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Analisa Kerusakan Jaringan Akar Lamun Thalassia hemprichii yang Terpapar Logam Berat Kadmium (Cd) Wahyu Noviarini; Dini Ermavitalini
Jurnal Sains dan Seni ITS Vol 4, No 2 (2015)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM), ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (404.3 KB) | DOI: 10.12962/j23373520.v4i2.13545

Abstract

Salah satu logam berat berbahaya yang berpotensi mencemari laut adalah kadmium. Logam berat kadmium merupakan unsur logam yang berbahaya di permukaan bumi. Thalassia hemprichi merupakan lamun yang dapat tumbuh dengan tingkat toleransi yang tinggi. Penelitian ini bertujuan untuk menguji pengaruh logam berat kadmium (Cd) terhadap kerusakan jaringan akar T. hemprichii. Penelitian ini dilakukan secara in-vitro selama 30 hari dengan 4 perlakuan perbedaan konsentrasi Cd yaitu 0 ppm, 0,01 ppm, 0,05 ppm dan 0,1 ppm. Metode yang digunakan untuk pengamatan jaringan akar yaitu metode parafin menggunakan hand-microtomy. Hasil dari penelitian ini yaitu T. hemprichii yang dikulturkan secara in-vitro dengan paparan kadmium sebesar 0,1 ppm dapat menyebabkan kerusakan sel akar berupa degradasi pada jaringan pengangkut.
Pengaruh Cekaman Nitrogen dan Fotoperiode terhadap Kurva pertumbuhan Kultur Nannochloropsis sp. Sumarni Dwirejeki; Dini Ermavitalini
Jurnal Sains dan Seni ITS Vol 8, No 1 (2019)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM), ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j23373520.v8i1.41642

Abstract

Konsumsi energi meningkat dari tahun ke tahun dan 95% terpenuhi dari bahan fosil yang jumlahnya terbatas. Biodiesel sebagai alternatif sumber energi dari tanaman jarak (Jatropha curcas) dan kelapa sawit memiliki kemampuan oksidasi yang rendah pada suhu dingin, produksi lipid membutuhkan waktu lama serta kandungan lipid yang berkisar 35% dan 75,6%. Di sisi lain, Nannochloropsis sp. dapat memproduksi lipid mencapai 90% dari berat kering biomassa pada kondisi tercekam, panen lebih cepat, mudah dibudidayakan. Waktu panen kultur Nannochloropsis sp. dapat direkayasa berdasarkan nutrisi, suhu, aerasi, salinitas, fotoperiode. Penelitian ini bertujuan untuk menguji pengaruh cekaman nitrogen dan fotoperiode terhadap lama waktu panen kultur. Nannochloropsis sp., dikultur pada kondisi normal untuk mendapatkan masa starter (setengah eksponensial kultur). Kultur Nannochloropsis sp. pada masa starter diberikan perlakuan kombinasi nitrogen pada pupuk Conway terdiri dari 0%, 25%, 50%, 75% dan 100% NaNO3 dengan fotoperiode (terang:gelap) 12:12, 16:8 dan 24:0. Kultur Nannochloropsis sp. dengan nitrogen terbatas memiliki fase eksponensial yang lebih singkat sedangkan pada perlakuan fotoperiode dengan terang lebih lama, kultur memiliki nilai OD yang paling tinggi. Waktu panen paling cepat yaitu perlakuan dengan nitrogen 0%, nilai OD paling tinggi pada pemberian nitrogen 100% pada berbagai fotoperiode.
Pengaruh Ethyl Methane Sulphonate (EMS) terhadap Morfologi Akar Kecambah Padi (Oryza sativa) Varietas Lallodo Retty Asti Ogtavia Pratiwi; Dini Ermavitalini
Jurnal Sains dan Seni ITS Vol 8, No 1 (2019)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM), ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (294.249 KB) | DOI: 10.12962/j23373520.v8i1.42153

