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Journal : Jurnal Agroteknos

KARAKTERISASI MORFOLOGIS Trichoderma spp. INDIGENUS SULAWESI TENGGARA GUSNAWATY HS -; MUHAMMAD TAUFIK; LENI TRIANA; ASNIAH -
Jurnal Agroteknos Vol 4, No 2 (2014)
Publisher : Jurnal Agroteknos

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Abstract

This study aimed to determine differences in the morphological characteristics of isolates of Trichoderma spp indigenous of Southeast Sulawesi. The experiment was conducted at the Laboratory of Agro Technology, Unit of Plant Pest and Disease, Faculty of Agriculture, University of Halu Oleo, Kendari.  This study used 11 isolates of trichoderm indegenous of Southeast Sulawesi. Observation variables were macroscopic characteristics, including: colony color and form, and microscopic characteristics, including: form of conidiophores, fialid and and conidia.  The research results showed that the 11 isolates of Trichoderma spp indigenous of Southeast Sulawesi had different morphological characteristics. Types of  Trichoderma spp obtained out of the 11 isolates were T. hamantum, T. koningii, T. harzianum, T. polysporum and T. aureoviride. Keywords : characterization, indigenous Southeast of Sulawesi, Trichoderma spp.
EFEKTIFITAS TRICHODERMA INDIGENUS SULAWESI TENGGARA SEBAGAI BIOFUNGISIDA TERHADAP Colletotrichum sp. SECARA IN- VITRO HS GUSNAWATY; MUHAMMAD TAUFIK; HERMAN HERMAN
Jurnal Agroteknos Vol 4, No 1 (2014)
Publisher : Jurnal Agroteknos

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Abstract

This research aimed to determine the effectivity of Trichoderm isolates indigenous of Southeast Sulawesi as biofungicide to Colletotrichum sp  in- vitro. This research was carried out in the Laboratory of Agrotechnology, Plant Pathology unit, Faculty of Agriculture, Halu Oleo University.  Inhibition effectivity of 11  Trichoderma  spp.  isolates was done in double PDA media.  This research used  the complete randomized design (CRD).  The observed variables were the inhibitory of  Trichoderma  spp.  indigenous on the growth of Colletotrichum sp. Research result showed that the 11 isolates were effective as biofungicide against Colletotrichum sp.  The data analysis showed that isolates DPA was the best isolate, inhibiting  Colletotrichum  sp. by 77.69%, otherwise LKP was isolate with the lowest inhibitory ability against  Colletotrichum  sp, by 65.07%.  Inhibition mechanism of isolates DPA and DKT against Colletotrichum sp was by area competition and parasitism.Key words:  Antagonist, Colletotrichum sp., Trichoderma Indigenous.
UJI KISARAN INANG POTYVIRUS PENYEBAB MOSAIK NILAM (Pogostemon cablin (Blanco) Benth) ASAL SULAWESI TENGGARA ASMAR HASAN; MUHAMMAD TAUFIK; GUSNAWATY HS -; SARAWA -
Jurnal Agroteknos Vol 4, No 3 (2014)
Publisher : Jurnal Agroteknos

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Abstract

The objective of the research was to determine the host alternate of Potyvirus in Southeast Sulawesi using a host range technique. Observed variables were incubation period, disease incidence, and variation symptom. The research results showed that virus isolates could be mechanically transmitted to melon plant (Cucumismelo) but showing no external symptoms while on tomato (Solanum lycopersicum), long-beans (Vigna sinensis), tobacco (Nicotiana tabacum and Nicotiana benthamiana), swamp-land (Ipomoea reptana), red-amaranth (Alternantheraamoena), chili (Capsicum annum), and egg plant (Solanum melongena) so far the existence of the Potyvirus could not be detected after mechanically inoculated.
EFEKTIVITAS MINYAK CENGKEH TERHADAP PATOGEN YANG BERASOSIASI DENGAN TANAMAN TOMAT (Lycopersicum esculentum Mill.) PUTRI MEGAYANTI PADDA; MUHAMMAD TAUFIK; ASNIAH ASNIAH; MUHAMMAD BOTEK; ABDUL RAHMAN; GUSNAWATY HS GUNAWATY; MARIADI MARIADI
Jurnal Agroteknos Vol 12, No 1 (2022)
Publisher : Jurnal Agroteknos

