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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
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Articles 21 Documents
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Search results for , issue "Vol 18 No 1 (2017)" : 21 Documents clear
Polimorfisme Protein Plasma Darah pada Kelinci Rex, Lokal dan New Zealand White (POLYMORPHISM OF BLOOD PLASMA PROTEIN OF REX, LOCAL AND NEW ZEALAND WHITE RABBIT) Riri Sarfan; Sutopo Sutopo; Edy Kurnianto
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.932 KB) | DOI: 10.19087/jveteriner.2017.18.1.144

Abstract

The objective of this study was to determine genetic diversity of rabbit by blood plasma protein variance using electrophoresis at loci of pre-albumin (Pa), albumin (Alb), ceruloplasmin (Cp), transferrin (Tf), post-transferrin (P-tf) and amylase-I (Am-I). The blood analysis was performed at the Laboratory of Physiology and Biochemistry, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta. The materials used were 63 blood samples of rex, local and new zealand white (NZW) rabbit, in which the number of sample in each breed was 21, respectively. Materials were taken randomly. The protein bands resulted from electrophoresis were used to calculate the value of the gene frequency. Genetic diversity was determined by using individual heterozygosity (h) and the average heterozygosity (H) formulas. The genetic distance among breeds of rabbits was analyzed by using DISPAN and MEGA 6.06 programs. Chi-square (X2) was used to test of Hardy-Weinberg Law (HWL) equilibrium. The results showed that the loci of prealbumin (Pa), albumin (Alb), ceruloplasmin (Cp), transferrin (Tf), post-transferrin (P-tf), and amylase-I (Am-I) in rex, local and NZW rabbits were polymorphic. Rex and NZW rabbits had close genetic relationships based on phylogeny tree analysis. Chi-square test showed that rex, local, and NZW rabbits were in HWL equilibrium for locus of Pa and Cp, while at the locus of Alb, Tf, P-tf, and Am-I were in HWL unequilibrium. In conclusion, there are genetic variability of six locuses in rex, local, and NZW rabbits. ABSTRAK Tujuan penelitian ini adalah untuk mengetahui keragaman genetik kelinci melalui analisis keragaman protein plasma darah dengan metode elektroforesis pada lokus pre-albumin (Pa), albumin (Alb), ceruloplasmin (Cp), transferin (Tf), post-transferin (P-tf), dan amylase-I (Am-I). Analisis darah dilakukan di Laboratorium Fisiologi dan Biokimia, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta. Sebanyak 63 sampel darah kelinci rex, lokal dan New Zealand White (NZW) masing-masing sebanyak 21 sampel digunakan dalam penelitian ini. Penentuan kelinci dilakukan dengan metode observasional dengan cara kelinci dipilih secara acak. Pita-pita protein yang dihasilkan dari elektroforesis digunakan untuk menghitung nilai frekuensi gen. Perhitungan nilai ragam genetik ditentukan menggunakan rumus heterosigositas individual (h) dan rataan heterosigositas (H). Hubungan dan jarak genetik antar bangsa kelinci menggunakan program DISPAN dan MEGA 6.06. Analisis statistika menggunakan tabel X2 untuk menguji lokus-lokus dalam keseimbangan Hukum Hardy-Weinberg (HHW). Hasil penelitian menunjukkan bahwa lokus pre-albumin (Pa), albumin (Alb), ceruloplasmin (Cp), transferin (Tf), post-transferin (P-tf), dan amylase-I (Am-I) pada kelinci rex, lokal, dan NZW bersifat polimorfik. Kelinci rex dan NZW memiliki hubungan genetik yang dekat berdasarkan analisis pohon filogeni. Hasil uji X2 pada kelinci rex, lokal, dan NZW menunjukkan keseimbangan HHW pada lokus Pa dan Cp, sedangkan pada lokus Alb, Tf, P-tf, dan Amy-I memiliki keseimbangan HW yang berbeda-beda. Simpulan dari penelitian ini adalah terdapat keragaman genetik enam lokus pada kelinci rex, lokal, dan NZW.
Sekuen Nukleotida Gene Shiga like toxin-2 dari Isolat Lokal Escherichia coli O157:H7 asal Hewan dan Manusia (NUCLEOTIDES SQUENCES OF SHIGA-LIKE TOXIN 2 GENES OF ESCHERICHIA COLI O157:H7 LOCAL ISOLATES ORIGINATED FROM ANIMALS AND HUMAN) I Wayan Suardana; Dyah Ayu Widiasih; Komang Januartha Putra Pinatih
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (364.77 KB) | DOI: 10.19087/jveteriner.2017.18.1.83

