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INDONESIA
Biosaintifika: Journal of Biology & Biology Education
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 19 Documents
Search results for , issue " Vol 8, No 3 (2016): December 2016" : 19 Documents clear
Effect on Polysaccharide Krestin from Coriolus versicolor Extract on Phagocytic Activity and Capacity of Mus musculus Exposed by Pseudomonas aeruginosa Wahyuningsih, Sri Puji Astuti; Savira, Nadyatul Ilma Indah; Darmanto, Win
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6957

Abstract

Pseudomonas aeruginosa is an opportunistic bacterium that causes infections in human. The wall cell of its bacteria contains lipopolysaccharide as virulency factors to protect it from human immunity. Lipopolysaccharide can inhibit phagocytosis in the body. Polysaccharide krestin (PSK) from Coriolus versicolor extract contains ?-glucan that can increase phagocytic activity and capacity. This research aimed to identify the effect on polysaccharide krestin from C. versicolor extract on phagocytic activity and capacity of mice exposed by P. aeruginosa. The design of this research was experimental design. There were six treatment groups. The phagocytic activity and capacity were counted on slide smears of mice peritoneal fluid. The data was analyzed by using one way ANOVA. The results of the phagocytic activity and capacity showed that PSK was added before exposure (P1) or after exposure (P2) or before-after exposure (P3) had potential to increase phagocytic activity and capacity. The conclusion of the research was that adding polysaccharide krestin either before exposure or after exposure or both of them could increase phagocytic activity and capacity. The benefits of this research to development of science are expected to reduce human infection and to utilize natural ingredients as immunomodulator.How to CiteWahyuningsih, S. P. A., Savira, N. I. I, & Darmanto, W. (2016). Effect on Polysaccharide Krestin from Coriolus versicolor Extract on Phagocytic Activity and Capacity of Mus musculus Exposed by Pseudomonas aeruginosa. Biosaintifika: Journal of Biology & Biology Education, 8(3), 308-313.
Agronomic Characterization of Wheat Mutants (Triticum aestivum) of M3 Generation Planted in Sukabumi Sari, Laela; Purwito, Agus; Sopandie, Didy; Purnamaningsih, Ragapadmi; Sudarmonowati, Enny
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6612

Abstract

The purpose of this study was to identify the selection criteria to obtain a superior mutant derived from the wheat plants of such varieties as Dewata, Selayar and Alibey, adaptive in medium land. The analysis of agronomic growth characters showed a significantly effect on a growth percentage of the initial growth (8 mutants), flowering time (1 mutant), panicle stem length (15 mutants), number of panicles (7 mutants), the number of grains per panicle (8 mutants), grain weight observed (8 mutants), grain weight per genotype (6 mutants), leaf area (2 mutants) and leaf greenness (5 mutants). The effects on the characters of ripe time, harvest, panicle length and plant height were not significant. The mutants of Dewata, Selayar and Alibey could be selected based on the characters of panicle stem length, number of grains per panicle and grain weight per observation because these characters generated more mutants than the other characters. The correlation analysis between the characters of growth and yield components of wheat mutants showed that the number of grains per panicle was positively correlated with the grain weight observed, while the length of panicle stem was positively correlated with grain weight per genotype, number of panicles and leaf area. Hopefully some mutants produced could adapt to the tropical medium land, thus adding to the diversity of wheat germplasm in Indonesia, thereby reducing the import of wheat to Indonesia.How to CiteSari, L., Purwito, A., Sopandie, D., Purnamaningsih, R. & Sudarmonowati, E. (2016). Agronomic Characterization of Wheat Mutants (Triticum aestivum) of M3 Generation Planted in Sukabumi. Biosaintifika: Journal of Biology & Biology Education, 8(3), 353-361.
Phylogenetic Relationships of Local Durian Species based on Morphological Characteristics and PCR-RFLP Analysis of the Ribosomal Internal Transcribed Spacer (ITS) DNA Fibriana, Fidia; Hadiyanti, Lutfia Nur
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6748

