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Flavanoids fromthe Stembark of Chisocheton pentandrus(Meliaceae) Supriatno Supriatno; Ace Tatang Hidayat; Kindi Farabi; Fajar Fauzi Abdullah; Nurlelasari Nurlelasari; Tati Herlina; Unang Supratman; Khadijah Awang
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 3, No. 2, November 2017
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (558.628 KB) | DOI: 10.15408/jkv.v0i0.6077

Abstract

Two flavanoid compounds, catechin (1) and epicatechin (2), have been isolated from the stembark of Chisocheton pentandrus. The chemical structure of compounds1and2were identified byspectroscopic data including, UV, IR, NMR (1H, 13C, DEPT 135°, HMQC, HMBC, 1H-1H COSY) and MS and by comparing with previously reported spectral data. Compounds 1 and 2, were isolated in this plant for first time and showed no cytotoxic activity against MCF-7 breast cancer cells.
Senyawa Bufadienolida yang bersifat Insektisida, Daigremontianin dari daun Cocor Bebek (Kalanchoe daigremontiana) Tri Mayanti; Desi Harneti Putri Huspa; Nurlelasari Nurlelasari; Agus Safari; Unang Supratman
Jurnal Kimia Valensi Jurnal Valensi Volume 2, No.2, Mei 2011
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (184.131 KB) | DOI: 10.15408/jkv.v2i2.200

Abstract

Dalam penelitian berkelanjutan untuk pencarian senyawa-senyawa yang bersifat insektisida daritanaman Kalanchoe (Cocor bebek) Indonesia, diperoleh hasil bahwa ekstrak metanol daun Cocorbebek (Kalanchoe daigremontiana) memberikan aktivitas insektisida yang kuat terhadap instar ke tigalarva ulat sutera (Bombyx mori). Ekstrak metanol yang diperoleh selanjutnya dipisahkan senyawasenyawanyamenggunakan partisi dengan pelarut organik dan kombinasi kolom kromatografi padasilika gel dan ODS sehingga dihasilkan satu senyawa aktif insektisida. Struktur kimia senyawa yangbersifat insektisida tersebut ditentukan dengan metoda spektroskopi dan perbandingan data spektrasenyawa analog yang telah diperoleh dari penelitian sebelumnya sehingga senyawa aktif insektisidatersebut diidentifikasikan sebagai senyawa turunan bufadienolida, daigremontianin. Daegremontianinmenunjukkan aktivitas insektisidal yang kuat terhadap instar ke tiga ulat sutera (Bombyx mori) denganLD50 0,9 μg/g diet.
Cytotoxic Steroids From The Stembak of Chisocheton celebicus KOORD Dewa Gede Katja; Desi Harneti; Tri Mayanti; Nurlelasari Nurlelasari; Rani Maharani; Yoshihito Shiono; Unang Supratman
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 5, No. 2, November 2019
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.577 KB) | DOI: 10.15408/jkv.v5i2.12132

Abstract

In the course of our continuing search for anticancer compounds from Chisocheton species, three steroids, stigmast-5-en-3β-ol (1), stigmast-5-en-3β-ol-3-O-β-D-glucopyranoside (2) and stigmast-5,22-dien-3β-ol-3-O-β-D-glucopyranoside (3), were obtained from the stembark of Chisocheton celebicus. The structures of compound 1-3 were identified with spectroscopic data including IR, 1D-NMR, 2D-NMR and TOF-MS, as well as by comparing with those spectral data previously. Compounds 1-3, were evaluated for their cytotoxic effects against P-388 murine leukemia cells and displayed the cytotoxicity activity with IC50 values of 12.45 ± 0.050, 52.27 ± 0.031 and 62.52 ± 0.076 µg/mL, respectively.
Dammarane-Type Triterpenoids from The Stembark of Aglaia argentea (Meliaceae) Ace Tatang Hidayat; Kindi Farabi; Ida Nur Farida; Kansy Haikal; Nurlelasari Nurlelasari; Desi Harneti Putri Huspa; Rani Maharani; Unang Supratman; Yoshihito Shiono
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 4, No. 1, Mei 2018
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (111.204 KB) | DOI: 10.15408/jkv.v4i1.7065