Abstract

Padi (Oryza sativa L.) merupakan tanaman pangan pokok lebih dari separuh penduduk dunia. Padi varietas Lallodo merupakan padi lokal dataran tinggi Toraja dengan nilai produksi gabah giling tertinggi. Salah satu upaya mendapatkan varietas unggul demi memenuhi kebutuhan pangan adalah induksi mutagen kimia, salah satunya Ethyl Methane Sulphonate (EMS). Tujuan dari penelitian ini adalah untuk mempelajari efek EMS pada morfologi akar. Parameter yang diamati pada penelitian ini adalah daya kecambah, panjang akar dan bentuk rambut akar kecambah padi Lallodo yang dipapar dengan larutan EMS pada konsentrasi 0%; 0.25%; 0.5%; 0.75%; 1%; 1.25%; 1.5%, dan 2%. Selanjutnya nilai daya kecambah dan panjang akar dianalisis dengan menggunakan ANOVA oneway dengan dilanjutkan uji Duncan.  Hasil penelitian menunjukkan bahwa perlakuan EMS berpengaruh terhadap panjang akar kecambah padi dimana perlakuan EMS 0%; 0,25%; dan 0,50% mempengaruhi panjang akar secara tidak signifikan. Perlakuan EMS juga berpengaruh pada daya kecambah pada hari 1 hingga 3, tetapi tidak berpengaruh pada daya kecambah hari 4. Pengamatan visual morfologi rambut akar dengan mikroskop dan SEM (Scanning Electron Microscope) menunjukkan bahwa semakin tinggi konsentrasi EMS maka rambut akar semakin pendek dan kerapatannya semakin jarang.
Astaxanthin-Producing Microalgae Identification Using 18S rRNA : Isolates from Bangkalan Mangrove Waters and Sowan Tuban Northern Waters, East Java, Indonesia Dini Ermavitalini; Siska Yulia Rukhmana; Thalita Meidina; Leonardo Pascalis Dimas Cahyo Baskoro; Triono Bagus Saputro; Ni’matuzahroh Ni’matuzahroh; Hery Purnobasuki
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 3 (2021): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.64882

Abstract

Microalgae are a group of micro-sized photosynthetic organisms that range from prokaryotic cyanobacteria to eukaryotic algae. Microalgae are widely used as a source  of natural food, cosmetic ingredients, food ingredients, and a source of pigments. This study aims to identify species of four microalgae isolates named B1, B2, B3, and S2 from Bangkalan Mangrove Waters and Sowan Tuban Northern Waters, and to determine their astaxanthin pigment concentration under 1 M NaCl. Species identification was carried out through a molecular approach by utilization of an 18S rRNA gene marker. A quantitative test of astaxanthin concentration was carried out by spectrophotometric analysis. Molecular identification results show that isolates B1 and B3 are closely related to Chlorella sp., while isolates B2 and S2  are closely related to Picochlorum maculatum. Moreover, under salinity stress condition of 1 M NaCl shown a significant decrement of astaxanthin production compared to the control treatment. At 1 M NaCl, the astaxanthin content of isolate B1 was 4x10-5 mgL-1, isolate B2 was 2x10-5 mgL-1, isolate B3 was 1x10-5 mgL-1, and isolate S2 was 6x10-6 mgL-1. All in all, isolate S2 has the highest astaxanthin among the other isolates at normal conditions, while under salt stress regime, isolate B1 shown to be the best source for astaxanthin. 
The Effect of Nitrogen Stress And Photoperiode on Growth and Fatty Acid Content of Nannochloropsis sp. Dini Ermavitalini; Sumarni Dwirejeki; Sri Nurhatika; Triono Bagus Saputro
Akta Kimia Indonesia Vol 4, No 1 (2019)
Publisher : LPPM, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (793.657 KB) | DOI: 10.12962/j25493736.v4i1.5089

Abstract

Energy consumption increases from year to year and 95% is fulfilled by fossil materials which are limited, so that a replacement energy source is needed. Biodiesel is a fuel from renewable sources such as plants or animals. Biodiesel from Jatropha curcas and palm oil has been produced but oxidation is low in cold temperatures and long time to production. On the other hand, Nannochloropsis sp., as marine microalgae can produce lipids as biodiesel material reaches 90% of the biomass dry weight in stress condition, It’s higher than lipid that produced by Jatropha curcas (35% DW) and palm (75,6% DW). Nannochloropsis sp. also short growth, easily propagated. Production of lipids and TAGs of  Nannochloropsis sp. can be engineered based on nutrient, temperature, aeration, salinity, photoperiod. This study aims to examine the effect of nitrogen stress and photoperiode on biomass, chlorophyll content, fatty acid profile, content of TAG. Nannochloropsis sp. cultured from the starter then cultured in variation of nitrogen stress and phoroperiode.  In the last exponential, culture analized in quantitative to get the biomass and chlorophyll content. Fatty acid and TAG analized by qualitative. The interaction between nitrogen and photoperiode did not affect the biomass of  Nannochloropsis sp. but it affects the chlorophyll content where the chlorophyll content of P14 (N100, 16: 8) is 7.367 mg/L. It is not significantly different from the treatment P6 (N25, 24: 0) of 3.339 mg/L and treatment P15 (N100, 24: 0) 5.665 mg/L. All cultures with a treatment combination showed triacylglycerol content. The results of the treatment of fatty acid profiles P14 (N100, 16: 8) contain more varied fatty acids compared to P1 (N0, 12:12), P2 (N0, 16: 8), P8 (N50, 62: 8), P10 (N75 , 12:12) which consists of lauric acid, hexagonal acidrionate, palmitic acid, linoleic acid, oleic acid, stearate acid, eicosanoic acid.The result showed that the treatment of nitrogen stress and photoperiode affecting the growth and fatty acid content of Nannochloropsis sp.
Pengembangan Kebun Bibit Herbal Organik Sebagai Unit Pendukung Konsep Agrowidyawisata di Desa Oro-Oro Ombo, Kota Batu, Malang Nurul Jadid; Kristanti Indah Purwani; Tutik Nurhidayati; Ardy Maulidy Navastara; Dini Ermavitalini; Wirdhatul Muslihatin; Marsudi
JURPIKAT (Jurnal Pengabdian Kepada Masyarakat) Vol 1 No 2 (2020)
Publisher : Politeknik Dharma Patria

