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Insecticidal activity of the extracts of Piper retrofractum fruit and Tephrosia vogelii leaf and their mixtures against Crocidolomia pavonana Djoko Prijono; R. Dewi Ratna Wulan; Ferdi Ferdi; Nur Alfi Saryanah
CROPSAVER Vol 3, No 2 (2020)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v3i2.31305

Abstract

This laboratory work was carried out to evaluate the insecticidal activity of the extracts of Piper retrofractum (Piperaceae) fruit and Tephrosia vogelii (Fabaceae) leaf and their mixtures against the cabbage head caterpillar, Crocidolomia pavonana. Ground plant materials of the two plant species were extracted separately with n-hexane and methanol. The results of leaf-residue feeding bioassays showed that P. retrofractum (Pr) and T. vogelii (Tv) hexane extracts had strong insecticidal activity against C. pavonana larvae (LC95 < 0.5%) and were more active than their respective methanol extracts. Pr and Tv hexane extract had a moderate and a rather weak contact effect on C. pavonana larvae, respectively. In feeding tests, Pr + Tv (1:1) hexane and methanol extract mixtures indicated synergistic joint effect both at LC50 and LC95 level, whereas in the contact test, the mixture of Pr + Tv (1:1) hexane extract was synergistic at the LC50 level but antagonistic at the LC95 level. In choice tests, antifeedant effects of Pr and Tv hexane extracts at LC25 to LC70 levels on C. pavonana larvae followed a concentration-dependent fashion. Thus, separate or mixed P. retrofractum and T. vogelii extracts are potential alternatives for the control of C. pavonana.
Sinergisme Ekstrak Piper aduncum dan Tephrosia vogelii terhadap Penggerek Batang Padi Kuning, Scirpophaga incertulas Muhammad Sigit Susanto; Djoko Prijono
Agrikultura Vol 26, No 1 (2015): April, 2015
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.77 KB) | DOI: 10.24198/agrikultura.v26i1.8454

Abstract

AbstractSynergism of Piper aduncum and Tephrosia vogelii Extracts against the Rice Yellow Stem Borer, ScirpophagaincertulasRice (Oryza sativa) is one of the important staple food crops in the world. One of the important pests on riceis the rice yellow stem borer (RYSB) Scirpophaga incertulas . One of the alternative control techniques thatcan be applied is by using botanical insecticides. The purpose of this study was to determine the synergismbetween Piper aduncum fruit and Tephrosia vogelii leaf extracts against RYSB larvae. P. aduncum and T.vogelii extracts, either tested separately or in mixtures with concentration ratios of 1:1, 2:1, and 1:2, wereeffective against RYSB larvae. The treatments with these extracts caused death in YRSB larvae at 72 hoursafter treatment in which the affected larvae showed internal tissue damage symptom. The most toxic testmaterial to RYSB larvae was P. aduncum and T. vogelii extract mixture with a concentration ratio of 1:2.LC50 and LC95 of P. aduncum extract were 0.175% and 0.126%, respectively, while those of T. vogelii extractwere 1.620% and 2.075%, respectively. P. aduncum and T. vogelii extract mixture with 1:1 ratio had LC500.056% and LC95 0.143%, while that with 2:1 ratio had LC50 0.025% and LC95 0.149%. LC50 and LC95 of themixture with 1:2 ratio were 0.016% and 0.083%, respectively. P. aduncum and T. vogelii extract mixture atthe three concentration ratios were more effective than P. aduncum and T. vogelii extracts appliedseparately and synergistic to RYSB larvae.Keywords: Botanical Insecticide, Piper aduncum, Scirpophaga incertulas , Synergism, Tephrosia vogelii .AbstrakPadi (Oryza sativa) merupakan salah satu komoditas penting pangan dunia. Salah satu hama penting padatanaman padi adalah penggerek batang padi kuning, Scirpophaga incertulas . Salah satu cara pengendalianyang dapat diterapkan adalah dengan menggunakan insektisida nabati. Tujuan penelitian ini adalahmenentukan sinergisme ekstrak buah Piper aduncum dan daun Tephrosia vogelii terhadap larva S.incertulas . Perlakuan ekstrak P. aduncum dan T. vogelii baik secara terpisah maupun campuran pada nisbahkonsentrasi 1:1, 2:1, dan 1:2 efektif terhadap larva S. incertulas . Perlakuan ini dapat menimbulkan kematianpada S. incertulas dengan gejala kerusakan jaringan larva instar 1 pada 72 jam setelah perlakuan. Bahan ujiyang paling beracun terhadap larva S. incertulas adalah campuran ekstrak P. aduncum dan T. vogelii dengannisbah konsentrasi 1:2. LC50 dan LC95 ekstrak P. aduncum masing-masing 0,175% dan 0,126%. Sementara ituLC50 dan LC95 ekstrak T. vogelii masing-masing 1,620% dan 2,075%. Campuran ekstrak P. aduncum dan T.vogelii pada nisbah konsentrasi 1:1 memiliki LC50 0,056% dan LC95 0,143%. Sementara itu campuran ekstraktersebut pada nisbah konsentrasi 2:1 memiliki LC50 0,025% dan LC95 0,149%. LC50 dan LC95 campuranekstrak tersebut pada nisbah konsentrasi 1:2 masing-masing 0,016% dan 0,083%. Campuran ekstrak P.aduncum dan T. vogelii pada ketiga nisbah konsentrasi lebih efektif dibandingkan dengan ekstraktunggalnya dan bersifat sinergis terhadap larva S. incertulas .Kata kunci: Insektisida Nabati, Piper aduncum, Tephrosia vogelii , Scirpophaga incertulas , Sinergisme.
AKTIVITAS INSEKTISIDA EKSTRAK BUAH CABAI JAWA (Piper retrofractum) TERHADAP Helopeltis antonii (HEMIPTERA: MIRIDAE) GUSTI INDRIATI; DADANG DADANG; DJOKO PRIJONO
Jurnal Penelitian Tanaman Industri Vol 21, No 1 (2015): Maret 2015
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v21n1.2015.33-40