Abstract

Animals/livestock, especially cattle, are known as the main reservoir of Escherichia coli O157: H7. As the only one of zoonotic E. coli, the pathogenicity of these bacteria is determined by its ability to produce one or more very potent cytotoxin known as Shiga-like toxin (Stx) or verocytotoxin, particularly of the Stx2 type that is closely related to the incidence of hemolytic uremic syndrome (HUS) in humans. This study analyzed the nucleotide sequences of stx2 gene between isolates from animals and humans in an effort to assess the potential zoonoses of the agent. The research activity was initiated by cultivating 20 isolates of E. coli O157:H7 collection based on result in the previous study i.e. 2 isolates originated from cattle feces, 2 isolates originated from beef, 2 isolates originated from chicken feces, 2 isolates originated from human feces, and 12 non-clinical isolates originated from human fecal who were suffering with renal failure. All isolates were confirmed on selective medium Sorbitol MacConkey Agar (SMAC) followed by testing on aglutination O157 latex test, and H7 antisera. Molecular analysis of stx2 gene covering open reading frame (ORF) of the stx2 gene was performed using the primer which was designed by researcher i.e. Stx2 (F)/Stx2 (R). The results showed, there were 2 isolates i.e. KL-48 (2) originated from human feces and SM-25 (1) originated from cattle feces were positive for carrying a stx2 gene, which was marked by the 1587 bp PCR product. Analysis of sequencing showed both isolates had identical to stx2 nucleotide squences with E. phaga 933 as well as E. coli ATCC 933. These results indicate the both local isolates are potential as zoonotic agents with clinical effects similar to E. phaga 933 and E. coli ATCC 43894. ABSTRAK Hewan ternak khususnya sapi, dikenal sebagai reservoir utama Escherichia coli O157:H7. Sebagai satu-satunya serotipe E. coli yang bersifat zoonosis, patogenitas bakteri ini ditentukan oleh kemampuannya untuk menghasilkan satu atau lebih cytotoxin yang sangat potensial yang dikenal dengan nama Shiga-like toxin (Stx) atau verocytotoxin, khususnya dari jenis Stx2 yang terkait erat dengan kejadian hemolytic uremic syndrome (HUS) pada manusia. Studi ini bertujuan menganalisis susunan nukleotida dari gen stx2 antara isolat asal hewan dan manusia dalam upaya mengkaji potensi zoonosis yang ditimbulkannya. Kegiatan penelitian diawali dengan kultivasi 20 isolat E. coli O157:H7 koleksi hasil penelitian sebelumnya dengan rincian dua isolat asal tinja sapi, dua isolat asal daging sapi, dua isolat asal tinja ayam, dua isolat asal tinja manusia non-klinis, dan 12 isolat asal tinja manusia klinis (asal penderita gagal ginjal). Isolat sebanyak 20 tersebut dikonfirmasi pada media selektif sorbitol MacConkey agar (SMAC) yang dilanjutkan dengan uji latex O157 aglutination test serta uji antiserum H7. Analisis molekuler komplit gen stx2 yang meliputi open reading frame (ORF) dari gen stx2 dilakukan menggunakan primer rancangan peneliti yaitu Stx2(F)/Stx2(R). Hasil penelitian menunjukkan bahwa ada dua isolat yaitu KL-48 (2) asal tinja manusia dan SM-25 (1) asal tinja sapi positif membawa gene stx2 yang ditandai dengan produk PCR 1587 bp. Analisis hasil sekuensing menunjukkan kedua isolat memiliki susunan gene stx2 yang identik dengan E. phaga 933 dan E. coli ATCC 43894. Hasil ini mengindikasikan kedua isolat lokal berpotensi sebagai agen zoonosis dengan efek klinis yang serupa dengan E. phaga 933 dan E. coli ATCC 43894.
Profil Imunoglobulin-G Serum Kambing Peranakan Etawah Bunting yang Diberi Imbuhan Pakan Mineral Seng (SERUM IMUNOGLOBULIN-G LEVEL ON PREGNANT ETTAWAH CROSSBRED WERE GIVEN ZINC MINERAL) Sus Derthi Widhyari; Anita Esfandiari; I Ketut Sutama; Setyo Widodo; I Wayan Teguh Wibawan; Rizal Rahadian Ramdhany
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.664 KB) | DOI: 10.19087/jveteriner.2017.18.1.24

