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Journal : Hemera Zoa

AQ-12 Application of a Multiplex PCR Assay to Detect Campylobacter fetus subspecies venerealis from Imported Bovine Preputial Samples Mazdani Daulay; Adi Komara; Novera Nirmalasanti; Siti Khadijah; . Marjono; Melyna Sandra; Muhamad Taopik; . Mukromin; . Mustamil; Rahmat Setya Adji
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Campylobacteriosis, caused by Campylobacter spp., is of considerable economic importance to the cattle industry worldwide. Campylobacter spp. were recognized as etiological agents of abortion in sheep. Campylobacter fetus subsp. fetus (Cff) causes sporadic abortion in sheep, often late in gestation, while subspecies venerealis (Cfv) is a cause of sexually transmitted bovine infertility and sporadic abortion in cattle. Various investigations have been carried out in different countries to assess the prevalence and impact of this disease. Some published results surveys are outlined in Table 1. Table 1. A summary of published data showing the prevalence of C. fetus in different countriesStudyareaSample type(s)Sample sizePrevale nce ofC. fetus (%)Diagnostic methodAustralia(1985-1986) Bulls (preputi al suction)1 008animals41 herds87% herdspositiveSerological(Fluorescentantibody test) California(United States of America)Cows40047Serological(ELISA) New ZealandCows (vaginal mucous) and bulls (preputial wash)1 230 cows(125 herds)54 bulls70% herds positiveCfv : 0Cff/othersSerological(ELISA)Bacteriological culture          Cff: Campylobacter fetus subsp. fetus, Cfv: C. fetus subsp. venerealis. According to [1], Bovine Genital Campylobacteriosis (BGC) disease was classified as 1st Group of animal quarantine disease. It is an exotic disease that was not ever detected in Indonesia. However, large scale cattle importation to Indonesia from the countries which ever reported BGC prevalence in their territories, initiating and spreading BGC will be the major threat for feedlotter or dairy farm in Indonesia. Hence, we should apply diagnostic test to detect Cfv in order to prevent the introducing the BGC to Indonesia. The aim of this study was to verify that multiplex PCR assay applicative to detect Campylobacter fetus subsp. venerealis from field samples.
Co-Authors . Marjono . Mukromin . Mustamil Ade Isma Khairani Adi Komara Aditya Mulia Sembiring Ahmad Nur Hidayat Alfredo Pahala Simanjuntak Amril Hanapi Annisa Azzahra Azrul Ali Akbar Bakti Nugraha Bismar H Ompusunggu Cindy Oktavia Siahaan Citra Aulia Amanda Daffa Dhiba Oesraini Darni Darni Dedek Esteria A S Desivaria Sandi Devina Nathania Avissa Marbun Diana Chalil Djuara P Lubis Dwi Mardhia Endah Pratiwi Munthe Facrul Rozi Fadhilah . Faqita Iqlima Putry Febrinae F. Damanik Firda Amelia Galih Dwi Martanto Hasman Hasyim Haya Zabidi hessy mayona Ilham Setya Budi Indah Budiarti Insiyah Insiyah Isal Putra Utama Iskandarini Jhoanita NF Johannes P Munthe Jufrianto Simanullang Julprida Saragih Lili Dariani Lily Fauzia Luhut Sihombing Luthfia Aina Salsabila Parinduri Mazdani Daulay Mei Mila Sifa Isnu Melyna Sandra Meneth Ginting Mori Dianto Muhamad Taopik Muhammad Nursidin Nany Suryani Noor Fajriah Novera Nirmalasanti Novita . Nursal Asbiran Oni Anita Wulandari Polis Tinus Harefa Praja Sembiring Puput Fauzah Sri Rejeki Rahmanta Ginting Rahmat Setya Adji Raisya Hasina Renata Selma Astagina Rilus Kinseng Rindu Febriyeni Utami Rini Elisabeth Pardede Rizky Romadhona Tambunan Rusmalawaty nasution Sabila Pratiwi Saidul Khudri Sakina T.A Harahap Satia Negara Lubis Sefti Dwi Mirantika Seri Rahmadani Sinar Indra Kesuma Sitti Sulaihah Syahrani Lubis Syari Syafrina TATI NURHAYATI Taufik Hilmi Tavi Supriana Tio Bella Audina Sitorus Yanti Sri Rezeki Yoga Pratama Yosita Hazrin Yuliana Salman Yunita Safitri Yusak Maryunianta Zainal Abidin Damanik Zakky Abdul Ghoniy Zulfa Harirah MS Zulfendri Zulfendri