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Fatimawali .
Universitas Sam Ratulangi
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Journal : YARSI Medical Journal

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POPULASI BAKTERI PADA TANAH BEKAS BUANGAN LIMBAH MERKURI TAMBANG EMAS DI KABUPATEN BOLAANG MONGONDOW: PENELITIAN PENDAHULUAN Fatimawali, Fatimawali; Kepel, Billy; Yusuf, Irawan; Natsir, Rosdiana; Baharuddin, Fatmawaty
Jurnal Kedokteran YARSI Vol 17, No 2 (2009): MEI - AGUSTUS 2009
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.068 KB) | DOI: 10.33476/jky.v17i2.207

Abstract

Mercury is one of the most toxic heavy metals found in nature. Athough adverse health effect of mercury have been known for a long time, exposure to mercury continues and is even increasing in some areas, for example, mercury is still used in gold mining in many parts of North Sulawesi Province. Most of the soil and aquatic bacteria that are continuously exposed to mercury usually develop a genetic adaptation to resist the toxicity of this compound. Bacteria have a specific operon called merOperon that functions to coordinate genes coding for proteins and enzymes involved in mercury disposal and detoxification. Therefore, this preliminary study aims to isolate and identify bacteria collected from gold mining area in the district of Bolaang Mongondow. Bacteria were isolated from soil samples collected from three locations of the gold mining waste disposal and the isolated bacteria were grown in agar media. Identification of the grown bacteria were then be performed using morphological, physiological and biochemical tests. The results showed that 36 bacteria were successfully isolated, of which, 11 isolates were gram positive bacteria and the remainders were gram negative. All isolates showed motility and all could be grouped into 4 species i.e. Bacillus sp., Escherichia coli, Enterobacter cloacea, and Enterobacter aerogenes.
PENENTUAN JENIS DENGAN ANALISIS GEN 16SRRNA DAN UJI DAYA REDUKSI BAKTERI RESISTEN MERKURI YANG DIISOLASI DARI FESES PASIEN DENGAN TAMBALAN AMALGAM MERKURI DI PUSKESMAS BAHU MANADO Umar, Fatimawali
Jurnal Kedokteran YARSI Vol 23, No 1 (2015): JANUARI - APRIL 2015
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (161.831 KB) | DOI: 10.33476/jky.v23i1.91

