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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Agrohorti Bulletin (e-Journal) Jurnal Hortikultura Indonesia Jurnal Ilmiah Aplikasi Isotop Radiasi Jurnal Hortikultura AGRIVITA, Journal of Agricultural Science Jurnal AGROSAINS dan TEKNOLOGI Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biologi Tropis Journal of Tropical Crop Science AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Molekul: Jurnal Ilmiah Kimia Menara Perkebunan Akta Agrosia Agrotech Journal
Syarifah Iis Aisyah
Bogor Agricultural University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Environmental Science Immunology & microbiology Materials Science & Nanotechnology

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Journal : AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian

 1 
Analisis komparatif kandungan metabolit pada daun mutan tanaman Torbangun (Plectranthus amboinicus (Lour.) Spreng.) Aisyah, Syarifah Iis; Rusmiyati, Henny; Sukma, Dewi; Damanik, Rizal; Nurcholis, Waras
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 4 No 1 (2020): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v4i1.109

Abstract

Torbangun (Plectranthus amboinicus) merupakan tanaman obat dari keluarga Lamiaceae. Pada masyarakat Batak, daun torbangun digunakan untuk meningkatkan produksi air susu. Keragaman kandungan fitokimia dapat ditingkatkan salah satunya dengan teknik iradiasi sinar gamma untuk program pemuliaan tanaman. Pada penelitian ini, metode metabolomik yang tidak tertarget digunakan untuk mengevaluasi profil senyawa pada bagian daun tanaman mutan dari iradiasi sinar gamma dan kontrol. Kandungan senyawa planlet mutan dan kontrol dianalisis dengan GC-MS. Data GC-MS dianalisis dengan kemometrik dengan menggunakan hierarchical cluster analysis (HCA). Keragaman kandungan kimia ditunjukkan oleh planlet mutan dan kontrol daun torbangun. Hasil analisa planlet kontrol torbangun menunjukkan terdapat lima senyawa utama diantaranya hydroxymethylfurfurole (35,15%), brevifolin (7,69%), 3-Dihydro-3,5-Dihydroxy-6-Methyl-4H-Pyran-4-one (DDMP) (13,13%), stigmasterol (6,51%) dan ferruginol (8.63%). Hasil analisa mutan torbangun memiliki kandungan senyawa DDMP (19,57%), neophytadiene (8,47%), linolenic acid (9,10%), ferruginol (7,61%), stigmasterol (14,14%) dan gamma.-sitosterol (7,08%). HCA menunjukkan tiga komponen senyawa yang berbeda antara planlet kontrol dan mutan yaitu kenaikan kandungan senyawa DDMP dan stigmasterol, serta penurunan kandungan hydroxymethylfurfurole pada mutan torbangun.
Analisis komparatif kandungan metabolit pada daun mutan tanaman Torbangun (Plectranthus amboinicus (Lour.) Spreng.): Analisis komparatif kandungan metabolit pada daun mutan tanaman Torbangun (Plectranthus amboinicus (Lour.) Spreng.) Syarifah Iis Aisyah; Henny Rusmiyati; Dewi Sukma; Rizal Damanik; Waras Nurcholis
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 4 No 1 (2020): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v4i1.109

Abstract

Torbangun (Plectranthus amboinicus) is a medicinal plant belonging to the Lamiaceae family. In Batak society, the torbangun leaves are consumed to increase breast milk production. The phytochemical variability can be improved by gamma-ray irradiation for plant breeding programs. In this work, a non-targeted metabolomics method was followed to evaluate the compounds profile of irradiated mutant and wild-type on leaves of P. amboinicus. GC-MS analyses evaluated the mutant putative (derived from gamma-ray irradiation) and wild-type plantlets. The resulting of GC-MS data were analyzed with chemometric analysis using hierarchical cluster analysis (HCA). The chemical diversity was showed in mutant and wild-type P. amboinicus. The major metabolite in plantlet wild-type were hydroxymethylfurfural (35.15%), brevifolin (7.69%), 3-Dihydro-3,5-Dihydroxy-6-Methyl-4H-Pyran-4-one (DDMP) (13.13%), stigmasterol (6.51%) and ferruginol (8.63%). However, the compound of DDMP (19.57%), neophytadiene (8.47%), linolenic acid (9.10%), ferruginol (7.61%), stigmasterol (14.14%) and gamma-sitosterol (7.08%) were identified in mutant plantlet. HCA showed diversity between wild-type and mutant plantlets, which highest content of DDMP and stigmasterol and lowest of hydroxymethylfurfural of mutant plantlet.
Performance of Putative Mutants and Genetic Parameters of Plectranthus amboinicus (L.) through Mutation Induction With Colchicine Kunto Wibisono; Syarifah Iis Aisyah; Waras Nurcholis; Sri Suhesti
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 5 No 2 (2021): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1378.134 KB) | DOI: 10.33019/agrosainstek.v5i2.247