Abstract

ABSTRAKHelopeltis antonii merupakan salah satu hama pada tanaman kakao,teh, dan jambu mete yang menyerang pucuk dan buah dengan menusukkanstiletnya untuk mengisap cairan sehingga menyebabkan kerusakan.Penelitian bertujuan menguji toksisitas ekstrak buah Piper retrofractum(cabai jawa) terhadap imago, pengaruh konsentrasi subletal terhadap nimfaketurunan, persistensi terhadap mortalitas dan oviposisi H. antonii.Penelitian dilaksanakan di Laboratorium Fisiologi dan ToksikologiSerangga, Institut Pertanian Bogor dan Laboratorium Proteksi Tanaman,Balai Penelitian Tanaman Industri dan Penyegar Sukabumi, mulai Mei2013 sampai April 2014. Buah mentimun digunakan sebagai inangpengganti untuk perbanyakan serangga uji H. antonii di laboratorium danmedia pengujian. Hasil penelitian menunjukkan kematian imago H.antonii, akibat perlakuan ekstrak P. retrofractum 0,05-0,3%, sudah terjadipada 24 jam setelah perlakuan (JSP). Sementara itu, pada 24 dan 48 JSPterjadi penurunan tingkat kematian H. antonii. Setelah 48 JSP, hanyaterjadi sedikit peningkatan kematian H. antonii. LC 50 dan LC 95 ekstrak P.retrofractum pada 120 JSP masing-masing 0,20 dan 0,49%. Jumlah nimfaketurunan yang dihasilkan pada perlakuan subletal ekstrak P. retrofractum0,203% (LC 50 ) lebih sedikit dibandingkan dengan konsentrasi 0,141%(LC 25 ). Ekstrak P. retrofractum yang dipajankan di bawah sinar mataharihingga 5 hari masih efektif terhadap imago (mortalitas 80%), tetapi tidakefektif dalam menghambat peletakan telur H. antonii. Penghambatanpeletakan telur terhadap imago H. antonii pada perlakuan ekstrak P.retrofractum 0,98% (2 × LC 95 ) yang dipajankan di bawah sinar matahariselama 0 dan 1 hari, dengan indeks penghambatan oviposisi 22,7 dan23,8%. Keefektifan ekstrak P. retrofractum perlu diuji di lapangan untukmenilai kelayakan dalam pengendalian H. antonii.Kata kunci: insektisida botani, mortalitas, oviposisi, persistensiABSTRACTHelopeltis antonii is cocoa, tea, and cashew nuts important pest thatcauses damage by sucking plant sap from shoots and nuts. This study wasconducted to test toxicity of Piper retrofractum fruit extract on adults,sublethal effect on the production nymphal progeny, and persistenceagainst mortality and oviposition of H. antonii. This study was conductedat The Fisiology and Insect Toxicology Laboratory, Plant ProtectionDepartement, Bogor Agricultural University and The Plant ProtectionLaboratory of Indonesian Industrial and Beverage Crops Research Institutefrom Mey 2013 to April 2014. Cucumber was used as a host substitute forrearing the test insect and as the testing medium. The results show thatadult mortality, due to the P. retrofractum leaf extract treatment 0.05-0.3%, has occured at 24 hours after treatment (HAT). Meanwhile, H.antonii mortality has decreased on 24 and 48 HAT. After 48 HAT, only aslight increased in H. antonii mortality. LC 50 and LC 95 of P. retrofractumextract at 120 HAT were 0.20 and 0.49%, respectively. The treatment atsublethal concentrations (LC 25 and LC 50 ) markedly decreased nymphalprogeny number. P. retrofractum extract suspensions at LC 95 and 2 × LC 95exposed under sunlight for 5 days were still effective against H. antoniiadults (80% mortality), but were not effective in females inhibitingoviposition. The oviposition inhibiting activity was observed only in thetreatment with P. retrofractum extract at 2 × LC 95  exposed under sunlightfor 0 and 1 day in which the oviposition deterrency indices were 22.7 and23.8% respectively. Key words: botanical insecticides, mortality, oviposition, persistence
PENGARUH PEMANASAN DAN PENYIMPANAN TERHADAP AKTIVITAS INSEKTISIDA EKSTRAK LERAK (Sapindus rarak) PADA LARVA Crocidolomia pavonana (F.) (LEPIDOPTERA: CRAMBIDAE) Gracia Mediana; Djoko Prijono
Agrovigor Vol 7, No 2 (2014): September
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.222 KB) | DOI: 10.21107/agrovigor.v7i2.1443

