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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 19 Documents
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Search results for , issue " Vol 8, No 3 (2016): December 2016" : 19 Documents clear
Stimulation of Deprivation Cycles with Spirulina platensis Feed Supplementation on Osphronemus gouramy Physiological Responses Simanjuntak, Sorta Basar Ida; Wibowo, Eko Setio; Indarmawan, Indarmawan
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.7274

Abstract

Spirulina platensis is a phytoplankton, the cell wall composed of complex sugars so easily digested by fish. The present study was carried out to investigate stimulation cycle of feed deprivation with feed supplemented S. platensis the best to increase growth, hematological and body composition of gurami (Osphronemus gouramy). Groups of 24 fish, each in triplicate, were exposed to four different treatment for a period of 56 days. Sample measurements of growth done every 14 days, hematological and body composition measurements carried out at the end of the experiment. Growth was significantly different between stimulation cycle of feed deprivation and the control (P<0.05). Conclusions result showed that stimulation cycles of feed deprivation could not improve growth and hematological, but could improve body composition. Feed deprivation is done to reduce the cost of production, high production costs due to high feed prices. During research on feed deprivation is done by giving commercial feed, this study is to provide feed supplementation S. platensis. Thus, the results of this study can be useful for science as S. platensis information can be used as a food supplement and and for the people cultivating gurami should be fed daily supplementation of S. platensis.How to CiteSimanjuntak, S. B. I., Wibowo, E. S. & Indarmawan, I. (2016). Stimulation of Deprivation Cycles with Spirulina platensis Feed Supplementation on Osphronemus gouramy Physiological Responses. Biosaintifika: Journal of Biology & Biology Education, 8(3), 378-385.
The Effect of Bacteria Colony Pseudomonas fluorescens (UB_Pf1) and Bacillus subtilis (UB_Bs1) on the Mortality of Pratylenchus coffeae (Tylenchida: Pratylenchidae) Purwaningtyas, Presti Mardiyani; Rahardjo, Bambang Tri; Tarno, Hagus
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.5067

Abstract

Parasitic Root-Lession nematode of Pratylenchus coffeae can reduce the Indonesian coffee plants productivity. Several studies reported that Pseudomonas fluorescens and Bacillus subtilis endophytic bacteria were antagonistic bacteria to nematode. The objective of this research was to reveal the effectiveness of bacterial colonies density of P. fluorescens (UB_Pf1), B.subtilis (UB BS1), and a combination of both bacteria on nematode mortality using median lethal concentration (LC50) and median lethal time 50 (LT50). The densities of bacteria used in this study were 107, 109, 1011 and 1013 cfu/ml. 35 testing nematodes were used and the mortality was counted at 6, 12, 24, 36, and 48 hours after treatments. The results showed that LC50 values of P. fluorescens was (UB_Pf1) was 4,3x108 cfu/ml, LC50 B. subtilis (UB_Bs1) was 1,9x109cfu/ ml, and LC50 combination of both bacteria was, 8x107 cfu/ml. It implies that the application of the combination of both bacteria are more pathogenic than single bacterial treatment. The results also showed that the highest LT50 value was 13.21 hours combination of bacterial colonies with a density of 1013 cfu/ml and the lowest LT50 value was 52.00 hours on P. fluorescens (UB_Pf1) treatment with colonies density of 107 cfu/ml.How to CitePurwaningtyas, P. M., Rahardjo, B. T., & Tarno, H. (2016). The Effect of Bacteria Colony Pseudomonas fluorescens (UB_Pf1) and Bacillus subtilis (UB_Bs1) on the Mortality of Pratylenchus coffeae (Tylenchida: Pratylenchidae). Biosaintifika: Journal of Biology & Biology Education, 8(3), 286-293.
Correlation between Food Intake and Health Status with the Nutritional Status of School Children Age 9-11 in Semarang City Ali, Aiman Farag Mohammed; Muis, Siti Fatimah; Suhartono, Suhartono
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6488

Abstract

Malnutrition, a major risk factor for a number of infectious diseases, including acute upper respiratory tract infections (AURTI), is common in developing countries. Nutritional status is an important index of the quality of life. Objectives:To analyze the correlation between food intake and health status to nutritional status of 9-11 years old children in Semarang. The study was a correlation study carried among school children in Semarang aged 9-11 years old. Data are presented in the descriptive analyses and Spearman correlation. Overall, food intake (energy and protein) of 9-11 years old children in Semarang is normal with ? 90% RDA, health status of them was satisfactory (very low AURTI incidence),and their nutritional status were mostly normal. There was a correlation between energy intake with nutritional status with indicators BMI, and z-score of W/A and H/A, but there was no correlation between protein intake and AURTI with nutritional status. Energy and food intake of the children correlate with all nutritional status being studied. It should be suggested to parents to implement balanced diet, to avoid the development of obesity among elementary school children through nutrition education to prevent malnutrition as well as obesity.How to CiteAli, A. F. M., Muis, S. F., & Suhartono, S. (2016). Correlation between Food Intake and Health Status with The Nutritional Status of School Children Age 9-11 in Semarang City. Biosaintifika: Journal of Biology & Biology Education, 8(3), 249-256.
An Effort of Mutation Breeding by Oryzalin and Gamma Rays on Wild Raspberry (Rubus sp.) in Cibodas Botanical Garden Surya, Muhammad Imam; Ismaini, Lily; Destri, Destri; Normasiwi, Suluh
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6559