Abstract

The objective of this study was to find out the effect of zinc supplementation on the profile of immunoglobulin-G (IgG) in the pregnant etawah-cross ewes. Fifteen etawah-cross ewes (3-6 years old and 30-50 kg body weight) were used in this experiment and they were divided into three groups each of which consisted of 5 ewes. The first group (Zn40) received 40 ppm Zn as control, the second group (Zn60) received 60 ppm Zn, and the third group (Zn80) received 80 ppm Zn. Blood samples were collected from jugular vein every two weeks, started at twelve weeks of pregnancy up to eight weeks post partum for immunoglobulin-G analysis. IgG level were analyzed by competitive Enzyme Linked Immunosorbent Assay (ELISA) technique. Results showed that there were no significant difference of immunoglobulin-G level (P>0,05) among the three treatment groups ( Zn40, Zn60, and Zn80). However, IgG level of Zn40 and Zn80 groups tended to decrease during the last staget of pregnancy until two weeks post partum. In conclusion, supplementation of 60 ppm Zn showed higher IgG level than Zn 80 ppm. ABSTRAK Penelitian ini bertujuan untuk mengetahui efek suplementasi seng (Zn) terhadap imunoglobulin-G (IgG) pada kambing peranakan etawah (PE) bunting. Penelitian ini menggunakan 15 ekor kambing PE umur sekitar tiga sampai enam tahun, bobot badan sekitar 30-50 kg dibagi ke dalam tiga kelompok, masing-masing terdiri dari lima ekor. Kelompok Zn40 (kontrol) diberi pakan mengandung mineral Zn 40 ppm, kelompok Zn60 mengandung mineral Zn 60 ppm, dan kelompok Zn80 mengandung mineral Zn 80 ppm. Sampel darah diambil melalui vena jugularis untuk menganalisis konsentrasi IgG. Pengambilan sampel darah dilakukan setiap dua minggu mulai umur kebuntingan 12 minggu sampai delapan minggu setelah melahirkan. Hasil penelitian menunjukkan bahwa konsentrasi IgG serum tidak menunjukkan perbedaan secara nyata antar kelompok (P>0,05) antara kelompok Zn 0, Zn 40 dan Zn 80 ppm. Kelompok Zn 40 ppm dan 80 ppm cendrung memperlihatkan konsentrasi IgG mengalami penurunan pada akhir kebuntingan sampai dua minggu setelah melahirkan. Pada kelompok Zn 60 ppm memperlihatkan konsentrasi IgG lebih tinggi dibanding kelompok Zn 80 ppm.
Peningkatan Ekspresi Vascular Endothel Growth Factor dan Mitogen Activating Protein Kinase Plasenta Tikus yang Dipapar Carbon Black ((INCREASING EXPRESSION ON VASCULAR ENDOTHELIAL GROWTH FACTOR AND MITOGEN ACTIVATING PROTEIN KINASE IN PLACENTA RATS EXPOSE Viski Fitri Hendrawan; Widjiati Widjiati; Suherni Susilowati; Pudji Srianto
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.745 KB) | DOI: 10.19087/jveteriner.2017.18.1.59