Abstract

Paparan merkuri secara kontinyu dalam saluran pencernaan, dapat menyebabkan kresistensi bakteri terhadap merkuri. Bakteri resisten merkuri bermanfaat pada proses detoksifikasi merkuri anorganik dengan mereduksinya menjadi logam merkuri yang tidak toksik. Penelitian ini bertujuan untuk menganalisis gen 16SrRNA dan menguji daya reduksinya terhadap merkuri HgCl2 dari bakteri resisten merkuri anorganik Isolat F2.1 dan F2.2 yang diisolasi dari feses pasien dengan tambalan amalgam gigi di Puskesmas Bahu Manado.  Analisis Gen 16SrRNA menggunakan metode Polymerase chain reaction (PCR) dan kadar merkuri dianalisis dengan menggunakan metode Cold-Vapor Atomic Absorption Spectrophotometry (CV-AAS). Hasil BLAST urutan nukleotida gen 16SrRNA menunjukkan bahwa kedua isolat bakteri tersebut mempunyai kemiripan 100% terhadap gen 16SrRNA bakteri Escherichia coli yang terdapat pada GenBank. Hasil analisis daya reduksi merkuri diperoleh bahwa dalam waktu 1, 12, dan 24 jam dapat menurunkan kadar merkuri dalam media berturut-turut untuk isolat F2.1: 82,2%, 87,1% dan 99,2% dan untuk isolat F2.2: 79,5%, 89,2% dan 99,3%.Hasil penelitian menunjukkan bahwa bakteri isolat F2.1 dan F2.2 yang diisolasi dari feses ialah bakteri Escherichia coli dan dapat mereduksikan HgCl2 hampir 100% dalam waktu 24 jam sehingga bakteri tersebut dapat digunakan pada penelitian selanjutnya untuk proses detoksifikasi merkuri organik.Continuous exposure to mercury in the digestive tract, can cause mercury-resistance bacteria. Mercury resistance bacteria are useful in detoxifying processes of inorganic mercury to the reduct form of non toxic metallic mercury.This study aims to analyze 16SrRNA gene and test for mercury reduction ability of inorganic mercury resistant bacteria isolates F2.1 and F2.2, isolated from feces of patients with tooth amalgam at Puskesmas Bahu in Manado. 16SrRNA gene analysis was done using polymerase chain reaction (PCR) and mercury levels were analyzed by using the method of Cold - Vapor Atomic Absorption Spectrophotometry (CV - AAS). BLAST results of nucleotide sequence of 16SrRNA gene showed that both the bacterial isolates had 100% similarity to the 16SrRNA gene of Escherichia coli bacteria found in GenBank. The results of the analysis showed that the  reduction ability of mercury in 1 , 12 , and 24 hours can reduce levels of mercury in a row for a media F2.1 isolates: 82.2%, 87.1% and 99.2% and for isolates F2.2: 79.5%, 89.2% and 99.3%. The results showed that the bacterial isolates F2.1 and F2.2 isolated from fecal is Escherichia coli bacteria and may reduce the HgCl2 almost 100% within 24 hours so that the bacteria can be used in future studies to inorganic mercury detoxification process.
Co-Authors Aaltje E. Manampiring Aaltje E. Manampiring Aaltje E. Manampiring Aaltje Ellen Manampiring Aaltje Manampiring Aaltje Manampiring Aaltje Manampiring Aaltje Manampiring Abdul Hawil Abas Abubakar, Poetry M.S. Abubakar, Poetry Melinda Adeanne Wullur Adithya Yudistira Adrian Umboh Alamri, Firdaus Alexander S. L. Bolang Andrew Pangemanan, Andrew Angelique C. M. Tanan Anggelia Nelisa Kapantow Anita Linda Yohana Pratasik Anita Mamuaya Anjely Jessica Makatempuge Annisa Hamka Antasionasti, Irma Apolonia Amelintje Gusmão Awoah Astrid Noviera Iksan Axl L. Windah Ayu Fauzia Malinda Baharuddin, Fatmawaty Bahter, Julia V.F. BEIVY JONATHAN KOLONDAM Bernadus, Zefanya Bernadus, Zefanya G Billy J Kepel Billy J Kepel Billy J. Kepel Billy J. Kepel Billy Johnson Kepe Billy Kepel Billy Kepel Billy Kepel Budiarso, Fona D. Budiarso, Fona D. H. Butue, Leobernard Catur Riany Cheisy Anastasya Gratia Lengkong Chintya G. Derek Damaris Damaris Datu, Olvie S. Datu, Olvie Syenni Debbie Retnoningrum Debora Tandi Defny S. Wewengkang, Defny S. Deliyana Lanti Desye Nurmalita Tanan Devianitta Sarapi Dian Pratiwi Dina V. Rombot Dinda V. Lomotu Donald Andreas Chandra Dos D. L. E. Br Siagian Eka Mulya Muthalib Elly Juliana Suoth Elly Juliana Suoth Elly Suoth Endang Triastuti Engka, Joice Nancy Eva M. Mantjoro Fatmawaty Badaruddin Feti Fatimah Feybe Ireine Kumayas Fona Budiarso Fona Budiarso Fona Budiarso Fona D.H. Budiarso Frans S. M. Mintardjo Frederika Filly Toad Frenly Wehantouw Frisky S. Badi, Frisky S. Friyan Criscanus Manopo Gabriela Clementine Ranti Gandu, Inggrid V. Gani, Maria A Gani, Maria A. Gayatri Citraningtyas Gerung, Widya Hana Putri Gerungan, Yizreel Y. Grenshannya Anasthasya Pua Greta J. P. Wahongan Gueen L. G. Naray Gustaaf A. E. 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