Garuda - Garba Rujukan Digital

p-Index From 2019 - 2024

2.195

P-Index

This Author published in this journals

All Journal Jurnal Sains Materi Indonesia Folia Medica Indonesiana Indonesian Journal of Tropical and Infectious Disease MEDISAINS Dental Journal (Majalah Kedokteran Gigi) Indonesian Journal of Chemistry Jurnal Layanan Masyarakat (Journal of Public Service) Indian Journal of Forensic Medicine & Toxicology I-Com : Indonesian Community Journal Jurnal Rekayasa Kimia & Lingkungan Prosiding Seminar Nasional Unimus

Prihartini Widiyanti

Biomedical Engineering Study Program, Department Of Physics, Faculty Of Science And Technology, Universitas Airlangga Institute Of Tropical Disease, Universitas Airlangga

Author-ID : 508655

Agriculture, Biological Sciences & Forestry Humanities Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Engineering Environmental Science Health Professions Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

Published : 43 Documents Claim Missing Document

Claim Missing Document

Articles

1 2 3 4 5

TUBERCULOSIS COUNTER (TC) AS THE EQUIPMENT TO MEASURE THE LEVEL OF TB IN SPUTUM Fendy Purwanda; Yufan Fibriawan; Dyar Sasmito; Fatkhunisa Rahmawati; Prihartini Widiyanti
Indonesian Journal of Tropical and Infectious Disease Vol. 3 No. 2 (2012)
Publisher : Institute of Topical Disease Universitas Airlangga

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. This disease is the third killer disease aftercardiovascular diseases and respiratory diseases, and is also he number one killer disease in a group of infectious diseases. This is partly due to the late handling and a non real time detection, both of which will inhibit the therapy which yields a large numberof microorganisms in the body, and will eventually complicate the recovery. Based on this phenomenon, we offered an alternativesolution for detecting the sum of microorganism using Tuberculosis Counter, a tool used to count the number of Tuberculosis bacteria in the patient's sputum. Technically, the patient's sputum preparat was screened using the TCS230 color sensor that was able to filter the color of the preparat. Tuberculosis bacteria in the stained sputum Ziehl-Niellsen preparat was colored red, while the other was colored blue. By utilizing these optical phenomena, the TCS230 color sensor was supposed to filter the red color in the preparat. By using regression equation measurement, we gained the equation which then correlated the bit value as an output of the sensor with the number of Tuberculosis bacteria. Then, the digitalization process yielded the real time and accurate data of Tuberculosis bacteria.

The Utilization of Achatina fulica Mucus in Alginate Membrane as Wound Healing Accelerator and Anti- Infection Material Fatkhunisa Rahmawati; Dita Ayu Mayasari; Satrio Adhitioso; Alfian Pramudita Putra; Eko Budi Kuntjoro; Prihartini Widiyanti
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 1 (2014)
Publisher : Institute of Topical Disease Universitas Airlangga

Wound should be covered with bandage that is called wound dressing. Most people use synthetic materials such as gauze dressing. Gauze has high absorption of NaCl, which is often used to cleanse the wound. However, discomfort and pain arise since the gauze becomes sticky on the wound. Therefore, we need other alternatives instead of gauze to cover wound. One such alternative is the alginate membrane. This study used alginate membrane with mixture of mucous of the snail Achatina fulica, which contain proteins such as proline, serine asparagine, glycosaminoglycan, hydroxylysine, trionin and so forth, to activate the growth factor. Alginatepowder and carboxymethl cellulose (CMC) was dissolved in distilled water mixed with mucus of the snail Achatina fulica in four variations (4:0; 4:1, 4:2, 4:3) through a magnetic stirrer, and casted on a baking sheet covered with sterile gauze. High Performance Liquid Chromatography (HPLC) test showed that the glycosaminoglycan content was found on the mucous of Achatina fulica. This was indicated by the appearance of peak at 325–350 second. The most optimum alginate and mucus composition was in ratio of 4:2. This ratio resulted in a wound dressing that was still able to absorb the exudate and optimally accelerated wound healing.