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Abstract

This study aimed to evaluate the effect of clove oil to control pathogens associated with tomato plants in screen houses. The study was carried out using a completely randomized design (CRD) with 5 treatments repeated 3 times and each treatment consisted of 3 plants for a total of 45 plants, the treatments given were P0 (without application), P1 (pathogenic fungus Sclerotium sp. without clove oil application). , P2 (pathogenic fungus Sclerotium sp. with clove oil application), P3 (pathogenic fungus Helminthosporium sp. without clove oil application), P4 (pathogenic fungus Helminthosporium sp. clove oil application). The variables observed were plant height, stem diameter, number of branches and disease severity of tomato plants. The results showed that clove oil was able to increase plant growth as indicated by the highest average height and diameter of 18.61cm and 0.31cm, respectively. Clove oil application also suppressed the development of the pathogen Sclerotium sp. As indicated by the average incubation period and disease severity 18.67 days and 6%, respectively.
DETEKSI POTYVIRUS PADA NILAM (Pogostemon Cablin (BLANCO) BENTH) DENGAN TEKNIK ELISA DI SULAWESI TENGGARA MUHAMMAD TAUFIK; ASMAR HASAN; ANDI KHAERUNI; GUSNAWATY HS - -; SARAWA MAMMA
Jurnal Agroteknos Vol 4, No 1 (2014)
Publisher : Jurnal Agroteknos

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Abstract

Mosaic symptoms were observed on Patchouli (Pogostemon cablin) around North Kolaka and Kolaka, Southeast Sulawesi during surveys in early 2012. Indirect-ELISA based detection was conducted using symptomatic leaf samples. The objective of the research was to determine disease incidence of Potyvirus in several farms of Patchouli plant in Southeast Sulawesi. The results showed that Patchouli plant (Pogostemon cablin) was found to be infected with Potyvirus disease showing mosaic symptoms and malformation on the leaf samples i.e. in Amotowo and Boro-Boro Villages of subdistrict Boro-Boro, and Landabaro Village, Mowila subdistrict of South Konawe regency; Asinua Village of subdistrict Unaaha, Lambuya district of subdistrict Lambuya, and Bungguosu district, Konawe subdistrict of Konawe regency; and Anduonohu district, Poasia subdistrict of Kendari regency. This is the first report on Potyvirus infection on patchouli in Southeast Sulawesi. Keywords: Inderect-ELISA, mosaic, Potyvirus, Pogostemon cablin
PENGGUNAAN Bacillus spp. SEBAGAI AGENS BIOKONTROL UNTUK MENGENDALIKAN PENYAKIT LAYU FUSARIUM PADA TANAMAN CABAI Andi Khaeruni; HS Gusnawaty
Jurnal Agroteknos Vol 2, No 3 (2012)
Publisher : Fakultas Pertanian Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56189/ja.v2i3.2280

Abstract

The use of Bacillus spp. Bacteria as biocontrol agents is one alternative disease control for Fusarium wilt Fusarium oxysporum f.sp. Lycopersici on chilli plant . this study aimed to determine the effectiveness of Bacillus spp. As a biocontrol agent in various ways of application to control Fusarium wilt disease in pepper in in-vivo. Experiments were prepared  using  two  factor  factorial  in  a  completely randomized design  (CRD).  Factor  1 (Applications) consisted of levels, namely: application of biocontrol agent Bacillus spp. M11 isolates (B1), application of biocontrol agents Bacillus spp. isolates F133 (B2), and application biocontrol agent Bacillus spp. mix. M11 isolates and isolates F133 (B3). The second factor (time of inoculation/application) consisted 3 levels, namely: inoculating the seed (S1), inoculation of Bacillus spp. simultaneously with pathogen inoculation (S2), inoculation of Bacillus spp.  one week after pathogen inoculation (S3), to see the effect of the two isolates of Bacillus spp. that the latent period, the biocontrol agents Bacillus spp. mix isolates M11 and F133, was the best treatment with the longest latency period of the disease (16 hsi), the best plant growth by plant height reached 57,39 cm, and the lowest disease incidence and severity were 12,96 % and 11,11 %, respectively interaction between agent Bacillus spp. mix. Isolates M11 and F133 at the time of application on seed significantly affected the incidence of disease and plat height. Keyword: biocontrol agents, Bacillus spp., and Fusarium oxysporum f.sp. Lycopersici
EVALUASI KETAHANAN KULTIVAR SORGUM TERHADAP PENYAKIT LAYU FUSARIUM Asniah Asniah; HS Gusnawaty; Muhammad Taufik
Jurnal Agroteknos Vol 3, No 2 (2013)
Publisher : Fakultas Pertanian Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56189/ja.v3i2.2314

