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Dwi Wahyuni
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Journal : SAINTIFIKA

 1 
The Perbandingan Toksisitas Supernatan dan Endapan Ekstrak Terpurifikasi Daun Mindi (Melia Azedarach L.) terhadap Mortalitas Larva Nyamuk Aedes Aegypti L. Rachmita Mustika Putri; Dwi Wahyuni; Kamalia Fikri
saintifika Vol 24 No 1 (2022)
Publisher : FKIP Universitas Jember

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Abstract

One of the efforts to replace chemical insecticides to eradicate DBD is to obtain natural larvicides and it’s so environmentally friendly. Vegetable insecticides are carried out using a purified extraction process. The purpose of this study was to compare the toxicity of the supernatant and precipitated purified extract of mindi leaf (Melia Azedarach L.) on the mortality of Aedes aegypti L mosquito larvae. The samples used in this study ranged from 600-700 Aedes aegypti L mosquito larvae. This study used a design design. Completely Randomized (CRD) and the Minitab 14 for Windows application help to perform the analysis. The results showed the comparison of the toxicity of the purified extract supernatant of mindi leaf (Melia azedarach L). lower with an LC50 value > 1000 while the toxicity of the precipitated purified extract of mindi leaves (Melia azedarach L). higher with LC50 value < 1000. This research can be further tested regarding the magnitude of the toxicity in this study related to the measurement of environmental factors and also related to the KLT test to determine the possibility of new compounds, which are synergistic in the precipitate (fraksi etanol) and supernatant (fraksi n-heksan) in purified mindi leaf extract (Melia azedarach L).
TOKSISITAS EKSTRAK TERPURIFIKASI DAUN PULUTAN (Urena lobata L.) FRAKSI ETANOL TERHADAP MORTALITAS LARVA NYAMUK Aedes Aegypti Indah Atika Amalia; Dwi Wahyuni; Kamalia Fikri
saintifika Vol 23 No 2 (2021)
Publisher : FKIP Universitas Jember

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Abstract

Dengue hemorrhagic fever (DHF) is a disease that is a major public health problem throughout the world. Indonesia is one of the countries with the largest dengue cases in the world. One of the largest dengue cases in Indonesia is in East Java Province whic h reached 5,733 cases. Dengue fever is transmitted to humans through the Aedes aegypti mosquito infected with the dengue virus. The way to control DHF is to control the vector, namely by breaking the mosquito life cycle using selective and safe biological larvicides. Plants that have potential as biological larvicides are pulutan ( Urena lobata L.) especially the leaves. Pulutan leaves are used as biological larvicides through an extract purification process. This study aims to determine the toxicity of puri fied extract of pulutan leaf ( Urena lobata L.) ethanol fraction on mortality of Aedes aegypti mosquito larvae. Toxicity based on WHO standard (2002), was determined by LC 50 of purified extract of pulutan leaf ethanol fraction on mortality of Aedes aegypti mosquito larvae. Results Based on probit analysis using the Minitab 14 application software, the LC 50 value was 905,36 5 ppm with a lower limit of 810 , 626 ppm and an upper limit o f 992 , 277 ppm. According to this study, purified extract of pulutan leaf ethanol fraction was toxic to Aedes aegypti mosquito larvae and had a larvicidal effect
PERBEDAAN TOKSISITAS EKSTRAK DAUN SIRIH (Piper betle L.) DENGAN EKSTRAK BIJI SRIKAYA (Annona squamosa L.) TERHADAP LARVA NYAMUK Aedes aegypti L. Dwi Wahyuni; Intania Loren
saintifika Vol 17 No 1 (2015)
Publisher : FKIP Universitas Jember