COLOSTRUM-COLLAGEN-HYDROXYAPATITE COMPOSITE, AN EXCELLENT CANDIDATE BIOMATERIAL FOR BONE REPAIR AND BONE INFECTION MANAGEMENT Dio Nurdin Setiawan; Mirzaq Hussein Anwar; Kholifatul Wanda Putri; Nilna Faizah Fiddarain; Prihartini Widiyanti; Heri Purnobasuki
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 2 (2014)
Publisher : Institute of Topical Disease Universitas Airlangga

In the case of bone fracture or defect after surgery, which is common in patients with bone cancer (osteosarcoma), it takes a long time for closure and it may cause an infection problem. The use of collagen-hydroxyapatite composite with a blend of colostrum as a scaffold is aimed to accelerate the process of osteoblast growth, inhibite the emergence of infections, and act as bone tissue repair material. The method used was the hydrogel formation process and freeze dry process to remove the solvent and to form pores. The composition of scaffold composite manufactured was 15% collagen, 75% hydroxyapatite and 10% colostrum. Combination of scaffold collagen-hydroxyapatite-colostrum has quite reliable properties because SEM test showed that scaffold could bind to both and could bind to both and could form sufficient pores to provide enough place for bone cells (osteoblats) to grow. The results of MTT assay revealed percentage of above 60%, which indicates that the material is not toxic. In conclusion, collagen-hydroxyapatite-colostrum combination is an excellent biomaterial candidate for bone repair and bone infection management.

THE ROLE OF HYPERBARIC THERAPY IN THE GROWTH OF CANDIDA ALBICANS Prihartini Widiyanti
Indonesian Journal of Tropical and Infectious Disease Vol. 4 No. 4 (2013)
Publisher : Institute of Topical Disease Universitas Airlangga

Background: Candida albicans is opportunistic pathogen fungi which cause many disease in human such as reccurrent apthous stomatitis, skin lesions, vulvavaginitis, candiduria and gastrointestinal candidiasis. Aim: Infection mechanism of C. albicans is very complex including adhesion and invasion, morphology alteration from khamir form cell to filamen form (hifa), biofilm forming and the avoidance of host immunity. Method: The ability of C. albicans to adhere to the host cell which is act as important factor in the early colonization and infection. Result: The phenotype alteration to be filament form let the C. albicans to penetrate to the epithelium and play important role in infection and separation C. Albicans to the host cell. Hyperbaric oxygen is the inhalation of 100 percent oxygen inside hyperbaric chamber that is pressurized to greater than 1 atmosphere (atm). Conclusion: The organism was found to be inhibited within a pressure/time range well tolerated by human subjects, suggesting that hyperbaric oxygen might be used successfully in treating human candidiasis.

THE PREVALENCE OF HUMAN IMMUNODEFIENCY VIRUS-1 (HIV-1) SUBTYPES AND TRANSMISSION METHOD AMONG HIV/AIDS INFECTION PATIENT IN TULUNGAGUNG, EAST JAVA INDONESIA Achmad Ardianto; Siti Qamariyah Khairunisa; Tomohiro Kotaki; Adiana Mutamsari Witaningrum; M. Qushay; Juniastuti Juniastuti; Retno Pudji Rahayu; Prihartini Widiyanti; Budi Utomo; Maria Inge Lusida; Nasronudin Nasronudin
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 5 (2015)
Publisher : Institute of Topical Disease Universitas Airlangga

The rapid epidemic growth of HIV is continuing in Indonesia. There are some factors which have influenced the spreading of this epidemic in Indonesia, such as the poor awareness to avoid unsafe free sex attitude and the sharing of needles and syringes among intravenous drug users (IDUs). The sexual transmission of HIV has also apparently increased in Tulungagung. Commercial sex workers play a significant role in the spread of HIV in Tulungagung. People in Tulungagung have worked at other countries as Indonesian migrants. This condition can cause the increase number of HIV-1 case and the possibility of genetic variation (subtype) HIV-1 in Tulungagung. This research is aimed to analyze the subtype and to determine estimation of transmission mode on infected patient of HIV-1 and AIDS who came to Seruni clinic Dr. Iskak hospital in Tulungagung. 40 HIV?AIDSpatients were interviewed to determine the subtype and the transmission mode. The results showed that 14 of 40 plasma samples (35%) were successfully to amplified and sequenced. OverallCRF01-AE wereidentified as predominant subtype among HIV/AIDS patients in Tulungagung. Based on individual information, 31 of 40 subjects (77%) were heterosexual transmission.

