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Journal : Jurnal Agroteknos

EKSPLORASI DAN KARAKTERISASI AZOTOBACTER INDIGENOUS UNTUK PENGEMBANGAN PUPUK HAYATI TANAMAN PADI GOGO LOKAL DI LAHAN MARJINAL ANDI NURMAS; NOFIANTI -; ABDUL RAHMAN; ANDI KHAERUNI
Jurnal Agroteknos Vol 4, No 2 (2014)
Publisher : Jurnal Agroteknos

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Abstract

Azotobacter is bacterium that has the ability to fix nitrogen and produce hormone IAA, thus potentially acts as biofertilizer agent. There are differences in chemical, biological and other characters of Azotobacter strains. Some strains have higher ability to fix nitrogen than the others. Exploration and characterization are important to be done because the bacteria that provide nutrients and live free rhizosphere regions, possible have different abilities.  In addition to N2 fixing, growth hormone production, phosphate dissolving, they are also tolerant to a certain temperature and pH. The purpose of the research was to obtain information and the latest data on indigenous Azotobacter that potential as a source of biofertilizer, and triggering factor for upland rice growth on marginal lands in Southeast Sulawesi.  Exploration from several locations has selected 21 Azotobacter isolates. The test results indicated that the 21 Azotobacter isolates have the ability to produce IAA, dissolve phosphate and stable at pH 5.0-7.0. All 21 isolates tested had the ability to survive at 40oC, eight isolates i.e. LT2D1, LT2d2, LU2c, RG4c, MP1f, LT2d3, ML1j, and RR8awere able to survive at a temperature of 45o C, and LT2d1 isolate survived at temperatures 50oC. The results of the evaluation of the wet weight of upland rice seedlings selected 10 isolates that were: KU6e, MS3e, RG4c, RR8b1, LU2c, RB4b, MS3f, LU2c1, RJ5e, RR8b2 and evaluation of seedling dry weight selected 5 isolates that were: RB4b, LU2c, RJ5e, RR8b2, LT2d1. Keywords:      exploration, characterization, indigenous Azotobacter, local upland rice
ISOLASI DAN KARAKTERISASI BAKTERI MANOLITIKASAL BONGGOL POHON SAGU SRI WAHYUNI; LIANTO -; ANDI KHAERUNI
Jurnal Agroteknos Vol 4, No 3 (2014)
Publisher : Jurnal Agroteknos

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Abstract

Sago Processing in Southeast Sulawesi often generate waste in the form of pulp and tuberswhich contains lignin, cellulose, starch, minerals, and vitamins that can be used as a source of carbon and energy for growth of microorganisms, so it is likely to get microbes, including mannolitic bacteria that are useful for human life. The aim of this study was to isolate and characterize the biochemical properties of mannolitic bacteria originated fromwaste of sago hump in Konawe, Southeast Sulawesi Province. Isolation was done using serial dilution method and then spread over the surface of Nutrient agar medium and Mannan enrichment. Bacterial isolates showing high mannose activity were characterized mannose physiologically and biochemically. From this research, 6 mannolitic isolates originated from hump of sago waste samples from South Konawe were obtained. BLS.11-01 and BLS.11-02 mannolitic bacterial isolates had a strong mannolitic activity, with mannolitic index value of 2.3 and 2.0, respectively. Presumably, the two isolates were gram-positive bacteria, belonging to the same genus.
DETEKSI POTYVIRUS PADA NILAM (Pogostemon Cablin (BLANCO) BENTH) DENGAN TEKNIK ELISA DI SULAWESI TENGGARA MUHAMMAD TAUFIK; ASMAR HASAN; ANDI KHAERUNI; GUSNAWATY HS - -; SARAWA MAMMA
Jurnal Agroteknos Vol 4, No 1 (2014)
Publisher : Jurnal Agroteknos

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Abstract

Mosaic symptoms were observed on Patchouli (Pogostemon cablin) around North Kolaka and Kolaka, Southeast Sulawesi during surveys in early 2012. Indirect-ELISA based detection was conducted using symptomatic leaf samples. The objective of the research was to determine disease incidence of Potyvirus in several farms of Patchouli plant in Southeast Sulawesi. The results showed that Patchouli plant (Pogostemon cablin) was found to be infected with Potyvirus disease showing mosaic symptoms and malformation on the leaf samples i.e. in Amotowo and Boro-Boro Villages of subdistrict Boro-Boro, and Landabaro Village, Mowila subdistrict of South Konawe regency; Asinua Village of subdistrict Unaaha, Lambuya district of subdistrict Lambuya, and Bungguosu district, Konawe subdistrict of Konawe regency; and Anduonohu district, Poasia subdistrict of Kendari regency. This is the first report on Potyvirus infection on patchouli in Southeast Sulawesi. Keywords: Inderect-ELISA, mosaic, Potyvirus, Pogostemon cablin