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37339/jurpikat.v1i2.324

Abstract

Pergeseran pola hidup di dalam masyarakat yang saat ini cenderung back to nature menyebabkan semakin meningkatnya permintaan obat herbal organik. Namun demikian, budidaya tanaman obat secara organik dan pengelolaannya secara professional masih jarang dilakukan. Kota Batu, Malang Jawa Timur terkenal sebagai agropolitan. Pengabdian masyarakat ini bertujuan untuk mengembangkan kebun bibit herbal organik yang berfungsi sebagai area pembibitan tanaman herbal dan agrowidyawisata di Desa Oro-Oro Ombo Kota Batu, Malang. Pengabdian masyarakat dilakukan bersama mitra Kelompok Tani Hutan (KTH) Panderman. Adapun metode yang dilakukan berupa penggalian informasi lokal dan pendampingan pengembangan kebun bibit herbal. Hasil pengabdian menunjukkan bahwa Desa Oro-Oro Ombo, Kota Batu Malang merupakan kawasan potensial untuk budidaya tanaman herbal. Diharapkan dengan adanya kegiatan ini, partisipasi masyarakat desa terhadap pengembangan budidaya herbal organik semakin meningkat. Sehingga hal ini dapat berdampak positif pada peningkatan ekonomi masyarakat desa.
PELATIHAN KOMPOSTING SAMPAH SKALA RUMAH TANGGA DALAM PENGELOLAAN SAMPAH BERBASIS MASYARAKAT DESA KETEGAN TANGGULANGIN SIDOARJO Dini Ermavitalini; Nurul Jadid; Wirdhatul Muslihatin; Triono Bagus Saputro; Maya Shovitri; Endry Nugroho Prasetyo; Noor Nailis Saadah; Kristanti Indah Purwani
Jurnal ABDI: Media Pengabdian Kepada Masyarakat Vol. 5 No. 1 (2019)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/ja.v5n1.p39-43

Abstract

Along with the increasing number of people and all economic activities carried out, waste becomes acontamination that continues to leave problems. Sidoarjo Regency has 18 sub-districts with 350 villagesand a population of around 2.3 million people with a high level of economic growth. According to the 2017Sidoarjo Regency Environmental and Hygiene Office (DLHK), reported that Sidoarjo district residentsdispose of household waste around 0.5 kg per day. DLHK identifies the lack of Integrated WasteManagement Sites (TPST) and Final Waste Disposal Sites (TPAS) to accommodate and manage wastefrom the Sidoarjo Regency community. The lack of TPST and TPAS is not a problem in wastemanagement if active community involvement is involved in processing household waste known asCommunity Based Waste Management (PSBM). This abdimas method is a campaign about theimportance of the role of the community in improving environmental status and composting training withraw materials in the form of kitchen waste with a simple household-scale tool located in the KeteganVillage office, Tanggulangin Sub-district, Sidoarjo. The participants were very enthusiastic about takingpart in the training and were eager to practice household composting on a household scale for the need tofertilize plants planted in the yard. Participants want monitoring by the service team on the compostingresults that have been carried out by each participant.
Greenhouse Potential based on Ecotourism and Education for Sustainable Village Economic Resilience Lilik Nurindah Sari; Tutik Nurhidayati; Maya Shovitri; Enny Zulaika; Nengah Dwianita Kuswytasari; Arif Luqman; Nur Hidayatul Alami; Kristanti Indah Purwani; Nurul Jadid; Dini Ermavitalini; Imam Wahyudi Farid; Ciptian Weried Priananda
IPTEK Journal of Proceedings Series No 1 (2023): The 1st International Conference on Community Services and Public Policy (ICCSP) 2022
Publisher : Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j23546026.y2023i1.16378