Abstract

Carbon black is one of the components of particulate matter (PM), known as cytotoxic, causing inflammationin the respiratory and cardiovascular system, and able to pass through the placental barrier, which may affect the fetus. The effects caused by pollutants depend on the intensity and time length of the exposure.The objective of this study is to identify Vascular Endothelial Growth Factor (VEGF) and Mitogen Activating Protein Kinase (MAPK) as an indicator of maternal placental inflammation in pregnant rats (Rattus norvegicus) exposed to carbon black with immunohistochemical technique. This study was a laboratory experiment with three treatment groups and seven replications using pregnant female white rats (Rattus norvegicus). Pregnant female rats were exposed to carbon black with a dosage of 523 mg/m3 and 1064 mg/m3 during pregnancy of day 6 up to day 11. The next phase was the observation and examination of the expressions of VEGF and MAPK by immunohistochemical staining toward placenta of the white rats exposed to carbon black. The result of the study showed an increase in the expression of VEGF in the placenta of the rats exposed to carbon black with a dosage of 523 mg/m3 and 1064 mg/m3 per inhale during the mid-period (6-11) of pregnancy. In addition, there was also an increasein the expression of MAPK on the placenta of the rats exposed to carbon black with a dosage of 523 mg/m3and 1064 mg/m3 per inhale during the mid-period (day 6-11) of pregnancy. In conclusion, the expression of VEGF and MAPK are increased along with the increased dosage of carbon black and age of gestation. ABSTRAK Carbon black merupakan salah satu komponen partikulat matter (PM) yang diketahui bersifat sitotoksik serta menyebabkan terjadinya inflamasi pada sistem pernapasan dan kardiovaskular serta dapat melewati barier plasenta fetus sehingga dapat memengaruhi fetus. Efek yang ditimbulkan oleh polutan tergantung dari besarnya paparan dan lama waktu paparan. Penelitian ini bertujuan untuk mengidentifikasi Vascular Endothel Growth Factor (VEGF) dan Mitogen Activating Protein Kinase (MAPK) sebagai indikator imflamasi pada plasenta maternal tikus (Rattus norvegicus) yang dipapar carbon black dengan teknik imunohistokimia. Penelitian ini dilakukan dengan tiga kelompok perlakuan dan tujuh kali ulangan yang menggunakan tikus betina bunting. Tikus betina bunting dipapar dengan carbon black dosis 523 mg/m3 dan 1064 mg/m3 selama kebuntingan hari ke-6 sampai 11. Tahap berikutnya adalah pengamatan dan pemeriksaan ekspresi VEGF dan MAPK dengan pewarnaan imunohistokimia terhadap plasenta tikus putih yang telah dipapar dengan carbon black. Hasil penelitian adalah terjadi peningkatan ekspresi VEGF pada plasenta tikus yang dipapar carbon black dosis 523 mg/m3 dan 1064 mg/m3 per inhalasi selama masa pertengahan (6-11) kebuntingan. Selain itu terjadi peningkatan ekspresi MAPK pada plasenta tikus yang dipapar carbon black dosis 523 mg/m3 dan 1064 mg/m3 per inhalasi selama masa pertengahan(6-11) kebuntingan. Simpulan penelitian ini adalah ekspresi VEGF dan MAPK meningkat seiring dengan peningkatan dosis carbon black dan umur kebuntingan.
Pemberian Ransum Berenergi Tinggi Memperbaiki Performans Induk dan Menambah Bobot Lahir Pedet Sapi Bali (PROVISION HIGHER LEVEL OF ENERGY RATION IMPROVE CATTLE PERFORMANCE AND CALVES BIRTH WEIGHT) Ni Nyoman Suryani; I Wayan Suarna; Ni Putu Sarini; I Gede Mahardika; Magna Anuraga Putra Duarsa
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (82.423 KB) | DOI: 10.19087/jveteriner.2017.18.1.154

Abstract

This study aimed to determine the effect of energy levels in bali cattle rations of seven months pregnant on birth weight calves. The study was conducted in Farm Sobangan Badung Regency on 12 pregnant breeding phase of pre-calving (two months before the birth) with the parent body weight at average 300 kg/head. The treatments were four types of rations which was iso protein 10% with the energy level were 2000, 2100, 2200, and 2300 kcal ME/kg respectively. Variables measured were: weight gain, consumption of dry matter (DM), organic matter (OM), consumption energy, crude protein (CP) and crude fiber (CF), and birth weight calves. The design used was a randomized block design. Results showed DM intake varied from 5175.80 to 5366.80 g/d. Consumption of OM ranging from 4438.54 to 4610.44 g/d. Calf birth weight was also highest in the parent with treatment D is 18 kg. All these differences were not statistically significant (P>0.05). Energy consumption significantly highest (P <0.05) at the treatment D i.e. 19320.65 kcal GE/d. The conclusion of this study is energizing ration of 2000–2300 kcal ME/kg increase energy consumption however, improve performance seven months pregnant bali cattle and calf birth weight to add into 18 kg. ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh level energi ransum pada sapi bali bunting tujuh bulan terhadap bobot lahir pedet. Penelitian dilakukan di Stasiun Penelitian Sobangan, Mengwi, Badung, Bali pada 12 ekor induk bunting fase pre-calving (dua bulan menjelang kelahiran) dengan bobot badan induk sekitar 300 kg/ekor. Perlakuan yang diberikan adalah empat jenis ransum iso protein 10% dengan level energi berbeda (2000, 2100, 2200, dan 2300 kkal ME/kg) sebagai perlakuan A, B, C, dan D. Peubah yang diamati: pertambahan bobot badan, konsumsi bahan kering (BK), bahan organik (BO), konsumsi energi, protein kasar (PK), serat kasar (SK), dan bobot lahir pedet. Rancangan yang digunakan adalah Rancangan Acak Kelompok. Hasil penelitian menunjukkan konsumsi BK bervariasi dari 5175,80–5366,80 g/h. Konsumsi BO mulai dari 4438,54–4610,44 g/e/h. Bobot lahir pedet juga tertinggi pada induk dengan perlakuan D yaitu 18 kg/e. Semua perbedaan ini secara statistika tidak nyata (P>0,05). Konsumsi energi nyata (P<0,05) tertinggi pada perlakuan D yaitu 19,320,65 kkal GE/h. Simpulan dari hasil penelitian ini adalah pemberian energi ransum dari 2000–2300 kkal ME/kg meningkatkan konsumsi energi, memperbaiki performans sapi bali bunting tujuh bulan, dan menambah bobott lahir pedet sehingga menjadi 18 kg.
Strategi Pengelolaan Usaha Jasa Rumah Pemotongan Hewan Ruminansia Secara Berkelanjutan (MANAGEMENT STRATEGY FOR SUSTAINABLE RUMINANT-CATTLE SLAUGHTERHOUSE (RC-S) SERVICES) Maya Dewi Dyah Maharani; Sumardjo Sumardjo; Eriyatno Eriyatno; Eko Sugeng Pribadi
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (314.608 KB) | DOI: 10.19087/jveteriner.2017.18.1.94

