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Journal : The Indonesian Biomedical Journal

Immunomodulatory Effect of Momordica charantia L. Fruit Ethanol Extract on Phagocytic Activity and Capacity of Mice Peritoneal Macrophages Parawansah Parawansah; Tomy Nurtamin; Sufiah Asri Mulyawati; Nuralifah Nuralifah; Wa Ode Arlina Misnaeni
The Indonesian Biomedical Journal Vol 10, No 2 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i2.390

Abstract

BACKGROUND: The purpose of this research is to understand the secondary metabolites of Momordica charantia L. extract, as well as to disclose the potential of M. charantia extract in the phagocytic activity and capacity of peritoneummacrophages.METHODS: Examination of immunomodulatory effect was done by giving M. charantia ethanol extract on 5 treatment groups, given intra-peritoneally to mice daily. Echinacea extract as positive control and double distilled water as negative control were also given. On the 8th day, mice were infected with Staphylococcus epidermidis. After 30 minutes, peritoneum fluid was obtained to observe the activity and capacity of macrophage cells.RESULTS: The results showed significant phagocytic activity (p<0.05) at a concentration of 1,200 ppm compare to the other groups. Meanwhile the macrophage cell capacity was found statistically insignificant (p>0.05). The highest phagocytic activity was the group treated with 1,200 ppm (62%), significantly higher than other groups.CONCLUSION: The secondary metabolite content of M. charantia is alkaloids, flavonoids, tannins, saponins, and triterpenoids. The 1,200 ppm M. charantia ethanol extract is potential in inducing phagocytic activity and capacity. These results indicate that the M. charantia can be suggested as a natural immunomodulator.KEYWORDS: pare fruit, Momordica charantia L., phagocytosis, macrophage, immunomodulator
Momordica charantia L. Fruit Fractions inhibit Malondialdehyde Level and Regenerate Hepatic Damage of Hyperglycemic Rats Parawansah Parawansah; I Putu Sudayasa; Andi Noor Kholidha Syarifin; Amirudin Eso; Nuralifah Nuralifah; Wa Ode Siti Rahayu Fathanah; Ferry Sandra
The Indonesian Biomedical Journal Vol 12, No 1 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i1.963

Abstract

BACKGROUND: Chronic hyperglycemia causes an increase of free radical production and in longterm, the hyperglycemia increases oxidative stress. Among medicinal plants, Momordica charantia L. fruit has been known to overcome hyperglycemia. However, role of M. charantia L. fruit on oxidative stress is not well understood. Therefore, current study was conducted to investigate the effect of M. charantia L. fruit extract on malondialdehyde (MDA) level and hepatic damage in hyperglicemic rat model.METHODS: Twenty five white rats (Rattus novergicus) were induced with Streptozotocin (STZ) and treated with/without glibenclamide, sodium carboxymethyl cellulose (Na-CMC), or M. charantia L. fruit ethanol/ethyl acetate/ n-hexane fraction. After the treatment, rat’s livers were collected and separated for histopathological examination and MDA analysis.RESULTS: The MDA level average of rats before the STZ induction was 1.37 μg/mL. MDA level average was markedly increased (23.85 μg/mL) in rats induced with STZ and treated with Na-CMC merely. The MDA level average of STZ-induced glibenclamide-treated rats was 3.12 μg/mL. Meanwhile, the MDA level averages of STZ-induced M. charantia L. fruit ethanol, ethyl acetate and n-hexane fractions-treated rats were 14.95, 8.98 and 5.37 μg/mL, respectively. The histopathology results of this study showed that adipocytes, dilated sinusoids and central vein thickening were mostly observed in STZ-induced Na-CMC-treated rats. Meanwhile, the STZ-induced ethanol/ethyl acetate/n-hexane fraction-treated rats did not exhibitthose expressions.CONCLUSION: M. charantia L. fruit fractions inhibit the MDA level average in liver tissue and regenerate hepatic damage of STZ-induced rats, especially the n-hexane fraction which could be a potential hepatic antioxidant and regenerative agent.KEYWORDS: Momordica charantia L., malondialdehyde, oxidative stress, hyperglycemia, diabetes mellitus
Inhibition of Xanthine Oxidase Activity by Ethanolic Extract of Piperomia pellucida L., Acacypha indica L. and Momordica charantia L. Parawansah Parawansah; Nuralifah Nuralifah; Gemini Alam; Rosdiana Natzir
The Indonesian Biomedical Journal Vol 8, No 3 (2016)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v8i3.194