THE EFFECT OF GENDARUSSIN A ISOLATES OF Justicia gendarussa Burm.f. LEAF IN REVERSE TRANSCRIPTASE IN HIBITION OF HIV TYPE I INVITRO Bambang Prajogo E. W.; Prihartini Widiyanti; Nasronudin Nasronudin; Bimo Aksono
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 5 (2015)
Publisher : Institute of Topical Disease Universitas Airlangga

Screening has been done to a few extracts from the leaves Justicia gendarussa Burm.f to see the growth rate of the virus from the blood plasma of HIV patients at Dr Soetomo Hospital. It is known that J. gendarussa leaf extract inhibits HIV type 1 reverse transcriptase. In addition, its main content is gendarussin A, besides gendarussin B, JGF1, JGF2 and JGF3, which have just identified. At the beginning, extraction and fractionation were performed with 3 models that highlight the absolute methanol, 70% methanol and 70% ethanol with the release of alkaloids. Furthermore, samples of each fraction were incubated in plasma of HIV patients with a titer of 3.6 10^6 copies for 1 h in concentrations of 1.64 ppm, 4.1 ppm, 8.2 ppm, 16.4 ppm and 41.0 ppm. After incubation, examination was performed by using Nucli sens a machine, which is a combination of PCR and Elisa, thus avoiding direct contact with the highly pathogenic virus. The result showed that the activity sequence from the most potential to the weak, among others, was 1.64 ppm >4.1 ppm > 8.2 ppm > 16.4 ppm > 41.0 ppm, each with barriers value of 0.62 10^6, 1.4 10^6, 1.6 10^6, 2.4 10 cells/ml. In conclusion, highest anti-HIV activity comes from the concentration of gendarussin A isolate at 1.64 ppm. Furthermore, after linearregression of y = -3.063 x + 81.37 was done, the IC50 of 10.24 ppm was obtained.

PROFILE OF HEMATOCRIT LEVEL CAPTURED BY DIGITAL HEMATOCRIT TEST Prihartini Widiyanti; Tri Arif Sardjono
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 6 (2015)
Publisher : Institute of Topical Disease Universitas Airlangga

The dengue fever is a disease caused by Dengue virus which is transmitted via Aedes aegypti and Aedes albopictus vector. This Dengue Haemorrhagic Fever (DHF) case in Indonesia tend to rise from year to year caused by delayed detection and inadequate handling. The laboratory parameter of hematocrite had regularly been performed using invasive method by taking the blood from the patient. This method is still not been able to monitor patients with DHF by repetitive and accurate measurament. This research project aims is to perform a digital hematocrit test (DHT) with non-invasive accurate sensors. Digital Hematocrit Test (DHT) is needed to presenting fast, exact, economical and accurate detection methods of hematocrit level. Measureable magnitude by the instrumentation is non-absorb intensity electromagnetic waves 560 nm emitted by transmitter captured by receiver. Signal captured by the receiver then converted into electrical signal. The electrical signal from receiver was the levels of hemoglobin. Levels of hemoglobin then converted to hematocrit. Hematokrit is three times the level of hemoglobin. Technology of hematocrit monitoring is aimed to control DHF patient clinical symptoms continuously and acquisitively.

EFFECT OF FREE ALKALOID AND NON-FREE ALKALOID ETHANOL 70% EXTRACT OF JUSTICIA GENDARUSSA BURM F. LEAVES AGAINST REVERSE TRANSCRIPTASE HIV ENZYME IN VITRO AND CHEMICAL COMPOUND ANALYSIS Bambang Prajogo; Prihartini Widiyanti; Hafrizal Riza
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 1 (2016)
Publisher : Institute of Topical Disease Universitas Airlangga

