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In Vitro Selection of Local Maize (Zea mays) on NaCl Stress and Its Genetic Characterization using RAPD Triono Bagus Saputro; Fathin Finariyah; Siti Dianawati; Nur Fadlilatus Sholihah; Dini Ermavitalini
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6934

Abstract

Maize (Zea mays) is one of graminae plants that widely spread for many purposes wheter in food industry, feed, or bioenergy. Those high utilization required an increment in production, but unfortunately the needs were not meet to the demands since conversion of agricultural area increase lately. Indonesia has 99.093 km of shoreline that recognize as marginal land where the salinity is high as well. This research try to obtain tolerant variant from two local cultivars that planted in Madura Island. Manding and Talango varieties were used as an explant for callus induction stage in MS supplemented with 2.4 D. The result show that 4 ppm of 2.4 D were the best concentration to induce the callus in both varieties. The induced callus were exposed to medium MS that contain NaCl (0, 2500, 5000, and 7500 ppm). In 7500 ppm of NaCl, Manding variety has 100% of surviving callus, while Talango variety only 66.7%. Furthermore, Manding variety shows a better performance in callus weight improvement with 170 mg, while Talango shows no improvement of callus weight. The result of RAPD analysis indicate that the genome characteristic were different between initial callus and surviving callus. Out of ten primers, only five primers shows polymorphism i.e OPA 13, OPB 07, OPC 02, OPK 20, and OPU 19. Manding can be further developed as high tolerance variety in Salinity stress, thus it proposed to be developed furthermore.How to CiteSaputro, T. B., Finariyah, F., Dianawati, S., Sholihah, N. F., Ermavitalini, D. (2016). In Vitro Selection of Local Maize (Zea mays) on NaCl Stress and Its Genetic Characterization using RAPD. Biosaintifika: Journal of Biology Biology Education, 8(3), 344-352. 
Pemanfaatan Greenhouse sebagai Wahana Edukasi di Desa Kebontunggul Kecamatan Gondang Kabupaten Mojokerto Tutik Nurhidayati; Maya Shovitri; Nengah Dwianita Kuswytasari; Enny Zulaika; Nur Hidayatul Alami; Arif Luqman; Dini Ermavitalini; Kristanti Indah Purwani; Wirdatul Muslihatin; Imam Wahyudi Farid; Ciptian Weried Priananda; Sri Fatmawati
Sewagati Vol 7 No 6 (2023)
Publisher : Pusat Publikasi ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j26139960.v7i6.515

Abstract

Salah satu desa yang memiliki potensi untuk dikembangkan sebagai desa wisata adalah desa Kebontunggul Kecamatan Gondang Kabupaten Mojokerto. Desa tersebut memiliki banyak potensi desa wisata yang strategis. Disisi lain ada keinginan untuk melakukan kegiatan pertanian berbasis teknologi dalam skala greenhouse untuk mendukung usaha wisata di Desa Kebontunggul. Adanya potensi dan keingginan untuk maju tersebut maka langkah pertama yang dilakukan dalam pengabdian masyarakat adalah pembuatan greenhouse sebagai sarana untuk pengembangan teknologi IoT. Selain itu adanya greenhouse di desa menjadi salah satu pengembangan desa dengan ikon terbaru berupa wisata edukasi. Hasil survei kebermanfaatan greenhouse menunjukkan bahwa greenhouse bermanfaat bagi masyarakat Kebontunggul untuk wahana wisata yang menarik banyak wisatawan.