.kg-1 BW respectively and treatment with extract of S. androgynus 150 mg.kg-1 BW are the optimum treatment which can restore immune system conditions such as normal pregnancy without infection.Keywords: Cellular Immune, E. scaber, Immunomodulatory, S. androgynus, S. typhimurium.
Co-Authors A'liyatur Rosidah A.A. Ketut Agung Cahyawan W A’liyatur Rosyidah Ahmad Soni Ahmad Soni Amandia Dewi Permana Shita Apik Budi Santoso Arief Azhary Aris Soewondo Aris Suwondo Askandar Tjokroprawiro Attabik Mukhammad Amrillah Attabik Mukhammad Amrillah Bella Novinda Cholilia Abadiatul Masruroh Cholilia Abadiatul Masruroh Daniar Kusumawati Dayu Nirwana Putri Dewi Parlina Ningrum Dian Siswanto Djanggan Sargowo Eko Puji Astuti Erly Nur Aisyah Hafiedz Maulana Harits Amrulloh Intan Sartika Is Karima Karima, Is Khoirin Maghfiroh Khoirin Maghfiroh Lailiyavina Rochmatika Linda Kartika Dewi Lulut Dwi Nurmamulyosari M. Irsyad Nur Maftuch Maftuch Marji Marji Moch Sasmito Djati Mochammad Pratama Viadi Mohammad Mabrur Muhaimin Rifa&#039;i Muhaimin Rifa&#039;i Muhaimin Rifa&#039;i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifai&#039;i Muhamad Aris Widodo Naqiyah A. Mulachelah Noer Hasanah Nunung Harijati Nur Jannah Nur Permatasari Nur Permatasari Nuzulul Hikmah Radita Intan Aisyah Pratiwi Rasjad Indra Retno Susilowati Retty Ratnawati Ria Rismawati Rifa'i, Muhaimin Rike Wahyuningroom Rike Wahyuningroom Ririn Rochmawati Rizki Amalia Rodiyati Azrianingsih Rodiyati Azrianingsih Rosidah, A'liyatur Rosyidah, A’liyatur Satuman Satuman Serafinah Indriyani Sherry Aristyani Siti Imama Khoiriyah Sofy Permana Soni, Ahmad Sri Rahayu SRI RAHAYU Sri Rahayu Sri Rahayu Sri Rahayu Sri Rahayu Suroso Suroso Sutiman B. Sumitro Sutiman B. Sumitro Sutiman B. Sumitro Sutiman Bambang Sumitro Suwondo, Aris Syahputra Wibowo Teguh Suprihatin Teguh Suprihatin Widodo Widodo Yayu Tsamrotul Fuadah Yoga Dwi Jatmiko Yoga Dwi Jatmiko Yuni Kilawati Zauhani Kusnul