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Journal : BIOTROPIA - The Southeast Asian Journal of Tropical Biology

Bacteria from Arbuscular Mycorrhizal Fungi Spores Gigaspora sp. and Glomus sp. : Their Antagonistic Effects towards Soilborne Fungal Pathogens and Growth Stimulation of Gigaspora sp. in vitro Sri Wilarso Budi
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 20 No. 1 (2013)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2013.20.1.252

Abstract

Eight isolates bacteria were isolated from surface sterilized endomycorrhizal spores of Gigaspora sp and six isolates bacteria isolated from Glomus sp. The tests to  the antagonists against fungal pathogens and stimulation of endomycorrhizae hyphal development of Gigaspora sp  in vitro  showed that there were three.isolates (GG1, GG5 and GL3) that have the ability to inhibit the growth of pathogens and enhance the development of hyphae in vitro. Enzymatic activity test showed that there were seven isolates that produce cellulase and protease activity that is GG1, GG3, GG6, GG7, GL2, GL4 and GL6 Key words : Bacteria, Endomycorrhizal Spores, Antagonistic effects, Stimulation effects, Fungal pathogens
DNA BARCODE CHARACTERIZATION OF MISTLETOE INFESTATION IN TEAK CLONAL SEED ORCHARD (CSO) IN PADANGAN, EAST JAVA PROVINCE, INDONESIA Zainal Muttaqin; Sri Wilarso Budi; Basuki Wasis; Corryanti Corryanti; Iskandar Zulkarnaen Siregar
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 24 No. 2 (2017)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2755.603 KB) | DOI: 10.11598/btb.2017.24.2.651

Abstract

For effective teak plantation management, early detection system in controlling teak mistletoe requires various basic information, including degree of infestation and accuracy of the species names. Mistletoe infestations in teak and mistletoe species name have been reported, but there are still problems in identifying the correct species or subspecies due to morphological similarity.  The objective of this study was to clarify the species identity of hemi-parasitic mistletoe plants, which were found in teak Clonal Seed Orchard (CSO) in Padangan, East Java Province, Indonesia using DNA barcodes. Species identification of teak mistletoe based on leaf morphological characteristics and universal DNA barcode regions (i.e. matK and rbcL) were carried out. The results showed that the Canonical Discriminant Analysis (CDA) could differentiate Dendrophthoe pentandra and Macrosolen tetragonus based on leaf morphological characteristics. Variables having high correlation to distinguish both species were length of petiole,  width of the widest leaf, number of secondary leaf veins, leaf base shape, aspect ratio, form factor and perimeter ratio of diameter.  The results of  DNA barcoding showed that the two DNA barcode regions presented good  amplification and sequence results. Both DNA barcode regions successfully differentiated two species i.e. D. pentandra and M. tetragonus which belong to Loranthaceae family and have similar leaf morphological characteristics. Those regions were also able to identify Viscum articulatum and other species belonging to Santalaceae family. These results suggested that the two DNA regions could become recommended universal DNA barcode for identifying teak mistletoe.
JABON (Anthocephalus cadamba Roxb) POTENCY FOR REMEDIATING LEAD (Pb) TOXICITY UNDER NUTRIENT CULTURE CONDITION Luluk Setyaningsih; Yadi Setiadi; Sri Wilarso Budi; Hamim Hamim; Didy Sopandie
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 25 No. 1 (2018)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2018.25.1.712

Abstract

Information on metal adaptation of plants is necessary to understand the mechanism of heavy metal, including lead (Pb), remediation mediated by forest plants in contaminated land. This study aimed to find out the adaptation mechanism of jabon (Anthocephalus cadamba Roxb) seedlings to excessive lead level based on the tolerance index of growth performance and lead transport to plant tissue.  The seedlings were exposed to lead (Pb(NO3)2) with the concentrations of 0, 0.5, 1, 1.5, 5 and 10 mM in nutrient culture for 15 days. The result showed that the tolerance index (TI) of the seedlings was significantly decreased by Pb exposure up to 1.5 mM, but the TI values were more than 75%.  All seedlings died at Pb concentration of 5 mM and up.  Pb accumulated in all parts of the seedlings, with the highest concentration found in the leaves (735.9 ppm) under Pb concentration of 0.5 mM.   The Pb was found to be transported to the top portion of the seedlings indicated by Transport Factor (TF) that was more than 1. The results suggest that jabon can adapt to excessive Pb exposure up to 1.5 mM and has the potential as a remediator plant.
GENETIC VARIATION OF TEAK MISTLETOE (Dendrophthoe pentandra (L.) MIQ.) BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS Zainal Muttaqin; Sri Wilarso Budi; Basuki Wasis; Iskandar Zulkarnaen Siregar; Corryanti Corryanti
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 27 No. 2 (2020)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.519 KB) | DOI: 10.11598/btb.2020.27.2.1211

