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Effect of Virgin Coconut Oil in The Treatment of Leucorrhea Caused by Candida Albicans Infection on Pregnant Women at Hospitals in Padang Putri Zelfitri Zen; Desmiwarti Desmiwarti; Sumaryati Syukur
Andalas Obstetrics And Gynecology Journal Vol 5, No 2 (2021)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/aoj.5.2.231-240.2021

Abstract

Introduction : According to WHO, the prevalence of reproductive health problems in women has reached 33% from all types of diseases. One of the most frequent reproductive health problems is fluor albus. The incidence of candidiasis in pregnant women has doubly increased, especially at third trimester, compared to women who were not pregnant. Candida should be considered a dangerous pathogen in early pregnancy.Objective : This study compared the number of colonies before and after the administration of virgin coconut oil (VCO) to address vaginal discharge caused by C. albicans infection in pregnant women.Material and methods : This is an experimental study on 36 pregnant women. Examination of fluor albus is done by applying vaginal swab before and after administration of 1 tablespoon of VCO orally three times a day dan 5cc of VCO was applied on the vagina and vulva for intervention group, meanwhile the control group was not given anything. Statistical analysis to test the significance is done by using T-test.Results : There was a difference on average number of colonies with higher number of colonies prior to administration (137.17 ± 16.35 vs 122.17 ± 14.42). The average number of colonies at the first examination was 96.06 ± 8.85 colonies and increased at the second examination to 112.89 ± 12.49 colonies.Conclusion : There was an effect of VCO administration on decreasing the number of colonies in the intervention group (p=0.000), while in control group, there was increasing average number of colonies in the second examination (p=0.000).Keywords: Candida albicans, fluor albus, pregnant women, virgin coconut oil
Differences of Reeda Scale in Wound Incision Abdomen Post Obstetrics / Gynaecology Laparotomy with Topical Virgin Coconut Oil (VCO) and Without Topical Virgin Coconut Oil (VCO) Nanda Tri Wahdini; Ferdinal Ferry; Sumaryati Syukur
Andalas Obstetrics And Gynecology Journal Vol 5, No 1 (2021)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/aoj.5.1.90-101.2021

Abstract

Introduction : Wounds are defects of normal anatomical structures and functions. Injuries can occur because of physical, chemical, or biological trauma. Wound healing is a complex process involving many cells, cytokines, growth factors, proteases, and extracellular matrix (ECM) that work together to restore the integrity of the injured tissue. Virgin Coconut Oil is an oil saturated with medium-chain fatty acids (MCFA), one of which is lauric acid. Based on our research, VCO has wound healing properties. Research conducted in vivo on rabbits and rats experienced an increase in collagen, fibroblast proliferation, and neovascularization which influenced accelerating wound healing.Objective : This study aims to determine the difference in the mean REEDA scale in abdominal incision wounds after obstetric / gynecology laparotomy with topical VCO and those without topical VCO.Material and methods : This study is an experimental research design with a randomized clinical trial that was conducted on 36 people divided into 2 groups (treatment and control). Post-obstetric / gynecology laparotomy patients were subjected to topical VCO application on the incision wound and the REEDA scale was calculated at 24, 48, and 72 hours post-surgery. Statistical analysis is using the Mann-Whitney test.Results : Age mean of the respondents in the treatment group was 32.28 ± 10.42 years old, while the untreated group was 30.5 ± 6.54 years old. The mean REEDA scale for abdominal incision wounds applied topical VCO at 24 hours was 3.17 ± 1.04, at 48 hours was 2.11 ± 1.08, and at 72 hours was 1.39 ± 0.85. The mean REEDA scale for abdominal incision wounds that were not treated with topical VCO at 24 hours was 3.83 ± 0.99, at 48 hours was 3.11 ± 1.37 and at 72 hours was 2.72 ± 1.41. The findings of the experimental analyses showed that there was no association with the use of topical VCO in surgical wounds on a 24-hour REEDA scale (p>0.05), there was a relationship between the use of topical VCO in surgical wounds on a 48-hour and 72-hour REEDA scale (p<0.05). In the care group, the mean REEDA scale decreased by 33.4 % from 24 to 48 hours and 34.1 % from 48 to 72 hours.Conclusion : There is a relationship with the use of topical VCO in post-abdominal incision wounds post obstetrics/gynaecology laparotomy with REEDA scale 48 hours and 72 hours post-surgery. The mean REEDA scale decreased by over 24 to 48 hours and 48 to 72 hours in the treatment group (who administered topical VCO in postoperative incision wounds).Keywords: VCO, REEDA scale, Wound healing
UJI PREKLINIS VIRGIN COCONUT OIL TERHADAP PENINGKATAN KADAR KOLESTEROL BAIK HDL, PENURUNAN KADAR TRIGLISERIDA, PROFIL KIMIA ASAM LEMAK (C6-C18) DAN OMEGA-3 PADA SERUM DARAH TIKUS PUTIH (Mus musculus) Sumaryati Syukur; Rina Dahlyanti; Titi Sumanti; Yulia Murni; Zoni Hidayat; Helmi Arifin; Endang Purwati; Risfaheri -
Jurnal Riset Kimia Vol. 1 No. 1 (2007): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v1i1.79