Abstract

Uncontrolled cattle slaughtering may result in reduce water quantity and quality, number of cattle, and product of animal origin that does not fit the criteria of safety-healthy-whole-halal. The research objective was to analyze and measure important variables which might be required in developing a modelas well as to formulate strategic plans for managing sustainable abattoir services. Analysis methods used in this study were: Multidimensional Scaling (MDS) or rap-slaughterhouse, Monte Carlo Analysis, and Analytical Hierarchy Process (AHP). Results of the analysis showed that the important variables were, i.e.: water availability and animal health status (ecological dimension); willingness to conduct hygienic and sanitation procedures at business scale level, service business orientation, availability of animal and slaughtering schedule management (economical dimension); workers safety and security (sociological dimension), noncompliance of the use of infrastructure by managers, workers and service users; lack off ulfillment of the requirements of the building frame (dimension regulations); and processing technology (technological dimension). The priority strategic analysis concluded that supply services of product of animal origin is eligible to be considered as components required in the food security program through the development and renovation program of effective slaughter houses. ABSTRAK Pemotongan hewan yang tidak terkendali mengakibatkan penurunan ketersediaan jumlah dan mutu air, hewan, dan pangan asal ternak yang tidak sesuai kriteria aman-sehat-utuh-halal (ASUH). Tujuan penelitian adalah menganalisis dan mengukur peubah-peubah penting yang diperlukan untuk menyusun model dan merancang rencana strategik pengelolaan usaha jasa rumah pemotongan hewan ruminansia secara berkelanjutan. Metode analisis yang digunakan adalah Multi Dimensional Scaling (MDS) atau rap-slaughterhouse, Monte Carlo, dan Analytical Hierarchy Process. Hasil analisis mendapatkan beberapa faktor-faktor penting, yaitu ketersediaan air bersih dan status kesehatan hewan untuk dimensi ekologi; kesediaan melaksanakan perlakuan yang higienis dan sanitasi pada tingkatan skala usaha; orientasi usaha jasa; ketersediaan hewan; pengaturan jadwal waktu pemotongan untuk dimensi ekonomi; keselamatan dan keamanan pekerja (dimensi sosial); ketidaktaatan penggunaan sarana dan prasarana oleh pengelola, pekerja dan pengguna jasa; kurang dipenuhinya persyaratan rangka bangunan (dimensi peraturan); dan teknologi pengolahan (dimensi teknologi). Strategi prioritas yang dihasilkan adalah pelayanan penyediaan pangan asal ternak layak untuk dijadikan komponen yang wajib dalam program ketahanan pangan melalui program pembangunan dan renovasi rumah pemotongan hewan ruminansia yang efektif.
Gambaran Histopatologi Toksoplasmosis pada Kucing Peliharaan (HISTOPATHOLOGICAL FEATURES OF TOXOPLASMOSIS IN DOMESTIC CAT) Muhammad Hanafiah; Wisnu Nurcahyo; Joko Prastowo; Sri Hartati
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.81 KB) | DOI: 10.19087/jveteriner.2017.18.1.11