Abstract

Currently, Cibodas Botanical Garden (CBG) has a domestication programme of wild raspberries (Rubus sp.) from Indonesia mountain forest. One of the activities in the domestication programme is fruit breeding. In order to improve the quality of wild raspberry, two mutagens i.e. chemical (oryzalin) and physical (gamma rays) mutagens were used in the breeding programme. Moreover, seed of wild raspberries from the collection of CBG were used in the programme of mutation breeding. The results showed that each species of wild raspberries has different response on the mutagen. Percentage of seed germination were affected by mutagen of oryzalin and gamma rays. Rubus fraxinifolius and Rubus rosifolius seeds were still able to germinate after treated by 100 M of oryzalin. In the other hand, with increase in radiation above 300 Gy a reduction in percentage of germination was observed in M1 generation of Rubus lineatus and Rubus chrysophyllus.How to CiteSurya, M. I., Ismaini, L., Destri, D. & Normasiwi, S. (2016). An Effort of Mutation Breeding by Oryzalin and Gamma Rays on Wild Raspberry (Rubus spp.) in Cibodas Botanical Garden. Biosaintifika: Journal of Biology & Biology Education, 8(3), 331-335.
Degradation of Polycyclic Aromatic Hydrocarbon Pyrene by Biosurfactant-Producing Bacteria Gordonia cholesterolivorans AMP 10 Kurniati, Tri Handayani; Rusmana, Iman; Suryani, Ani; Mubarik, Nisa Rachmania
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6448

Abstract

Pyrene degradation and biosurfactant activity by a new strain identified as Gordonia cholesterolivorans AMP 10 were studied. The strain grew well and produced effective biosurfactants in the presence of glucose, sucrose, and crude oil. The biosurfactants production was detected by the decreased surface tension of the medium and emulsification activity. Analysis of microbial growth parameters showed that AMP10 grew best at 50gmL-1pyrene concentration, leading to 96% degradation of pyrene within 7days. The result of nested PCR analysis revealed that this isolate possessed the nahAc gene which encodes dioxygenase enzyme for initial degradation of Polycyclic Aromatic Hydrocarbon (PAH). Observation of both tensio-active and emulsifying activities indicated that biosurfactants which produced by AMP 10 when grown on glucose could lower the surface tension of medium from 71.3 mN/m to 24.7 mN/m and formed a stable emulsion in used lubricant oil with an emulsificationindex (E24) of 74%. According to the results, it is suggested that the bacterial isolates G. cholesterolivorans AMP10 are suitable candidates for bioremediation of PAH-contaminated environments.How to CiteKurniati, T. H., Rusmana, I. Suryani, A. & Mubarik, N. R. (2016). Degradation of Polycyclic Aromatic Hydrocarbon Pyrene by Biosurfactant-Producing Bacteria Gordonia cholesterolivorans AMP 10. Biosaintifika: Journal of Biology & Biology Education, 8(3), 336-343.
Screening Cellulolytic Bacteria from the Digestive Tract Snail (Achatina fulica) and Test the Ability of Cellulase Activity Wijanarka, Wijanarka; Kusdiyantini, Endang; Parman, Sarjana
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.7263

Abstract

On the research of enzyme production levels observed cellulase produced by bacteria in the digestive tract of the isolation of the Snail (Achatina fulica). Isolation of bacteria based on the ability of bacteria to grow on CMC media. The purpose of this study was to determine cellulase activity by cellulolytic bacteria. Some bacterial isolates were identified as cellulolytic bacteria, they were KE-B1, KE-B2, KE-B3, KE-B4, KE-B5, and KE-B6. Isolates KE-B6 was the best isolates. Furthermore KE-B6 isolates were grown on media production to determine the pattern of growth and enzyme activity. Measurement of cell growth was conducted by inoculating starter aged 22 hours at CMC production of liquid medium. Cellulase enzyme activity measurements was performed by the DNS method. The results showed that the highest activity by new isolate bacteria KE-B6 and its value of the activity of 0.4539 U/mL, growth rate () 0.377/hour and generation time (g) 1.84 hour. This research expected cellulase of producing bacteria were easy, inexpensive and efficient. This enzyme can be used as an enzyme biolytic once expected to replace expensive commercial enzyme. The biotylic enzyme can be applied to strains improvement (protoplast fusion).How to CiteWijanarka, W., Kusdiyantini, E. & Parman, S. (2016). Screening Cellulolytic Bacteria from the Digestive Tract Snail (Achatina fulica) and Test the Ability of Cellulase Activity. Biosaintifika: Journal of Biology & Biology Education, 8(3), 386-392.
The Community Structure of Phytoplankton in Seagrass Ecosystem and its Relationship with Environmental Characterstics Setiabudi, Gede Iwan; Bengen, Dietriech G.; Effendi, Hefni; Radjasa, Ocky Karna
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6549