Abstract

Genetic enhancement in vegetatively propagated crops can be done through mutation induction. Colchicine-induced mutation is one of the methods that can be employed to increase plant genetic diversity. This study aimed to determine the effect of colchicine on the performance and genetic parameters of MV3 generation of Plectranthus amboinicus (L.). This study was conducted at the Laboratory of Agricultural Seed Management, Plantation Research and Development Center, from June 2018 to June 2019. Nodes and shoots were used as explants. Mutation induction was performed using colchicine at concentrations of 0%, 0.02%, 0.04%, and 0.06%. Explant regeneration and subculture were done on MS0 medium. The number of plantlets yielded were 59 (0% concentration of colchicine), 60 (0.02%), 81 (0.04%), and 80 plantlets (0.06%), respectively. Results indicated that colchicine-induced mutation in an in vitro culture was able to generate high genetic diversity in both quantitative and qualitative characters of the plantlets. At the concentration of 0.04%, colchicine produced the highest frequency of putative mutants (28.4%). Genetic parameters in MV3 generation of P. amboinicus plantlets showed that five quantitative characters, i.e. plantlet height, number of leaves, number of shoots, leaf length, and number of roots had high heritability values at a concentration around the LC50 value (0.0275%).
Co-Authors , Krisantini Abdul Hakim Agus Purwito Arum Sholikhah ASEP SAEFUDDIN Awang Maharijaya Aziz, Sandra A Azri Kusuma Dewi Azzahra, Elmi Irmayanti Bagas Akmala Putra Budi Marwoto Dewi Sukma Dia Novita Sari Diny Dinarti Endang Gati Lestari Eny Rolenti Togatorop HAJRIAL ASWIDINNOOR Hasna Dama Hayati, Dhieni Helfi Eka Saputra Helfi Eka Saputra Helfi Eka Saputra Joko Ridho Witono Kartiman, Roni Kartiman, Roni Khotimah Khotimah Kunto Wibisono Kunto Wibisono Kunto Wibisono Kurniati, Ridho Latifah K Darusman M. Rizal M. Damanik M. Rizal M. Damanik M. Rizal Martua Damanik Marthin, Yodi Marwoto, Budi Masna Maya SINTA Monica Christina Natalia Muhamad Syukur Muhammad Ridha Alfarabi Istiqlal Muhammad Syukur Mulyana, Erik Natalia, Monica Christina Nella Angelina Simanjuntak Ni Made Armini Wiendi Nurul Komala Qisthi Kustia Rahman RAGAPADMI PURNAMANINGSIH Rahman, Qisthi Kustia Rahmi Yunianti Ritonga, Arya Widura Rizal Damanik Rizal Damanik Rohim Firdaus Roni Kartiman Rosa Yunita Rusmiyati, Henny S Sudarsono Sarsidi Sastrosumarjo Setiawan, Farensa Ikman Dedi Siti Hartati Yusida Saragih Sobir Sobir Sobir Sobir Sobir Sobir Sobir Soeranto Human Sri Suhesti Sri Suhesti Sri Suhesti Sri Wahyuni Sukma, Dewi Sulassih Supijatno, . Suryawati, nFN Trikoesoemaningtyas Trikoesoemaningtyas Utin Winarni Waras Nurcholis Waras Nurcholis Wijaya Murti Indriatama Wijaya Murti Indriatama Wulan Septiningtyas Kurniajati yahidah Rosyidah Anshori Yasmin, Zahra Fadhlia Yukarie Ayu Wulandari Yukarie Ayu Wulandari Yustia Yulianti Zahra Fadhlia Yasmin