Abstract

Hama Crocidolomia pavonana merupakan salah satu kendala biotik penting pada budi daya tanaman sayuran famili Brassicaceae. Salah satu sarana pengendalian hama yang ramah lingkungan ialah insektisida nabati. Penelitian ini bertujuan menentukan pengaruh pemanasan dan penyimpanan terhadap aktivitas insektisida ekstrak buah lerak (Sapindus rarak) pada larva C. pavonana. Irisan buah lerak digiling dalam akuades menggunakan blender. Tiga macam ekstrak lerak yang diuji ialah (1) ekstrak tanpa pemanasan dan langsung digunakan, (2) ekstrak yang disiapkan dengan pemanasan pada suhu 40 °C dan langsung digunakan, serta (3) ekstrak dengan pemanasan dan disimpan pada suhu  ruang selama 7 hari. Setiap ekstrak diuji pada enam taraf konsentrasi dengan menggunakan metode perlakuan daun pakan. Larva instar II C. pavonana diberi pakan daun kubis perlakuan selama 2 hari dan daun tanpa perlakuan pada 2 hari berikutnya. Data kematian larva uji dicatat setiap hari sampai hari ke-4 dan diolah dengan analisis probit. Secara umum mortalitas larva C. pavonana pada semua perlakuan meningkat tajam antara 24 dan 48 jam setelah perlakuan (JSP). Berdasarkan LC95 pada 96 JSP, ekstrak lerak dengan pemanasan (LC95 2,53%) sekitar 1,7 kali lebih toksik terhadap larva C. pavonana daripada ekstrak lerak tanpa pemanasan (LC95 4,19%), tetapi toksisitas ekstrak tersebut menurun sekitar 2,9 kali lipat setelah disimpan pada suhu ruang selama 7 hari (LC95 7.43%). Selain mengakibatkan kematian, perlakuan dengan ekstrak lerak juga dapat menghambat perkembangan larva C. pavonana dari instar II ke instar IV.Kata kunci: insektisida nabati, ketahanan simpan, cara penyiapan, toksisitas.
Insecticidal Activity of Extracts of Aglaia spp. (Meliaceae) against Cabbage Cluster Caterpillar Crocidolomia binotalis Zeller (Lepidoptera: Pyralidae) Djoko Prijono; Partomuan Simanjuntak; Bambang W. Nugroho; Sudarmo Sudarmo; Shinta Puspitasari
Jurnal Perlindungan Tanaman Indonesia Vol 7, No 2 (2001)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.10069

