Prihartini Widiyanti
Department Of Physics, Science & Technology, Faculty Airlangga University, Surabaya, Indonesia Institute Of Tropical Disease Airlangga University, Surabaya, Indonesia

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The Utilization of Achatina fulica Mucus in Alginate Membrane as Wound Healing Accelerator and Anti- Infection Material Widiyanti, Prihartini; Putra, Alfian Pramudita; Kuntjoro, Eko Budi; Rahmawati, Fatkhunisa; Adhitioso, Satrio; Mayasari, Dita Ayu
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 1 (2014)
Publisher : Institute of Topical Disease

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Abstract

Wound should be covered with bandage that is called wound dressing. Most people use synthetic materials such as gauze dressing. Gauze has high absorption of NaCl, which is often used to cleanse the wound. However, discomfort and pain arise since the gauze becomes sticky on the wound. Therefore, we need other alternatives instead of gauze to cover wound. One such alternative is the alginate membrane. This study used alginate membrane with mixture of mucous of the snail Achatina fulica, which contain proteins such as proline, serine asparagine, glycosaminoglycan, hydroxylysine, trionin and so forth, to activate the growth factor. Alginatepowder and carboxymethl cellulose (CMC) was dissolved in distilled water mixed with mucus of the snail Achatina fulica in four variations (4:0; 4:1, 4:2, 4:3) through a magnetic stirrer, and casted on a baking sheet covered with sterile gauze. High Performance Liquid Chromatography (HPLC) test showed that the glycosaminoglycan content was found on the mucous of Achatina fulica. This was indicated by the appearance of peak at 325–350 second. The most optimum alginate and mucus composition was in ratio of 4:2. This ratio resulted in a wound dressing that was still able to absorb the exudate and optimally accelerated wound healing.
TUBERCULOSIS COUNTER (TC) AS THE EQUIPMENT TO MEASURE THE LEVEL OF TB IN SPUTUM Widiyanti, Prihartini; Sasmito, Dyar; Fibriawan, Yufan; Rahmawati, Fatkhunisa; Purwanda, Fendy
Indonesian Journal of Tropical and Infectious Disease Vol 3, No 2 (2012)
Publisher : Institute of Topical Disease

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Abstract

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. This disease is the third killer disease aftercardiovascular diseases and respiratory diseases, and is also he number one killer disease in a group of infectious diseases. This is partly due to the late handling and a non real time detection, both of which will inhibit the therapy which yields a large numberof microorganisms in the body, and will eventually complicate the recovery. Based on this phenomenon, we offered an alternativesolution for detecting the sum of microorganism using Tuberculosis Counter, a tool used to count the number of Tuberculosis bacteria in the patients sputum. Technically, the patients sputum preparat was screened using the TCS230 color sensor that was able to filter the color of the preparat. Tuberculosis bacteria in the stained sputum Ziehl-Niellsen preparat was colored red, while the other was colored blue. By utilizing these optical phenomena, the TCS230 color sensor was supposed to filter the red color in the preparat. By using regression equation measurement, we gained the equation which then correlated the bit value as an output of the sensor with the number of Tuberculosis bacteria. Then, the digitalization process yielded the real time and accurate data of Tuberculosis bacteria.
COLOSTRUM-COLLAGEN-HYDROXYAPATITE COMPOSITE, AN EXCELLENT CANDIDATE BIOMATERIAL FOR BONE REPAIR AND BONE INFECTION MANAGEMENT Purnobasuki, Heri; Widiyanti, Prihartini; Fiddarain, Nilna Faizah; Anwar, Mirzaq Hussein; Putri, Kholifatul Wanda; Setiawan, Dio Nurdin
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 2 (2014)
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Abstract