Abstract

BACKGROUND: Uric acid is a final result of purine catabolism, the enzymatic reactions in the body cells from amino acids or ribonucleotide dinucleotide. Peperomia pellucida L. (P. pellucida), Acalypha indica L. (A. indica) and Momordica charantia L. (M. charantia) are plants which have efficacy to reduce levels of uric acid excess. The aim of this research is to find out the effect of ethanol extract of P. pellucida, A. indica and M. charantia in preventing the formation of uric acid excess by inhibiting the action of the enzyme xanthine oxidase and comparing the inhibition activity of xanthine oxidase on treatments.METHODS: The study design is experimental and conducted using the enzyme xanthine oxidase, xanthine (substrate), pH 7.5 phosphate buffer, samples (P. pellucida, A. indica and M. charantia ethanol extracts) and HCL as reaction breaker. Inhibition of xanthine oxidase was determined enzymatically and unreacted xanthine was measured by UV spectrophotometer at 290 nm. The data were expressed as percent inhibition and the inhibitory concentration (IC)50 were determined using linear regresion of inhibition activity vs. concentration.RESULTS: The IC50 of P. pellucida, A. indica and M. charantia ethanol extracts in inhibiting xanthine oxidase were 19.5 ppm, 77.6 ppm and 17.8 ppm, respectively. IC50 of allopurinol was 1.99 μg/ml, and negative control (combination of enzyme and substrate) has absorbance value of 0.75026.CONCLUSION: Ethanol extract of M. charantia showed the most potent inhibition toward xanthine oxidase compared to the other two extracts.KEYWORDS: xanthine oxidase, Peperomia pellucida L., Acalypha indica L., Momordica charantia L.
Co-Authors Akib, Nurlliyin Alya Zuhriyah Amirudin Eso Andi Noor Kholidha Andi Noor Kholidha Andi Noor Kholidha Syarifin Antrie, Geong Arba, Muhammad Arfan Arfan Ariani, Eno Retno Arismawati, A Armadany, Fery Indradewi Asriulah Jabar Damu, Rusliati Eso, Amiruddin Fadhliyah Malik Feri Indradewi Ferry Sandra Fery Indradewi Armadani Fery Indradewi Armadany Fitrawan, L.O.M. Fitrawan, La Ode Muhammad Fitriani Sonaru Gemini Alam Hasniana Nur Hasniar, Wa Ode Helma Yanda Serah I Putu Sudayasa I Putu Sudayasa Iko, Ria Agus Indah Amalia Lestari Indah Purnamasari Irma Irvan Anwar Jabbar, Asriullah Jamsir, Asmaidah Karmilah Kasmawati, Henny La Ode Muhammad Fitrawan Loly Subhiaty Idrus Mardiani, Siti Mesi Leorita, Mesi Mesrawati Trisetya Muhammad Hajrul Malaka Muhammad Ilyas Y Muhammad Ilyas Yusuf Mulyawati, Sufiah Asri Munasari, Dian Murniaty Mursyidah Apriatin Muslihin, Sari Melyana Ni Nyoman Fitri Astari Ningsih, Warda Ayu Nining Yulianti Nur illiyyin Akib Nurrokhmadhani, Wa Ode Sitti Nurul Febrina Rahmawati Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah Parawansah, Parawansah Parawansah, P Parawansah, P Pratiwi, Aulif Puteri Febriyanthi Rachman Rahmat Ramadhan Rahmawati Rahmawati Rifa’atul Mahmudah Rini, Risky Sesa Rosdiana Natzir Sabarudin Sabarudin Sabarudin, S Satriana Nasrun Septiyana, Wanda Sitti Fazrianti Saputri Sofianti Tarta Sri oktaviani Sudiman, Aswan Sunandar Ihsan Sunandar Ihsan, Sunandar Syasna, Annisa Tomy Nurtamin Ulan Dwi Shintia Wa Ode Arlina Misnaeni Wa Ode Nurmayanti Wa Ode Siti Rahayu Fathanah Wa Ode Sitti Asfiah Udu, Wa Ode Sitti Asfiah Wa Ode Tika Ertia Wahid Wahyuni Wahyuni Yulfa Yulfa