Abstract

Two dammarane-type triterpenoids, 20S,24S-epoxy-3α,25-dihydroxydammarane (1) and 3α-acetyl-20S,24S-epoxy-3α,25-dihydroxydammarane (2), have been isolated from the stembark of Aglaia argentea. The chemical structure of compounds (1 and 2) were identified by spectroscopic evidences including UV, IR, 1D-NMR, 2D-NMR and MS as well as by comparing with previously reported spectral data. Those compounds were isolated from this plant for first time. Compounds (1 and 2) showed cytotoxic activity against P-388 murine leukemia cells with IC50 values of 23.96 and 8.14 mM, respectively.DOI:http://dx.doi.org/10.15408/jkv.v4i1.7065
Molecular Docking on Kokosanolide A and C for Anticancer Activity Against Human Breast Cancer Cell MCF-7 Sri Purwani; Julita Nahar; Zulfikar Zulfikar; Nurlelasari Nurlelasari; Tri Mayanti
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 7, No. 1, May 2021
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v7i1.20534

Abstract

Kokosanolide A (1), from the seeds of Lansium domesticum Corr. cv Kokossan, has been shown strong cytotoxic activities (IC50 = 8.62 μg/mL) against MCF-7 breast cancer cells. The aim of this work was to study the molecular interactions of kokosanolide A and kokosanolide C with the Estrogen Receptor α (ERα) using computer-aided drug design approaches. Molecular docking using Autodock Vina (open-source software PyRx 0.8) was employed to explore the modes of binding of kokosanolide A (1) and kokosanolide C (2) with ERα. Compounds 1 and 2 showed strong bond-free energy (-8.8 kcal/mol and -8.7 kcal/mol) to ERα. These two compounds have a molecular mechanism to inhibit ERα in breast cancer cells.
Sintesis Tetrapeptida PADY menggunakan Metode Fasa Padat dan Aktivitas Antioksidannya Rani Maharani Ph.D; Dadan Sumiarsa; Christina Marpaung; Achmad Zainuddin; Ace Tatang Hidayat; Desi Harneti; Nurlelasari Nurlelasari; Unang Supratman
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 5, No. 1, May 2019
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (845.195 KB) | DOI: 10.15408/jkv.v5i1.10500

Abstract

Peptida antioksidan merupakan kelompok peptida yang berperan penting karena dapat menetralkan radikal bebas, sehingga dapat mencegah dan mengobati penyakit kronis. Salah satu senyawa peptida antioksidan alami yang telah ditemukan peneliti sebelumnya adalah senyawa tetrapeptida PAGY (Pro-Ala-Gly-Tyr) yang diisolasi dari gelatin kulit ikan amur sturgeon (Acipenser schrenckii) dan dilaporkan memiliki aktivitas antioksidannya dengan IC50 5.38 mg/mL dalam uji DPPH dan 0,008 mg/mL dalam uji ABTS berturut-turut. Kelompok kami telah berhasil mensintesis PAGY bersama dengan analognya yakni PSGY, PFFY, PAFY, dan PAIY dengan menggunakan metode sintesis peptida fase padat (SPPS). Pengujian aktivitas antioksidan pada senyawa hasil sintesis menunjukkan bahwa PSGY memiliki aktivitas antioksidan lebih baik dari PAGY. Pencarian analog tetrapeptida antioksidan yang lebih baik hingga saat ini masih terus dilakukan. Pada penelitian ini telah berhasil disintesis analog tetrapeptida lainnya PADY (Pro-Ala-Asp-Tyr) dengan metode sintesis peptida fase padat menggunakan strategi Fmoc/t-Bu pada resin 2-klorotritilklorida dilanjutkan dengan pengujian aktivitas antioksidannya. HATU/HOAT digunakan sebagai reagen pengkopling dalam sintesis PADY. Pemurnian krud PADY dilakukan menggunakan RP-HPLC preparatif sehingga diperoleh PADY murni seberat 14.7 mg (12.6%). Penentuan struktur peptida hasil sintesis dianalisis dengan menggunakan spektroskopi 1H-NMR dan TOF-MS. Pada pengujian aktivitas antioksidan dengan metode DPPH, PADY hasil sintesis memberikan nilai IC50 sebesar 1.850 mg/mL, yang mengindikasikan bahwa PADY menunjukkan aktivitas antioksidan yang lebih rendah daripada PAGY hasil sintesis peneliti sebelumnya. Kata kunci: Antioksidan, tetrapeptida, sintesis peptida fase padat. Antioxidant peptide is a class of peptides that play an important in neutralizing free radicals, therefore this compound can be used to prevent and treat chronic diseases. One of the natural antioxidant peptides reported by previous researcher is PAGY (Pro-Ala-Gly-Tyr), which is isolated from amur sturgeon fish (Acipenser schrenckii) gelatin that showed antioxidant activity with IC50 5.38 and 0.008 mg/mL using DPPH and ABTS assay, respectively. Our group has successfully synthesized PAGY, along with its analogues of PSGY, PFFY, PAFY, and PAIY using solid phase peptide synthesis method (SPPS). Antioxidant assay on synthesised compounds showed that PSGY has better antioxidant activity than PAGY. The search on the analogues of the antioxidant tetrapeptide was continued. From this study, a tetrapeptide analogue PADY (Pro-Ala-Asp-Tyr) has been successfully synthesised by solid phase peptide synthesis method with Fmoc/t-Bu strategy on 2-chlorotrityl chloride resin and tested for its antioxidant activity. HATU and HOAt reagents were used as the coupling reagent for the synthesis of PADY. The resulting PADY peptide solid was then purified using preparative RP-HPLC yielding PADY of 14.7 mg (12.6%). Characterisation of the synthesized compound was analysed by  1H-NMR and TOF-MS. On the antioxidant assay using DPPH method, PADY showed IC50 value of 1.850 mg/mLindicating a lower activity than the synthetic PAGY. Keywords: Antioxidant, tetrapeptide, solid phase peptide synthesis (SPPS).
STUDI EKSTRAK n-HEKSANA DARI KULIT BATANG KANDIS HUTAN (Garcinia cymosa) Darwati Darwati; Nurlelasari Nurlelasari; Tri Mayanti; Unang Supratman
Jurnal Penelitian Hasil Hutan Vol 39, No 3 (2021): Jurnal Penelitian Hasil Hutan
Publisher : Pusat Penelitian dan Pengembangan Hasil Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jphh.2021.39.3.148-154

