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All Journal Jurnal Kedokteran Brawijaya Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Jurnal Kesehatan Reproduksi Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY Universa Medicina Jurnal Gizi Klinik Indonesia Majalah Farmaseutik Jurnal Kesehatan Reproduksi Molekul: Jurnal Ilmiah Kimia Journal of Community Empowerment for Health JKKI : Jurnal Kedokteran dan Kesehatan Indonesia

Mae Sri Hartati Wahyuningsih

- Department Of Pharmacology And Therapy, Faculty Of Medicine, Public Health And Nursing, Universitas Gadjah Mada - Herbal Medical Center, Faculty Of Medicine, Public Health And Nursing, Universitas Gadjah Mada

Author-ID : 287503

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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EFEK KOMBINASI EKSTRAK Tithonia diversifolia (HEMSLEY) A.GRAY DAN Curcuma domestica VAL TERHADAP NODUL DAN BERAT BADAN TIKUS MODEL KANKER Halimah, Wahyu Nur; Pratiwi, Woro Rukmi; Wahyuningsih, Mae Sri Hartati
Majalah Farmaseutik Vol 13, No 1 (2017)
Publisher : Fakultas Farmasi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar

Kembang bulan [Tithonia diversifolia (Hemsley) A. Gray] merupakan kandidat tanaman berpotensi antikanker yang telah dibuktikan sebelumnya memiliki efek sitotoksik terhadap sel kanker WiDr, IC50=61,55 ug/mL. Kunyit (Curcuma domestica Val.) banyak digunakan dalam pengobatan tradisional, mengandung kurkuminoid yang memiliki aktivitas antikanker dengan memodulasi fungsi imun yang dimediasi oleh limfosit. Dengan adanya efek imunostimulan dari kunyit apabila dicampur dengan kembang bulan sebagai kandidat antikanker diharapkan akan bersinergis lebih baik. Tujuan penelitian ini untuk mengkaji potensi kombinasi ekstrak etanol T. diversifolia dan C. domestica pada hewan model kanker dengan cara menghitung berat badan, jumlah nodul dan volume nodul hewan coba. Penelitian ini adalah penelitian kuasi eksperimental. Kombinasi ekstrak etanol 70% daun kembang bulan dan rimpang kunyit dibuat suspensi dan dibagi menjadi 3 dosis yaitu dosis 40:150mg, 80:150mg, dan 160:150mg. Kemudian diberikan peroral pada masing-masing kelompok tikus yang telah mengalami kanker payudara setelah diinduksi dengan DMBA. Kemudian diamati berat badan, jumlah nodul, dan volume nodul tikus yang dibandingkan dengan kelompok kontrol negatif dan kelompok kontrol positif (doksorubisin). Persentase perubahan pada masing-masing kelompok perlakuan dibandingkan dan diuji secara statistik menggunakan analisis Anova. Hasil menunjukkan bahwa perbandingan berat badan tikus antara kelompok kontrol doksorubisin dan kelompok dosis 40:150mg, 80:150mg, dan 160:150mg menunjukkan tidak berbeda signifikan. Perbandingan volume nodul kanker payudara antara kelompok kontrol dan kelompok uji menunjukkan hasil yang tidak berbeda signifikan. Kombinasi ekstrak etanolÂ kembang bulan dan rimpang kunyit tidak mempunyai efek signifikan terhadap berat badan dan nodul kanker payudara

SITOTOXICITY OF COMPOUND ISOLATED FROM THE LEAVES OF TITHONIA DIVERSIFOLIA (HEMSLEY) A.GRAY) AGAINST T47D, MCF-7 AND EVSA-T CELLS Arfian Bela Mahardika; Subagus Wahyuono; Mae Sri Hartanti Wahyuningsih
Majalah Farmaseutik Vol 12, No 2 (2016)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Tithonia diversifolia (Hemsley) A. Gray) is one of the plants that are traditionally used to treat various diseases. The previous studies using Bioassay Guided Isolation methods has obtained the active compound (MTT, HeLa cells; IC50 9.776 g / mL). The cytotoxic effects of active compounds against breast cancer cells is unknown. This study aims to determine the cytotoxicity of theactive compound isolated from the leaves of T. diversifolia on breast cancer cells (T47D; MCF-7 and EVSA-T). The isolated compounds from T. diversifolia tested their cytotoxicity against breast cancer cells (T47D, MCF-7 and EVSA-T), using MTT method with a series of concentration of 0.39 to 50 ug/mL. Each concentration of the group performed triplicate and calculated the percentage of cell growth inhibition with probit analysis to calculate the IC50 value. The test results showed that the active compound have a cytotoxic effect on T47D, MCF-7 and EVSA-T cells with IC50 values are respectively (2.44; 4.697; 3.522) ug/mL, so this compound potential to be developed as an anticancer agent, especially breastcancer