Abstract

Study of histopathological changes of domestic cat organs which were serologically positive toxoplasmosis and laboratory infected which Toxoplasma have been undertaken. Histological section is prepared from organs including brain, liver, lung, kidney, duodenum, jejunum, ileum, and spleen then stained using hematoxylin and eosin (HE) and observed under microscope for histopathological changes. The results showed that in the serologically positive animals cell proliferation, infiltration of leucocyte and macrophage cells were observed in the ileum, whilst infiltration of eosinophil and leucocyte was seen in the kidney and liver. However, in other organ such as duodenum, jejunum, and spleen there were no changes observed. In cat experimentally infected with Toxoplasma, the infiltration of eosinophil cells were observed in the ileum and lung, while other organs such as kidney, liver, brain, jejunum, duodenum, and spleen showed no infiltration of inflammation cells. In conclusion, based on the results seropositive cat, showed proliferation of epithelial cells, leucocyte cells, and macrophage cells in the ileum, while in the lung, kidney, and liver showed infiltration of eosinophil and leucocyte. No infiltration of inflammation cells were observed in the brain, jejunum, duodenum, and spleen. ABSTRAK Penelitian mengenai histopatologi beberapa organ kucing peliharaan yang positif Toxoplasma baik secara serologi maupun yang diinfeksikan telah dilakukan. Tujuan penelitian adalah untuk mengetahui perubahan histopatologi pada organ kucing yang positif Toxoplasma. Data hasil pemeriksaan histopatologi yang terdapat pada preparat jaringan masing-masing organ dianalisis secara deskriptif dengan melihat gambaran perubahan histopatologi pada organ otak, hati, paru, ginjal, duodenum, jejenum, ileum, dan limpa. Hasil penelitian menunjukkan bahwa dengan menggunakan metode histopatologi organ kucing yang positif toksoplasmosis secara serologi teramati adanya proliferasi sel epitel, infiltrasi sel-sel leukosit dan makrofag pada ileum, ginjal, dan hati terlihat adanya infiltrasi eosinofil dan juga infiltrasi leukosit, sedangkan organ yang lain seperti jejenum, duodenum, dan limpa tidak teramati perubahan pada jaringan yang diperiksa. Sementara pada kucing yang dinfeksikan Toxoplasma, ileum dan paru teramati adanya infiltrasi sel-sel eosinofil, sedangkan organ lainnya seperti ginjal, hati, otak, jejenum, duodenum, dan limpa tidak teramati adanya infiltrasi sel-sel radang. Simpulan yang dapat ditarik adalah pada organ kucing yang positif toksoplasmosis teramati adanya proliferasi sel epitel, infiltrasi sel-sel leukosit, dan makrofag pada ileum, paru, ginjal dan hati teramati adanya infiltrasi eosinofil dan juga infiltrasi leukosit, sedangkan organ-organ lainnya seperti otak, jejenum, duodenum dan limpa tidak terlihat adanya infiltrasi sel-sel radang.
Kandungan L-3, 4-dihydroxyphenylalanine Suatu Bahan Neuroprotektif pada Biji Koro Benguk (Mucuna pruriens) Segar, Rebus, dan Tempe (L-3,4-DIHYDROXYPHENYLALANINE CONTENT AS A NEUROPROTECTIVE MATERIAL ON FRESH, COOKED AND FERMENTED OF KORO BENGUK (MUCUNA PR Tri Wahyu Pangestiningsih; Trini Susmiati; Hery Wijayanto
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (129.322 KB) | DOI: 10.19087/jveteriner.2017.18.1.116