Abstract

 ABSTRACT The Virgin Coconut Oil (VCO) products have different qualities and controversy effects of lipids metabolism. This research has been used one of VCO product that was produced by fermentation method using Lactobacillus sp. It has high amount of lauric acid (C12) up to 51 %, caprilic acid (C8) 8.9 %, capric acid (C10) 7 % include Omega-3, 6 and 9, vitamins A, D, E, K and three kinds of phytohormone. The ratio of Omega-3 and 6 was very proportional, therefore it is important to investigate the Pre Clinic Test of animal experiment. Pre Clinic Test of dietary VCO as food supplement has been determined by using 40 mice’s, which divided into 4 groups. Feeding on egg yolk to Group I (negative control), Group II (positive control) increased cholesterol level. The others were Group III (egg yolk and VCO 2 %) and group IV (egg yolk and VCO 4 %). It was determined the total of cholesterol, HDL at 10th, 20th and 30th day treated by using the enzymatic methods. The dietary of VCO 2-4 % resulted in significant increases in HDL levels from 32 % to 69 %. The dietary of VCO 4 % for four weeks did not toxic to mice metabolism. Triglycerides level decreased 50 % from 177 to 85 and similar resulted to cholesterol ratio. Feeding on VCO for 4 weeks, the SCFA and MCFA not detected in serum of mice. The LCFA (C16) palmitate in significant decreased from 0.96 to 0.1%. The significant level of Omega-3 increased more than three times in serum of mice dietary VCO 2-4 %.  Keywords: Coconut Oil, Lactobacillus
DETERMINATION OF CALCIUM (Ca) AND MAGNESIUM (Mg) CONTENT IN CACAO (Theobroma cacao Linn) FERMENTATION AND NON FERMENTATION BY SPECTROPHOTOMETRY Wiranda -; Sumaryati Syukur; Hermansyah Aziz
Jurnal Riset Kimia Vol. 3 No. 1 (2009): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v3i1.104