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Abstract

Dengue Fever is a public health problem that is more widespread endemic area for morbidity is high enough into something that must be addressed immediately.The way to solve this desease chemically can make this mosquito becomes resistant. To solve this problem, it needs to find an alternative way in which it can be a natural insecticide that is safe like compounds that comes from Piper betle leaves and Annona squamosa seed. The active compounds of Piper betle leaves that are mixed with the active compounds of Annona squamosa seeds can be used as bioinsecticide. Piper betle leaves contain the active compounds of saponin, alkaloid, flavonoid and tannin, while Annona squamosa seeds contain theactive compounds of annonacin, squamocin, asimisin and alkaloid. This research aimsed to investigate the toxicity of themixture of the Piper betle leaves extract and Annona squamosa seeds againet Aedes aegypti mosquito larvae and tocompare the toxicity of the extract mixture and single extract. The method of this research used complete random design bythree repetitions. The concentratios used were 300, 600, 900, 1200 and 1500 ppm for Piper betle leaves extract and 5, 10, 50, 70 and 100 ppm for Annona squamosa seeds extract with aquades asthe control. The data were analyzed by using probit analysis to determine the LC50. The LC50 score of the Piper betle leaves extract was 910,38 ppm and the LC50 of the Annona squamosa seeds extract was 20,62 ppm. This result showed that toxicity of Piper betle leaves extract with different Annona squamosa seeds which was toxicity is higher than the toxicity of Piper betle leaves extract.
POTENSI ANTAGONIS BAKTERIA SERRATIA MARCESSCENS TERHADAP BAKTERI STREPTOCOCCUS PYOGENESIS DAN BAKTERI VIBRIO CHOLERA SECARA IN VITRO Muhammad Efendi; Dwi Wahyuni; Imam Mudakir
saintifika Vol 18 No 2 (2016)
Publisher : FKIP Universitas Jember

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Abstract

In indonesia, infection diseases are a very high problem among the public, one the common infectious diseases suffered by the Indonesian community is imflamtion of the tonsils caused by Streptococcus pyogenesis and Cholera caused by Vibrio cholera. The use of chemical antibiotics will have a negative impact in health, so necessary alternative replecement of natural antibiotics by Serratia mercescens can pruduce prodigiosin. This reseacrh was to test the potential of Serratia mercescens antagonist against Streptococcus phyogenesis and Vibrio cholera in vitro, using Completely Randomized Design with one treatment factor from Serratia mercescens bacteria concentration. The concentration used was 30μm for each bacterial suspension and added positive control in the form of chloramphenicol and negative control of aquades, each of which was repeated 3 repititions. The results were analyzed using one-way ANOVA and continued D- test. The result showed that Serratia mercescens had blocked inhibitability of Streptococcus phyogenesis bacteria with an average resistance of1.1033mm and on Vibrio cholera with an average resistance of 1.2233mm.
Toksisitas Ekstrak Terpurifikasi dengan N-heksan Buah Kecubung (Datura metel L.) terhadap Mortalitas Larva Nyamuk Aedes aegypti Aditya Rico Armyandi; Dwi Wahyuni; Kamalia Fikri
saintifika Vol 24 No 1 (2022)
Publisher : FKIP Universitas Jember

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Abstract

Infectious diseases caused by animal vectors are currently still a health problem in the world even in Indonesia, especially the Aedes aegypti mosquito which is the vector for the spread of dengue fever. Chemical control is still a priority for the community, such as the use of abate. Continuous use of abate can cause resistance to the Aedes aegypti mosquito to the next generation and pollute water conditions. One of the control solutions can be done with natural insecticides such as amethyst fruit (Datura metel L.). The purpose of making purified extracts is to remove unnecessary impurities and get a larger content of active compounds and increase the concentration of active compounds. The concentration of amethyst extract (Datura metel L.) used was a concentration of 100 ppm, 500 ppm, 1000 ppm, 1500 ppm, 1gr abate as a positive control and aquades as a negative control. The amount of Lethal Concentration-50 (LC50) purified extract of amethyst fruit (Datura metel L.) with n-hexane was 494.987 ppm. The active compound content of amethyst fruit (Datura metel L.) in the n-hexane fraction after a phytochemical screening test was carried out was positive for alkaloids, steroids, terpenoids and tannins. The toxicity of the purified extract with n-hexane of amethyst (Datura metel L.) against Aedes aegypti mosquito larvae within 24 hours is considered toxic because it has an LC50 value of less than 1000 ppm.
Toksisitas Ekstrak Terpurifikasi Daun Buas-Buas (Premna serratifolia L.) terhadap Mortalitas Larva Nyamuk Culex sp. Ulmiyatul Alifiah Zahroh; Dwi Wahyuni; Mochammad Iqbal
saintifika Vol 24 No 1 (2022)
Publisher : FKIP Universitas Jember