HIV-AIDS is a global problem and the deadliest disease in the world. One of HIV and AIDS prevention strategy can be done with traditional medicine research program from natural resource that has anti-HIV AIDS activity. It has been found that 70% ethanol extract of Justicia gendarussa Burm.f leaves, alkaloid free and alkaloid non-free, has a strong inhibitory activity against HIV reverse transcriptase enzyme, as an effort to find a solution in the face of HIV AIDS prevalence that is still high with problem of HIV-AIDS treatment such as side effects and resistances. Justicia gendarussa had already known for having an effect anti-HIV and therefore we were looking at the mechanism of inhibition of HIV Reverse Transcriptase enzyme. Both types of extracts were tested in vitro using ELISA technique and analysed chemical content of Gendarusin A as anti-HIV using high performance liquid chromatography. ELISA test results obtained percent inhibition, respectively for 254.2, 254.2, 235.6, and 279.7 for the concentration of 5 ppm, 10 ppm, 15 ppm and 20 ppm of free alkaloid extract and 169.0, 164.0, 130.5 and 369.5 for the concentration of 5 ppm, 10 ppm, 15 ppm and 20 ppm of non-free-alkaloid extract. The results of high performance liquid chromatography obtained Gendarusin A in the free alkaloid extract at retention time 8.402 minutes and non-free alkaloid extract at retention time 8.381. Therefore, these results concluded that the Justicia gendarussa Burm.f can be a useful resource for the isolation and development of new anti-HIV.

CYTOTOXICITY OF JUSTICIA GENDARUSSA BURM F. LEAF EXTRACTS ON MOLT-4 CELL Prihartini Widiyanti; Bambang Prajogo; Ni Putu Ermi Hikmawati
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 1 (2016)
Publisher : Institute of Topical Disease Universitas Airlangga

Justicia gendarussa Burm f. (Acanthaceae) is known for its activity as a male contraceptive and anti-HIV properties. The present study was designed to evaluate extracts of J. gendarussa for cytotoxicity activity against MOLT-4 cells. The cytotoxic activity of the fractionated-extract and 70% ethanol extracts of J. gendarussa leaves on MOLT-4 cells were evaluated using a WST-1 assay. The treatment cells, control cells without treatment and control media were also tested in duplicate. The absorbance was measured at a wavelength of 450 nm using a microplate absorbance reader (Bio-Rad). The average absorbance measures formazan produced by viable cells that metabolize the WST-1 reagent. Then the data was analyzed with regression analysis Microsoft Excel 2007 program to determine the concentration with 50% cell viability (50% Cytotoxicity Concentration, CC50). The CC50 values of the fractionated-extract and 70% ethanol extract of J. gendarussa leaves were 94 μg/ml and 78 μg/ml, respectively. The cytotoxicity of fractionated-extract and 70% ethanol extract of J. gendarussa leaves were not significantly different (p > 0.05). It can be concluded that the fractionated-extract and 70% ethanol extract of J. gendarussa leaves are not toxic to MOLT-4 cells.

ANALYSI S ON SECONDARY I NFECTI ON- TRI GGERI NG MICROORGANISMS IN HIV/AIDS PATIENTS AS A MODEL FOR POLICY CONTROL Retno Pudji Rahayu; Nasronudin Nasronudin; Retno Indrawati; Prihartini Widiyanti; Bimo Dwi Lukito; Ferdiansyah Ferdiansyah; Siti Qomariyah Khairunisa; Adiana Mutamsari; Tomohiro Kotaki
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 4 (2015)
Publisher : Institute of Topical Disease Universitas Airlangga

HIV infection is associated with immune-compromised and rising in opportunistic infection (secondary infection). Therefore, the number of mortality caused by HIV/AIDS is increasing. The use of ARV and development of HIV/AIDS management are expected to suppress the progress of HIV infection into AIDS and, therefore, the mortality can be diminished, while in fact most of the patients eventually suffer from AIDS due to secondary infection that commonly causes death. There should be a management by analysing microorganisms that trigger secondary infection. The method of this study was observational descriptive with cross sectional design. HIV infected blood samples were using ELISA Antibody (IgG and IgM) and Polymerase Chain Reaction (PCR) on laboratory test. The result showed correlation between HIV/AIDS severity and the amount and types of secondary infection. The most common secondary infections were toxoplasm (96.77%), hepatitis C (22.58%), tuberculosis (19.35%), and hepatitis B (3.22%). Other less frequent secondary infections, which were quite difficult to diagnose and not commonly found in Indonesia, were West Nile Virus (25.81%), Japanese Encephalitis Virus (3.22%), and Enterovirus (3.22%). Due to MDGs (Millenium Development Goals) target and the results above, researchers are highly demanded to contribute in decreasing mortality related to AIDS through early detection of secondary infection,including type of infection which have not been commonly found in Indonesia, such as West Nile Virus and Nipah Virus. The discovery of secondary infection in this study was not enough to suppress the occurrence of infection in HIV/AIDS patients. Antimicrobes and good nutrition are required. Moreover, there should be either a primary or secondary prophylaxis to prevent secondary infection that raises the number of mortality and morbidity of HIV/AIDS patients. The result of this study was to meet the target of MDGs by establishing new policies in handling HIV/AIDS infections and have potential as model for policy control in HIV/AIDS.