Abstract

 ABSTRACT Cacao beans contain many kinds of mineral, magnesium (Mg), calcium (Ca), Zinc (Zn), Phosphor (P) and etc. This study investigated magnesium (Mg) and calcium (Ca) in fermentation and non fermentation cacao beans by atomic absorption spectrophotometry. Mg and Ca, content in non fermentation cacao beans of green and red variety are 453 µg/g, 466 µg/g, and 491 µg/g, 445 µg/g. Mg and Ca, contents  in fermentation cacao beans of green and red variety are, 596 µg/g, 528 µg/g, and 554 µg/g, 505 µg/g. Fermentation make magnesium (Mg) and calcium (Ca) content increase significantly. Keywords : Theobroma cacao Linn, fermentation, spectrophotometry.
POTENSI BAKTERI ASAM LAKTAT DALAM MENGHASILKAN BAKTERIOSIN SEBAGAI ANTIMIKROBA DAN PENGUKURAN BERAT MOLEKULNYA DENGAN SDS-PAGE DARI ISOLAT FERMENTASI KAKAO Urnemi -; Sumaryati Syukur; Endang Purwati; Sanusi Ibrahim; Jamsari -
Jurnal Riset Kimia Vol. 4 No. 2 (2011): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v4i2.141

Abstract

 ABSTRACTLactic acid bacteria (LAB) were isolated from cocoa beans fermentation Forestero variety from West Sumatera, that were eleven isolates. The isolates were tested to antimicrobial activity against pathogenic bacteria E.coli NBRC 14237, Staphylococcus aureus NBRC 13276, Bacillus subtilis BTCCB 612, listeria m. dan S. Typhii.Results the research showed that, isolates had inhibition zone to pathogenic bacteria, that were 7 mm till 12 mm at 48 hours observation. R2.4 isolate was most potential to inhibition zones growth pathogenic bacteria, That was 11mm till 12 mm to five pathogens. R2.4 isolates was the highest to against pathogenic bacteria (Bacillus subtilis BTCCB, Listeria monocytogenesis and Staphylococcus aureus NBRC) had inhibition zones, that was 12. mm till 48 hours. Listeria monocytogenesis had been known as pest bacterium of food born, so that R2.4 isolate can be used as food biopreservative. Crude of R2.4 isolate molecular weight was 10 kDa by SDS-PAGE.  Key words: Lactic acid bacteria, antimicrobial activity, SDS-PAGE, cocoa fermentation  and food biopreservative    
POTENSI BAKTERI ASAM LAKTAT DALAM MENGHASILKAN BAKTERIOSIN SEBAGAI ANTIMIKROBA DAN PENGUKURAN BERAT MOLEKULNYA DENGAN SDS-PAGE DARI ISOLAT FERMENTASI KAKAO Urnemi -; Sumaryati Syukur; Endang Purwati; Sanusi Ibrahim; Jamsari -
Jurnal Riset Kimia Vol. 1 No. 2 (2008): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v1i2.149

Abstract

ABSTRACT Lactic acid bacteria (LAB) were isolated from of cocoa beans fermentation Forestero variety from West Sumatera, that were eleven isolates. The isolates were tested to antimicrobial activity against pathogenic bacteria E.coli NBRC 14237, Staphylococcus aureus NBRC 13276, Bacillus subtilis BTCCB 612, listeria m. dan S. Typhii. Results the research showed that, isolates had inhibition zone to pathogenic bacteria, that were 7 mm till 12 mm at 48 hours observation. R2.4 isolate was most potential to inhibition zones growth pathogenic bacteria, that was 11mm till 12 mm to five pathogens. R2.4 isolates was the highest to against pathogenic bacteria (Bacillus subtilis BTCCB, Listeria monocytogenesis and Staphylococcus aureus NBRC) had inhibition zones, that was 12.00 mm till 48 hours. Listeria monocytogenesis had been known as pest bacterium of food born, so that R2.4 isolate can be used as food biopreservative. Crude of R2.4 isolate molecular weight was 10 kDa by SDS-PAGE.  Key words: Lactic acid bacteria, Antimicrobial activity, SDS-PAGE, Cocoa fermentation and food biopreservative                                                      
TRANSFORMASI Agrobakterium rhizogenese DAN INDUKSI AKAR RAMBUT PADA TANAMAN KAKAO (Theobroma cacao) UNTUK PRODUKSI SENYAWA ANTIOKSIDAN SECARA INVITRO Sumaryati Syukur; Zozy Aneloi N; Femilya Putri
Jurnal Riset Kimia Vol. 2 No. 2 (2009): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v2i2.156