Effect of Tithonia Diversifolia (Hemsley) A.Gray and Curcuma Domestica Val Extracts Combination Against Nodule and Body Weight In Cancer Rats Model Mae Sri Hartati Wahyuningsih; Wahyu Nur Halimah; Woro Rukmi Prtiwi
Majalah Farmaseutik Vol 13, No 1 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Kembang bulan [Tithonia diversifolia (Hemsley) A. Gray] is a potentially anticancer plants that have been previously shown have cytotoxics effect on WiDr colon cancer cells, IC50=61.55µg/mL. Turmeric (Curcuma domestica Val.) is widely used in traditional medicine. Curcuminoids compounds from turmeric have potential anticancer activity with possibly modulate immune function mediated by lymphocytes. With the immunostimulant effect of turmeric when mixed with kembang bulan as an anticancer candidate is expected to synergize better. This study aims to determine the potential of T. diversifolia and C. domestica ethanolic extracts combination in cancer rats model by measuring weight, number of nodules, and nodule volume animals. The study was a quasi-experimental study. Mixed 70% ethanol extract of kembang bulan’s leaves and turmeric’s rhizome divided into 3 doses, 40:150mg, 80:150mg, and 160:150mg. Then it given to each group of rats by orally that had experienced breast cancer . After that, see potential on body weight, number of nodules, and nodule volume. Then compared with normal group, negative control group, and positive control group. Furthermore, the percentage change in each treatment group were compared and statistically tested using ANOVA analysis. Comparison of the results of rat’s body weight between the control group (doxorubicin) and test group showed significantly no different results. Comparison of the results of rat’s breast cancer nodule volume between the control group and the test group showed significantly no different results. A combination of 70% ethanol extract of kembang bulan’s leaves and turmeric’s rhizome have no significant effect on body weight and breast cancer nodules.

Comparison of Methanol, Ethyl Acetates and Chloroform Extracts of Zingiber zerumbet (L) Smith in Larvisidal Activities on Aedes aegypti (Diptera: Culicidee) Larvae Tri Murini; Mae Sri Hartati Wahyuningsih; Tri Baskoro T.Satoto
Majalah Farmaseutik Vol 13, No 2 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

The resistance of mosquito larvae to conventional larvicides has been increased in the recent years. Therefore, it is necessary to develop new larvacides from natural materials. Previous studies showed that Zingiber zerumbet (L.) Smith has a potential ability as a larvicide in Artemia salina Leach and Anopheles nunestovary. Nevertheless, the use of different solvents to observe the best larvicidal activity has not been known. This study aims to assess the larvicidal activity of methanol extract, ethyl acetate, and chloroform in Aedes aegypti larvae. This is an exploratory laboratory study with the posttest-only control group. The extraction solvents used were methanol, ethyl acetate, and chloroform. Twenty-five instar larvae (III-IV) of Ae.aegypti were treated with seven different concentrations of the three Z. rhizome extracts. The mortality rate of the larvae was calculated and analyzed using probit analysis regression to obtain LC50 and LC90 values. The LC50 of the methanol, ethyl acetate, and chloroform extracts are 153.57, 185.80, 3542.03 ppm; while the LC90 are 226.59, 274.70, and 5567.24 ppm, respectively. The study suggested that the methanol extract of Z. zerumbet rhizome has the most robust larvicidal activity against third-fourth instar larvae of Ae. aegypti among other extracts.

Effect of Extract Combination Tithonia diversifolia (Hemsley) A.Gray and Curcuma domestica Val. In Function of Heart and Kidney Rats of Cancer Model Angga Anugerah; Woro Rukmi Pratiwi; Mae Sri Hartati Wahyuningsih
Majalah Farmaseutik Vol 14, No 1 (2018)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Both Tithonia diversifolia (Hemsley) A. Gray leaves and (Curcuma domestica Val.) rhizome have bioactive compound which are an anticancer drug candidate. However, in vivo study about the effect of those to liver function and kidney function has never been carried out. The aim to determine the effect of ethanol extract T. diversifolia (Td) and C.domestica (Cd) combination to Sprague Dawley female rats liver and kidney function which have been induced with DMBA. This research used a quasi experimental study design. There were 31 female rats divided into 6 groups; healthy group; doxorubicin group; breast cancer model; extract combination I (n=5), extract combination II (n=5), extract combination III (n=5). DMBA used to initiate breast cancer was given twice a week for 5 weeks. After palpable breast cancer was showed up, the combination extract groups were given ethanol extract combination of kembang bulan leave and turmeric rhizome twice a day for 4 weeks orally with dosage ratio 40:150 mg/kgBW, 80:150 mg/kgBW, 160:150 mg/kgBW, respectively. Doxorubicin group was given 0,032mg/20gBW intraperitoneally once a day for 4 weeks. Liver and kidney function test were conducted on day 0 and the last day with SGOT, SGPT, Ureum and Creatinine level as the parameters measured. Comparison of liver and kidney function test results between extract combination groups and control groups showed no significant difference. The parameters measured were SGOT (p=0,847), SGPT (p=0,237), Creatinine (p=0,671), Ureum (p=459). Extract combination groups compared to control groups have no significant effect to liver and kidney function.