In the case of bone fracture or defect after surgery, which is common in patients with bone cancer (osteosarcoma), it takes a long time for closure and it may cause an infection problem. The use of collagen-hydroxyapatite composite with a blend of colostrum as a scaffold is aimed to accelerate the process of osteoblast growth, inhibite the emergence of infections, and act as bone tissue repair material. The method used was the hydrogel formation process and freeze dry process to remove the solvent and to form pores. The composition of scaffold composite manufactured was 15% collagen, 75% hydroxyapatite and 10% colostrum. Combination of scaffold collagen-hydroxyapatite-colostrum has quite reliable properties because SEM test showed that scaffold could bind to both and could bind to both and could form sufficient pores to provide enough place for bone cells (osteoblats) to grow. The results of MTT assay revealed percentage of above 60%, which indicates that the material is not toxic. In conclusion, collagen-hydroxyapatite-colostrum combination is an excellent biomaterial candidate for bone repair and bone infection management.
USING LEARNING VECTOR QUANTIZATION METHOD FOR AUTOMATED IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS Widiyanti, Prihartini; Purwanti, Endah
Indonesian Journal of Tropical and Infectious Disease Vol 3, No 1 (2012)
Publisher : Institute of Topical Disease

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Abstract

In this paper, we are developing an automated method for the detection of tubercle bacilli in clinical specimens, principally the sputum. This investigation is the first attempt to automatically identify TB bacilli in sputum using image processing and learning vector quantization (LVQ) techniques. The evaluation of the learning vector quantization (LVQ) was carried out on Tuberculosis dataset show that average of accuracy is 91,33%.
The Prevalence of Human Immunodefiency Virus-1 (HIV-1) Subtypes and Transmission Method among HIV/AIDS Infection Patient in Tulungagung, East Java Indonesia Nasronudin, Nasronudin; Lusida, Maria Inge; Witaningrum, Adiana Mutamsari; Kotaki, Tomohiro; Khairunisa, Siti Qamariyah; Utomo, Budi; Widiyanti, Prihartini; Rahayu, Retno Pudji; Juniastuti, Juniastuti; Qushay, M.; Ardianto, Achmad
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 5 (2015)
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Abstract

The rapid epidemic growth of HIV is continuing in Indonesia. There are some factors which have influenced the spreading of this epidemic in Indonesia, such as the poor awareness to avoid unsafe free sex attitude and the sharing of needles and syringes among intravenous drug users (IDUs). The sexual transmission of HIV has also apparently increased in Tulungagung. Commercial sex workers play a significant role in the spread of HIV in Tulungagung. People in Tulungagung have worked at other countries as Indonesian migrants. This condition can cause the increase number of HIV-1 case and the possibility of genetic variation (subtype) HIV-1 in Tulungagung. This research is aimed to analyze the subtype and to determine estimation of transmission mode on infected patient of HIV-1 and AIDS who came to Seruni clinic Dr. Iskak hospital in Tulungagung. 40 HIV?AIDSpatients were interviewed to determine the subtype and the transmission mode. The results showed that 14 of 40 plasma samples (35%) were successfully to amplified and sequenced. OverallCRF01-AE wereidentified as predominant subtype among HIV/AIDS patients in Tulungagung. Based on individual information, 31 of 40 subjects (77%) were heterosexual transmission.
THE ROLE OF HYPERBARIC THERAPY IN THE GROWTH OF CANDIDA ALBICANS Widiyanti, Prihartini
Indonesian Journal of Tropical and Infectious Disease Vol 4, No 4 (2013)
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Abstract

Background: Candida albicans is opportunistic pathogen fungi which cause many disease in human such as reccurrent apthous stomatitis, skin lesions, vulvavaginitis, candiduria and gastrointestinal candidiasis. Aim: Infection mechanism of C. albicans is very complex including adhesion and invasion, morphology alteration from khamir form cell to filamen form (hifa), biofilm forming and the avoidance of host immunity. Method: The ability of C. albicans to adhere to the host cell which is act as important factor in the early colonization and infection. Result: The phenotype alteration to be filament form let the C. albicans to penetrate to the epithelium and play important role in infection and separation C. Albicans to the host cell. Hyperbaric oxygen is the inhalation of 100 percent oxygen inside hyperbaric chamber that is pressurized to greater than 1 atmosphere (atm). Conclusion: The organism was found to be inhibited within a pressure/time range well tolerated by human subjects, suggesting that hyperbaric oxygen might be used successfully in treating human candidiasis.
DIGITAL DETECTION SYSTEM DESIGN OF MYCOBACTERIUM TUBERCULOSIS THROUGH EXTRACTION OF SPUTUM IMAGE USING NEURAL NETWORK METHOD Apsari, Retna; Widiyanti, Prihartini; Arisgraha, Franky
Indonesian Journal of Tropical and Infectious Disease Vol 3, No 1 (2012)
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Abstract