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Insecticidal potential of eleven species of Aglaia (Meliaceae) was evaluated in the laboratory against the cabbage cluster caterpillar, Crocidolomia binotalis. The feeding treatment of second-instar larvae C. binotalis for 48 hours with ethanol twig extract of A. odorata at 0.5% caused 98.7% larval mortality; leaf and twig extracts of A. elaeagnoidea caused 17.3% and 6.7% mortality, respectively; twig extracts of A. argentea, A. formosana, and A. latifolia caused only 1.3% mortality each; whereas extracts of the other six Aglaia species were inactive (0% mortality). Further tests with A. odorata showed that twigs gave the most active extract compared to other plant parts (leaves, flowers, and roots), and air-drying of plant materials for 2 weeks markedly decreased the activity of the derived extracts. The active extracts also delayed the development of surviving larvae in similar degree to the level of their lethal effect. LC50 of ethyl acetate fraction of A. odorata twig extract and its main active compound, rocaglamide, against C. binotalis larvae were 310.2 and 31.4 ppm, respectively. This active compound was about 8.7 times less potent than azadirachtin (LC50 3.6 ppm).
Aktivitas Insektisida Ekstrak Kulit Batang Empat Famili Tumbuhan terhadap Ulat Krop Kubis Crocidolomia pavonana (F.) Edy Syahputra; Djoko Prijono; Dadang Dadang; Syafrida Manuwoto; Latifah K Darusman
Jurnal Perlindungan Tanaman Indonesia Vol 10, No 1 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12208

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The objective of this study was to evaluate the insecticidal activity of bark extracts of 35 plant species belonging to four families (Clusiaceae, Lecythidaceae Meliaceae, and Sapindaceae) against the cabbage head caterpillar Crocidolomia pavonana. Extraction of the test plant materials were performed with maceration method using ethanol and methanol continued by counter-current distribution separation in ethylacetate and water. Bark extract preparation of each plant species was tested against C. pavonana larvae by leaf-feeding method at concentration of 0.25% and 0.5%. Second-instar C. pavonana larvae were fed extract-treated broccoli leaves for 48 hours, then were presented with untreated leaves until the surviving larvae reached the fourth-instar larvae. The number of dead larvae was recorded. The phytochemical test of bark extract of Calophyllum soulattri was done by qualitative method. The results showed that the bark extracts of C. soulattri (Clusiaceae), Barringtonia sarcostachys (Lecythidaceae), and Aglaia tomentosa (Meliaceae) exhibited good insecticidal activity. Methanol extract of C. soulattri barks possessed strong insecticidal activity against C. pavonana larvae with LC95 of 0.21%. This extract showed positive response in alkaloid and triterpenoid qualitative tests. Further studies are needed to identify insecticidal compounds in those active extracts.
KERENTANAN WERENG BATANG COKELAT (NILAPARVATA LUGENS) DARI ENAM LOKASI DI PULAU JAWA TERHADAP TIGA JENIS INSEKTISIDA Erwin Cuk Surahmat; Dadang .; Djoko Prijono
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 16 No. 1 (2016): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.956 KB) | DOI: 10.23960/j.hptt.11671-81

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Susceptibility of the rice brown planthopper (Nilaparvata lugens) from six locations in Java to three insecticides. The rice brown planthopper (BPH), Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), is one of the important rice pests in Indonesia. Despite relatively frequent use of insecticides to control this pest, data on BPH resistance to insecticides commonly used by farmers in Indonesia is scarce. This study was conducted to assess the susceptibility of BPH from six locations in Java to three insecticides, i.e. BPMC, imidacloprid, and pymetrozine, and to find out the historical use of insecticides by rice farmers. The first laboratory generations of BPH adults were subjected to insecticide treatments using a leaf dip residual method as described in IRAC Method No 005. Resistance factor (RF) for BPMC and imidacloprid was determined by comparing LC50 of each insecticide against BPH field populations with that against the reference population, whereas the susceptibility of BPH to pymetrozine was assessed based on inhibition of BPH population development. The three insecticides used in this study were commonly used by farmers in the six sampling locations and those insecticides were mostly used twice per rice growing season. BPH populations from all six locations as well as the reference population were susceptible to pymetrozine in which the treatment with pymetrozine at 50 ppm (one-fifth of the recommended field rate) caused 100% inhibition of BPH population development. BPH from five locations showed resistance to imidacloprid (RF > 4), but that from Pasuruan did not (RF 2.0). BPH from Indramayu was resistant to BPMC (RF 6.6) whereas those from the other five locations were not (RF < 4). Exposure of the reference population to a sublethal dose of insecticides (LC50 or IC50) decreased the susceptibility of the third BPH generation to pymetrozine and BPMC but not to imidacloprid.
KEEFEKTIFAN TIGA JENIS INSEKTISIDA NABATI TERHADAP KUTU PUTIH PEPAYA PARACOCCUS MARGINATUS DAN KEAMANANNYA TERHADAP LARVA KUMBANG PREDATOR CURINUS COERULEUS Ahmad Sifa; Djoko Prijono; Aunu Rauf
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 13 No. 2 (2013): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (115.702 KB) | DOI: 10.23960/j.hptt.213124-132