Abstract

Mistletoes are hemiparasitic macroparasite plants which interfere with trees and other wild plants in nutrient acquisition. As the plant has low leaf water potential, it draws water from teak wood tissues during the deciduous stage of the teak host, thereby killing the twigs and eventually, the teak tree. Mistletoes are also a key player in plant diversity. Therefore, the mistletoe population needs to be regulated not only as a parasite but also as a keystone species affecting biodiversity. Knowledge scarcity on the status of mistletoes includes its genetic variation. Hence, the purpose of this study is to analyze the level of genetic variation of teak mistletoe (Dendrophthoe pentandra) using RAPD marker. At Padangan teak Clonal Seed Orchard (CSO), it was randomly collected leaf samples from three layers of the mistletoe’s crown (upper, middle, and below) were taken from five host teak trees randomly selected from each of the sub-observation measure plots (OMP). Four OMP units inside the observation sample plots (OSP) (n = 3, 50 x 50 m) at different levels of infestation (light, moderate and heavy) were established. Analysis of the genetic variation and genetic distance of mistletoes hanging on the different crown layers were conducted using RAPD markers. The leaf samples from the crown layers, UU (upper crown and sub-section upper), UM (upper crown and sub-section middle), and UB (upper crown and sub-section below), which include U (upper crown) had significantly greater genetic variation (He = 0.181 – 0.255) than those from M layer (middle crown, He = 0.227) and the B layer (below crown, He = 0.114). Furthermore, the widest genetic distance significantly occurred between the mistletoes of the UB and B crown layers (0.310), whereas the nearest genetic distance significantly occurred between mistletoes of UU and UM layers in the upper crown (0.038). Practical implications of the low genetic variation in this study include the control of mistletoe D. pentandra infestation by means of restricting its population so that Perhutani State Owned Forestry Enterprise can maintain the level of damage below the economic threshold.
Co-Authors , Hariyadi AAboe B. Saidi Abdurrani Muin Adisti Permatasari Putri Hartoyo Agustina Puspita Dewi Aji Nuralam Dwisutono Akhmad Wahyudi Alfi Laila Zuhriansah Allaily Allaily Andi Sukendro Ani Kurniawati Arif Budi Setiawan Arum Sekar Wulandari Atang Sutandi Bambang S. Purwoko Baskoro Rochaddi Basuki Wasis Bedah Rupaedah Berry Oktavianto Betty Purwati Cahyo Wibowo Ceng Asmarahman Corryanti . Corryanti Corryanti Corryanti Corryanti Dadan Mulyana Dida Syamsuwida Didah Nur Faridah Didy Sopandie DWI ANDREAS SANTOSA Dwi Puspasari Arum Ramadhani Endah Retno Palupi Endang Suhendang Eny Widajati Erdy Santoso Erdy Santoso Faisal Al Asad Fatimah Nur Istiqomah Fiona Christina Fransisca Yosina Regina Luturmas Gunawan Gunawan Hamim Hamim Husna Husna Husna Husna Hutami Indah Pertiwi Ikbal . Ikbal Ikbal Imam Wahyudi Indrawan, Imam Wahyudi Intan Fajar Kemala Irdika Mansur Iskandar . Iskandar Iskandar Iskandar M. Lapanjang Iskandar Z Siregar ISKANDAR ZULKARNAEN SIREGAR Istomo . Iswandi Anas JAYANI, FARADILA MEI Jumadil Akhir Kartika Megawati Kartini Kramadibrata Kartini Kramadibrata Khoryfatul Munawaroh Lina Karlinasari Luluk Setyaningsih Maman Turjaman Maman Turjaman Maman Turjaman Maman Turjaman Maman Turjaman Maryani Fatimah Putri Hardhani Maya Melati MEITY SURADJI SINAGA MEITY SURADJI SINAGA Mohammad Agus Salim Mohammad Agus Salim Salim Muhammad Helmi Muttaqin, Zainal Nelly Fridayanti Noor F Mardatin NUNANG LAMAEK MAY Nurheni Wijayanto Panca Dewi MH Karti Prijanto Pamoengkas Putri Aurum Putri Aurum Rahmawati Rahmawati Sabti Indah Purwanti Safinah S. Hakim S Hakim Safinah Surya Hakim Satriyas Ilyas Siti Hanna Ghaida Sri Muryati Sri Muryati Sri Muryati Sudirman Yahya SUDIRMAN YAHYA Tedi Yunanto Teuku Tajuddin Tirsa Eka Saputri Ulfah Juniarti Siregar Wahono Sumaryono Yadi Setiadi Yadi Setiadi Yan Eka Prasetyawati YENNI BAKHTIAR YENNI BAKHTIAR YENNI BAKHTIAR Zainal Muttaqin