Abstract

 ABSTRACT Transformation of Ri T-DNA Plasmid Agrobacterium rhizogenese to varieties Theobroma cacao variety TSH which is growing in west Sumatra and induction of hairy roots in order to produce bioflavonoid antioxidant compounds such as, catechin, polyfenol, or monomer and oligomer flavones was successfully obtained. Three spesies of A.rhizogenese (A4,LBA 9457 and ATTCC 15834) originaly from LIPI was used to transform Ri T-DNA plasmid in MS medium via cacao embryo culture. The aim of this paper is to determine the affectivity and ability of the three species of bacterial above to produce hairy roots in cacao invitro culture. The statistical methods RAL was uses with 4 time treatments and 6 time repeated experiments. As treatment was bacterial inoculation and without inoculation as a control. The transformation result shows 2 of 3 of bacterial species have ability to induce hairy roots of.T cacao embryos counting by percentages of explants with producing hairy roots 16.66% for A4, 83.33% for LBA 9547 spesies.qualitative test of polyfenol from hairy roots transformants give (+4) as compared to non transform only (+1). Cathechin compound was determined by spectrophotometer as much as 0.1% for non transform and 0.87 % for hairy roots transformants by LBA 9547. Conformation of plasmid Ri T-DNA hairy roots from two transformants was analysis by PCR methods. The two primers rol B1 (52ATGGATCCCAAATTGCTTCCCCCACGA32) dan rol B2 (53 TTAGG CTTTCATTCGGGTTTACTGCAGC 33) was used. For TR-DNA the primes used is TRI (53 GGAAATTGTGGCGTTGTTGTGGAC 3’) and  TR2 (5’ AATCGTTCAGAGAGCGTCCGA AGTT 3’) . PCR analysis of DNA electrophoresis founded the band of TL region at 780 bp and TR at 1600 bp using DNA Ledder as DNA standard.  Keywords : transformation A.rhizogenese, PCR, Theobroma cacao, kultur embrio, kultur akar rambut, metabolit sekunder, cathechin    
OPTIMASI NUTRISI MEDIA PERTUMBUHAN BAKTERI TERMOFIL PENGHASIL SELULASE DARI SUMBER AIR PANAS RIMBO PANTI Armaini -; Abdi Dharma; Sumaryati Syukur; Jamsari -
Jurnal Riset Kimia Vol. 5 No. 1 (2011): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v5i1.165

Abstract

 Optimization have been done on the media for the growth of the isolated thermophiles bacteria from hot springs Rimbo Panti, the nutrients comprising variety of carbon sources such as CMC (carboxymethyl cellulose), avicell (micro crystalline cellulose), and cellobiose, with a variety of sources organic nitrogen, peptone, extracts yeast, tryptone, and urea, as well as variations consist of inorganic nitrogen sources, KNO3, NaNO3, (NH4)2SO4, and (NH4)NO3. Determination of cellulase activity performed using DNS reagent (3,5-dinitro salicylic acid). Maximum cellulase production with high activity based on the results of this research, the best of carbon source is CMC with optimum concentration 0.125%, inorganic nitrogen source is peptone with the optimum concentration of 0.3 to 0.4% and the inorganic nitrogen source is (NH4)2SO4 with optimum concentration of 0.2 - 0.25%. Optimization of size of inoculums obtained the optimum amount of inoculums 2%. Keywords: Optimization, thermophiles bacteria, cellulose, carbon sources, nitrogen sources
SKRINING DAN IDENTIFIKASI BAKTERI PENDEGRADASI INULIN DARI SUMBER AIR PANAS PADANG BALIMBIANG DI SOLOK Minda Azhar; Sumaryati Syukur; Dessy Natalia; Vovien -; Jamsari -
Jurnal Riset Kimia Vol. 5 No. 1 (2011): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v5i1.175