Abstract

Tanaman dari Garcinia (Famili Guttiferae) yang tumbuh di hutan tropis Indonesia memiliki potensi kandungan senyawa triterpenoid. Garcinia cymosa telah dilaporkan memiliki potensi sebagai sumber utama senyawa triterpenoid yang  memiliki aktivitas biologis yang bermanfaat, seperti anti-inflamasi, antibakteri, antijamur, anti-oksidan, sitotoksik, dan anti-HIV. Saat ini data dan informasi mengenai senyawa triterpenoid yang terkandung di dalam G. cymosa relatif masih sangat sedikit. Tulisan ini mempelajari senyawa triterpenoid dari kulit batang G. cymosa. Kulit batang G. cymosa dimaserasi dengan menggunakan pelarut n-heksana yang kemudian dipisahkan dan dimurnikan dengan metode kromatografi hingga diperoleh isolat murni berupa kristal jarum putih (10,8 mg). Struktur kimia isolat murni ditentukan dengan  metode spektroskopi IR, 1D-NMR, 2D-NMR dan spektroskopi massa serta dilakukan  perbandingan dengan literatur. Hasil penelitian menunjukkan senyawa friedelin berhasildiisolasi dari n-heksana kulit batang G. cymosa. Senyawa yang telah diisolasi teridentifikasi sebagai senyawa friedelin.
SENYAWA STEROID DARI AKAR TUMBUHAN ASAM KANDIS (Garcinia Cowa) SEBAGAI OBAT PENURUN DEMAM Darwati Darwati; Nurlelasari Nurlelasari; Tri Mayanti
Jurnal Penelitian Hasil Hutan Vol 37, No 1 (2019): Jurnal Penelitian Hasil Hutan
Publisher : Pusat Penelitian dan Pengembangan Hasil Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1682.56 KB) | DOI: 10.20886/jphh.2019.37.1.51-57

Abstract

Garcinia cowa termasuk famili Guttiferae, yang dikenal di Indonesia dengan nama asam kandis. Secara tradisional kulit batang Garcinia cowa telah digunakan sebagai antipiretik dan antimikroba, sementara buah dan daunnya untuk memperlancar peredaran darah, ekspektoran, pencahar, serta akarnya untuk menurunkan demam. Keragaman manfaat tumbuhan Garcinia sebagai obat tradisional tersebut terkait dengan kandungan kimianya. Dalam rangka investigasi berkelanjutan untuk mencari senyawa metabolit sekunder dari tumbuhan genus Garcinia asal Indonesia, suatu senyawa steroid, stigmasterol telah berhasil diisolasi dari bagian akar Garcinia cowa. Isolasi dilakukan dengan cara ekstraksi dan berbagai metode kromatografi. Penentuan struktur senyawa stigmasterol l berdasarkan data spektroskopi NMR dan dengan membandingkan data senyawa yang diperoleh dengan literatur. Berdasarkan analisa spektroskopi disimpulkan senyawa hasil isolasi adalah stigmasterol. Senyawa ini baru dilaporkan dari spesies ini.
Senyawa Disobinin yang Bersifat Antimalaria dari Biji Tumbuhan Chisocheton macrophyllus (Meliaceae) Nurlelasari Nurlelasari; Desi Harneti PH; Unang Supratman
Al-Kimia Vol 4 No 2 (2016): December
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (247.435 KB) | DOI: 10.24252/al-kimia.v4i2.1675