Abstract

In this study, twenty local durian accessions obtained from Central Java in situ collection were characterized using the morphological characteristics and the restriction patterns which generated from the region spanning the internal transcribed spacers ITS LEU and ITS 4. Morphological characteristics of durian leaf, stem, tree, and fruit showed variations for the different accessions, whereas polymerase chain reaction (PCR) products of ribosomal DNA region showed a low length of variation. The size of the PCR products and the restriction analyses with the restriction endonucleases Bsp1431yielded a restriction pattern for each accessions. The results of this study can be utilized by local durian farmers as a preliminary reference for durian propagation. The data obtained need to be supported by further research using the other molecular markers to obtain more accurate data. The clear identity of durian species can help the management of propagation systems by farmers to get superior local durian.How to CiteFibriana, F., & Hadiyanti, L. N. (2016). Phylogenetic Relationships of Local Durian Species based on Morphological Characteristics and PCR-RFLP Analysis of the Ribosomal Internal Transcribed Spacer (ITS) DNA. Biosaintifika: Journal of Biology & Biology Education, 8(3), 362-370.
Immune Responses of Wistar Rat (Rattus novergicus) on Adduction of Humid Acid from Borneo Peat Soil Rousdy, Diah Wulandari; Rahmawati, Rahmawati; Kurniatuhadi, Rikhsan
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.7499

Abstract

Peat soil is a type of soil that dominates the island of Borneo. Typical compounds in peat soil is humic acid. Various in vitro studies performed have shown peat subtropical humic compounds can stimulate the immune system. However, in vivo study on animal has not been done. This study aimed to determine the effect of humic acid extracted from peat soil of Borneo against the immune system, both of non-specific and specific immunity Wistar rats (Rattus novergicus). Research using a completely randomized design with five treatments and five replicates, the normal controls, a positive control (isoprinosine), humic acid 125; 250; 500 mg/kg. Humic acid was administered orally for 10 days. The results showed humic acid adduction did not significantly affect levels of hemoglobin, erythrocytes and hematocrit. Humic acid adduction of 125 mg/kg significantly affects the total leukocyte count and differential leukocyte. Humic acid 125 mg/kg also showed increased phagocytic index better than normal controls. All humic acid treatments do not provide a significant effect on the total amount of antibody. The results of this study can be used for the development of Borneo tropical peat resources as natural imunostimulant.How to CiteRousdy, D. W., Rahmawati, R. & Kurniatuhadi, R. (2016). Immune Responses of Wistar Rat (Rattus novergicus) on Adduction of Humid Acid from Borneo Peat Soil. Biosaintifika: Journal of Biology & Biology Education, 8(3), 401-406.
Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (Hermetia illucens) Feeding with Rice Straw Supriyatna, Ateng; Ukit, Ukit
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6762

Abstract

A research on screening and isolation of cellulolytic bacteria from the gut of larvae Black Soldier treated rice straw feed has been conducted. The purpose of this study is to get the type of cellulolytic bacteria from the gut of larvae and bacteria that have the highest potential to degrade cellulose. Screening and isolation method applied by using intestinal larvae obtained from larval gut vortex at a speed of 1500 rpm. Furthermore, dilution graded from 1 to 10 and grown in media CMC (carboxyl methyl cellulose) at 37 0C and incubated for 48 hours. Observations were made based on the characteristics of the microscopic, macroscopic, biochemical test, cellulolytic activity and the activity of cellulase enzymes selected bacteria. The results showed a 9 cellulolytic bacteria from the gut of the larvae. Bacillus sp. is a bacteria that have the highest potential with cellulolitic activity 2.1 mm (dz/dk), the exponential phase of hour at the 24th, and cellulase enzyme activity of 0.4 U/mL at pH 7 and 0.41 U/mL at pH 8.This research showed that the Black Soldier Flays Larvae (Hermetia illucens) have competencein organic waste degradation, because in Black Soldier Flays Larvaes gut, cellulolitic enzyme is produced by cellulolitic bacteria, specially Bacillus sp,How to CiteSupriyatna, A., & Ukit, U. (2016). Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (Hermetia illucens) Feeding with Rice Straw. Biosaintifika: Journal of Biology & Biology Education, 8(3), 314-320.
Relationship between Zingiberaceae Leaves Compounds and its Tyrosinase Activity Batubara, Irmanida; Kartika, Yuni; Darusman, Latifah K
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6742