Abstract

Indonesia has many rural areas with diversity and uniqueness in each region that have developed into eco-tourism. The village that is being developed as an eco-tourism destination is expected to improve the welfare of the surrounding community. Developed eco-tourism can provide jobs for residents in the village. Eco-tourism is also one of the developments to preserve ecosystems in rural areas. This eco- tourism sector can support community welfare and sustainable rural development. One approach to the development of rural areas is through village ecotourism. Village Ecotourism is a rural area with several special characteristics that can be used as a tourist destination. One way to improve the development of village ecotourism is by adding new facilities that lead to educational tourism, i.e., greenhouse facilities. The existence of this greenhouse can be used as a means of science education about a more advanced agricultural system with a controlled environmental system. Greenhouse technology can improve the quality and quantity of plant productivity, thereby increasing people's income, and producing healthier organic plant products. Greenhouses can also be used as educational tourism facilities for various science education activities and simple agricultural training. Training for residents can also be carried out, for community empowerment, such as training in planting, fertilizing, nurseries, processing plant products, and the process of packaging plant products. This review summarizes the various potentials of Greenhouse development for the development of education-based village ecotourism and provides references for further research that focuses on community service, which is increasing sustainable village economic resilience.
Penanaman Karakter Peduli Lingkungan Melalui Program Edukasi Konservasi Keanekaragaman Hayati Sejak Usia Dini Nova Maulidina Ashuri; Noor Nailis Sa’adah; Edwin Setiawan; Dini Ermavitalini; Triono Bagus Saputro; Awik Puji Dyah Nurhayati
Sewagati Vol 5 No 3 (2021)
Publisher : Pusat Publikasi ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1021.146 KB) | DOI: 10.12962/j26139960.v5i3.29

Abstract

Program Pendidikan Konservasi Keanekaragaman Hayati sejak Usia Dini bertujuan untuk membangun karakter peduli lingkungan, menambah pengetahuan, minat, kepedulian dan kepedulian dalam melestarikan, melindungi dan memanfaatkan keanekaragaman hayati di sekitar lingkungannya yang dimulai sejak usia dini. Program pendidikan dilaksanakan dengan penyuluhan, simulasi iptek, dan advokasi (pendampingan). Program tersebut telah dilaksanakan di Taman Kanak-kanak Siti Masithoh dan di tempat pendidikan Al Qur’an yaitu Al Abror melalui pembuatan "Kebun Kehati" serta pelaksanaan seminar parenting dengan tema "Membangun Karakter Peduli Anak. Lingkungan Melalui Program Pelestarian Keanekaragaman Hayati Sejak Dini di Desa Boro, Kedungwaru, Tulungagung, Jawa Timur "yang dihadiri oleh siswa dan orang tua / wali siswa, perwakilan guru dari Taman Kanak-kanak dan Sekolah Dasar di desa serta para pengawas dan Kepala Sekolah Dinas Pendidikan Pendidikan pelestarian keanekaragaman hayati ini juga dapat menambah pengetahuan dan inovasi generasi penerus untuk dapat memanfaatkan, mengelola, melindungi dan melestarikan sumber daya alam sekitar.Kegiatan ini juga dilakukan dalam rangka meningkatkan kesadaran setiap individu terhadap kondisi lingkungannya.Selain itu, keberhasilan program ini juga membutuhkan kendali dari masyarakat dan partisipasi negara.
Efect of Gamma 60Co Irradiation on The Growth, Lipid Content and Fatty Acid Composition of Botryococcus sp. Microalgae Dini Ermavitalini; Niki Yuliansari; Endry Nugroho Prasetyo; Triono Bagus Saputro
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.6783

Abstract

Botryococcus sp. is one of microalgae species that has a high lipid content as much as 75% of their dry weight. But, lipid production by microalgae is regulated by their environmental condition (pH, light, temperature, nutrition, etc). Mutagenesis induced by Gamma 60Co irradiation can be utilized to alter the Botryococcus sp. genetic to get microalgae mutant strain that can produce a higher lipid content than the wild strain. Botryococcus sp. was irradiated with different doses of gamma ray of 60Co  (0, 2, 4, 6, and 10 Gy),  and the effect  on the growth, lipid content, and fatty acid composition of microalgae were observed. Research design used is random complete (RAL) with 95 %  confident level for quantitive analysis based on the biomass and lipid contents. More over fatty acid composition was analyzed by Gas Cromatography-Mass Spectrometry (GC-MS). Results showed that Gamma irradiated gave an effect on growth and lipid content of Botryococcus sp. But between the control treatment (0 Gy) with microalgae irradiated dose of 2 Gy, 4 Gy and 6 Gy were not significantly different. Whereas between the control with 10 Gy irradiated was significantly different. The highest biomassa and lipid content are found in 10 Gy irradiated microalgae with 0.833 gram biomass and 41% lipid content. Fatty acid profile of Botryococcus sp. control has 6 fatty acids while 10 Gy irradiated microalgae has 12 fatty acids, with the long-chain fatty acids increased, whereas short-chain fatty acids decreased.