Abstract

 ABSTRACT Thermophilic bacteria and thermotolerant bacteria are potential sources of thermostable of inulin degradating enzyme, an enzyme which converts inulin into fructose and FOS prebiotics. Isolation and identification of 16S rDNA gene inulin degradation bacteria from hot springs of Padang Balimbiang in Solok have been undertaken. Screening of inulin degradation bacteria was done using direct and undirect methods on medium with inulin or inulin-RBB as a sole carbon source. One inulin degradation bacteria have been obtained from 21 isolates. The isolate was designated as UBCT-030. The isolate is able to grow at temperature 23 °C to 60 °C. According to 16S rDNA gene analysis, phisiology and morphology bacteria on UBCT-030 isolate was identified as Bacillus subtilis.  Keywords: inulinase bacteria, hot springs, Bacillus subtilis, inulin, 16S rDNA gene
Analysis of gene polymorphisms TGF? rs 1058213 to cleft lip cases with or without a non-syndrome cleft palate in Riau Malay Ethnic Masrial Masrial; Yanwirasti Yanwirasti; Sumaryati Syukur; Endang Syamsudin
Journal of Dentomaxillofacial Science Vol. 3 No. 1 (2018): (Available online: 1 April 2018)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v3i1.498

Abstract

Objective: This study aims to determine the role of gene polymorphism TGFα rs 1058213 in cases of cleft lip with or without cleft palate nonsyndrome in the Riau Malay tribe.Material and Methods: DNA extraction of venous blood and polymorphism TGFα rs 1058213 was assessed using the Restriction Fragment Length Polymorphism-PCR (RFLP-PCR) working method and followed by sequencing. Genotyping SNP rs1058213 in the TGFα gene was performed by the RFLP-PCR method in control and CLP NS patients. The data was assessed using the test statistic premises T-test difference test.Results: The total sample size included 68 samples consisting of 34 CLP NS samples and 34 control samples. The cases of CLP NS occurred more in men than women, 20 cases (59%) and 14 cases (14%), respectively.Conclusion: There was no significant difference between TGFα rs 1058213 gene polymorphisms in CLP NS and controls in the Riau Malay tribe.
Co-Authors -, Risfaheri -, Urnemi -, Vovien -, Wiranda Abdi Dharma Allimuddin Tofrizal Armaini - Asmar Yulastri Azhar, Minda Bisping Bernward Bramadi Arya Dahlyanti, Rina Desmiwarti Desmiwarti DESSY NATALIA Endang Purwati RN Endang Syamsudin fahmi, Aliyah - Femilya Putri Ferdinal Ferry Fivi Mona Bareno Helmi Arifin Hermansyah Aziz Hermansyah Aziz Hevi Horiza Hidayat, Zoni Imelda Imelda Jamsari Jamsari Lidya Sari Utami Linda Rosalina Maliza, Rita Masrial Masrial Melia, Sri Minda Azhar Murni, Yulia Nanda Tri Wahdini Natalia, Dessy Norman Ferdinal Novesar Jamarun Novesar Jamarun Putri Zelfitri Zen Rahmiana Zein Refilda Refilda Refinel - Rina Dahlyanti Risfaheri - Risma Sari Safni Safni Sanusi Ibrahim Sartika, Diza Sumanti, Titi Suryati Suryati Syukri Arief Titi Sumanti Tommi Hermansyah Urnemi - Urnemi Urnemi Vovien - Wardi, Epi Supri Wiranda - Yanwirasti Yanwirasti Yefrida Yefrida Yetria Rilda Yulia Murni Yulizar Yusuf Zilfa Zilfa ZK Abdurahman Baizal Zona Octarya Zona Octarya Zoni Hidayat Zozy Aneloi Noli Zulkarnain Chaidir Zulkarnain Khaidir