Abstract

Indonesia is rich in flora potentially used for herbal medication. One of the potential herbal is koro benguk (Mucuna pruriens) beans, where in Central Java and Yogyakarta is proccessed into tempe (fermented mucuna beans) for daily human consumption. Koro benguk has high level of L-3,4-dihydroxyphenylalanine (L-DOPA) which has a potential neuroprotective effect on Parkinson’s disease. The aim of this study was to investigate the L-DOPA content in fresh beans, cooked and fermented of koro benguk beans. The investigation were done in fresh mucuna beans, white color (BR D) and black color (BR A) beans originated from Wonogiri, Central Java, and fresh, white color (KP C), cooked, and fermented beans collected from Kulon Progo, Yogyakarta. The samples were extracted using ethanol and n-propanol solutions and were analyzed using high-performance liquid chromatography (HPLC) technique. The results show that the highest L-DOPA level (8,56%) was found in fresh white koro benguk beans from Wonogiri extracted using ethanol. The lowest L-DOPA level (0,016%) was found in fermented beans that extracted using n-propanol. Extraction using ethanol yield a higher L-DOPA level as compared to that of using n-propanol. In brief, all of the samples starting from fresh bean, cooked, and fermented koro benguk beans contain L-DOPA, with highest L-DOPA level was found in the white fresh koro benguk beans, from Wonogiri, Central Java. The lowest ingredient L-DOPA level was found in the fermented beans from Kulon Progo, Yogyakarta. ABSTRAK Indonesia sangat kaya dengan keanekaragaman flora yang potensial untuk terapi herbal, salah satunya tanaman koro benguk (Mucuna pruriens) yang bijinya bisa diolah menjadi tempe sebagai konsumsi harian masyarakat di sekitar Yogyakarta dan Jawa Tengah. Biji koro benguk diketahui mengandung L-3,4-dihydroxyphenylalanine (L-DOPA) tinggi dan berpotensi menjadi agen neuroprotektor pada penyakit Parkinson. Penelitian ini bertujuan untuk menguji kandungan L-DOPA mulai dari biji koro benguk segar, rebus, dan bahan olahannya yaitu tempe benguk. Uji dilakukan pada biji koro benguk mentah kulit berwarna putih (BR D) dan hitam (BR A) asal Wonogiri, Jawa Tengah, serta biji koro benguk mentah kulit berwarna putih (KP C), biji koro benguk yang sudah direbus dua kali, dan tempe benguk asal Kulon Progo, Yogyakarta. Sampel diekstraksi menggunakan pelarut etanol dan n-propanol, kemudian dianalisis dengan teknik high-performance liquid chromatography (HPLC) untuk melihat kadar kandungan L-DOPA-nya. Hasil penelitian menunjukkan, kadar L-DOPA tertinggi (8,56%) ditemukan pada biji koro benguk mentah dengan warna kulit putih asal Wonogiri yang diekstraksi menggunakan pelarut etanol, sedangkan kadar L-DOPA terendah (0,016%) ditemukan pada sediaan tempe yang diekstraksi dengan n-propanol asal Kulon Progo. Secara umum, ekstraksi menggunakan pelarut etanol memberikan hasil kadar L-DOPA yang lebih tinggi dibandingkan dengan pelarut n-propanol. Semua ekstraksi sampel, mulai biji koro benguk segar, rebus sampai bentuk tempenya mengandung L-DOPA, dengan kadar tertinggi terdapat pada biji koro benguk segar berkulit putih asal Wonogiri, Jawa Tengah yang diekstraksi menggunakan etanol, sedangkan kadar terendah dijumpai pada tempe benguk dari Kulon Progo, Yogyakarta yang diekstraksi menggunakan n-propanol.
Gambaran Biokimia dan Leukosit Darah Ayam Kampung Umur 25 Hari yang Diberi Probiotik Fungi Rhizopus oryzae (BIOCHEMICAL AND BLOOD LEUKOCYTES DESCRIPTIONS OF 25 DAYS AGE OF KAMPONG CHICKEN FEEDWITH FUNGI Rhizopusoryzae) Isroli Isroli; Turrini Yudiarti; Sugiharto Sugiharto
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (90.81 KB) | DOI: 10.19087/jveteriner.2017.18.1.46

Abstract

The aim of this present study was to investigate the effect of dietary supplementation of fungus probiotic Rhizopus oryzae on the serum biochemical and leukocytes profile of 25 days old kampong chicken. A total of 100 day-old chicks weight 39.65 ± 1.46 g were randomly allotted into 20 plots of enclosure. All chickens received feed containing 20.54% crude protein, metabolism energi of 2913 kcal/kg ad libitum. Acompletely randomized experimental design with 4 treatments and five replicates, each replicate of 5 chicken was applied. The treatments were: supplementation of fungus probiotic R. oryzae in the diets (atthe levels of 0% (T0), 0.2% (T1), 0.4% (T2), and 0.6% (T3), respectively. The treatment diets were given at days 1, 7, 14, and 21, respectively. Parameters measured including blood biochemistry (uric acid, total protein, albumin, and globulin) and leukocyte profile (total leukocytes and its differentials). Results showed that dietary supplementation of probiotics R. oryzae had no effect on the parameters observed, except for the percentage of eosinophils. It can be concluded that the administration of R. oryzae in the ration at every seven days intervals failed to improve the physiological condition of the kampong chickens. ABSTRAK Mikrobiota yang sering digunakan sebagai probiotik pada umumnya bakteri. Namun fungi juga berpotensi sebagai probitik. Ayam kampung perlu ditingkatkan kesehatan dan produktivitasnya yang rendah melalui penggunaan probiotik. Sebanyak 100 ekor ayam kampung umur sehari bobot badan rata-rata 39,65±1,46 gram dibagi secara acak ke dalam 20 petak kandang panggung. Ayam diberi pakan berkadar protein kasar 20,54% energi metabolisme 2913 kkal/kg secara ad libitum. Penelitian menggunakan rancangan acak lengkap dengan empat perlakuan lima ulangan, dan setiap ulangan terdiri lima ekor ayam. Perlakuan berupa penambahan probiotik fungi R. oryzae dalam pakan T0, T1, T2, dan T3 masing-masing 0,0%, 0,2%, 0,4%, dan 0,6%. Pakan yang mengandung probiotik diberikan pada hari ke 1, 7, 14, dan 21. Parameter yang diukur meliputi biokimia darah (asam urat, protein plasma total, albumin, globulin) dan leukosit (total dan diferensial). Data dianalaisis keragamannya pada taraf 5%. Hasil penelitian menunjukkan bahwa pemberian probiotik R. oryzae dalam pakan hanya berpengaruh nyata (P<0,05) pada persentase eosinofil, namun tidak berpengaruh terhadap semua parameter yang diukur. Dapat disimpulkan bahwa pemberian R. oryzae dalam pakan setiap tujuh hari tidak menyebabkan ayam kampung meningkatkan respon imunnya.
Sekuensing 16S DNA Bakteri Selulolitik Asal Limbah Cairan Rumen Sapi Peranakan Ongole (SEQUENCING OF 16S DNA OF CELLULOLYTIC BACTERIA FROM BOVINE RUMEN FLUID WASTE ONGOLE CROSSBREED) Widya Paramita Lokapirnasari; Adriana Monica Sahidu; Tri Nurhajati; Koesnoto Supranianondo; Andreas Berny Yulianto
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.233 KB) | DOI: 10.19087/jveteriner.2017.18.1.76