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Abstract

Culex sp. is a vector of filariasis. Vector control mostly uses chemical insecticides which have a negative impact. Buas-buas plants (Premna serratifolia L.) are one of the plants that have the potential as an alternative to the use of chemical insecticides that have been widely used (as botanical insecticides). Buas-buas leaves (Premna serratifolia L.) contain alkaloids, flavonoids, saponins, and tannins. This study aimed to test the toxicity of the purified extract of buas-buas leaf (Lethal concentration 50) on the mortality of Culex sp. larvae with an exposure time of 24 hours. Serial concentrations of purified extract used were 850, 1350, 1850, 2350, and 2850 ppm with four repetitions. Each treatment used 20 Culex sp. late three to early four instars. Toxicity testing of Culex sp. gives an LC50 result of 1799.77 ppm..
TOKSISITAS GRANULA EKSTRAK BUAH JERUK NIPIS (Citrus aurantifolia L.) TERHADAP MORTALITAS BERUDU (Phyrinoidis aspera) Renny Ria Fitriani; Dwi Wahyuni; Kamalia Fikri
saintifika Vol 19 No 2 (2017)
Publisher : FKIP Universitas Jember

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Abstract

Granules extract of clime fruit (Citrus aurantifolia L.) is a biolarvasida in eradicating Aedes aegypti mosquito larvae L. Granules extract of lime fruit (Citrus aurantifolia L.) can kill mosquito larvae because its content of limonoida, tannin, flavonoid, and volatile oil. The compound is lethal to the larvae which will enter the body of the larvae of the Aedes aegypti L. mosquito which disrupts the systems in its body. The purpose of this article is to determine the safety of lime fruit (Citrus aurantifolia L.) extract granules on non-target animals, namely tadpoles. Tadpole has the same habitat with mosquito larvae so it is done safety test of citrus fruit extract granule (Citrus aurantifolia L.). Tadpole is a larval phase in Amphibi that continues to experience morphological development in water. The initial phase of tadpoles growth is a phase of adaptation to its habitat. Individuals who can not adapt well will experience a faster death. The research method used is laboratory experimental. The analysis used is ANOVA. The independent variables in this study were the variety of granules extract concentration of citrus fruit (Citrus aurantifolia L.). Tadpole mortality became the dependent variable in this study. Tadpole mortality is characterized by tadpoles collapsing at the time of administration of granules. The results showed that the lime extract granules (Citrus aurantifolia L.) proved safe.
Co-Authors Abd. Rozak Adila Noor Rifa Aditya Rico Armyandi Aditya Rico Armyandi Agustin Syamsianah Armaini Armaini Cahyo Tri Atmojo Denny Adrian Nurhuda Dian Prima Agustina Diemroh Ihsan Dini Aisyafahmi Dwi Arfinanta Edy Soesanto Faizatun Ni’mah Harini Harini Herry Suswanti Djarot Hidayati, Wiwin Sri Iis Nur Asyiah Imam Mudakir Indah Atika Amalia Indah Atika Amalia Indriyani Indriyani Intania Loren John Friadi Kamalia Fikri Khaerani Kusriani Prasetyowati La Ode Nggawu La Ode Sidu Marafat Lina Susilowati Luh Putu Ratna Sundari Mochammad Iqbal Muhammad Efendi Munifah Wahyuddin Noval Alfiyan Jaya Novita Kumalasari Nurida Ulin Ni’mah Nurshalati Tahar Petrus Geroda Beda Ama Petrus Geroda Beda Ama Rabiatul Adawiyah Rachmita Mustika Putri Rachmita Mustika Putri Renny Ria Fitriani Renny Ria Fitriani Risma Karlina Putri Rita Hayati Rizka Sulistianingsih Siti Murdiyah Slamet Hariyadi Sri Pujiati Suciati Sudarisman Sugiyarto Sugiyarto Suryadi Suswadi Trio Ageng Prayitno Ulmiyatul Alifiah Zahroh Ulmiyatul Alifiah Zahroh Wenti Dwi Febriani Yuly Diyan Nur Fajriyah Yuyun Kurniawati YUYUN KURNIAWATI