Abstract

Disobinin compounds have been isolated from the seeds Chisocheton macrophyllus (Meliaceae). The chemical structure of compounds disobinin identified based on data covering UV spectroscopy, IR, 1D-NMR, 2D-NMR and mass spectra as well as the comparison with the data obtained from the literature. Disobinin compound showed antimalarial effect against P. falcifarum with IC50 values from 0.001 to 0.01 mg/mL . Limonoids compound, disobinin reported for the first time in the genus of Chisocheton.
Steroid dari Kulit Batang Aglaia grandis (Meliaceae) Kindi Farabi; Nurlelasari Nurlelasari; Siti Hani Pratiwi; Desi Harneti; Rani Maharani; Agus Safari; Unang Supratman
Al-Kimia Vol 9 No 1 (2021): JUNE
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/al-kimia.v9i1.17936

Abstract

Steroids are a group of secondary metabolite which showed various biological activities. Meliaceae is a plant family used as a one of the source of steroid compounds. A. grandis is a member of the Meliaceae family in genus Aglaia which rarely explored. Previous studies have shown that A. grandis leaves contain triterpenoids, steroids, bisamide, and sesquiterpenoids. This study aims to obtain steroids from the bark of A. grandis. The structure of the isolated compound was determined using the NMR 1D and 2 D spectroscopic. Based on the extensive analysis, isolated compound identified as a stigmastant type steroid, stigmast-5-en-3β-ol-7-one.
Co-Authors A. T. Hidayat1 Ace Tatang Hidayat Ace Tatang Hidayat Achmad Zainuddin Agus Safari Agus Safari Agus Safari Al Arofatus Naini Alya Tsamrotul Anastasya Firdausi Andi Rahim Anggit Kartikasari Awang, K. - Christina Marpaung D. D. Harneti D. Darneti D. G. Katja D. Harneti D. Sumiarsa Dadan Sumiarsa Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati Darwati, Darwati Desi Harnet Desi Harneti Putri Huspa Dessy Yulyani Kurnia Dewa Gede Katja Dewa Gede Katja E. Julaeha Erina Hilmayanti Euis Julaeha Euis Julaeha Fajar Fauzi Abdullah Fajar Fauzi Abdullah Ghina Izdihar Harneti, D - Herdanu Rizqullah Hersa Milawati Hersa Milawati Ida Nur Farida Ida Nurfarida Intan Rahmayanti Iqbal Wahyu Mustaqim Iryanto, Y. - J. Sianturi Jamaludin Al-Anshori Julaeha, E. - Julita Nahar, Julita K. K. Farabi K. K. Haikal Kansy Haikal Kansy Haikal Khadijah Awang Khalijah Awang Khalijah Awang Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi Kindi Farabi M. M. Muhammad Mayanti, T. - Moelyono Moektiwardoyo Mohamad Fajar Mohamad Nurul Azmi Mohamad Nurul Azmi Mohamad Nurul Azmi Muflihah, L.F - Muhammad Hanafi Muhammad Hanafi Nadya Thufaila Nita Ike Dwi Kurniasih Nur Muhammad Miftah Nurabi Ferdiana Purnama Purnama Puspa, H - R. Maharani R. Maharani R. R. Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Rani Maharani Ph.D Rizky Abdullah Ronauli Fitriana Russiska Russiska S. Salam S. Soidah Sabarudin, L. - Safari, A - Safri Ishmayana Shabarni Gaffar Siska Mulya Octavia Siska Mulya Octavia Siti Hani Pratiwi Sofa Fajriah Sri Purwani Subekti Mauluddin Supriatno Salam Supriatno Supriatno T. Mayanti Tati Herlina Tati Herlina Tati Herlina Tiara Prima Amalya Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti Tri Mayanti U. Supratman U. U. Supratman Unang Supratman Unang Supratman Wardoyo, M.M - Winda Sukmawati Y. P. Apriantini Y. Shiono Y. Y. Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Yoshihito Shiono Zulfikar Zulfikar