Abstract

The leaves of Zingiberaceae family has not been much explored its potential, especially as a skin lightening. The relationship between total anthocyanins, chlorophyll, carotenoids and tannins contents of Zingiberaceae leaves and tyrosinase activity were determined. Ten species of Zingiberaceae were separated by n-hexane and the residues were extracted with ethyl acetate. The total anthocyanins, chlorophyll, carotenoids, tannins contents and the activities of ethyl acetate extracts were determined by spectrometric method. The tyrosinase inhibition was determined by using L-tyrosine (monophenolase) and L-DOPA (diphenolase) substrates. The most active extract was the leaves extract of Zingiber purpureum which exhibited 82.86% for monophenolase inhibition and the leaves extract of Curcuma zedoaria which exhibited 90.20% for diphenolase inhibition. The correlation between carotenoids content and monophenolase inhibition was 52%, while between tannin content and diphenolase inhibition was only 15%. Therefore, among 10 leaves species of Zingiberaceae, Zingiber purpureum Roscoe and Curcuma zedoaria leaves are the most potential for tyrosinase inhibitors and can be developed as whitening agent.How to CiteBatubara, I., Kartika, Y. & Darusman, L. K. (2016). Relationship between Zingiberaceae Leaves Compounds and its Tyrosinase Activity. Biosaintifika: Journal of Biology & Biology Education, 8(3), 371-377.
Effects of Some Feed Suplements Types to the Growth of Javaen Barb/Brek Fish (Puntius orphoides) Second Filial as Domestication Product Susatyo, Priyo; Sugiharto, Sugiharto; Hana, Hana; Chasanah, Titi
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.5517

Abstract

Aquaculture can be improved by a diversified approach in the form of domestication research of a wild fish. The research has been done by cultivating Javaen Barb/Brek (Puntius orphoides) from Serayu river, Banyumas at a natural pond and laboratory scale. Brek fish is relative slow in growth and development of gonad. This research is aimed to determine: (1) the rate of general growth of Brek in the provision of some kinds of additional food; (2) Relative Growth Rate (RGR); (3) Specific Growth Rate (SGR) and Feed Conversion (FC). The research used experimental methods. The method was a completely randomized design (CRD) of 4 treatments and 3 replicationsof: A = 100% pellet; B = 70% soybean sprout and 30% pellet; C = 70% Ipomoea aquatica leaf and 30% pellet; D = 35% soybean sprouts, 35% I. aquatica leaf and 30% pellet. Quantitative data were analysed by analysis of variance (ANOVA) of the fishes growth.The results show influence of feed types to the Relative Growth Rate (RGR) of Javaean Barb. The best RGR with the value of 72.40 was given by feed types in the D combination. Meanwhile, Specific Growth Rate (SGR) and Feed Conversion (FC) were not affected by the feed types. The study were expected to provide suggestions for efficient feeding techniques in attempts at domestication of wild fish.How to CiteSusatyo, P., Sugiharto, S., Hana, H., & Chasanah, T. (2016). Effects of Some Feed Suplements Types to The Growth of Javaen Barb/Brek Fish (Puntius orphoides) Second Filial As Domestication Product. Biosaintifika: Journal of Biology & Biology Education, 8(3), 278-285.
Identification of Soybean Resistance to Pod Sucking Bug (Riptortus linearis) by No-Choice Test Krisnawati, Ayda; Bayu, Marida Santi Yudha Ika; Adie, Moch. Muchlish
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.5180