Abstract

This study aimed to evaluate the resistance of sorghum cultivars to wilt fusarium. This research was arranged in a randomized block design, with six  treatments [brown  sorghum, white sorghum and  red  sorghum with no inoculation fusarium, brown    sorghum, white sorghum  and  red  sorghum with  inoculation  fusarium]  with  3  (three)  replications. Data observed were plant height, leaf number, leaf area, and heaviest of grain weight for vegetative observation, while for disease character was disease incidence. The results showed that none of sorghum cultivars evaluated showed resistance to wilt fusarium infection. The tolerant response to wilt fusarium  was observed on brown sorghum, whereas red and white sorghum were  susceptible to wilt fusarium. The heaviest of  grain weight was found in brown sorghum (27.86 g/penicle). The wilt fusarium caused disease incidences ranging from 33.33% (brown sorghum)  to 50% (red sorghum). Keywords: cultivars, disease incidence, fusarium, sorghum, resistance
RESPON KETAHANAN BERBAGAI VARIETAS TOMAT TERHADAP PENYAKIT LAYU BAKTERI (Ralstonia solanacearum) Adriani Adriani; Abdul Rahman; HS Gusnawaty; Andi Khaeruni
Jurnal Agroteknos Vol 2, No 2 (2012)
Publisher : Fakultas Pertanian Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56189/ja.v2i2.2263

Abstract

The study was aimed to  know the resistance response of some tomato varities to bacterial wilt disease (Ralstonia solanacearum). The study was arranged in a completely randomized design (CRD) with six tomato variety treatmens, namely : Permata, Cosmonot, Idola, Gress, Ratna, and a local Muna variety. Each treatment had three replicates of six plants. The resistance response observed were : the latent period, disease incidence, disease severity,  and  plant  height.  The  response  was   observed  weekly  until  7   weeks  after inoculation.  The  results  showed  that  Cosmonot  variety  was  resistant  to  bacterial  wilt disease, without any disease incedence occured and the disease severity was only 6,66%. Whereas the Local Muna variety as well as Permata, Gress, and Idola were susceptible to bacterial wilt disease. On the other hand, Ratna variety showed slight tolerant response. Keywords : bacterial wilt, Ralstonia solanacearum, resistance reaction, tomato variety
PENGARUH FUNGI MIKORIZA ARBUSKULA DAN NUTRISI ORGANIK TERHADAP PERTUMBUHAN TANAMAN CABAI MERAH BESAR (Capsicum annuum L.) Makmur Jaya Arma; Risnawati Risnawati; HS Gusnawaty
Jurnal Agroteknos Vol 3, No 3 (2013)
Publisher : Fakultas Pertanian Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56189/ja.v3i3.2321

Abstract

The research to study the effects of arbuscular mycorrhiza fungi (AMF) and organic nutrients to enhance the growth of chili has been conducted in Experimental Field, Faculty of Animal Husbandry and laboratory of Agrotechnology, Halu Oleo University, from June to November 2012.  The research was based on the split-plot design with a randomized  block design pattern (RAK) of two factors: Organic Nutrition as the main plot and AMF as subplot. Organic nutrients as the main plot consisted of three levels, namely: without organic nutrition (S0), 1 mL L-1  of water (S1) and 2 mL L -1  of water (S2);   and AMF dose as subplot consisted of three levels, namely: without AMF (M0), 5 g plant-1  (M1) and 10 g plant-1  (M2). therefore, there were 9 combinations of treatments and each treatment combination was repeated  three  times  to  obtain  27  experimental  units.  Each  variable  was  analyzed  by analysis of variance, then followed by Duncan's Multiple Range Test (UJBD) at 95% confidence level. The results of research indicated that the best interaction of   AMF   and organic  nutrient  treatment was 10 g AMF plant-1 (M2) and 2 mL L-1 (S2) of organic nutrients. This treatment combination can improve growth on variables: leaf area, leaf area index and yield index of the chili plants.  The best treatment for AMF independently was at 10 g plant-1 (M2) because it can promoted growth of plant height of the chili plants. The best treatment for organic matter independently was at 2 mL L-1(S2), because it can promoted growth of plant height of the chili plants. Keywords: FMA, organic nutrition, growth, chili
UJI EFEKTIVITAS BEBERAPA MEDIA UNTUK PERBANYAKAN AGENS HAYATI Gliocladium sp. HS Gusnawaty; Muhammad Taufik; Edi Wahyudin
Jurnal Agroteknos Vol 3, No 2 (2013)
Publisher : Fakultas Pertanian Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56189/ja.v3i2.2312

Abstract

This research was carried out in the Laboratory of Pests and Disease, Agricultural Faculty  of  Haluoleo  University,  from  October  to  December  2012.  This  research  was arranged   in   A   Completely   Randomized   Design   (CRD),   with   7   treatments,   namely: Gliocladium sp. in sago waste (a), Gliocladium sp. in cashew seed wastes (b), gliocladium sp. in saw dust waste (c), Gliocladium sp. in corn medium (d), Gliocladium sp. in bran medium (e),  Gliocladium  sp.  in rice  medium  (f) and  gliocladium sp.  in rice  husk  waste  (g).   The research results showed that media used had different effectiveness.   The most effective medium for Gliocladium sp. Propagation was rice bran medium, based on Gliocladium sp. growth (100%), and the number of Gliocladium sp. conidium ( 2,0 x105/g). Keywords: effectiveness, Gliocladium sp., medium, propagation