Co-Authors - Aminatun - Siswanto Achmad Ardianto Achmad Ardianto, Achmad Adanti Wido Paramadini Adiana Mutamsari Witaningrum Adri Supardi Agung Prabowo Ahmad Fadli Akfiny Hasdi Aimon Akhmad Jayadi Akhmad Jayadi Alfian Pramudita Putra Andhi Baskoro Andini Isfandiary Anggraini Barlian Arie Wibowo Bambang Prajogo Bambang Prajogo Eko Wardoyo Bella Wahyu Sukma Wati Bimo Aksono Bimo Aksono Bimo Dwi Lukito Bimo Dwi Lukito, Bimo Dwi Bramantono - Budi Utomo Budi Utomo Cindy Narita Kusuma Astuti DENI NOVIANA Dina Kartika Putri Dio Nurdin Setiawan Dio Nurdin Setiawan, Dio Nurdin Dita Ayu Mayasari, Dita Ayu Djony Izak Rudyardjo Dyar Sasmito Dyar Sasmito, Dyar Eko Budi Kuntjoro Eko Budi Kuntjoro, Eko Budi Endah Purwanti Fathan Aditya Sanjaya Fatkhunisa Rahmawati Fatkhunisa Rahmawati Fatkhunisa Rahmawati, Fatkhunisa Fa’ilal Chusna Fendy Purwanda Fendy Purwanda, Fendy Ferdiansyah Ferdiansyah Ferdiansyah Ferdiansyah Fitriyatul Qulub Fitriyatul Qulub Fitriyatul Qulub Fitriyatul Qulub Fitriyatul Qulub Franky Arisgraha Franky Arisgraha, Franky Gadis Meinar Sari Gadis Meinar Sari Ganis Tjahjono Hafrizal Riza Hafrizal Riza, Hafrizal Hajria Jabbar Helmi Majid Ar Rasyid Hendita Nur Maulida Heni Ismawati Heri Purnobasuki Heri Purnobasuki, Heri Herry Wibowo Jan Ady Jan Ady Julieta Kartikasari Juniastuti Juniastuti Juniastuti Juniastuti Kholifatul Wanda Putri Kholifatul Wanda Putri, Kholifatul Wanda Kirana Anugerah Putri Ludita Woro Indrio M. Qushay M. Qushay, M. Maria Inge Lusida Maria Inge Lusida Mirzaq Hussein Anwar Mirzaq Hussein Anwar, Mirzaq Hussein Muhammad Bagas Ananda Muhammad Vitanata Arfijanto Nabila Safira Khairina Nasronudin Nasronudin Nasronudin Nasronudin Nasronudin Nasronudin Nasronudin Nasronudin Nasronudin Nasronudin Ni Putu Ermi Hikmawati Ni Putu Ermi Hikmawati, Ni Putu Ermi Nilna Faizah Fiddarain Nilna Faizah Fiddarain, Nilna Faizah Radicha Arnanda Putri Indrawati Retna Apsari Retno Indrawati Roestamadji Retno Pudji Rahayu Satrio Adhitioso Satrio Adhitioso Sherina Pradita Eka Wahyudi Siswanto Siswanto Siswanto Siti Irma Mashitah Siti Qamariyah Khairunisa Siti Qamariyah Khairunisa, Siti Qamariyah Siti Qomariyah Khairunisa Siti Qomariyah Khairunisa, Siti Qomariyah Tami Bachrurozy Tarissa Diandra Putri Wibowo Tia Rahayu Wijayanti Tomohiro Kotaki Tomohiro Kotaki Tomohiro Kotaki, Tomohiro Tri Arif Sardjono Tri Arif Sardjono, Tri Arif Tria Refi Indriani Veren Audia Nurmansya Wulandari Prima Devi Yufan Fibriawan Yufan Fibriawan, Yufan

Title

Found 43 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 43 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 43 Documents
Search