Optimasi Carbomer, Propilen Glikol, dan Trietanolamin Dalam Formulasi Sediaan Gel Ekstrak Etanol Daun Kembang Bulan (Tithonia diversifolia) Amira Fawwaz Tsabitah; Abdul Karim Zulkarnain; Mae Sri Hartanti Wahyuningsih; Dwi Aris Agung Nugrahaningsih
Majalah Farmaseutik Vol 16, No 2 (2020)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/farmaseutik.v16i2.45666

Ekstrak etanol daun kembang bulan atau Tithonia diversifolia telah diteliti memiliki kemampuan sebagai anti keloid yang baik. Tujuan penelitian ini adalah untuk mendapatkan formula gel dengan sifat fisik dan stabilitas yang baik. Metode yang digunakan dalam optimasi ini adalah Simplex Lattice Design. Formula optimum gel dibuat dari bahan carbomer, propilen glikol, dan trietanolamin. Parameter yang diukur adalah viskositas, pH, daya sebar, freeze and thaw cycles, dan sineresis. Hasil uji kemudian dianalisis menggunakan paired t-test.Hasil penelitian menunjukan bahwa formula optimum gel terdiri dari 0,81% carbomer, 5,9% propilen glikol, dan 0,58% trietanolamin dengan viskositas 155,96 d.Pas, daya sebar 4,06 cm, pH 5,50, dan sineresis 0,64%. Basis gel yang dihasilkan bening, memiliki viskositas 156,10 ± 2,87 d.Pas, daya sebar 3,95 ± 0,07 cm, pH 5,50 ± 0,00, dan sineresis 0,46 ± 0,00 %. Sediaan basis gel stabil dalam uji stabilitas fisik. Gel ekstrak etanol daun kembang bulan yang dihasilkan memiliki viskositas 77,70 ± 2,30 d.Pas, daya sebar 4,55 ± 0,21 cm, pH 5,32 ± 0,01, dan sineresis 1,42 ± 0,28 %. Sediaan gel ekstrak etanol daun kembang bulan kurang stabil dalam uji stabilitas fisik pada viskositas yang mengalami penurunan, daya sebar yang mengalami kenaikan, pH yang mengalami kenaikan, dan sineresis yang meningkat.

Penurunan Laktat Dehidrogenase Plasmodium falciparum strain D10 pada pemberian Fraksi Tithonia diversifolia (Hemsley) Rul Afiyah Syarif; Mae Sri Hartati Wahyuningsih; Mustofa Mustofa; Ngatidjan Ngatidjan
Majalah Farmaseutik Vol 17, No 1 (2021)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/farmaseutik.v17i1.57817

Penelitian terdahulu menunjukkan bahwa fraksi ke-6 (F6) merupakan fraksi aktif (F-akt) daun Tithonia diversifolia (Hemsley) A.Gray yang menghambat pertumbuhan Plasmodium. Pertumbuhan parasit ini memerlukan energi yang diperoleh dari aktivitas laktat dehidrogenase (LDH). Penelitian ini bertujuan mengkaji aktivitas antiplasmodium fraksi aktif T. diversifolia terhadap kadar LDH kultur P. falciparum. Kultur Plasmodium falciparum strain D10 stadium cincin dibagi menjadi kelompok eritrosit tidak terinfeksi (KTI), eritrosit terinfeksi (KI), dan eritrosit terinfeksi Plasmodium yang diberi F6 (F-akt) T. diversifolia (KI+F-akt) konsentrasi 9,38-150 µg/mL. Kultur diinkubasi 48 jam. Media kultur diukur kadar LDH-nya secara enzimatik. Adanya perbedaan LDH antar kelompok dianalisa dengan Anova. Penghambatan aktivitas LDH (IC50) ditetapkan dengan analisa probit. Kadar LDH kelompok KI (362,33 ± 133,18 U/L) lebih tinggi daripada KTI (270,33 ± 65,85 U/L) (p>0,05). Pemberian F-akt pada KI menyebabkan kadar LDH parasit lebih rendah daripada KI. Kadar LDH parasit yang diberi F-akt konsentrasi 9,38; 18,75; 37,50; dan 150 µg/mL secara berturut-turut adalah 365,5 ± 129,5; 210,5 ± 1,5; 195,5 ± 81,5; dan 111,5 ± 53,5 U/L. Tidak ada perbedaan kadar LDH antar kelompok penelitian (p>0,05). F-akt T. diversifolia mampu menghambat LDH P. falciparum strain D10 dengan nilai IC50 = 39,22 µg/mL.Kata kunci: Tithonia diversifolia, laktat dehidrogenase, Plasmodium falciparum