Tuberculosis (TBC) is an dangerous disease and many people has been infected. One of many important steps to control TBC effectively and efficiently is by increasing case finding using right method and accurate diagnostic. One of them is to detect Mycobacterium Tuberculosis inside sputum. Conventional detection of Mycobacterium Tuberculosis inside sputum can need a lot of time, so digitallydetection method of Mycobacterium Tuberculosis was designed as an effort to get better result of detection. This method was designed by using combination between digital image processing method and Neural Network method. From testing report that was done, Mycobacterium can be detected with successful value reach 77.5% and training error less than 5%.
The Effect of Gendarussin a Isolates of Justicia gendarussa Burm.f. Leaf in Reverse Transcriptase Inhibition of HIV Type I In Vitro Nasronudin, Nasronudin; Aksono, Bimo; Widiyanti, Prihartini; E. W., Bambang Prajogo
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 5 (2015)
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Abstract

Screening has been done to a few extracts from the leaves Justicia gendarussa Burm.f to see the growth rate of the virus from the blood plasma of HIV patients at Dr Soetomo Hospital. It is known that J. gendarussa leaf extract inhibits HIV type 1 reverse transcriptase. In addition, its main content is gendarussin A, besides gendarussin B, JGF1, JGF2 and JGF3, which have just identified. At the beginning, extraction and fractionation were performed with 3 models that highlight the absolute methanol, 70% methanol and 70% ethanol with the release of alkaloids. Furthermore, samples of each fraction were incubated in plasma of HIV patients with a titer of 3.6 10^6 copies for 1 h in concentrations of 1.64 ppm, 4.1 ppm, 8.2 ppm, 16.4 ppm and 41.0 ppm. After incubation, examination was performed by using Nucli sens a machine, which is a combination of PCR and Elisa, thus avoiding direct contact with the highly pathogenic virus. The result showed that the activity sequence from the most potential to the weak, among others, was 1.64 ppm >4.1 ppm > 8.2 ppm > 16.4 ppm > 41.0 ppm, each with barriers value of 0.62 10^6, 1.4 10^6, 1.6 10^6, 2.4 10 cells/ml. In conclusion, highest anti-HIV activity comes from the concentration of gendarussin A isolate at 1.64 ppm. Furthermore, after linearregression of y = -3.063 x + 81.37 was done, the IC50 of 10.24 ppm was obtained.
BASIC MECHANISM OF HYPERBARIC OXYGEN IN INFECTIOUS DISEASE Widiyanti, Prihartini
Indonesian Journal of Tropical and Infectious Disease Vol 2, No 1 (2011)
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Abstract

Hyperbaric oxygen therapy (HBOT) is the inhalation of 100 percent oxygen inside a hyperbaric chamber that is pressurized to greater than 1 atmosphere (atm). HBOT causes both mechanical and physiologic effects by inducing a state of increased pressure and hyperoxia. HBOT is typically administered at 1 to 3 atm. While the duration of an HBOT session is typically 90 to 120 minutes, the duration, frequency, and cumulative number of sessions have not been standardized. HBO has been use widely in treating gangrene diabetic, stroke, osteomyelitis and accelerating wound healing. The use of HBO in infectious disease is wide, so the mechanism of hyperbaric oxygen in infectious disease should be well-understand. This understanding could bring the proper and wise management of infectious disease and to prevent the side effect of each therapy.
Profile of Hematocrit Level Captured by Digital Hematocrit Test Widiyanti, Prihartini; Sardjono, Tri Arif
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 6 (2015)
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Abstract