Abstract

The aimed of this study was to determine the plankton communities and its relationship with the chemical and physical condition in seagrass ecosystem at Pegametan Bay. The composition and abundance of plankton were observed in the sea water underneath the surface and were identified based on the guideline of Illustration of the Marine Plankton of Japan. The water quality was measured in situ using WQC HI 9829. The water sample was measured using closed reflux spectrometry for COD, TOC analyzer for DOC and APHA 2102 (4500) method for Nt and Pt. There are 27 species of plankton identified, which can be classified into three groups. Diatom group consists of 18 species with a 74.56% abundance. The non-litoral group consists of 6 species with a 23.35% abundance. Moreover, dinoflagellate group consist of 3 species with a 2.09% abundance. An abundance of plankton greater than 104 cell.L-1 was found in diatome group (Nitzschia sp., Thalassiosira sp., Chaetoceros sp., Flagillaria sp., Thalassiothrix sp., and Melosira sp.) and non-litoral group (Oscillatoria sp. and Spirogyra sp.). The abundance of those species indicated the algae bloom phenomenon. Dinophysis sp. was also identified, which was harmful algal blooms.How to CiteSetiabudi, G. I., Bengen, D. G., Effendi, H., & Radjasa, O. K. (2016). The Community Structure of Phytoplankton in Seagrass Ecosystem and its Relationship with Environmental Characterstics. Biosaintifika: Journal of Biology & Biology Education, 8(3), 257-269.
In Vitro Selection of Local Maize (Zea mays) on NaCl Stress and Its Genetic Characterization using RAPD Saputro, Triono Bagus; Finariyah, Fathin; Dianawati, Siti; Sholihah, Nur Fadlilatus; Ermavitalini, Dini
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6934

Abstract

Maize (Zea mays) is one of graminae plants that widely spread for many purposes wheter in food industry, feed, or bioenergy. Those high utilization required an increment in production, but unfortunately the needs were not meet to the demands since conversion of agricultural area increase lately. Indonesia has 99.093 km of shoreline that recognize as marginal land where the salinity is high as well. This research try to obtain tolerant variant from two local cultivars that planted in Madura Island. Manding and Talango varieties were used as an explant for callus induction stage in MS supplemented with 2.4 D. The result show that 4 ppm of 2.4 D were the best concentration to induce the callus in both varieties. The induced callus were exposed to medium MS that contain NaCl (0, 2500, 5000, and 7500 ppm). In 7500 ppm of NaCl, Manding variety has 100% of surviving callus, while Talango variety only 66.7%. Furthermore, Manding variety shows a better performance in callus weight improvement with 170 mg, while Talango shows no improvement of callus weight. The result of RAPD analysis indicate that the genome characteristic were different between initial callus and surviving callus. Out of ten primers, only five primers shows polymorphism i.e OPA 13, OPB 07, OPC 02, OPK 20, and OPU 19. Manding can be further developed as high tolerance variety in Salinity stress, thus it proposed to be developed furthermore.How to CiteSaputro, T. B., Finariyah, F., Dianawati, S., Sholihah, N. F., & Ermavitalini, D. (2016). In Vitro Selection of Local Maize (Zea mays) on NaCl Stress and Its Genetic Characterization using RAPD. Biosaintifika: Journal of Biology & Biology Education, 8(3), 344-352.
A Dermatoglyphic Study: Association of Fingerprint Patterns Among ABO Blood Groups Susmiarsih, Tri Panjiasih; Mustofa, M. Samsul; Mirfat, Mirfat
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.5189

Abstract

Fingerprints are probably the most common biometric technique used in personal identification. The potential of fingerprints to determine sex and human identification has been well exhibited. However, very few studies have been conducted correlating finger prints with blood groups. The aim of this study was to investigate the distribution of fingerprint patterns based on ABO blood groups. The total sample consisted of 302 medical students of YARSI University Jakarta comprising of 187 females and 115 males. The fingerprint patterns were classified into arches, loops (ulnar and radial), whorls. counted and comprised triradius and total ridge count. The data analysis used Chi Square and Student-T test.The study results indicated that there were fourth especially pattern type. Significantly (p<0.05), frequency of loop types (60.36%) was highest in B blood, whorl type was highest in O blood (40.45%) and arches in AB blood was higher (5.12%) as compared to other groups. Dankmeijer indices of O and AB blood were 3.78 and 11.34, respectively. There were indicated significantly (p<0.05) difference of average ridge count total among ABO blood groups. This study implied an association between dermatoglyphics and blood groups.How to CiteSusmiarsih, T. P., Mustofa, M. S., & Mirfat, M. (2016). A Dermatoglyphic Study: Association of Fingerprint Patterns Among ABO Blood Groups. Biosaintifika: Journal of Biology & Biology Education, 8(3), 294-300.

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