Co-Authors Abdul Karim Zulkarnain Achmad Fudholi Adiguno Suryo W Adiguno Suryo W, Adiguno Suryo Alam, Gemini Amira Fawwaz Tsabitah Angga Anugerah Annisa Nurul Pratiwi Arfian Bela Mahardika Argo D, Imono Arief Budiyanto Arif Yusuf Wicaksana Astuti, Puji Bayu Putra Bolhuis RLH Catharina Widiartini Damiana Sapta Candrasari Desyandri Desyandri Detty Siti Nurdiati, Detty Siti Dewi Aryanti Dita Ayu Dewi Laras Sati Dwi Aris Nugrahaningsih Dwiki Yuliya Rahmawati Edy Meiyanto Elly Wahyudin Elly Wahyudin Elsi Dwi Hapsari Elsi Dwi Hapsari, Elsi Dwi Elvira Santi Eti Nurwening Sholikhah Farida Fitriyanti Farida Fitriyanti Firandi Saputra Firandi Saputra, Firandi Firdaus, Insanul Fitranto Arjadi Fudholi, Achmad - Gatot S. Lawrence Gatot S. Lawrence Hajid Rahmadianto Hajid Rahmadianto Mardihusodo Hajid Rahmadianto Mardihusodo, Hajid Rahmadianto Halimah, Wahyu Nur Hardyanto Soebono Hayati, Farida I Dewa Putu Pramantara Ibnu G Gandjar, Ibnu G Ibnu G. Gandjar Ibnu G. Gandjar Ibnu G. Ganjar, Ibnu G. Indwiani Astuti Indwiani Astuti Ishandono Dachlan Ita Fauzia Hanoum K. Nooter KV Rao, KV M.Pd S.T. S.Pd. I Gde Wawan Sudatha . Mark T Hamann, Mark T Mark T. Hamann Mia Munawaroh Mirianti A. Manggau Mirianti A. Manggau, Mirianti A. Mufidah Mufidah Mufidah Mufidah Mustofa Mustofa Mustofa Mustofa Ngatidjan Ngatidjan Ngatidjan Ngatidjan Nia Krisniawati Nina Salamah Nina Salamah Nooter K Nugrahaningsih, Dwi Aris Agung Nungki Anggorowati Nur Arfian Nur Arfian, Nur Nur Falah Setyawati Nurfalah Setyawati Nyoman Nyoman Kertia Oostrum RG Pinus Jumariyatno Prasetyawati, Citra Prasetyo Tri Kuncoro Pratiwi, Woro Rukmi Probosuseno Probosuseno R.L.M. Bolhuis Rul Afiyah Syarif Sari, Dinar Sofia Mubarika Sofia Mubarika Sofia Mubarika Sofia Mubarika H Sri Kadarsih Soejono Sri Suharmi Sri Suharmi, Sri Subagus Wahyuono Subagus Wahyuono Subagus Wahyuono Subagus Wahyuono Sugiyanto . Sugiyanto Sugiyanto Sunarti Sunarti Susetyowati Susetyowati Susetyowati Susetyowati, Susetyowati Tatsuo Takeya, Tatsuo Teguh Aryandono Tri Baskoro T.Satoto Tri Baskoro Tunggul Satoto Tri Murini Tri Murini Tri Murini Tri Murini Wahyu Nur Halimah Wahyuono, Subagus Wahyuono, Subagus Wasis Pujiati Woro Rukmi Pratiwi Woro Rukmi Prtiwi Yohanes Widodo Wirohadidjojo Yulia Fauziyah Yuliani, Fara Silvia Yuniyanti, Mia Munawaroh

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