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Effectiveness of three botanical insecticides against the papaya mealybug Paracoccus marginatus and their safety to the predatory Curinus coeruleus larvae. Preparations of Tephrosia vogelii (Tv) leaf extract (0.5% and 1% w/v), Annona squamosa (As) seed extract (0.5% and 1%), and Cinnamomum multiforum (Cm) leaf essential oil (1% and 2%), and their mixtures (Mix-1: Tv 0.25% + As 0.25% + Cm 0.5%; Mix-2: Tv 0.5% + As 0.5% + Cm 1%) were tested for their effectiveness on third-instar nymphs of Paracoccus marginatus by spraying the test materials on undetached papaya leaves and by direct spraying on the test insects using Potter spray tower. Tv extract was also applied on the test insects placed on undetached papaya leaves. The safety test was done by direct spraying of the test materials on the predatory Curinus coeruleus larvae using Potter spray tower. Tv and As extracts at a concentration of 1% each are potential to be used for the control of P. marginatus. Spraying of T. vogelii extract on the test P. marginatus nymphs placed on papaya leaves was more effective than spraying of the test materials on papaya leaves or direct spraying on the test insects only. The treatment with Cm essential oil required twice the concentration of Tv and As extracts to obtain the same level of effectiveness. The treatment with Mix-2 caused lower P. marginatus mortality than the sum of mortality caused by its components applied separately. Nonetheless, the three botanical insecticides and their mixtures were safe to C. coeruleus larvae. On the other hand, although the synthetic insecticide imidacloprid (neonicotinoid), included in the study for comparison, was effective against P. marginatus, it was also toxic to the predatory C. coeruleus larvae so its use should be avoided or limited.
SEDIAAN INSEKTISIDA CALOPHYLLUM SOULATTRI: AKTIVITAS INSEKTISIDA DAN RESIDU TERHADAP LARVA CROCIDOLOMIA PAVONANA DAN KEAMANAN PADA TANAMAN Edy Syahputra, Djoko Prijono, .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 7 No. 1 (2007): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (122.506 KB) | DOI: 10.23960/j.hptt.17%p

Abstract

Insecticide Preparation of Calophyllum soulattri: Insecticidal and Residual Activity Against Crocidolomia pavonana and Its Savety on Crops. Bioassays were done using leaf-residual method with Crocidolomia pavonana larvae as test insect. The number of dead larvae was recorded daily and was analyzed using probit method. The results showed that methanol extract, and dichloromethane fraction of C. soulattri bark possessed strong lethal effect against C. pavonana larvae with LC50 of 0.15%, and 0.05%, respectively. Dichloromethane fraction of 21 EC and 21 WP sprayed in suspension consentration of 1% on potted broccoli plant had residual activity around 16%-26,7% with range of half-lives of 5.9-7.7 days. Dichloromethane fraction at concentration of 0.2% and 0.3% formulated as EC did not cause phytotoxic to broccoli, tomato, chilli, soybean, red bean, onion, cucumber, and maize. Concerning with this potency, further studies are needed to identify insecticidal compounds.
PENGARUH FRAKSI AKTIF AGLAIA HARMSIANA PERKINS (MELIACEAE) TERHADAP FISIOLOGI LARVA SPODOPTERA LITURA (F.) (LEPIDOPTERA: NOCTUIDAE) Eka Candra Lina, Djoko Prijono, dan Dadang .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 6 No. 1 (2006): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (187.864 KB) | DOI: 10.23960/j.hptt.161-8

Abstract

The effect of active fraction Aglalia harmsiana Perkins (Meliaceae) to physiology of Spodoptera litura (F.) (Lepidoptera : Noctuidae) larvae. This study was conducted to evaluate the physiological interferences in the soybean armyworm Spodoptera litura caused by active fractions of Aglaia harmsiana seed extract. The activity of the test materials was assessed by dietary preparation and topical application method. Repeated fractionations of A. harmsiana extract by chromatographic methods yielded two active fractions, designated as fraction 2-7 (0.0184%) and 2-8 (0.3773%). The results showed that fraction 2-8 had strong insecticidal activity against S. litura larvae, with LC50 by topical application and dietary preparation were 0.49% and 0.0044%, respectively. A sublethal treatment with the active fraction (LC25) reduced the relative growth rate, efficiency of conversion of ingested food, and efficiency of conversion of digested food by 52.9%, 42.9%, 49.6% with topical application and 66.7%, 50%, 63.8% with dietary preparation method. The treatments with that fraction at LC10 and LC25 to the fourth instars reduced the activity of invertase enzyme by 34% and 47%, but increased the activity of trehalase by 6.2% and 12.5% as compared with controls.