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Journal : Saintifika

TOKSISITAS GRANULA EKSTRAK BUAH JERUK NIPIS (Citrus aurantifolia L.) TERHADAP MORTALITAS BERUDU (Phyrinoidis aspera) Renny Ria Fitriani; Dwi Wahyuni; Kamalia Fikri
Saintifika Vol 19 No 2 (2017)
Publisher : Jurusan Pendidikan MIPA FKIP Universitas Jember

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Abstract

Abstract: Granules extract of clime fruit (Citrus aurantifolia L.) is a biolarvasida in eradicating Aedes aegypti mosquito larvae L. Granules extract of lime fruit (Citrus aurantifolia L.) can kill mosquito larvae because its content of limonoida, tannin, flavonoid, and volatile oil. The compound is lethal to the larvae which will enter the body of the larvae of the Aedes aegypti L. mosquito which disrupts the systems in its body. The purpose of this article is to determine the safety of lime fruit (Citrus aurantifolia L.) extract granules on non-target animals, namely tadpoles. Tadpole has the same habitat with mosquito larvae so it is done safety test of citrus fruit extract granule (Citrus aurantifolia L.). Tadpole is a larval phase in Amphibi that continues to experience morphological development in water. The initial phase of tadpoles growth is a phase of adaptation to its habitat. Individuals who can not adapt well will experience a faster death. The research method used is laboratory experimental. The analysis used is ANOVA. The independent variables in this study were the variety of granules extract concentration of citrus fruit (Citrus aurantifolia L.). Tadpole mortality became the dependent variable in this study. Tadpole mortality is characterized by tadpoles collapsing at the time of administration of granules. The results showed that the lime extract granules (Citrus aurantifolia L.) proved safe. Keywords: Granules, Mortality, Tadpole
Toksisitas Ekstrak Terpurifikasi dengan N-heksan Buah Kecubung (Datura metel L.) terhadap Mortalitas Larva Nyamuk Aedes aegypti Aditya Rico Armyandi; Dwi Wahyuni; Kamalia Fikri
Saintifika Vol 24 No 1 (2022)
Publisher : Jurusan Pendidikan MIPA FKIP Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/saintifika.v24i1.26579

Abstract

: Infectious diseases caused by animal vectors are currently still a health problem in the world even in Indonesia, especially the Aedes aegypti mosquito which is the vector for the spread of dengue fever. Chemical control is still a priority for the community, such as the use of abate. Continuous use of abate can cause resistance to the Aedes aegypti mosquito to the next generation and pollute water conditions. One of the control solutions can be done with natural insecticides such as amethyst fruit (Datura metel L.). The purpose of making purified extracts is to remove unnecessary impurities and get a larger content of active compounds and increase the concentration of active compounds. The concentration of amethyst extract (Datura metel L.) used was a concentration of 100 ppm, 500 ppm, 1000 ppm, 1500 ppm, 1gr abate as a positive control and aquades as a negative control. The amount of Lethal Concentration-50 (LC50) purified extract of amethyst fruit (Datura metel L.) with n-hexane was 494.987 ppm. The active compound content of amethyst fruit (Datura metel L.) in the n-hexane fraction after a phytochemical screening test was carried out was positive for alkaloids, steroids, terpenoids and tannins. The toxicity of the purified extract with n-hexane of amethyst (Datura metel L.) against Aedes aegypti mosquito larvae within 24 hours is considered toxic because it has an LC50 value of less than 1000 ppm. Kata Kunci: Aedes aegypti; Datura metel L.; n-heksan; Terpurifikasi; Toksisitas
The Perbandingan Toksisitas Supernatan dan Endapan Ekstrak Terpurifikasi Daun Mindi (Melia Azedarach L.) terhadap Mortalitas Larva Nyamuk Aedes Aegypti L. Rachmita Mustika Putri; Dwi Wahyuni; Kamalia Fikri
Saintifika Vol 24 No 1 (2022)
Publisher : Jurusan Pendidikan MIPA FKIP Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/saintifika.v24i1.26749

Abstract

One of the efforts to replace chemical insecticides to eradicate DBD is to obtain natural larvicides and it’s so environmentally friendly. Vegetable insecticides are carried out using a purified extraction process. The purpose of this study was to compare the toxicity of the supernatant and precipitated purified extract of mindi leaf (Melia Azedarach L.) on the mortality of Aedes aegypti L mosquito larvae. The samples used in this study ranged from 600-700 Aedes aegypti L mosquito larvae. This study used a design design. Completely Randomized (CRD) and the Minitab 14 for Windows application help to perform the analysis. The results showed the comparison of the toxicity of the purified extract supernatant of mindi leaf (Melia azedarach L). lower with an LC50 value > 1000 while the toxicity of the precipitated purified extract of mindi leaves (Melia azedarach L). higher with LC50 value < 1000. This research can be further tested regarding the magnitude of the toxicity in this study related to the measurement of environmental factors and also related to the KLT test to determine the possibility of new compounds, which are synergistic in the precipitate (fraksi etanol) and supernatant (fraksi n-heksan) in purified mindi leaf extract (Melia azedarach L).
TOKSISITAS EKSTRAK TERPURIFIKASI DAUN PULUTAN (Urena lobata L.) FRAKSI ETANOL TERHADAP MORTALITAS LARVA NYAMUK Aedes Aegypti Indah Atika Amalia; Dwi Wahyuni; Kamalia Fikri
Saintifika Vol 23 No 2 (2021)
Publisher : Jurusan Pendidikan MIPA FKIP Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/saintifika.v23i2.28799

Abstract

TOKSISITAS EKSTRAK TERPURIFIKASI DAUN BUAS-BUAS (PREMNA SERRATIFOLIA L.) TERHADAP MORTALITAS LARVA NYAMUK CULEX SP. Ulmiyatul Alifiah Zahroh; Dwi Wahyuni; Mochammad Iqbal
Saintifika Vol 24 No 1 (2022)
Publisher : Jurusan Pendidikan MIPA FKIP Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/saintifika.v24i1.26710

Abstract

Culex sp. is a vector of filariasis. Vector control mostly uses chemical insecticides which have a negative impact. Buas-buas plants (Premna serratifolia L.) are one of the plants that have the potential as an alternative to the use of chemical insecticides that have been widely used (as botanical insecticides). Buas-buas leaves (Premna serratifolia L.) contain alkaloids, flavonoids, saponins, and tannins. This study aimed to test the toxicity of the purified extract of buas-buas leaf (Lethal concentration 50) on the mortality of Culex sp. larvae with an exposure time of 24 hours. Serial concentrations of purified extract used were 850, 1350, 1850, 2350, and 2850 ppm with four repetitions. Each treatment used 20 Culex sp. late three to early four instars. Toxicity testing of Culex sp. gives an LC50 result of 1799.77 ppm.. Kata Kunci: Culex sp.; Premna serratifolia L. ; Terpurifikasi; Toksisitas