Abstract

Soybean pod damaged by pod sucking bug (Riptortus linearis) is one of the constraint within soybean yield improvement in Indonesia. The research aimed was to identify the resistance of soybean genotypes to pod sucking pest. The experiment was conducted in Iletris screen house from March to June 2015. The experiment was arranged in Randomized Completely Block Design using 10 soybean genotypes with three replicates. The soybean resistance was evaluated using no-choice test. Data collected on number of pod/plant, number of seed/plant, seed weight per plant, number of attacked pod/plant, and number of attacked seed/plant. The result showed that the lowest percentage both of pod and seed damage was G511H/Anjasmoro//Anjasmoro-2-8, that was 25.83 % and 19.12 %, respectively. Based on the value of the percentage of seed damage, there were five susceptible genotypes, three moderately resistant, and two resistant genotypes. Based on the value of the percentage of pod damage, showed four susceptible genotypes, five moderately resistant, and a resistant genotype. G511H/Anjasmoro//Anjasmoro-2-8 was the only resistant genotype, and it could be used as a genetic source in the improvement of soybean resistance to pod sucking bug.How to CiteKrisnawati, A., Bayu, M. S. Y. I. & Adie, M. M. (2016). Identification of Soybean Resistance to Pod Sucking Bug (Riptortus linearis) by No-Choice Test. Biosaintifika: Journal of Biology & Biology Education, 8(3), 407-414.
Population Dynamics of Pometia for The Period of Post-Selective Logging in Tropical Rainforest, Southern Papua, Indonesia Murdjoko, Agustinus; Marsono, Djoko; Sadono, Ronggo; Hadisusanto, Suwarno
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6309

Abstract

Tropical rainforest changed in term of structure, composition and population, resulting from logging. One of target trees during logging is Pometia. Our goals of this research were to answer that (1) How were survival, growth, fecundity and population growth rate (?) of Pometia within remnant stands?; (2) what were driving factors to explain population dynamics of Pometia within logged forest?. Data were derived from three hectares-permanent sample plot (PSP) in logged forest of PT Tunas Timber Lestari. Integral Projection Models (IPMs) were applied to analyze population dynamics. The study showed dynamically that probability of the individuals of Pometia survived in this area did not significantly differ among their size, while stands showed the significant difference between small and large individuals. The growth of both individuals of Pometia and stands varied significantly in different size. The probability of fecundity both Pometia and stands were significantly different among size. The population of Pometia has been increasing for about a decade as the population of all stands was growing as well. Moreover, the regular documentation of species composition and structure before and after logging is necessary as a control of biodiversity. Therefore, a proper protocol to monitor the biodiversity should be designed by government and disseminated it to logging companies. How to CiteMurdjoko, A., Marsono, D., Sadono, R. & Hadisusanto, S. (2016). Population Dynamics of Pometia for The Period of Post-Selective Logging in Tropical Rainforest, Southern Papua, Indonesia. Biosaintifika: Journal of Biology & Biology Education, 8(3), 321-330.
Species Richness and Birds Conservation Priority in Mount Rinjani Areas, Lombok Hadiprayitno, Gito; Mertha, I Gde; Ilhamdi, Moh. Liwa
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.5039

Abstract

Mount Rinjani area on the island of Lombok is unique in bioecology. It provides habitats for many species of birds, especially birds of Wallacea. Several studies on birds in this area have been done, but the conservation priority of these birds has not been determined. Based on this condition, the purpose of this research is to determine the priority of birds conservation in mount Rinjani areas. Exploration about the spesies of birds was started from Senaru Village to Segara Anak Lake. Abundance of each bird species was estimated using point count. Data analysis to determine the conservation priority used indicators that consist of endemicity, population status, species threatened and species management. The results showed that the birds species in mount Rinjani areas are composed of 32 species belonging to 20 families. Bird species that have high abundance were Amandava amandava (15.94%), Zosterops Montanus (15.70%), and Parus major (11.32%), while Ptilinopus melanospila and Anthus novaeseelandiae were in low abundance (0.23%). Analysis birds conservation priority in mount Rinjani areas indicates that Otus jolandae has the highest score (65) and should be the first priority for the conservation, while Philemon buceroides and Gallus varius with the same score (60) are the second priority. How to CiteHadiprayitno, G., Mertha, I. G., & Ilhamdi, M. L. (2016). Species Richness and Birds Conservation Priority in Mount Rinjani Areas. Biosaintifika: Journal of Biology & Biology Education, 8(3), 270-277.

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