Abstract

This study aimed to identified cellulolytic inoculant code WPL 214 isolated from bovine rumen fluid waste of Ongole Cross Breed of Surabaya Slaughter house. A single colony of isolates celulolytic grown on 5 mL of liquid media Luria Bertani (LB) consist of 1 % NaCl , 1% tripton , 0.5 % yeast extract, containing1 % carboxymethyl cellulose (CMC) at temperature 37°C, using a shaker of incubator during 16-18 hours. That isolate determined by 16S DNA gen analysis using High Fidelity Platinum Taq DNA Polymerase with primer forward PB36 5’-AGR GTT TGA TCM TGG CTC AG-3’ and primer reverse PB38 5’-GMT ACCTTG TTA CGA CTT-3’ for PCR. Nucleotide sequence of 16S DNA fragment was determined through the sequencing method. The result was then compared with GenBank database to recognize the type of the sample bacteria. DNA isolation and 16S DNA coding genes amplification were carried out using Kit High Fidelity Platinum Taq DNA Polymerase. Afterward, BLAST was applied to identify the phylogenetic tree. The bacteria was capable of indicating the existence of clear zone in a media CMC by congo red staining. The existence of the clear zone associated with the activity of microbes to degrade cellulose. The conclusión of this research based on the results was the sequencing nucleotides genome 16S DNA showed that cellulolytic inoculant was identified as Enterobacter cloacae WPL 214. ABSTRAK Penelitian ini bertujuan untuk mengidentifikasi lebih lanjut isolat selulolitik kode WPL 214 yang telah diisolasi dari cairan rumen sapi peranakan ongole dari limbah Rumah Potong Hewan Surabaya. Koloni tunggal dari isolat selulolitik ditumbuhkan pada 5 mL media cair Luria Bertani (LB) dengan komposisisi 1% NaCl, 1% tripton, 0,5% yeast ekstrak, yang mengandung 1% substrat carboxymethyl cellulose (CMC) pada suhu 37°C, dengan pengocokan menggunakan shaker incubator selama ±16-18 jam. Penelitian ini terdiri dari dua tahap, tahap pertama dilakukan isolasi DNA, tahap kedua dilakukan identifikasi gen penyandi 16S DNA, amplifikasi DNA dengan polymerase chain reaction (PCR). Amplifikasi gen penyandi 16S DNA menggunakan Kit High Fidelity Platinum Taq DNA Polymerase dengan primer forward PB36 5’-AGR GTT TGA TCM TGG CTC AG-3’ dan primer reverse PB38 5’-GMT ACC TTG TTA CGA CTT-3’ yang digunakan untuk PCR. Hasil sekuensing nukleotida dari 16S DNA selanjutnya dibandingkan dengan urutan nukleotida dari GenBank database untuk dilakukan BLAST untuk mengidentifikasi berdasarkan pohon filogeni. Bakteri tersebut mampu menunjukkan adanya zona bening pada media Carboxymethyl cellulose (CMC) dengan pewarnaan congo red. Adanya zona bening tersebut berhubungan dengan aktivitas mikrob untuk mendegradasi selulosa. Simpulan penelitian menunjukkan bahwa berdasarkan hasil urutan nukleotida genom 16S DNA serta pohon filogeni, maka isolat selulolitik tersebut diidentifikasi sebagai Enterobacter cloacae WPL 214.

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