The dengue fever is a disease caused by Dengue virus which is transmitted via Aedes aegypti and Aedes albopictus vector. This Dengue Haemorrhagic Fever (DHF) case in Indonesia tend to rise from year to year caused by delayed detection and inadequate handling. The laboratory parameter of hematocrite had regularly been performed using invasive method by taking the blood from the patient. This method is still not been able to monitor patients with DHF by repetitive and accurate measurament. This research project aims is to perform a digital hematocrit test (DHT) with non-invasive accurate sensors. Digital Hematocrit Test (DHT) is needed to presenting fast, exact, economical and accurate detection methods of hematocrit level. Measureable magnitude by the instrumentation is non-absorb intensity electromagnetic waves 560 nm emitted by transmitter captured by receiver. Signal captured by the receiver then converted into electrical signal. The electrical signal from receiver was the levels of hemoglobin. Levels of hemoglobin then converted to hematocrit. Hematokrit is three times the level of hemoglobin. Technology of hematocrit monitoring is aimed to control DHF patient clinical symptoms continuously and acquisitively.
Co-Authors Achmad Ardianto Achmad Ardianto, Achmad Adanti Wido Paramadini Adiana Mutamsari Adiana Mutamsari Witaningrum, Adiana Mutamsari Agung Prabowo Ahmad Fadli Alfian Pramudita Putra Andini Isfandiary Bambang Prajogo Bambang Prajogo Eko Wardoyo Bella Wahyu Sukma Wati Bimo Aksono Bimo Aksono Bimo Dwi Lukito Bimo Dwi Lukito, Bimo Dwi Bramantono - Budi Utomo Budi Utomo Cindy Narita Kusuma Astuti DENI NOVIANA Dina Kartika Putri Dio Nurdin Setiawan Dio Nurdin Setiawan, Dio Nurdin Dita Ayu Mayasari Dita Ayu Mayasari, Dita Ayu Djony Izak Rudyardjo Dyar Sasmito Dyar Sasmito, Dyar Eko Budi Kuntjoro Eko Budi Kuntjoro, Eko Budi Endah Purwanti Fatkhunisa Rahmawati Fatkhunisa Rahmawati Fatkhunisa Rahmawati, Fatkhunisa Fa’ilal Chusna Fendy Purwanda Fendy Purwanda, Fendy Ferdiansyah Ferdiansyah Ferdiansyah Ferdiansyah Fitriyatul Qulub Fitriyatul Qulub Franky Arisgraha Franky Arisgraha, Franky Ganis Tjahjono Hafrizal Riza Hafrizal Riza, Hafrizal Hajria Jabbar Hendita Nur Maulida Heni Ismawati Heri Purnobasuki Heri Purnobasuki, Heri Herry Wibowo Jan Ady Julieta Kartikasari Juniastuti Juniastuti Juniastuti Juniastuti Kholifatul Wanda Putri Kholifatul Wanda Putri, Kholifatul Wanda Kirana Anugerah Putri Ludita Woro Indrio M. Qushay M. Qushay, M. Maria Inge Lusida Maria Inge Lusida Mirzaq Hussein Anwar Mirzaq Hussein Anwar, Mirzaq Hussein Muhammad Vitanata Arfijanto Nabila Safira Khairina Nasronudin Nasronudin Nasronudin Nasronudin Nasronudin Nasronudin Nasronudin Nasronudin Nasronudin Nasronudin Ni Putu Ermi Hikmawati Ni Putu Ermi Hikmawati, Ni Putu Ermi Nilna Faizah Fiddarain Nilna Faizah Fiddarain, Nilna Faizah Radicha Arnanda Putri Indrawati Retna Apsari Retno Indrawati Roestamadji Retno Pudji Rahayu Satrio Adhitioso Satrio Adhitioso Sherina Pradita Eka Wahyudi Siswanto Siswanto Siti Irma Mashitah Siti Qamariyah Khairunisa Siti Qamariyah Khairunisa, Siti Qamariyah Siti Qomariyah Khairunisa Siti Qomariyah Khairunisa, Siti Qomariyah Tia Rahayu Wijayanti Tomohiro Kotaki Tomohiro Kotaki Tomohiro Kotaki, Tomohiro Tri Arif Sardjono Tri Arif Sardjono, Tri Arif Tria Refi Indriani Veren Audia Nurmansya Wulandari Prima Devi Yufan Fibriawan Yufan Fibriawan, Yufan